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A High-Throughput Method for Screening Arabidopsis Mutants with Disordered Abiotic Stress-Induced calcium signal

Journal of Genetics and Genomics 2012年第5期39卷 225-235页

作者： Zhen Pan Yang Zhao Yuan Zheng Juntao Liu Xiangning Jiang Yan Guo College of Life Sciences and Bioteclmology Beijing Forestry University Beijing 100083 China State Key Latboratory of Plant Physiology and Biochemistry. College of Biological Sciences. China Agricultural University Beijing 100193 China

It is established that different stresses cause signal-specific changes in cellular Ca2 ~ level, which function as messengers in modulating diverse physiological processes. These calcium signals are important for stress adaptation. Though numbers of downstream components of calcium signal cascades have been identified, upstream events in calcium signal remain elusive, specifically components required l＇~~r calcium signal generation due to the lack of high-throughput genetic assay. Here, we report the development of an easy and efficient method in a forward genetic screen for Ca2＋ signals-deficient mutants in Arahidopsis thaliana. Using this method, 121 mutants with disordered NaCI- and H=O2-induced Ca2＋ signals are isolated.

关键词： Arabidopsis" Mutant screen method calcium signal Abiotic stress

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SCAB1 coordinates sequential Ca^(2+) and ABA signals during osmotic stress induced stomatal closure in Arabidopsis

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Science China(Life Sciences) 2024年第1期67卷 1-18页

作者： Tianren Zhang Li Bai Yan Guo State Key Laboratory of Plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBeijing 100193China

Hyperosmotic stress caused by drought is a detrimental threat to plant growth and agricultural productivity due to limited water *** are gateways of transpiration and gas exchange,the swift adjustment of stomatal aperture has a strong influence on plant drought *** intensive investigations of stomatal closure during drought stress in past decades,little is known about how sequential signals are integrated during complete ***,we discovered that the rapid Ca^(2+) signaling and subsequent abscisic acid(ABA)signaling contribute to the kinetics of both F-actin reorganizations and stomatal closure in Arabidopsis thaliana,while STOMATAL CLOSURE-RELATED ACTIN BINDING PROTEIN1(SCAB1)is the molecular switch for this entire *** the early stage of osmotic shock responses,swift elevated calcium signaling promotes SCAB1 phosphorylation through calcium sensors calcium DEPENDENT PROTEIN KINASE3(CPK3)and *** phosphorylation restrained the microfilament binding affinity of SCAB1,which bring about the Factin disassembly and stomatal closure *** the osmotic stress signal continued,both the kinase activity of CPK3 and the phosphorylation level of SCAB1 attenuated *** further found that ABA signaling is indispensable for these attenuations,which presumably contributed to the actin filament reassembly process as well as completion of stomatal ***,the dynamic changes of SCAB1 phosphorylation status are crucial for the kinetics of stomatal *** together,our results support a model in which SCAB1 works as a molecular switch,and directs the microfilament rearrangement through integrating the sequentially generated Ca^(2+) and ABA signals during osmotic stress induced stomatal closure.

关键词： calcium signal ABA stomatal movement microfilaments kinase

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Downregulation of Kcnt1 by high-potassium treatment during calcium/PI3K signal pathway in primary culture of cerebral cortical neurons

Downregulation of Kcnt1 by high-potassium treatment during c...

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中国神经科学学会第十二届全国学术会议

作者： Kai Gao Yuwu Jiang Department of Pediatrics Peking University First Hospital

Objective Potassium channels are the most numerous and widespread ion channels in the human body and are associated with a series of neurological diseases including epilepsy. KCNT1, which is also called SLO2.2, encodes a sodium-activated potassium channel which is the largest known potassium channel subunit. Recently, a series of studies have identified KCNT1 to be a novel driver of pathogenesis in some epileptic syndromes, such as malignant migrating partial seizures of infancy（MMPSI） and autosomal dominant nocturnal frontal lobe epilepsy（ANDFLE）. Methods In this study, we tested the expression of KCNT1 in high potassium model of epilepsy in in primary culture of mouse cerebral cortical neurons. Results（1） We found that Kcnt1 expression was downregulated after high-potassium treatment.（2） We clarified that the downregulation of Kcnt1 was regulated by high-potassium induced calcium/PI3 K signal pathway. Conclusion Kcnt1 expression is downregulated by high-potassium treatment during calcium/PI3 K signal pathway in primary culture of cerebral cortical neurons

