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Improvement and Application of Acute Blood Stasis Rat Model Aligned with the 3Rs (Reduction, Refinement and Replacement) of Humane Animal Experimentation

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Chinese Journal of Integrative Medicine 2020年第4期26卷 292-298页

作者： HUANG Shuai XU Feng WANG Yin-ye SHANG Ming-ying WANG Chao-qun WANG Xuan CAI Shao-qing State Key Laboratory of Natural and Biomimetic Drugs Peking UniversityBeijing(100191)China Hubei Engineering Research Center of Viral Vector Wuhan University of BioengineeringWuhan(430415)China Department of Molecular and Cellular Pharmacology School of Pharmaceutical SciencesPeking UniversityBeijing(100191)China Division of Pharmaceutical Analysis School of Pharmaceutical SciencesPeking UniversityBeijing(100191)China

Objective:To establish a novel cardiocentesis method for withdrawing venous blood from the right atrium,and to improve an acute blood stasis rat model using an ice bath and epinephrine hydrochloride(Epi)while considering the 3Rs(reduction,refinement,and replacement)of humane animal ***:An acute blood stasis model was established in male Sprague-Dawley rats by subcutaneous injection(s.c.)Epi(1.2mg/kg)administration at 0h,followed by a 5-min exposure to an ice-bath at 2h and *** administration at *** rats received physiological *** were fasted overnight and treated with Angelicae Sinensis Lateralis Radix(ASLR)and Pheretima the following *** blood was collected using our novel cardiocentesis method and used to test whole blood viscosity(WBV),prothrombin time(PT),activated partial thromboplastin time(APTT),and fibrinogen(FIB)***:The rats survived the novel cardiocentesis technique;WBV value returned to normal while hematological parameters such as hemoglobin level and red blood cell count were restored to>94%of the corresponding values in normal rats following a 14-day ***(1.2 mg/kg,s.c.)combined with a 5-min exposure to the ice bath replicated the acute blood stasis rat model and was associated with the highest WBV *** rats showing acute blood stasis,ASLR treatment[4g/(kg-d)for 8 days]decreased WBV by 9.98%,11.09%,9.34%,9.00%,7.66%,and 7.03%(P<0.05),while Pheretima treatment[2.6g/(kg?d),for 8 days]decreased WBV by 25.49%,25.94%,16.28%,17.76%,11.07%,and 7.89%(P<0.01)at shear rates of 1,3,10,30,100,and 180 s'1,***,Pheretima treatment increased APTT significantly(P<0.01).Conclusions:We presented a stable,reproducible,and improved acute blood stasis rat model,which could be applied to screen drugs for promoting blood circulation and eliminating blood stasis.

关键词： acute blood stasis rat model cardiocentesis whole blood viscosity coagulation parameters Angelicae Sinensis Lateralis Radix Pheretima Chinese medicine syndrome

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