检索结果-南通市图书馆

Plasmid vector based generation of transgenic mesenchymal stem cells with stable expression of reporter gene in caprine

引用

Stem Cell Discovery 2013年第4期3卷 226-239页

作者： Manish Kumar Renu Singh Kuldeep Kumar Pranjali Agarwal Puspendra Saswat Mahapatra Abhisek Kumar Saxena Ajay Kumar Subrata Kumar Bhanja Dhruba Malakar Rajendra Singh Bikas C. Das Sadhan Bag Animal Biotechnology Center National Dairy Research Institute Karnal India Central Avian research Institute Bareilly India Division of Biochemistry Indian Veterinary Research Institute Bareilly India Division of Pathology Indian Veterinary Research Institute Bareilly India Division of Physiology and Climatolxcogy Indian Veterinary Research Institute Bareilly India Division of Surgery Indian Veterinary Research Institute Bareilly India

The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and a novel method of stem cell therapy in the various diseases. Achieving high levels of transgene expression for the longer period of time, without adversely affecting cell viability and differentiation capacity of the cells, is crucial. In the present study, we investigated the efficiency of plasmid vector for the production of transgenic cMSCs and examined any functional change of cells after transfection. To do so first we have collected bone marrows from the adult goats and cultured them for isolation of mesenchymal stem cells (cBM-MSCs). These cells were characterized using MSC specific markers including differentiation into osteocytes and adipocytes. Transfection with plasmid vector did not adversely affect cBM-MSCs morphology, viability or differentiation potential, and transgene expression levels were unaffected beyond passage 12th. The results indicated that we have been able to generate transgenic caprine MSC (tcBM-MSC) and transfection of cBM-MSCs using plasmid vector resulted in very high and stable transfection efficiency. This finding may have considerable significance in improving the efficacy of MSC-based therapies and their tracking in animal model.

关键词： Transgenic MSC caprine Plasmid Vector Characterisation In Vitro Differentiation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Motility and Functional State of the Membrane of caprine Capacitated Spermatozoa under Different Chemical Agents

引用

Open Journal of Veterinary Medicine 2012年第3期2卷 98-103页

作者： A. Soberano Martínez J. Herrera Camacho J. C. Segura Correa Campus Ciencias Biológicas y Agropecuarias-Universidad Autónoma de Yucatán Km 15.5 Carr Mérida México Centro Multidisciplinario de Estudios en Biotecnología Facultad de Medicina Veterinaria y Zootecnia Universidad Michoacana de San Nicolás de Hidalgo Morelia Mexico Instituto de Investigaciones Agropecuarias y Forestales-Universidad Michoacana de San Nicolás de Hidalgo Posta Zootecnia Tarímbaro México

The objective of this study was to test the use of a commercial extender (Triladyl) as a diluent in caprine semen refrigerated at 15℃, using caffeine (CF), heparin (HP), synthetic oviductal fluid (SOF) andtriladyl (TRY) as capacitating chemical agents at different times. Twenty ejaculates of caprine semen were collected using an artificial vagina. The ejaculates were diluted and refrigerated by three days. Evaluated the progressive motility (PM) and the functional state of the sperm plasma membrane trough fluorescent CTC staining, counting 200 spermatic cells (non-capacitated spermatozoa NCS, capacitated CS and reacting spermatozoa REA) of caprine in two capacitating agents: CAF and HEP;one culture medium:SOF and a commercial extender: TRY at 60, 120, 180 and 240 min of incubation, during 24, 48 and 72 hs. PM was high under TRY, and CS was high under the HEP treatment. TRY could be an alternative to capacitate caprine spermatozoa, keeping PM for a longer time than HEP or CAF.

