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Functional assessment of cadherin as a shared mechanism for cross/dual resistance to Cry1Ac and Cry2Ab in Helicoverpa zea

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Journal of Integrative Agriculture 2024年第5期23卷 1604-1617页

作者： Jizhen Wei Min Zhang Pin Li Zhongyuan Deng Xinming Yin Shiheng An Xianchun Li State Key Laboratory of Wheat and Maize Crop Science/College of Plant Protection Henan Agricultural UniversityZhengzhou 450046China Department of Entomology and BIO5 Institute University of ArizonaTucsonAZ 85721USA School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001China

Helicoverpa zea is a major target pest of pyramided transgenic crops expressing Cry1,Cry2 and/or Vip3Aa proteins from Bacillus thuringiensis(Bt)in the United States.Laboratory-selected Cry1Ac/Cry2Ab cross resistance and fieldevolved practical dual resistance of H.zea to these two toxins have been widely reported.Whether the widespread Cry1Ac/Cy2Ab dual resistance of H.zea has resulted from the selection of one shared or two independent resistance mechanisms by pyramided Bt crops remains unclear.cadherin is a well-confirmed receptor of Cry1Ac and a suggested receptor of Cry2Ab in at least three Lepidopteran species.To test whether cadherin may serve as one shared mechanism for the cross and dual resistance of H.zea to Cry1Ac and Cry2Ab,we cloned H.zea cadherin(Hzcadherin)cDNA and studied its functional roles in the mode of action of Cry1Ac and Cry2Ab by gain-and lossof-function analyses.Heterologous expression of Hzcadherin in H.zea midgut,H.zea fat body and Sf9 cells made all three of these cell lines more susceptible to activated Cry1Ac but not activated Cry2Ab,whereas silencing Hzcadherin of H.zea midgut and fat body cells significantly reduced the susceptibility to Cry1Ac but not Cry2Ab.Likewise,suppressing Hzcadherin with siRNA made H.zea larvae resistant to Cry1Ac.These results clearly demonstrate that Hzcadherin is not a receptor for Cry2Ab,and thus it is unlikely to serve as one shared mechanism for the cross and dual resistance of H.zea to Cry1Ac and Cry2Ab.

关键词： Bt crops cadherin Cry toxin receptor resistance mechanism

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Genome-wide identification and comparative analysis of Cry toxin receptor families in 7 insect species with a focus on Spodoptera litura

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Insect Science 2022年第3期29卷 783-800页

作者： Qilin Li Mengge Li Mengyao Zhu Jielai Zhong Liang Wen Jie Zhang Ruonan Zhang Qiang Gao Xiao-Qiang Yu Yuzhen Lu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhou510631China College of Biology Hunan UniversityChangsha410082China

cadherin,aminopeptidase N(APN)and alkaline phosphatase(ALP)have been characterized as Cry receptors.In this study,comparative genomic analysis of the 3 receptor families was performed in 7 insects.ALPs and APNs are divided into three and eight clades in phylogenetic trees,respectively.ALPs in clade 3 and APNs in clade 1 contain multiple paralogs within each species and most paralogs are located closely in chromosomes.Drosophila melanogaster has expanded APNs in clade 5 and were lowly expressed in midgut.cadherins are divided into 16 clades;they may diverge before holometabolous insect speciation except for BtR and Cad89D-like clades.Eight insects from different orders containing BtR orthologs are sensitive to Cryl A or Cry3A,while five species without BtR are insensitive to both toxins.Most APNs in clade 1,several ALPs in clade 3,BtR and Cad89D-like genes were highly or moderately expressed in larval midgut of Spodoptera litura and the other six species,and several members in these clades have been identi-•fied as Cry receptors.Expressions of putative 5.litura Cry receptors in the midgut after exposing to Bt toxins were also analyzed.

