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carbon monoxide Alleviates Wheat Seed Germination Inhibition and Counteracts Lipid Peroxidation Mediated by Salinity

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Journal of Integrative Plant Biology 2006年第10期48卷 1168-1176页

作者： Sheng Xu Zhi-Sheng Sa Ze-Yu Cao Wei Xuan Ben-Kai Huang Teng-Fang Ling Qiong-Ying Hu Wen-Biao Shen College of Life Sciences Laboratory Centre of Life Sciences Nanjing Agricultural University Nanjing 210095 China

Recently In animals, endogenous carbon monoxide （CO）, like nitric oxlde, was implicated as another Important physiological messenger or bioactive molecule. However, little information is known about the physlologlcal roles of CO in the whole plant. In the present study, we report that different concentrations of the 130 donor hematin （0.1, 1.0 and 10.0 μmol/L） alleviated wheat （Tilticum aestivum L. Yangmai 158） seed germination Inhlbltlon caused by 250 mmol/L NaCI stress In a dose-dependent manner. These responses were also proved by the addltion of different gaseous CO aqueous solutions from 0.1% to 100.0% of saturation. Among these treatments, the effect of 1.0 μmol/L hematin and 1.0% saturation of CO aqueous solution were the most obvlous. Furthermore, compared with non-hematin treatment, the degradation of storage reserves In wheat seeds was also accelerated. Time-course analyses showed that application of hematln dose-dependently Increased the activities of superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase activities, thus decreasing the lipid peroxidation In germinating wheat seed subjected to salt stress. Meanwhile, the responses of hematin were specific for CO because the addition of the CO scavenger hemoglobln （0.2 g/L） blocked the various actions of 1.0 μmol/L hematin. Taken together, the results of the present study demonstrate that CO, at a low concentration, is able to attenuate the seed germlnation Inhibition produced by salinity stress and counteract the lipid peroxidation in germinating wheat seeds.

关键词： carbon monoxide lipid peroxidation salt stress seed germination storage reserve degradation wheat

carbon monoxide Alleviates Salt-Induced Oxidative Damage in Wheat Seedling Leaves

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Journal of Integrative Plant Biology 2006年第3期48卷 249-254页

作者： Ben-Kai Huang Sheng Xu Wei Xuan Ming LI Ze-Yu Cao Kai-Li Liu Teng-Fang Ling Wen-Biao Shen College of Life Sciences Nanjing Agricultural University Nanjing 210095 China College of Sciences Nanjing Agricultural University Nanjing 210095 China

carbon monoxide （CO）, a by-product released during the degradation of heme by heme oxygenases （EC 1.***.99.3） In animals, is regarded as an important physiological messenger or bioactive molecule involved in many biological events that has been recently reported as playing a major role in mediating the cytoprotectlon against oxidant-induced lung Injury. In the present study, we first determined the protective effect of exogenous CO against salt-induced oxidative damage in wheat seedling leaves. Wheat seedlings treated with 0.01μmol/L hematin as the CO donor demonstrated significant reversal of chlorophyll decay, dry weight, and water loss induced by 300 mmol/L NaCl stress. Interestingly, the increase in lipid peroxidation observed in salt-treated leaves was reversed by 0.01μmol/L hematin treatment. Time-couree analyses showed that application of 0.01μmol/L hematln enhanced gualacol peroxidase, superoxide dismutase, ascorbate peroxidase and catalase activities in wheat seedling leaves subjected to salt stress. These effects are specific for CO because the CO scavenger hemoglobin （1.2 mg/L） blocked the actions of the CO donor hematln. However, higher concentration of the CO donor （1.0μmol/L） did not alleviate dry weight and water loss of salt-stressed wheat seedlings. These results suggest that exogenous application of low levels of a CO donor may be advantageous against salinity toxicity.