关键词： Spermatozoa Capacitation caprine Chemical Agents

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Determination of caprine serum albumin in milk using bromocresol green dye

引用

Open Journal of Animal Sciences 2013年第3期3卷 261-267页

作者： B. A. Olaniyan X. Zhao A. F. Mustafa Department of Animal Science McGill University Montreal Canada Department of Animal Science McGill University Montreal Canada

The objective of this study was to develop and validate a colorimetric assay to measure caprine serum albumin (CSA) concentrations in milk. The principle of the say was based on the binding of albumin to bromocresol green (BCG) dye at pH 4.0. The absorption of the dye-albumin complex was determined spectrophotometrically at 640 nm. A linear relationship existed between CSA concentrations (0.01 to 8.0 mg/ml) and absorbance values. Intra-assay and interassay coefficients of variation for the proposed assay were 2.6% and 12% respectively. Recovery from milk samples spiked with albumins was 89.15% ± 7.6%. The developed assay was validated using 126 milk samples collected from lactating Saanen goats at various stages of lactation`. caprine serum albumin concentrations in milk ranged between 0.31 and 0.52 mg/ml. The concentrations of CSA were significantly higher

关键词： caprine Milk Serum Albumin Somatic Cell Count

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evaluation of Correlation between Acid Degree Value and Peroxide Value in Lipolysis of Control and Iron Fortified caprine Milk Cheeses during 4 Months Storage

引用

Open Journal of Animal Sciences 2019年第1期9卷 1-11页

作者： Aftab Siddique Young W. Park Georgia Small Ruminant Research and Extension Center Fort Valley State University The University System of Georgia Fort Valley GA USA

Elevation of acid degree values (ADV) and peroxide value (POV) indicates deterioration of nutritional and sensory qualities of dairy products during storage. Iron fortification in bovine milk cheeses in relation to lipolytic parameters and their correlations have been studied, while no such studies have been reported on caprine milk cheeses. The objectives of this study were to evaluate levels of ADV and POV of iron fortified [regular ferrous sulfate (RFS) and large microencapsulated ferrous sulfate (LMFS)] goat milk Cheddar cheeses compared to those of non-fortified control cheese (CC) and their correlations. Three batches of 3 types (CC, RFS and LMFS added) of goat milk cheeses were manufactured and stored at two temperatures (4°C and -18°C) for 0, 2 and 4 months. Iron was supplemented to RFS and LMFS cheeses by 8.23 g and 9.03 g per 9 kg, respectively. ADV and POV were determined using standard methods of examining dairy products (Richardson, 1985) and AOCS (1975) procedures. Results showed that ADV values for CC, RFS and LMFS cheeses for 0 and 4 month storage at 4°C were: 0.67, 0.73, 0.64;1.24, 1.78, 1.58, respectively, indicating significant (P caprine cheeses across storage periods were significant (P < 0.01), while r values of CC cheeses alone were negative. It was concluded that significant correlatio

关键词： caprine Milk Cheese Storage Iron Fortification ADV POV Correlation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Production of Infant Formula Analogs by Membrane Fractionation of caprine Milk: Effect of Temperature Treatment on Membrane Performance

引用

Food and Nutrition Sciences 2011年第10期2卷 1097-1104页

作者： Catherine O. Maduko Young W. Park 不详

A two-step-cascade membrane separation by ultrafiltration was performed on caprine milk prepared under different temperature conditions to eliminate beta-lactoglobulin (β-Lg) from the whey fraction. Effects of temperature treatment and membrane pore size on the elimination of β-Lg and retention of alpha-lactalbumin (α-La) were examined to determine the optimum permeate fraction for production of infant formula analogues from caprine milk. The frozen raw caprine milk, with and without prior pasteurization, showed the best membrane separation performance. The permeates obtained from the 800/30kDa membrane combination showed the optimal results. The infant formula analog produced using the casein and 800/30kDa-permeate fractions of the treated caprine milk had the closest similarity to human milk with respect to the total protein content (1.3g 100g-1), beta-lactoglobulin content (1% - 2%), and casein- α-lactalbumin ratio (0.6 - 0.7). Membrane performance during ultrafiltration of caprine milk was affected by temperature treatment of the milk prior to membrane separation.

