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Plasma ScF/c-kit Levels in Patients with Dipper and Non-Dipper Hypertension

chinese Medical Sciences Journal 2017年第4期32卷 232-238页

作者： Hailan Zhong chongli Xu Guangsheng chen Xiumei chen Department of cardiology Jiangning Hospital of Nanjing Nanjing 211100 China Department of cardiology First Affiliated Hospital Nanjing Medical University Nanjing 210029 China

Objective The aim of this study was to investigate the relationship between peripheral plasma stem cell factor (ScF)/c-kit levels and the types of dipper and non-dipper hypertension in hypertensive patients.Methods This cross-sectional study included newly diagnosed hypertensive patients who underwent 24-hour ambulatory blood pressure monitor (ABPM) between January 2009 and 2012 in Jiangning city. Patients were divided into the dipper group and the non-dipper group according to ABPM measurements. The levels of ScF and its receptor c-kit, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) in peripheral blood were measured via enzyme-linked immunosorbent assays. The serum levels of glucose and lipid were examined as well. The levels of ScF/c-kit were compared between the dippers and the non-dippers; and their correlation with 24-hour mean systolic blood pressure (MSBP), 24-hour mean diastolic blood pressure (MDBP), TNF-αand IL-6 were investigated using linear regression analyses statistically.Results A total of 247 patients with newly diagnosed hypertension were recruited into the study, including 116 non-dippers and 131 dippers. The levels of peripheral plasma ScF were higher in non-dipper group (907.1±52.7 ng/L vs. 778.7±44.6 ng/L; t=2.837, Pc-kit were higher in non-dipper group too (13.2±1.7 μg/L vs 9.57±1.4 μg/L; t=2.831, PcF/ckit levels were significantly positively correlated with MSBP, MDBP, plasma TNF-α, and IL-6 levels (all Pconclusions Peripheral plasma ScF/c-kit levels are higher in patients with non-dipper hypertension than those with dipper one, and significantly correlate with 24-hour MSBP, 24-hour MDBP, serum TNF-α and IL-6 levels.

关键词： ambulatory blood pressure monitor dipper hypertension non-dipper hypertension stem cell factor c-kit

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益生菌组合物对慢传输型便秘的改善作用

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食品与发酵工业 2022年第11期48卷 95-100页

作者：陈家伦张万祥甘聃仙乐健康科技股份有限公司广东汕头515041

探究益生菌组合物(鼠李糖乳杆菌LR-168、嗜酸乳杆菌LA-99和动物双歧杆菌BB-115)对慢传输型便秘的作用及机制。将50只6周龄雄性BABL/c小鼠随机分为空白组(生理盐水)、模型组(洛哌丁胺)、阳性对照组(洛哌丁胺+动物双歧杆菌BB-12:5×10^(8)... 详细信息

探究益生菌组合物(鼠李糖乳杆菌LR-168、嗜酸乳杆菌LA-99和动物双歧杆菌BB-115)对慢传输型便秘的作用及机制。将50只6周龄雄性BABL/c小鼠随机分为空白组(生理盐水)、模型组(洛哌丁胺)、阳性对照组(洛哌丁胺+动物双歧杆菌BB-12:5×10^(8) cFU)、低剂量组(洛哌丁胺+益生菌组合物:5×10^(8) cFU)和高剂量组(洛哌丁胺+益生菌组合物:5×10^(9) cFU),灌胃21 d后处死,对小鼠生理生化指标进行测定。与模型组相比,益生菌组合物低、高剂量组显著提高小肠推进率、黑便粒数和黑便质量,显著降低首粒黑便时间;此外,灌菌处理后,血清和结肠组织的兴奋性神经递质(P物质、胃动素和胃泌素)分泌上升,抑制性神经递质(血管活性肽、生长抑素和内皮素-1)分泌下降;各组间的血清细胞因子水平无统计学差异;结肠组织的AQP3和c-kit基因转录量在益生菌干预后显著提高。益生菌组合物具有缓解慢传输型便秘的作用,这可能是通过影响胃肠调节肽分泌,以及AQP3和c-kit基因转录的水平来实现的。