关键词： carbon monoxide lipid peroxidation oxidative stress salinity seedling leaves wheat.

carbon monoxide Releasing Molecule Accelerates Reendothelialization after Carotid Artery Balloon Injury in Rat

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Biomedical and Environmental Sciences 2015年第4期28卷 253-262页

作者： HU Qing Song CHEN Yang Xin HUANG Qing Sheng DENG Bing Qing XIE Shuang Lun WANG Jing Feng NIE Ru Qiong Department of Cardiology Sun Yat-sen Memorial Hospital Sun Yat-sen University

Objective This study was aimed to investigate the effects of carbon monoxide releasing molecule （CORM-2）, a novel carbon monoxide carrier, on the reendothelialization of carotid artery in rat endothelial denudation model. Methods Male rats subjected to carotid artery balloon injury were treated with CORM-2, inactive CORM-2 （iCORM-2） or dimethyl sulfoxide （DMSO）. The reendothelialization capacity was evaluated by Evans Blue dye and the immunostaining with anti-CD31 antibody. The number of circulating endothelial progenitor cells （EPCs） was detected by flow cytometry. The proliferation, migration, and adhesion of human umbilical vein endothelial cells （HUVECs） were assessed by using [3H]thymidine, Boyden chamber and human fibronectin respectively. The expressions of protein were detected by using western blot analysis. Results CORM-2 remarkably accelerated the re-endothelialization 5 d later and inhibited neointima formation 28 d later. In addition, the number of peripheral EPCs significantly increased in CORM-2-treated rats than that in iCORM-2 or DMSO-treated rats after 5 d later. In vitro experiments, CORM-2 significantly enhanced the proliferation, migration and adhesion of HUVECs. The levels of Akt, eNOS phosphorylation, and NO generation in HUVECs were also much higher in CORM-2 treated group. Blocking of PI3K/Akt/eNOS signaling pathway markedly suppressed the enhanced migration and adhesion of HUVECs induced by CORM-2. Conclusion CORM-2 could promote endothelial repair, and inhibit neointima formation after carotid artery balloon injury, which might be associated with the function changes of HUVECs regulated by PI3K/Akt/eNOS pathway.

关键词： carbon monoxide Reendothelialization Neointima formation Vascular injury

carbon monoxide-induced Stomatal Closure Involves Generation of Hydrogen Peroxide in Vicia faba Guard Cells

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Journal of Integrative Plant Biology 2008年第12期50卷 1539-1548页

作者： Xiao-Ping She Xi-Gui Song School of Life Sciences Shaanxi Normal University Xi'an 710062 China

Here the regulatory role of CO during stomatal movement in Vicia faba L. was surveyed. Results indicated that, like hydrogen peroxide （H2O2）, CO donor HemaUn induced stomatal closure in dose- and time-dependent manners. These responses were also proven by the addition of gaseous CO aqueous solution with different concentrations, showing the first time that CO and H2O2 exhibit the similar regulation role in the stomatal movement. Moreover, our data showed that ascorbic acid （ASA, an important reducing substrate for H2O2 removal） and diphenylene iodonium （DPI, an inhibitor of the H2O2-generating enzyme NADPH oxidase） not only reversed stomatal closure by CO, but also suppressed the H2O2 fluorescence induced by CO, implying that CO induced-stomatal closure probably involves H2O2 signal. Additionally, the CO/NO scavenger hemoglobin （Hb） and CO specific synthetic inhibitor ZnPPIX, ASA and DPI reversed the darkness-induced stomatal closure and H2O2 fluorescence. These results show that, perhaps like H2O2, the levels of CO in guard cells of V. faba are higher in the dark than in light, HO-1 and NADPH oxidase are the enzyme systems responsible for generating endogenous CO and H2O2 in darkness respectively, and that CO is involved in darkness-induced H2O2 synthesis in V. faba guard cells.

关键词： carbon monoxide guard cells hydrogen peroxide signaling stomata.

carbon monoxide： A Novel Antioxidant Against Oxidative Stress in Wheat Seedling Leaves

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Journal of Integrative Plant Biology 2007年第5期49卷 638-645页

作者： Zhi-Sheng Sa Li-Qin Huang Guo-Lin Wu Jin-Peng Ding Xiao-Yue Chen Tian Yu Ci Shi Wen-Biao Shen College of Life Sciences Life Science Laboratory Center Nanjing Agricultural University Nanjing 210095 China College of Sciences Nanjing Agricultural University Nanjing 210095 China

