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Novel caga ELISA exhibits enhanced sensitivity of Helicobacter pylori caga antibody

World Journal of Gastroenterology 2017年第1期23卷 48-59页

作者： Yuichi Matsuo Yasutoshi Kido Junko Akada Seiji Shiota Tran Thanh Binh Tran Thi Huyen Trang Ho DQ Dung Pham Huu Tung Tran Dinh Tri Ngo P Minh Thuan Le Quang Tam Bui Chi Nam Vu Van Khien Yoshio Yamaoka Department of Environmental and Preventive Medicine Oita University Faculty of Medicine Department of General Medicine Oita University Faculty of Medicine Department of Endoscopy Cho Ray Hospital Department of Molecular Biology 108 Military Central Hospital Department of Endoscopy Daklak Hospital Department of Endoscopy Lao Cai Hospital Department of Hepatogastroentrology 108 Military Center Hospital Department of Medicine-Gastroenterology Baylor College of Medicine and Michael E.De Bakey Veterans Affairs Medical Center

AIMTo develop a novel Helicobacter pylori (H. pylori) caga antibody enzyme-linked immunosorbent assay (ELISA) suitable for detecting serum anti-caga antibodies with high *** East Asian-type caga protein was purified and immobilized for ELISA. Serum samples from 217 Vietnamese individuals (110 H. pylori-infected and 107 uninfected individuals) were applied. Conventional ELISA from Western-type caga and our East Asian-type caga ELISA were evaluated by comparing 38 subjects with the Western-type genotype and 72 subjects with the East Asian-type caga genotype. Histological scores of the gastric mucosa were determined using the updated Sydney System to examine the relationship with anti-caga antibody *** 70-100 kDa fragments were immobilized on the ELISA plate. In ROC analysis, the area under the curve of our East Asian-type caga ELISA was comparable to that of conventional caga ELISA. The sensitivity of the two ELISAs differed depending on the caga genotype. The sensitivity of East Asian-type caga ELISA was higher for subjects infected with East Asian-type caga H. pylori (P caga ELISA tended to be higher for subjects infected with Western caga H. pylori (P = 0.056). The titer of anti-caga antibody tended to correlate with monocyte infiltration scores (r = 0.25, P = 0.058) and was inversely correlated with H. pylori density (r = -0.26, P = 0.043).CONCLUSIONThe novel ELISA is useful to detect anti-caga antibodies in East Asian countries, and the titer may be a marker for predicting chronic gastritis.

关键词： Helicobacter pylori caga genotype Anti-caga antibody Enzyme-linked immunosorbent assay Inflammation Gastritis

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