关键词： caprine Milk Membrane Fractionation Temperature Effect Infant Formula Analogue

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

In vivo and in vitro development of Tibetan antelope(Pantholops hodgsonii)interspecific cloned embryos

引用

Frontiers of Agricultural Science and Engineering 2014年第1期1卷 21-29页

作者： Guanghua SU Lei CHENG Yu GAO Kun LIU Zhuying WEI Chunling BAI Fengxia YIN Li GAO Guangpeng LI Shorgan BOU The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology Key Laboratory of Herbivore Reproductive Biotechnology and Breeding Ministry of AgricultureInner Mongolia UniversityHohhot 010021China

The Tibetan antelope is endemic to the Tibetan Plateau,China,and is now considered an endangered species.As a possible rescue strategy,the development of embryos constructed by interspecies somatic cell nuclear transfer(iSCNT)was examined.Tibetan antelope fibroblast cells were transferred into enucleated bovine,ovine and caprine oocytes.These cloned embryos were then cultured in vitro or in the oviducts of intermediate animals.Less than 0.5%of the reconstructed antelope-bovine embryos cultured in vitro developed to the blastocyst stage.However,when the cloned antelope-bovine embryos were transferred to caprine oviducts,about 1.6%of the embryos developed to the blastocyst stage.In contrast,only 0.7%of the antelope-ovine embryos developed to the morula stage and none developed to blastocysts in ovine oviducts.The treatment of donor cells and bovine oocytes with trichostatin A did not improve the embryo development even when cultured in the oviducts of ovine and caprine.When the antelope-bovine embryos,constructed from oocytes treated with roscovitine or trichostatin A,were cultured in rabbit oviducts 2.3%and 14.3%developed to blastocysts,respectively.It is concluded that although some success was achieved with the protocols used,interspecies cloning of Tibetan antelope presents difficulties still to be overcome.The mechanisms resulting in the low embryo development need investigation and progress might require a deeper understanding of cellular reprogramming.

关键词： interspecific nuclear transfer bovine ovine caprine oviduct apoptosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Epidemiology and Antibiotic Susceptibility Profile of Methicillin Sensitive Staphylococcus aureus among Livestock and Pet Animals

引用

Veterinary Science Research 2020年第1期2卷 16-23页

作者： Muhammad Aamir Naseer Amjad Islam Aqib Muhammad Shoaib Iqra Muzammil Zeeshan Ahmad Bhutta Iqra Gulzar Department of Clinical Medicine and Surgery University of AgricultureFaisalabad-38000Pakistan Department of Medicine Cholistan University of Veterinary and Animal SciencesBahawalpur63100Pakistan Institute of Microbiology University of AgricultureFaisalabad38000Pakistan Royal(Dick)School of Veterinary Studies The University of EdinburghUnited Kingdom Department of Zoology and Fisheries University of AgricultureFaisalabad-38000Pakistan

Staphylococcus aureus is an important zoonotic pathogen that is responsible for a variety of infectious diseases in humans and animals.The present study was designed to check the prevalence and antimicrobial resistance of MSSA from three different animal origins(bovine,caprine and pet).A total of n=450 samples(150 each source)were collected from bovine,caprine and pets.Collected samples were subjected to S.aureus identification by microbiological examination and confirmed S.aureus isolates were put to oxacillin disk diffusion test to declare them MSSA.The MSSA confirmed isolates were subjected to various antibiotics for susceptibility profiling using Kirby Bauer Disk Diffusion test.The present study found higher prevalence of MSSA from caprine origin(goat 83.33%)as compared to pet(cat 69.33%;dog 65.33%)and bovine origin(buffalo 26.66%;cattle 31.66%).The in-vitro findings of current study revealed oxytetracycline and gentamicin presented 100%efficacy against MSSA of all origins while the vancomycin presented>35%,>40%and>65%resistance against MSSA isolated from bovine,caprine and pet origin respectively.However,ciprofloxacin was equally effective(50%)against MSSA from buffalo and cattle while>80%efficacy was noted against MSSA from cat and dogs.Linezolid and amoxicillin+clavulanic acid were 77.78%and 66.67%sensitive to MSSA isolates from caprine milk.The present study found higher prevalence of MSSA from bovine,caprine and pet isolates with diversified pattern of susceptibility of different antibiotics from all sources.