关键词： alkaline phosphatase aminopeptidase N Bacillus thuringiensis cadherin Cry toxin

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cadherin-based biomaterials: Inducing stem cell fate towards tissue construction and therapeutics

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Progress in Natural Science:Materials International 2020年第5期30卷 597-608页

作者： Yan Zhang Zheng Qin Zhanyuan Qu Min Ge Jun Yang The Key Laboratory of Bioactive Materials Ministry of Education College of Life Science Nankai University State Key Laboratory of Medicinal Chemical Biology Nankai University

The precise regulation of cell fate by biomaterials is one of the core focuses in tissue engineering and regenerative therapeutics. Cell fate is controlled by the niche signaling cues, including growth factors/morphogens, direct cell-cell contact, and extracellular matrix components. Here, we discuss how to bio-mimic cell-cell interaction by tailoring the interface of biomaterials to design effective scaffolds. cadherins, as a key cell membrane protein family mediating the intercellular adherens junction, have attracted extensive attention in the functional modification of biomaterials. Therefore, cadherin-based biomaterials, their ability to modulate stem cell fate, and their applications in regenerative medicine are reviewed. Furthermore, we provide an outlook for exploiting the bio-mimic biomaterials in advanced cell therapeutics.

关键词： cadherin Mimic cell-cell interactions Biomaterials Stem cell Tissue construction

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Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis

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Insect Science 2010年第5期17卷 393-399页

作者： Hong-Kun Yu Hao Ghen Yong-Jun Zhang Kong-Ming Wu Ge-Mei Liang Ze-Wen Liu Yu-Yuan Guo State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects Ministry of Agriculture College of Plant Protection Nanjing Agricultural University Nanjing China

The rice stem borer, Chilo suppressalis Walker is one of the most important insect pests on rice in Asia, north Africa and southern Europe. Transgenic Bt rice has been developed in the laboratory with good resistance to this pest and other Lepidopteran insects, which will provide a possible alternative tool for this pest control. The full-length cDNAs encoding an aminopeptidase N （CsAPN） and a cadherin （CsCad） were cloned from C. suppressalis. CsAPN showed common features of, and high identities to, other insect APNs in its deduced amino acid sequence. Although a full-length cDNA encoding cadherin-like protein has been reported in GenBank, the newly isolated cadherin here （CsCad） showed some differences in its amino acid sequence, especially at the 7th cadherin repeat region （CR7）, which indicated the newly isolated CsCad might be another allele. CsAPN and CsCad were successfully expressed in insect Tn cells, and the blot analysis showed these two proteins could bind Bt toxin CrylAb. The results will provide valuable information for the studies of toxin mode of action and the possible toxin resistance mechanisms in this pest.

关键词： aminopeptidase N cadherin Chilo suppressalis heterologous expression,Tn cells

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Cell adhesion in renal tubular epithelial cells:Biochemistry,biophysics or both

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BIOCELL 2022年第4期46卷 937-940页

作者： CLAIRE ELIZABETH HILLS ELEFTHERIOS SIAMANTOURAS PAUL EDWARD SQUIRES Diabetes Metabolism&Inflammation GroupSchool of Life SciencesJoseph Banks LaboratoriesUniversity of LincolnLincolnLN67DLUK

Changes in cell-cell and cell-substrate adhesion markers are increasingly used to characterize disease onset and progression.However,these relationships depend on both the biochemical and molecular association between cells and between cells and their extracellular matrix,as well as the biophysical and mechanical properties orchestrated by cytoskeletal,membrane and matrix components.To fully appreciate the role of cell adhesion when determining normal physiology and the impact of disease on cellular function,it is important to consider both biochemical and biophysical attributes of the system being investigated.In this short viewpoint we reflect on our experiences assessing cell-cell and/or cell-matrix interactions in renal tubular epithelial cells.

关键词： Cell-adhesion cadherin Cytoskeleton AFM Kidney Epithelium

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Relationship between expressions of E-cadherin and alpha-catenin and biological behaviors of human pancreatic cancer

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Hepatobiliary & Pancreatic Diseases International 2003年第2期2卷 471-477页

作者： Yu-Jun Li Yun-Xiao Meng Xiang-Rui Ji the Department of Pathology. Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China Department of Pathology Affiliated Hospital of Medical College Qingdao University Qingdao 266003 China. yujun-li66@***