关键词：慢传输型便秘益生菌洛哌丁胺胃肠调节肽 AQP3 c-kit

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大鼠冠状动脉粥样硬化钙化组织与c-kit^(+)和sca-1^(+)细胞的关系研究

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中国临床解剖学杂志 2023年第4期41卷 453-458页

作者：孙攀文郭永龙贾阳阳孙昌业郭志坤新乡医学院河南省医用组织再生重点实验室河南新乡453003 新乡医学院第一附属医院心内科河南新乡453003

目的探讨大鼠冠状动脉粥样硬化钙化区域sca-1、c-kit的表达变化,为进一步研究冠状动脉粥样硬化机制奠定细胞学基础。方法30只健康成年SD大鼠,随机分为正常对照组(n=8)和动脉粥样硬化钙化模型组(n=22)。采用丙基硫氧嘧啶灌胃、高脂高胆... 详细信息

目的探讨大鼠冠状动脉粥样硬化钙化区域sca-1、c-kit的表达变化,为进一步研究冠状动脉粥样硬化机制奠定细胞学基础。方法30只健康成年SD大鼠,随机分为正常对照组(n=8)和动脉粥样硬化钙化模型组(n=22)。采用丙基硫氧嘧啶灌胃、高脂高胆固醇饲料喂养和维生素D3腹腔注射方法制备模型。模型制备成功后,通过免疫组织化学和免疫荧光技术观察sca-1、c-kit在模型组与正常组的大鼠冠状动脉组织中的表达变化。利用免疫荧光双标技术证明sca-1与α-SMA的共定位表达。利用western blotting技术检测模型组与正常组的冠状动脉组织c-kit、sca-1蛋白的表达差异。结果造模12周,大鼠冠状动脉管壁明显增厚,内膜明显增生,平滑肌纤维排列显著紊乱,坏死物质沉积、脂质沉积和钙结节沉淀。免疫组化和免疫荧光结果显示sca-1+细胞和c-kit^(+)细胞在动脉粥样硬化钙化区域的冠状动脉内膜、中膜、外膜中数量明显高于正常对照。病变区域的部分细胞存在sca-1与α-SMA的共表达。Western blotting结果显示动脉粥样硬化和钙化区域的c-kit、sca-1蛋白的表达量高于正常对照组。结论sca-1+细胞和c-kit^(+)细胞有向冠状动脉粥样硬化钙化区域迁移的倾向,其机制尚需研究。

关键词： sca-1 c-kit 动脉粥样硬化钙化免疫组织化学大鼠心脏

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coexistence of a c-kit negative gastrointestinal stromal tumor and a gastric mucinous adenocarcinoma

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chinese Medical Journal 2010年第24期123卷 3728-3730页

作者： ZHANG Hao ZHANG Shui-long XU Hui-mian Department of Surgical Oncology First Affiliated Hospital of China Medical University Shenyang Liaoning 110001 China Department of colorectal Oncology Liaoning Cancer Hospital and Institute Shenyang Liaoning 110042 China

Mazur and clark introduced the term ＂gastrointestinal stromal tumor＂（GIST）. These tumors are thought to originate from mesenchymal stem cells that differentiate toward the interstitial cells of cajal. Activation of c-kit or platelet-derived growth factor receptor alpha （PDGFRA） gene mutation has been shown to be a major force in GIST pathogenesis leading to down-stream phosphorylation of substrate proteins and subsequently activation of networks of signal transduction pathways that regulate cell proliferation,survival, apoptosis, motility and other important cell functions. Immunohistochemically, a great majority of GISTs show strong, diffuse c-kit expression. The diagnosis of GIST is currently based on morphologic features and immunohistochemical demonstration of c-kit （cD117）. However, in some cases c-kit immunoreactivity is weak or undetectable.

