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Asian Pacific Journal of Tropical Medicine 2011年第11期4卷 883-888页

作者： Rajesh J Mandade SA Sreenivas DM Sakarkar Avijit Choudhury Pharmacology Department S.N.Institute of PharmacyPusadIndia Guru Nanak Institute of Pharmacy IbrahimpattnamHyderabad.(A.P.) India S.N.Institute of Pharmacy PusadIndia Pharmacology Department Shree Dhanvantary college pharmacyKimIndia

Objective:To explore the effects of extract of Hibiscus rosasinensis(***) on the volume,free and total acidity of gastric secretion induced by ***:Animals were kept on fasting for 48 *** the pylorus of each animal was *** were randomly divided into 5 groups and treated by carbachol at 600μg/*** animals in groupⅡ-Ⅴwere treated by *** extract at 250 and 500 mg/kg body weight,cimetidine at 2.5 mg/ kg and verapamil at 10 mg/kg body weight intraperitoneally,*** volume,free and total acidity of gastric secretion were observed and ***:It was found that the extract significantly reduced the volume,free and total acidity of gastric secretion(P<0.01). These reductions were comparable to cimetidine and *** the reduction in the volume and free acidity were more significant in cimetidine and verapamil treated group indicating that cimetidine and verapamil were more ***:The extract of *** can reduced the volume,free and total acidity of gastric secretion,and can be used effectively in the treatment of peptic ulcer.

关键词： Verapamil Cimetidine carbachol Hibiscus rosasinensis Rats

Muscarinic Receptor Agonists Protect Cultured Bovine Trabecular Meshwork Cells against Apoptosis Induced by Dexamethasone

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眼科学报 2004年第1期20卷 42-47页

作者： Yajuan Gu1,Shujun Zeng2,PengXin Qiu2,Yuping Wu2,Dawei Peng2,Guangmei Yan11Department of Pharmacology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510089, China2Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China Department of Pharmacology Zhongshan Medical College Sun Yat-sen University Guangzhou 510089 China.

Purpose:To study whether muscarinic receptor agonists can protect cultured bovine trabecular meshwork cells against apoptosis induced by ***:The third to fifth passags of bovine trabecular meshwork cells were grown to confluence and incubated for 1～14 days in growth media with dexamethasone or pretreatment of pilocarpine or *** cultures were evaluated for apoptosis by phase-contrast microscopy, fluorescence microscopy, DNA laddering and flow cytometric ***:Dexamethasone (0.24～0.96 mmol·L-1) induced apoptosis of trabecular mes-hwork cells in a dose and time-dependent *** 0.48 mmol·L-1 dexametha-sone-treatment, 1.84 mmol·L-1 of pilocarpine or 2.74 mmol·L-1 of carbachol added could significantly reduce apoptotic ***:Muscarinic receptor agonists can protect cultured bovine trabecular meshwork cells against apoptosis induced by dexamethasone. Eye Science 2004;20:42-47.

关键词： apoptosis dexamethasone pilocarpine carbachol trabecular meshwork glaucoma

不饱和脂肪酸对豚鼠胃窦环行肌细胞毒蕈碱电流的影响(英文)

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Acta Pharmacologica Sinica 2003年第3期24卷 93-98页

作者：崔艺峰金海郭慧淑李林禹永春许文燮延边大学医学院生理学教研室延边大学医学院生理学教研室延吉 133000 中国延边大学医院附属医院

目的：研究外源性不饱和脂肪酸对豚鼠胃窦平滑肌细胞素毒蕈碱电流的影响及其作用机制。方法：利用膜片箝技术的全细胞记录法在急性分离的胃窦环行肌细胞上记录毒蕈碱电流。结果：在细胞外灌流液中给予花生四烯酸(arachidonic acid,AA)... 详细信息