carbon monoxide （CO）, an endogenous signaling molecule in animals, also provides potent cytoprotective effects including attenuation of lung lipid peroxidation induced by oxidant in the mouse. Our recent work demonstrated that 0.01 μmol/L hematin （a CO donor） treatment of wheat plants alleviated salt-induced oxidative damage in seedling leaves. In this report, we further discovered that hematin pretreatment （≤ 0.1 μmol/L） could delay wheat leaf chlorophyll loss mediated by further treatment of H202 and paraquat, two reactive oxygen species （ROS） sources, in dose-and even time-dependent manners. Also, compared with the control samples, seedling leaves pretreated with 0.01 or 0.1 μmol/L hematin for 24 h exhibited lower levels of H2O2 and lipid peroxidation, as well as higher contents of chlorophyll and activities of antioxidant enzymes. Such beneficial effects exerted by hematin were mimicked by the pretreatment of antioxidant butylated hydroxytoluene （BHT）, and differentially reversed when CO scavenger hemoglobin （Hb）, or CO specific synthetic inhibitor ZnPPIX was added, respectively. Taken together, the results presented In this paper directly illustrate for the first time that CO is able to strongly protect plants from oxidative damage caused by the overproduction of ROS, and strengthens the evidence that CO is a potent antioxidant in various abiotic and biotic stresses, as similar results have been shown in animal tissues.

关键词： antioxidant carbon monoxide chlorophyll loss hydrogen peroxide oxidative stress paraquat wheat

carbon monoxide Signal Breaks Primary Seed Dormancy by Transcriptional Silence of DOG1 in Arabidopsis thaliana

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Phyton-International Journal of Experimental Botany 2020年第3期89卷 633-643页

作者： Danni He Guoli Deng Songpei Ying Wenjuan Yang Jiali Wei Ping Li Shanghai Key Laboratory of Bio-Energy Crops Research Center for Natural ProductsPlant Science CenterSchool of Life SciencesShanghai UniversityShanghai200444China

Primary seed dormancy is an adaptive strategy that prevents germination for viable seeds in harsh environment,ensuring seeds germination under favorable *** inducing seeds germination in a controllable manner is important for crop *** searching the chemicals that efficiently breaks seed dormancy is ***1 protein abundance in the freshly harvested seed is high,and its level is correlated to seed dormancy intensity,thus DOG1 is regarded as the timer to evaluate the seed dormancy *** this study,we found the carbon monoxide(CO)donor treatment,the transgenic line with high CO content,showed lower seed dormancy,while scavenging CO,or the mutant with lower CO level,presented strong primary seed dormancy,genetic analysis showed that DOG1 was targeted by CO signal and was prerequisite for CO-dependent seed dormancy ***,we found CO signal activated the expression of ERF/AP2 transcriptional factor ERF12,as well as enhanced the binding of ERF12 to the promoter of DOG1,ultimately transcriptional silence of DOG1 expression to break primary seed *** CO signal reduced the histone acetylation level at the chromatin of DOG1 locus to suppress its ***,our results revealed that CO acts as the novel regulator to suppress DOG1 expression and efficiently break primary seed dormancy through activating the negative factor ERF12.

关键词： Arabidopsis DOG1 carbon monoxide primary seeds germination

carbon monoxide Modulates Auxin Transport and Nitric Oxide Signaling in Plants under Iron Deficiency Stress

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Phyton-International Journal of Experimental Botany 2024年第1期93卷 45-61页

作者： Kaiyue Hong Yasmina Radani Waqas Ahmad Ping Li Yuming Luo Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental ProtectionHuaiyin Normal UniversityHuaian223300China School of Life Sciences Nanjing Forestry UniversityNanjing210037China School of Life Sciences Shanghai UniversityShanghai200444China

carbon monoxide(CO)and nitric oxide(NO)are signal molecules that enhance plant adaptation to environmental *** is an essential phytohormone for plant growth and *** and NO play crucial roles in modulating the plant’s response to iron *** deficiency leads to an increase in the activity of heme oxygenase(HO)and the subsequent generation of ***,it alters the polar subcellular distribution of Pin-Formed 1(PIN1)proteins,resulting in enhanced auxin *** alteration,in turn,leads to an increase in NO ***,iron deficiency enhances the activity of ferric chelate reductase(FCR),as well as the expression of the Fer-like iron deficiency-induced transcription factor 1(FIT)and the ferric reduction oxidase 2(FRO2)genes in plant *** of the long hypocotyl 1(HY1)gene,which encodes heme oxygenase,or the CO donor treatment resulted in enhanced basipetal auxin transport,higher FCR activity,and the expression of FIT and FRO2 genes under Fe ***,a potential mechanism is proposed:CO and NO interact with auxin to address iron deficiency *** alters auxin transport,enhancing its accumulation in roots and up-regulating key iron-related genes like FRO2 and *** auxin levels affect NO signaling,leading to greater sensitivity in root *** interplay promotes FCR activity,which is crucial for iron ***,these molecules enhance iron uptake and root growth,revealing a novel aspect of plant physiology in adapting to environmental stress.