关键词： gastrointestinal stromal tumor gastric adenocarcinoma c-kit platelet-derived growth factor receptor alpha mutation

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大鼠冠状动脉粥样硬化钙化组织与c-kit和sca-1细胞的关系研究

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中国临床解剖学杂志 2023年

作者：孙攀文郭永龙贾阳阳孙昌业郭志坤新乡医学院第一附属医院心内科新乡医学院河南省医用组织再生重点实验室

目的探讨大鼠冠状动脉粥样硬化钙化区域sca-1、c-kit的表达变化，为进一步研究冠状动脉粥样硬化机制奠定细胞学基础。方法 30只健康成年SD大鼠，随机分为正常对照组（n=8）和动脉粥样硬化钙化模型组（n=22）。采用丙基硫氧嘧啶灌胃、... 详细信息

目的探讨大鼠冠状动脉粥样硬化钙化区域sca-1、c-kit的表达变化，为进一步研究冠状动脉粥样硬化机制奠定细胞学基础。方法 30只健康成年SD大鼠，随机分为正常对照组（n=8）和动脉粥样硬化钙化模型组（n=22）。采用丙基硫氧嘧啶灌胃、高脂高胆固醇饲料喂养和维生素D3腹腔注射方法制备模型。模型制备成功后，通过免疫组织化学和免疫荧光技术观察sca-1、c-kit在模型组与正常组的大鼠冠状动脉组织中的表达变化。利用免疫荧光双标技术证明sca-1与α-SMA的共定位表达。利用western blotting技术检测模型组与正常组的冠状动脉组织c-kit、sca-1蛋白的表达差异。结果造模12周，大鼠冠状动脉管壁明显增厚，内膜明显增生，平滑肌纤维排列显著紊乱，坏死物质沉积、脂质沉积和钙结节沉淀。免疫组化和免疫荧光结果显示sca-1+细胞和c-kit+细胞在动脉粥样硬化钙化区域的冠状动脉内膜、中膜、外膜中数量明显高于正常对照。病变区域的部分细胞存在sca-1与α-SMA的共表达。Western blotting结果显示动脉粥样硬化和钙化区域的c-kit、sca-1蛋白的表达量高于正常对照组。结论sca-1+细胞和c-kit+细胞有向冠状动脉粥样硬化钙化区域迁移的倾向，其机制尚需研究。

关键词： sca-1 c-kit 动脉粥样硬化钙化免疫组织化学大鼠心脏

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Effect of cholesterol on in vitro cultured interstitial cajal-like cells isolated from guinea pig gallbladders

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World Journal of Gastrointestinal Surgery 2020年第5期12卷 226-235页

作者： Bei-Bei Fu Jin-Huang Xu Shuo-Dong Wu Ying Fan Department of Health Management Shengjing Hospital of China Medical UniversityShenyang 110004Liaoning ProvinceChina Department of General Surgery Shengjing Hospital of China Medical UniversityShenyang 110004Liaoning ProvinceChina

BAcKGROUND Loss and/or dysfunction of interstitial cajal-like cells(IcLcs)in the gallbladder may promote cholesterol gallstone formation by decreasing gallbladder motility.AIM To study the effect of cholesterol on the proliferation and apoptosis of IcLcs from guinea pig gallbladders.METHODS Guinea pig gallbladder IcLcs were isolated and cultured in vitro.The cells were exposed to cholesterol solutions at different concentrations(0,25,50,and 100 mg/L)for 24 h.Then,cell proliferation was detected by the ccK-8 method and the apoptosis rate was detected by flow cytometry.Further,the expression of the c-kit protein was detected by Western blot and the expression level of c-kit m RNA in the cells was detected by real-time quantitative PcR.RESULTS After IcLcs were cultured with cholesterol at concentrations of 25,50,and 100 mg/L,the proliferation rates decreased significantly(Pcreased significantly(Pckit protein and m RNA decreased significantly(PcONcLUSION High cholesterol concentrations can inhibit the proliferation of IcLcs and promote apoptosis.This decrease in the IcLc proliferation rate might be caused by the inhibition of the stem cell factor/c-kit signaling pathway.