OBJECTIVES: To investigate the expressions of E-eadherin and alpha-catenin in pancreatic carcinoma and their relationship with biological behaviors, and clarify the mechanism of invasion and metastasis of pancreatic cancer. METHODS: The expressions of E-eadherin and alpha-catenin was examined in 47 patients with infiltrative ductal adenocarcinoma of the pancreas and 12 specimens of normal pancreatic tissues by immunohistochemical technique (PicTure^(TM) two-step method). Proliferation cell nuclear antigen (PCNA) was tested as an index of the proliferation degree of pancreatic cancer cells. RESULTS: The immunoreactivity of E-cadherin and alpha-catenin was expressed by normal ductal and acinar cells with strong membranous staining at the intercellular border in 12 specimens of normal pancreatic tissues. The abnormal rate of E-cadherin expression in pancreatic cancer was 53.2% (25/47), and it was significantly related to differentiation, high proliferation degree and lymph node and liver metastases (Pcadherin and alpha-catenin, tumor size, invasion, and 1-year survival rate of patients ( P>0.05, all). There was a positive relationship between the expressions of E-cadherin and alpha-catenin in the 47 patients with pancreatic cancer (Pcadherin and alpha-catenin genes and abnormal expression of proteins, which significantly correlate with tumorigenesis, proliferation, differentiation, and lymph node or liver metastasis of pancreatic cancer.

关键词： pancreatic neoplasms cadherin catenin metastases prognosis immunohistochemistry

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Unfractionated heparin attenuates endothelial barrier dysfunction via the phosphatidylinositol-3 kinase/serine/threonine kinase/nuclear factor kappa-B pathway

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Chinese Medical Journal 2020年第15期133卷 1815-1823页

作者： Sheng-Tian Mu Jie Tang Jian-Qi Ma Yu Zhong Han-Zhe Liu Xiao-Chun Ma Zhen Zheng Department of Intensive Care Unit Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital and InstituteShenyangLiaoning 110042China Department of Intensive Care Unit The First Affiliated Hospital of China Medical UniversityShenyangLiaoning 110001China

Background::Vascular endothelial dysfunction is considered a key pathophysiologic process for the development of acute lung injury.In this study,we aimed at investigating the effects of unfractionated heparin(UFH)on the lipopolysaccharide(LPS)-induced changes of vascular endothelial-cadherin(VE-cadherin)and the potential underlying mechanisms.Methods::Male C57BL/6 J mice were randomized into three groups:vehicle,LPS,and LPS+UFH groups.Intraperitoneal injection of 30 mg/kg LPS was used to induce sepsis.Mice in the LPS+UFH group received subcutaneous injection of 8 U UFH 0.5 h before LPS injection.The lung tissue of the mice was collected for assessing lung injury by measuring the lung wet/dry(W/D)weight ratio and observing histological changes.Human pulmonary microvascular endothelial cells(HPMECs)were cultured and used to analyze the effects of UFH on LPS-or tumor necrosis factor-alpha(TNF-α)-induced vascular hyperpermeability,membrane expression of VE-cadherin,p120-catenin,and phosphorylated myosin light chain(p-MLC),and F-actin remodeling,and on the LPS-induced activation of the phosphatidylinositol-3 kinase(PI3K)/serine/threonine kinase(Akt)/nuclear factor kappa-B(NF-κB)signaling pathway.Results::In vivo,UFH pretreatment significantly attenuated LPS-induced pulmonary histopathological changes(neutrophil infiltration and erythrocyte effusion,alveolus pulmonis collapse,and thicker septum),decreased the lung W/D,and increased protein concentration(LPS vs.LPS+UFH:0.57±0.04 vs.0.32±0.04 mg/mL,P=0.0092),total cell count(LPS vs.LPS+UFH:9.57±1.23 vs.3.65±0.78×105/mL,P=0.0155),polymorphonuclear neutrophil percentage(LPS vs.LPS+UFH:88.05%±2.88%vs.22.20%±3.92%,P=0.0002),and TNF-α(460.33±23.48 vs.189.33±14.19 pg/mL,P=0.0006)in the bronchoalveolar lavage fluid.In vitro,UFH pre-treatment prevented the LPS-induced decrease in the membrane expression of VE-cadherin(LPS vs.LPS+UFH:0.368±0.044 vs.0.716±0.064,P=0.0114)and p120-catenin(LPS vs.LPS+UFH:0.208±0.018 vs.0.924±0.092,P=0.0016),and the LPS-induced increase in the express

关键词： Acute lung injury cadherin Capillary permeability Heparin

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Association between cadherin-17 expression and pathological characteristics of gastric cancer:A meta-analysis

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World Journal of Gastroenterology 2015年第12期21卷 3694-3705页

