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Dietary Supplementation of β-Carotene Reveals miRNAs Involved in the Regulation of carotenoid metabolism in Crassostrea gigas

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Journal of Ocean University of China 2024年第4期23卷 1045-1053页

作者： SUN Dongfang WAN Sai YU Hong KONG Lingfeng LI Qi Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Laoshan LaboratoryQingdao 266237China

carotenoids play crucial physiological roles in animals.A comprehensive investigation into the mechanism of carotenoid metabolism in oysters will establish a theoretical foundation for further development of its carotenoid-rich ***,the information on the function of miRNA in β-carotene metabolism in oysters is *** elucidate the mechanisms underlying miRNA regulation of carotenoid metabolism in oysters,we compared the expressions of miRNA in digestive gland tissues of Pacific oyster(Crassostrea gigas)fed with aβ-carotene supplemented diet and a normal diet,respectively.A total of 690 candidate miRNAs in the Pacific oyster digestive gland tissues were identified,including 590 known miRNAs and 111 unknown *** differentially expressed miRNAs were obtained in the carotenoid-fed and normal groups,associated to 137 differentially expressed target ***,the GO enrichment analysis revealed that the differentially expressed target genes were mainly involved in transmembrane transport *** enrichment showed that the differentially expressed target genes were involved in ABC *** of the mRNA-miRNA network revealed that novel0025 played a central role in carotenoid metabolism,and it was negatively correlated with the expression of 46 *** addition,down-regulated expression of novel0025 upregulated the expression of the lipoprotein gene LOC105342186,suggesting a potential regulatory role in carotenoid *** results provide useful information for elucidating the miRNA regulation mechanism during carotenoids metabolism in the Pacific oyster.

关键词： Crassostrea gigas β-carotene microRNA carotenoid metabolism

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The P450-type carotene hydroxylase PuCHY1 from Porphyra suggests the evolution of carotenoid metabolism in red algae

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Journal of Integrative Plant Biology 2014年第9期56卷 902-915页

作者： Li-En Yang Xing-Qi Huang Yu Hang Yin-Yin Deng Qin-Qin Lu Shan Lu State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing University Department of Biology and Food Engineering Changshu Institute of Technology Institute of Fisheries of Jiangsu Province

Carotene hydroxylases catalyze the hydroxylation of a- and β-carotene hydrocarbons into *** red algae,β-carotene is a ubiquitously distributed carotenoid,and hydroxylated carotenoids such as zeaxanthin and lutein are also ***,no enzyme with carotene hydroxylase activity had been previously identified in red ***,we report the isolation of a gene encoding a cytochrome P450-type carotene hydroxylase（PuCHY1） from Porphyra umbilicalis,a red alga with an ancient *** comparisons found PuCHY1 belongs to the CYP97 B subfamily,which has members from different photosynthetic organisms ranging from red algae to land *** complementation in Escherichia coli suggested that PuCHY1 catalyzed the conversion from β-carotene to *** we overexpressed PuCHYi in the Arabidopsis thaliana chy2 mutant,pigment analysis showed a significant accumulation of hydroxylated carotenoids,including neoxanthin,violaxanthin,and lutein in the leaves of transgenic *** results confirmed a β-hydroxylation activity of PuCHY1,and also suggested a possible ε-hydroxylation *** pigment profile and gene expression analyses of the algal thallus under high-light stress suggested that *** is unlikely to operate a partial xanthophyll cycle for photoprotection.

关键词： Bangiales carotene hydroxylase carotenoid metabolism CYP97B cytochrome P450 Porphyra umbilicalis red algae

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Epigenetic changes in the regulation of carotenoid metabolism during honeysuckle flower development

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Horticultural Plant Journal 2023年第3期9卷 577-588页

作者： Hang Yu Na Cui Kun Guo Wen Xu Haifeng Wang State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi Key Lab of Sugarcane BiologyCollege of AgricultureGuangxi UniversityNanningGuangxi 530005China Institute of Medicinal Plant Development Chinese Academy of Medical SciencesPeking Union Medical CollegeBeijing 100193China Innovation and Transformation Center of Science and Technology College of PharmacyFujian University of Traditional Chinese MedicineFuzhouFujian 350122China

Flower development is one of the most vital pathways in plant development, during which the epigenetic regulation of gene expression is essential. DNA methylation, the most conserved epigenetic modification, participates in gene expression regulation and transposable element silencing. Honeysuckle(Lonicera japonica) is an important medicinal plant renowned for its colorful and fragrant flowers. Honeysuckle flowers change color from white to gold as a result of carotenoid accumulation during development. However, the role of DNA methylation in flower color changes is not well understood in L. japonica. Here, we performed whole-genome bisulfite sequencing and transcriptome sequencing during flowering development in honeysuckle. The results showed that a decrease in the levels of genome-wide average DNA methylation during flower development and changes in DNA methylation were associated with the expression of demethylase genes. Moreover, many genes involved in carotenoid biosynthesis and degradation, such as Lj PSY1, LjPDS1, LjLCYE, and LjCCD4, have altered expression levels because of hypomethylation, indicating that DNA methylation plays an important role in flower color changes in honeysuckle. Taken together, our data provide epigenetic insights into flower development and color change in honeysuckles.

关键词： Lonicera japonica DNA methylation Epigenetic regulation Gene expression carotenoid metabolism

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