关键词： KCNT1 epilepsy calcium signal PI3K

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The Memory Orchestra:Contribution of Astrocytes

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Neuroscience Bulletin 2023年第3期39卷 409-424页

作者： Yi-Hua Chen Shi-Yang Jin Jian-Ming Yang Tian-Ming Gao State Key Laboratory of Organ Failure Research Key Laboratory of Mental Health of the Ministry of EducationGuangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired IntelligenceGuangdong Province Key Laboratory of Psychiatric DisordersDepartment of NeurobiologySchool of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China

For decades,memory research has centered on the role of neurons,which do not function in ***,astrocytes play important roles in regulating neuronal recruitment and function at the local and network levels,forming the basis for information processing as well as memory formation and *** this review,we discuss the role of astrocytes in memory functions and their cellular underpinnings at multiple time *** summarize important breakthroughs and controversies in the field as well as potential avenues to further illuminate the role of astrocytes in memory processes.

关键词： Astrocytes Learning and memory GPCR calcium signal Plasticity

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Actin filaments,calcium signals and stress responses

Actin filaments,calcium signals and stress responses

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2011全国植物生物学研讨会

作者： Yang Zhao,Zhen Pan,Yan Guo~* State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing 100193,China

Microfilament dynamics plays a critical role in regulating stomatal movement. However,the molecular mechanism underlying this process is not well understood. We report here the identification and characterization of an Arabidopsis actin-binding protein SCAB1,named for the Stomatal Closing related Actin Binding protein l. Plants lacking SCAB1 are hypersensitive to drought stress and exhibit reduced ABA-, HO●,and CaCl-regulated stomatal *** vitro and in vivo analyses reveal that SCAB 1 binds to,stabilizes and bundles actin *** stomatal closure, actin filament configuration undergoes a change from a radial orientation in opened stomata to a longitudinal orientation in closed *** switch of the two

关键词： actin filament calcium signal abiotic stress

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Functions and mechanisms of the CBL-CIPK signaling system in plant response to abiotic stress

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Progress in Natural Science:Materials International 2009年第6期19卷 667-676页

作者： Ruifen Li a,1, Junwen Zhang a,b,1, Jianhua Wei a, Hongzhi Wanga, Yanzhen Wanga, Rongcai Ma a, a Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China b Life Science College, Capital Normal University, Beijing 100037, China Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Sciences Beijing 100097 China Life Science College Capital Normal University Beijing 100037 China

To cope with environmental stimuli, plants have evolved precise regulatory mechanisms to perceive, transduce and respond to abiotic stresses that can negatively affect growth and development. The CBL-CIPK signaling system is a newly emerging plant-specific and Ca2+-dependent network mediating abiotic stress tolerance. CBLs may sense a Ca2+ signature triggered by abiotic stresses, and have specific interactions with novel CIPK-type kinases after binding Ca2+. The CBL/CIPK complexes may post-translationally phosphory-late downstream target proteins to regulate abiotic stress tolerance in a cell or tissue-specific manner. In some cases transcription factors are induced to activate stress-responsive genes that control adaptation reactions. The CBL-CIPK signaling system exhibits specificity, diversity and complexity. Meanwhile, cross talk also exists in the CBL-CIPK signaling. To date, significant progress has been made in the role of the CBL-CIPK signaling system in responding to salt, low K+ and to high pH, which will provide a fast and efficient method of molecular design breeding combined with the CBL/CIPK engineering of crop plants, for enhanced tolerance to abiotic stresses. However, more CBL/CIPK components remain to be identified, particularly from specific plants that grow in conditions with abiotic stress, and the specificity of their abiotic stress signaling will need to be dissected.

关键词： calcium signal CBL CIPK signal transduction Abiotic stress

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