作者： Zi-Wen Long Meng-Long Zhou Jing-Wei Fu Xian-Qun Chu Ya-Nong Wang Department of Gastric Cancer and Soft-tissue Sarcoma Surgery Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University

AIM: To construct a meta-analysis in order to examine the relationship between cadherin-17(CDH17) andgastric cancer(GC). METHODS: Related articles were selected by searching the following English or Chinese electronic databases: CINAHL, MEDLINE, Science Citation Index, the Chinese Journal Full-Text, and the Weipu Journal. NewcastleOttawa Scale(NOS) criteria were used to ensure consistency in reviewing and reporting results. Statistical analyses were conducted with Version 12.0 STATA statistical software.RESULTS: Ultimately, 11 articles, with a total of 2,120 GC patients, were found to be eligible for study inclusion. In comparisons of GC patients by TNM stage(Ⅲ-Ⅳ vs ?Ⅰ-Ⅱ: OR = 2.35, 95%CI: 1.15-4.825, P = 0.019), histologic grade(3-4 vs 1-2: OR = 3.48, 95%CI: 1.36-8.92, P = 0.009), invasion grade(T3-4 vs T1-2: OR = 2.86; 95%CI: 1.69-4.83; P = 0.000), and lymph node metastasis(positive vs negative: OR = 2.64; 95%CI: 1.33-5.27; P = 0.006), it was found that CDH17 showed more positive expressions in each of the more severe cases. Country-stratified analyses from all four experimental subgroups showed that high CDH17 expression levels may be related to GC among Chinese and Korean populations(all P < 0.05), with the exception of the invasion grade T3-4 vs T1-2 comparison, where the relation only held among the Chinese population(OR = 2.86, 95%CI: 1.69-4.83, P = 0.000). CONCLUSION: Collectively, the data reflects the capacity of CDH17 in tumor proliferation and metastasis among GC patients.

关键词： cadherin 17 Protein expression Gastric cancer Meta

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Quantification of the stiffness and strength of cadherin ectodomain binding with different ions

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Theoretical & Applied Mechanics Letters 2014年第3期4卷 55-60页

作者： Zhiyang Xu Dechang Li Baohua Ji Biomechanics and Biomaterials Laboratory Department of Applied MechanicsBeijing Institute of Technology

The stiffness and strength of extracellular （EC） region of cadherin are proposed to be two important mechanical properties both for cadherin as a mechanotransductor and for the formation of cell-cell adhesion. In this study, we quantitatively characterized the stiffness and strength of EC structure when it binds with different types of ions by molecular dynamics simulations. Resuits show that EC structure exhibits a rod-like shape with high stiffness and strength when it binds with the bivalent ions of calcium or magnesium. However, it switches to a soft and collapsed conformation when it binds with the monova- lent ions of sodium or potassium. This study sheds light on the important role of the bivalent ions of calcium in the physiological function of EC.

关键词： cell-cell adhesion cadherin stiffness strength molecular dynamic simulations

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Expression and Characterization of the Intracellular Part of Receptor Protein Tyrosine Phosphatase PTPRU

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Chemical Research in Chinese Universities 2011年第3期27卷 474-477页

作者： WANG Shao-feng HAN Jia-yu FU Xue-qi ZHAO Zhi zhuang Joe Edmond H. Fischer Signal Transduction Laboratory College of Life Sciences Jilin University Changehun 130012 P. R. China Department of Pathology University of Oklahoma Health Sciences Center Oklahoma City Oklahoma 73104 USA

PTPRU is an MAM domain-containing receptor-like protein tyrosine phosphatase. Previous studies have demonstrated an important role of the enzyme in the maintenance of epithelial integrity and in the regulation of the Wnt/β-catenin signaling pathway. To better understand the function of PTPRU, we cloned and expressed the intracellular portion of PTPRU as a GST fusion protein in E. coli cells. We purified the protein to homogeneity and used it to immunize mice for antibody production. The resultant antibody specifically recognized PTPRU over-expressed in the cell line. Western blot analyses demonstrated the partition of truncated forms of PTPRU containing the cadherin-like domain in the Triton X-100-insoluble fraction, and immunofluorescent cell staining revealed the localization of these proteins in punctate intracellular structures. Our data suggest that the cadherin-like domain of PTPRU has a major role in determining its intracellular localization.

关键词： Tyrosine phosphatase cadherin Catenin Microdomain

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