目的：研究外源性不饱和脂肪酸对豚鼠胃窦平滑肌细胞素毒蕈碱电流的影响及其作用机制。方法：利用膜片箝技术的全细胞记录法在急性分离的胃窦环行肌细胞上记录毒蕈碱电流。结果：在细胞外灌流液中给予花生四烯酸(arachidonic acid,AA)明显抑制Icch,并具有量效关系：当AA的浓度在1。3和μmo1/L时，分别抑制Icch至46%±8%,23%±5%和3.8%±0.9%;另一种不饱和脂肪酸，亚麻酸(linoleic acid,LA)也抑制Icch，在1,5和10μmo1/L浓度分别抑制Icch至3.8%±0.9%,35%±5%和67%±9%；用H-7(蛋白激酶C抑制剂)100μmo1/L预处理10-15分钟以后，AA分别抑制Icch至5.5%±0.7%和3.0%±1.0%。结论：不饱和脂肪酸直抑制毒蕈碱电流，且抑制程度与不饱和脂肪酸链中的双键数目有关。

关键词： unsaturated fats muscarinic receptors carbachol pyloric antrum guinea pigs

爪蟾顶盖神经元的大微抑制性突触后电流(mIPSCs)依赖从钙储池释放的钙?

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神经科学通报 2005年第2期21卷 101-110页

作者：王红蔡浩然北京大学第一医院中心实验室北京100034

目的　用影响突触前细胞外钙内流及或细胞内钙储池释放钙的措施,研究大、小微抑制性突触后电流(mIPSCs)的一些特性。方法　采用盲法电压钳全细胞记录技术。结果　①无钙液中加或不加EGTA(200mmol/L)灌流时,均可逆地使大mIPSCs较正常含... 详细信息

目的　用影响突触前细胞外钙内流及或细胞内钙储池释放钙的措施,研究大、小微抑制性突触后电流(mIPSCs)的一些特性。方法　采用盲法电压钳全细胞记录技术。结果　①无钙液中加或不加EGTA(200mmol/L)灌流时,均可逆地使大mIPSCs较正常含钙液灌流时明显增多,小mIPSCs的平均频率明显下降;增加细胞外液钙(4mmol/L)浓度,小mIPSCs的频率增加,大mIPSCs变化不明显。Cd2+ (100μmol/L),仅轻度降低小mIPSCs平均频率至对照组的(87. 3±30. 0)% (n=13)。②carbachol(100μmol/L)使小mIPSCs增加至对照组的315. 63%。然而,无钙液中加carbachol,小mIPSCs不增加。含钙液和无钙液中加carbachol均可使大mIPSCs的比例增加。③Thapsigargin(8μmol/L)可使小mIPSCs的平均频率增加至对照组的(132. 1±27. 4)%。④U73122 (40μmol/L)可使小mIPSCs的平均频率降低至对照组的(74. 7±29. 6)%。⑤Procaine(2mmol/L)及咖啡因(10mmol/L)均可降低小mIPSC的平均频率。较高浓度的兰尼定(30 50μmol/L)也降低小mIPSCs的频率。结论　改变灌流液的钙浓度,小mIPSCs的频率受到明显影响。大mIPSCs的出现或增加主要与钙储池释放Ca2+的机制有关。

关键词：抑制性突触后电流顶盖神经元 carbachol 全细胞记录技术平均频率爪蟾 mIPSC Ca^2+ 对照组无钙液细胞内钙细胞外液钙内流突触前电压钳咖啡因兰尼定钙浓度灌流液

Modulation of c-Jun NH<sub>2</sub>-Terminal (JNK) by Cholinergic Autoantibodies from Patients with Sjögren’s Syndrome

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Pharmacology & Pharmacy 2011年第4期2卷 256-265页

作者： Enri Borda Daniela Passafaro Silvia Reina Leonor Sterin-Borda 不详

Background: We wanted to determine (via an immunopharmacological approach) whether the c-Jun NH2 terminal kinase (JNK) cascade is phosphorylated in the submandibular gland by carbachol and cholinergic autoantibodies (IgG) present in the sera of patients with primary Sj?gren’s syndrome (pSS) by interaction and activation of salivary gland muscarinic acetylcholine receptors (mAChRs). Methods: The JNK, PGE2 and NOS assays were measured in rat sub- mandibular gland with pSS IgG and carbachol alone or in the presence of different blocker agents. Results: pSS IgG- activated M3 mAChRs stimulated JNK phosphorylation whereas the activation of M1 mAChRs by carbachol stimulated JNK phosphorylation involving calcium-activated mechanism. The intracellular pathway leading to pSS IgG-induced biological effects on JNK activity involved activation of protein kinase C (PKC), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) enzymes. Also, activation of COX-2 and COX-1 by pSS IgG and carbachol-induced PGE2 generation were involved. Conclusion: These results may contribute to better understanding the modulatory role of JNK enzymes by cholinergic autoantibodies from pSS patients acting on mAChR in rat submandibular gland.