关键词： carbon monoxide nitric oxide auxin iron deficiency signal molecule plants

carbon monoxide contributes to the constipating effects of granisetron in rat colon

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World Journal of Gastroenterology 2016年第42期22卷 9333-9345页

作者： Carmela Nacci Margherita Fanelli Maria Assunta Potenza Valentina Leo Monica Montagnani Maria Antonietta De Salvia Department of Biomedical Sciences and Human Oncology - Pharmacology Section Medical School University of Bari "Aldo Moro" Department of Interdisciplinary Medicine Medical School University of Bari "Aldo Moro"

AIM To investigate the mechanisms underlying the potential contribution of the heme oxygenase/carbon monoxide(HO/CO) pathway in the constipating effects of granisetron. METHODS For in vivo studies, gastrointestinal motility was evaluated in male rats acutely treated with granisetron [25, 50, 75 μg/kg/subcutaneous(sc)], zinc protoporphyrin IX [Zn PPIX, 50 μg/kg/intraperitoneal(ip)] and hemin(50 μmol/L/kg/ip), alone or in combination. For in vitro studies, the contractile neurogenic response to electrical field stimulation(EFS, 3, 5, 10 Hz, 14 V, 1 ms, pulse trains lasting 10 s), as well as the contractile myogenic response to acetylcholine(ACh, 0.1-100 μmol/L) were evaluated on colon specimens incubated with granisetron(3 μmol/L, 15 min), Zn PPIX(10 μmol/L, 60 min) or CO-releasing molecule-3(CORM-3, 100, 200, 400 μmol/L) alone or in combination. These experiments were performed under co-treatment withor without atropine(3 μmol/L, a muscarinic receptor antagonist) or NG-nitro-L-Arginine(L-NNA, 100 μmol/L, a nitric oxide synthase inhibitor).RESULTS Administration of granisetron(50, 75 μg/kg) in vivo significantly increased the time to first defecation(P = 0.045 vs vehicle-treated rats), clearly suggesting a constipating effect of this drug. Although administration of Zn PPIX or hemin alone had no effect on this gastrointestinal motility parameter, Zn PPIX co-administered with granisetron abolished the granisetron-induced constipation. On the other hand, co-administration of hemin and granisetron did not modify the increased constipation observed under granisetron alone. When administered in vitro, granisetron alone(3 μmol/L) did not significantly modify the colon's contractile response to either EFS or ACh. Incubation with Zn PPIX alone(10 μmol/L) significantly reduced the colon's contractile response to EFS(P = 0.016) but had no effect on contractile response to ACh. Co-administration of Zn PPIX and atropine(3 μmol/L) abolished the Zn PPIX-mediated decrease in contrac

关键词： Granisetron carbon monoxide Heme oxygenase Colon Contraction Neurogenic response Myogenic response

carbon monoxide Emission and Concentration Models for Chiang Mai Urban Area

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Advances in Atmospheric Sciences 2006年第6期23卷 901-908页

作者： Surachai SATHITKUNARAT Prungchan WONGWISES Rudklao PAN-ARAM 张美根 The Joint Graduate School of Energy and Environment at King Mongkut's University of Technology Thonburi Bangkok 10140Thailand Electricity Generating Authority of Thailand Nonthaburi 11130Thailand Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029

An emission inventory containing emissions from traffic and other sources was complied. Based on the analysis, carbon monoxide （CO） emissions from traffic play a very important role in CO levels in Chiang Mai area. Analysis showed that CO emissions from traffic during rush hours contributed approximately 90% of total CO emissions. Regional Atmospheric Modeling System （RAMS） was applied to simulate wind fields and temperatures in the Chiang Mai area, and eight ca^es were selected to study annual variations in wind fields and temperatures. Model results can reflect major features of wind fields and diurnal variations in temperatures. For evaluating the model performance, model results were compared with observed wind speed, wind direction and temperature, which were monitored at a meteorological tower. Comparison showed that model results are in good agreement with observations, and the model captured many of the observed features. HYbrid Particle And Concentration Transport model （HYPACT） was used to simulate CO concentration in the Chiang Mai area. Model results generally agree well with observed CO concentrations at the air quality monitoring stations, and can explain observed CO diurnal variations.

关键词： carbon monoxide emission concentration Chiang Mai RAMS HYPACT