关键词： S.aureus MSSA Pet Bovine caprine Antibiotic susceptibility

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Determination of Selected Heavy Metals in the Organs of Some Animals Reared in the Gold-Mining Areas of Zamfara State, Nigeria

引用

Journal of Agricultural Chemistry and Environment 2018年第4期7卷 188-202页

作者： U. A. Birnin-Yauri M. K. Musa S. M. Alhaji Department of Pure and Applied Chemistry Usmanu Danfodiyo University Sokoto Nigeria Department of Chemistry Sokoto State University Sokoto Nigeria

The study was conducted to determine the concentrations of heavy metals (Cu, Zn, Co, Mn, Fe, Cr, Cd and Pb) in the liver, kidney and flesh of Cattle, Sheep and Goats from Anka and Bukkuyum Local Government areas of Zamfara State Nigeria. The levels of heavy metals in the liver, kidney and flesh of beef, mutton and caprine ranged from 56 to 549 μg·g-1 for Zn;109 to 576 μg·g-1 for Fe;0.0 to 4.0 μg·g-1 for Pb;2.52 to 13.25 μg·g-1 for Mn;0.00 to 19.64 μg·g-1 for Cr;0.00 to 0.25 μg·g-1 for Cd;0.00 to 2.71 μg·g-1 for Co and 3.59 to 538 μg·g-1 for Cu. The concentration of Cr, Pb and Mn in the kidneys and livers of the samples were observed to be higher than the maximum allowable level. Similarly, the exception of Cr in the liver of sheep and goat from Anka and liver of sheep from Bukkuyum, Pb in some liver and kidney samples and Mn in some liver, kidney samples and flesh of cattle from Anka were found to be polluted and hence unsafe for consumption.

关键词： Heavy Metals caprine Mutton Beef Environment Cattle Sheep Goats

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ovine Progressive Pneumonia Virus Is Transmitted More Effectively via Aerosol Nebulization than Oral Administration

引用

Open Journal of Veterinary Medicine 2012年第3期2卷 113-119页

作者： Lynn M. Herrmann-Hoesing Stephen N. White Liam E. Broughton-Neiswanger Wendell C. Johnson Susan M. Noh David A. Schneider Hong Li Naomi S. Taus James Reynolds Thomas Truscott Rohana P. Dassanayake Donald P. Knowles Animal Disease Research Unit U.S. Department of Agriculture-Agricultural Research Service Washington State University Pullman USA Department of Veterinary Microbiology and Pathology Washington State University Pullman USA

A new method of experimental infection of ovine progressive pneumonia virus (OPPV), aerosol nebulization (Nb), was compared to intravenous (IV) and oral (PO) methods of experimental infection. Seven month old lambs were given 3.5 × 107 TCID50 of Dubois OPPV LMH19 isolate using IV, PO, or Nb methods and were monitored for infection using cELISA and OPPV quantitative (q) PCR for 35 weeks. Four out of four sheep in the IV group, six out of six sheep in the Nb group, but only two out of six sheep in the PO group became infected by OPPV;whereas the uninoculated controls (n = 2) and a sentinel control (n = 1) remained uninfected during the course of the study. The time to a cELISA or OPPV qPCR positive result in the Nb group was quicker and statistically different from the time to a cELISA or OPPV qPCR positive result in the PO group (cELISA P value = 0.0021 and OPPV qPCR P value = 0.0007). When the Nb and IV groups were compared, sheep became cELISA and OPPV qPCR positive at similar times (cELISA P value = 0.6 and OPPV qPCR P value = 0.1). In addition, sheep became OPPV qPCR positive prior to cELISA in both the IV and Nb groups (IV P value = 0.027 and Nb P value = 0.007). Aerosol nebulization is a more natural experimental method of transmitting OPPV and may be valuable for testing potential vaccines or specific host genetics.

关键词： Ovine Progressive Pneumonia Virus = OPPV Visna/Maedi Virus = VMV Small Ruminant Lentivirus = SRLV caprine Arthritis-Encephalitis Virus = CAEV Transmission

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：