检索结果-南通市图书馆

Protective efficacy of vaccination with NcMIC3 and NcMIC8 against Neospora caninum infection in mice

Frontiers of Agricultural Science and Engineering 2019年第2期6卷 188-196页

作者： Taotao ZHANG Xiao ZHANG Qun LIU Jianhai XU Jing LIU National Animal Protozoa Laboratory College of Veterinary Medicine China Agricultural University

Microneme proteins (MICs) are important for Apicomplexan parasite invasion due to their adhesion to host cells. Several studies have indicated that Neospora caninum MIC3 and MIC8 are important adhesion factors and potential vaccine candidates against neosporosis. In this study, we evaluated the protective efficacy of recombinant proteins and DNA vaccines of NcMIC3 and NcMIC8. BALB/c mice were immunized with rNcMIC3, rNcMIC8, pcDNA3.1-NcMIC3 and pcDNA3.1-NcMIC8 respectively, and challenged with N. caninum tachyzoites. The immune responses were evaluated through cytokine, antibody measurements and the parasite burden in the mice brain tissues. Serological analysis showed that recombinant protein vaccines induced higher levels of immunoglobulin G (IgG) than other groups. The percentage of IgGl and IgG2a in the recombinant protein groups was higher than the other groups, and with a predominance of IgGl over IgG2a, suggesting that recombinant protein vaccines elicited a Th2-type immune response, while DNA vaccines mainly produce a Th 1-type immune response. In addition, mice immunized with rNcMIC3 and rNcMIC8 a had lower parasite burden in brain tissue compared with the other groups. These results demonstrate that rNcMIC3 and rNcMIC8 could induce humoral and Th2-type immune response, leading to a considerable level of resistance against neosporosis.

关键词： NcMIC3 NcMIC8 Neospora caninum vaccination

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prevalence of Antibodies to <i>Toxoplasma gondii</i>and <i>Neospora caninum</i>in Pigs in Grenada, West Indies

引用

Open Journal of Veterinary Medicine 2015年第6期5卷 138-141页

作者： Ravindra Nath Sharma Keshaw Tiwari Alfred Chikweto Claude DeAllie Muhammad Iqbal Bhaiyat Department of Pathobiology School of Veterinary Medicine St. George’s University St. George Grenada Veterinary and Livestock Division Ministry of Agriculture Land Fisheries and Environment Grenada

The aim of the study was to estimate the prevalence of antibodies to Toxoplasma gondii (T. gondii) and Neospora caninum (N. caninum) in pigs in Grenada, West Indies. T. gondii is a serious zoonosis affecting the unborn fetus and immunocompromized individuals. N. caninum is a similar coccidian parasite, which is not zoonotic, but is the cause of abortion and neonatal mortality in livestock similar to T. gondii. An earlier study conducted in Grenada and using a modified agglutination test (MAT) revealed seropositivity to T. gondii in pigs. No information is available on N. caninum infection of pigs in the Caribbean islands including Grenada. Serum samples from 185 pigs in Grenada, West Indies were tested for antibodies to T. gondii and N. caninum using an indirect enzyme linked immunosorbent assay (ELISA). Antibodies to T. gondii were found in 24.3% of pigs (95% confidence interval (CI): 18.12% to 30.48%) as all the tested pigs were negative for antibodies to N. caninum. Although, seroprevalence for T. gondii was higher in females (25.75%) than in males (20.70%), this result was statistically insignificant (p = 0.57). The results were similar to a previous study in Grenada confirming the continuity of infection in pigs. Human Toxoplasmosis is transmitted mainly through ingestion of tissue cysts in contaminated raw or undercooked meat or sporulated oocysts in soil, water or vegetables. Education of farmers and the Grenadian community on epidemiology of these parasites is warranted to prevent infection in pigs and in humans. This is the first report on the seroprevalence of N. caninum in pigs in the Caribbean region.