关键词： JNK pSS IgG Autoantibodies PGE2 NOS carbachol Cholinergic Antagonists Enzymatic Inhibitors

Antispasmodic Effects of <i>Salvia officinalis</i>in Isolated Ileum of Rabbit

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Pharmacology & Pharmacy 2019年第5期10卷 223-233页

作者： Wael Al-Aghawani Isam AL Naser Department of Pharmacology and Toxicology Faculty of Pharmacy International University for Science and Technology (IUST) Damascus Syria

Salvia officinalis has been commonly used in Syria and other Middle East countries in popular medicine for many medical purposes. One of the strong claims about its properties is the spasmolytic effect. The aim of this research was to investigate the spasmolytic effect of ethanolic extract of Salvia officinalis in isolated rabbit ileum preparations. Male rabbits were sacrificed and portions of ileum were isolated and mounted in Tyrode’s solution. Afterwards, the experiments were performed according to different groups with different spasmogenic agents: the first group was only with spontaneous contraction, the second was KCl-induced contractions and the third was carbachol-induced contractions. The examined extract was added cumulatively in all previous groups. Verapamil was used as a reference. The results showed profound relaxing effect of S. officinalis extract in mentioned groups, especially in the group of KCl-induced contraction. These findings suggest that the main mechanism of the spasmolytic action is related to the inhibition of calcium entry through cell membrane as well as via internal stores.

关键词： Salvia officinalis Isolated Ileum Verapamil carbachol

Changes of phasic and tonic smooth muscle function of jejunum in type 2 diabetic Goto-Kakizaki rats

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World Journal of Diabetes 2013年第6期4卷 339-348页

作者： Jing-Bo Zhao Peng-Min Chen Hans Gregersen Department of Gastroenterology and Surgery Aalborg University HospitalDK 9000 AalborgDenmark The College of Bioengineering Chongqing University Department of Molecular Biology Institute of Clinical MedicineChina-Japan Friendship Hospital The GIOME Institute GIOME FZERas AlKhaimah 6300United Arab Emirates

AIM:To generate phasic and tonic stress-strain curves for evaluation of intestinal smooth muscle function in type 2 diabetic rats during active and passive ***:Seven diabetic Goto-Kakizaki(GK)male rats,32-wk old(GK group),and 9 age-matched normal Wistar rats(Normal group)were included in the *** segments were distended up to a pressure of10 cm H2O in an organ bath containing 37℃Krebs solution with addition of carbachol(CA).The pressure and outer diameter changes were synchronously *** conditions were obtained using calcium-free Krebs solution containing ethylene glycol tetraacetic acid and *** phasic,tonic and passive circumferential stress and strain were computed from the diameter and pressure data with reference to the zero-stress state *** active phasic and tonic stresses were defined as the total phasic and tonic stresses minus the passive ***:Diabetes increased jejunal mucosa and muscle layer thicknesses compared to the Normal group(mucosa,755.8±63.3 vs 633.1±59.1μm,P0.05;stress,0.25±0.03 vs 0.35±0.06 kPa,P>0.05).The amplitude of total phasic,total tonic,active phasic and active tonic circumferential stresses did not differ for the distensions without CA application between the GK group and the Normal ***,the total phasic and total tonic stresses increased after CA application in the GK group compared those in the Normal *** normalized to muscle layer thickness,the amplitude of active stresses before CA application was lowest in the GK group compared with the Normal *** difference was

关键词： Intestine Diabetes Muscle function Stress-strain curves carbachol Rat