关键词： Toxoplasma gondii Neospora caninum Pigs MAT ELISA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

用重组蛋白NcSAG1t作为ELISA诊断抗原进行改良绒山羊Neospora caninum的血清学诊断

引用

中国畜牧兽医 2007年第2期34卷 109-111页

作者：陆艳王戈平马利青青海省畜牧兽医科学院西宁810016

Ncaninum的表面抗原1(NcSAG1)在建立新孢子虫病的诊断上是一个重要的诊断抗原。为了建立有效的诊断方法,基因编码的截头(NcSAG1t)缺失一个信使缩氨酸和C-末端的疏水性区域的基因,象谷胱甘肽转移酶(GST)的融合蛋白一样被克隆和表达在esch... 详细信息

Ncaninum的表面抗原1(NcSAG1)在建立新孢子虫病的诊断上是一个重要的诊断抗原。为了建立有效的诊断方法,基因编码的截头(NcSAG1t)缺失一个信使缩氨酸和C-末端的疏水性区域的基因,象谷胱甘肽转移酶(GST)的融合蛋白一样被克隆和表达在escherichiacoli。纯化的GST-NcSAG1t作为ELISA诊断抗原被用在改良绒山羊N caninum抗体的检测中。经过对207份改良绒山羊血清样品进行检测,检出Ncaninum抗体阳性16份,阳性率为7.73%。结果初步说明青海省的改良绒山羊羊群中存在Ncaninum。

关键词： N caninum NcSAG1t ELISA 改良绒山羊抗体

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Serological survey on some pathogens in wild brown hares(Lepus europaeus) in Central Italy

引用

Asian Pacific Journal of Tropical Medicine 2016年第5期9卷 452-455页

作者： Valentina Virginia Ebani Alessandro Poli Guido Rocchigiani Fabrizio Bertelloni Simona Nardoni Roberto Amerigo Papini Francesca Mancianti Department of Veterinary Sciences University of Pisa

Objective: To determine the exposure of wild brown hares [Lepus europaeus(L. europaeus), pallas] to Anaplasma phagocytophilum(A. phagocytophilum), Borrelia burgdorferi(B. burgdorferi) sensu lato, Encephalitozoon cuniculi(E. cuniculi), Leishmania sp., Neospora caninum(N. caninum) and Toxoplasma gondii(T. gondii). Methods: Two hundred twentytwo blood serum samples of wild brown hares captured in protected areas of the province of Pisa(Central Italy) were tested to detect antibodies against the reported pathogens. Results: Thirty one(14.0%) animals resulted positive for at least one tested agent, with antibody titres ranging from 1:20 to 1:320. In particular, 13(5.8%) samples were positive to B. burgdorferis.l., 11(4.9%) to N. caninum, 3(1.3%) to T. gondii, 2(0.9%) to A. phagocytophilum and 2(0.9%) to Leishmania sp. No samples scored positive to E. cuniculi. Four animals(14.8%) resulted coinfected with 2 different pathogens. Conclusion: The obtained results showed that B. burgdorferi s.l. N. caninum, T. gondii, A. phagocytophilum and Leishmania sp circulate in wild brown hares in Central Italy, suggesting a possible role of L. europaeus as reservoir of these pathogens. The obtained results showed that autochthonous wild brown hares living in Central Italy have been exposed to several pathogens circulating in this area, suggesting a possible role of L. europaeus as reservoir.

关键词： Anaplasma phagocytophilum Borrelia burgdorferi s.l. Brown hare(Lepus europaeus) Leishmania sp. Neospora caninum Toxoplasma gondii Serology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

ELISA法筛选抗新孢子虫中药的研究(英文)

引用

Agricultural Science & Technology 2008年第6期9卷 102-103页

作者：孔琳鞠玉琳延边大学农学院吉林龙井133400

[Objective] The study was to screen the Chinese medicines with good resistance to Neospora caninum. [Method] Healthy Kunming mice pretreated with methylprednisolone for the purpose of immunity decrease were randomly divided into 15 groups, and each mouse was intraperitoneally inoculated with 1.0 ×104 N. caninum. Four hours later, the mice were gavaged with various Chinese medicines. Seven days post-administration, eyeball blood sampling was conducted and the serum was used for antibody level detection with ELISA method. [Result] Four among the 15 Chinese medicines showed lower antibody positive rates. [Conclusion] Scutellaria baicalensis, Stemona sessilifolia, Gastrodia elata and Coptis chinensis could enhance the immunity level of mice infected by N. caninum.

关键词： ELISA method Chinese medicine Neospora caninum Antibody level

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：