关键词： Interstitial cajal-like cells cholesterol Apoptosis c-kit

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枳实通降颗粒通过干预MK2磷酸化调控c-kit表达防治术后炎性肠梗阻的机制研究

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世界科学技术-中医药现代化 2022年第12期24卷 4977-4983页

作者：莫黎李倩周平兰王华帅李莹黄丽何永恒湖南中医药大学第二附属医院长沙410005 湖南中医药大学研究生院长沙410208 湖南省中医药研究院附属医院长沙410006

目的研究枳实通降颗粒对术后炎性肠梗阻模型大鼠小肠组织中MAPK激活蛋白激酶2(MAPK-activated protein kinase 2,MK2)、磷酸化MAPK激活蛋白激酶2(p-MK2)、炎症因子IL-6、IL-8、TNF-α及原癌基因(c-kit)的影响,探讨其作用机制。方法先将... 详细信息

目的研究枳实通降颗粒对术后炎性肠梗阻模型大鼠小肠组织中MAPK激活蛋白激酶2(MAPK-activated protein kinase 2,MK2)、磷酸化MAPK激活蛋白激酶2(p-MK2)、炎症因子IL-6、IL-8、TNF-α及原癌基因(c-kit)的影响,探讨其作用机制。方法先将大鼠随机分为空白组、假手术组、模型组、枳实通降颗粒4.2 g·kg^(-1)、8.3 g·kg^(-1)、16.6 g·kg^(-1)组和普芦卡必利0.18 mg·kg^(-1)组,药物干预组于造模术后6 h灌胃给药1次,其余各组以生理盐水代替,术后24 h取材。ELISA法测定各组大鼠小肠组织中炎症因子的含量;Western blot检测小肠组织中MK2、p-MK2和c-kit的蛋白表达情况;RT-PcR检测MK2和c-kit mRNA的表达情况;免疫组化观察c-kit阳性细胞情况。结果与假手术组及空白组比较,模型组大鼠肠道推进率及c-kit蛋白、阳性细胞、mRNA的表达水平均明显偏低,而胃内容物残留率及小肠组织中炎症因子含量、p-MK2蛋白的表达水平则明显偏高(Pc-kit蛋白及mRNA、阳性细胞的表达水平均明显升高,而胃内容物残留率及小肠组织中炎症因子含量、p-MK2蛋白表达水平则明显偏低(P0.05)。结论枳实通降颗粒能显著促进术后炎性肠梗阻模型大鼠的胃肠功能恢复,其作用机制是通过干预MK2磷酸化,抑制肠壁组织的炎症反应,增加c-kit表达水平来实现的。

关键词：术后炎性肠梗阻大鼠枳实通降颗粒 MK2 p-MK2 c-kit

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The expression of c-kit and proliferating cell nuclear antigen in oval cells of rats with hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2003年第4期2卷 537-544页

作者： chi-Hua Fang Wei Zhang Xin-Yong Zhu Jia-Qing Gong Gang-Qing Zhang the Department of Hepatobiliary Surgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, china Department of Hepatobiliary Surgery Zhujiang Hospital First Military Medical University Guangzhou 510282 China. fch58520@***

OBJEcTIVE: To study the relationship between oval cells and primary hepatocarcinoma and the expression of c-kit and proliferating cell nuclear antigen (PcNA) in oval cells of rats with hepatocellular carcinoma. METHODS: A hundred and twenty clean SD rats were divided into three groups: normal group, cancer-induction group and intervention group. The normal group was fed with standard forage while the rest two groups were fed with 3'-methyl-2-methylamino-azobenzene (DAB) to induce carcinoma for 14 weeks and then fed with standard forage and water. Uscharidin was injected abdominally to the intervention group from the first week to the 14th week. All rats were killed and biopsy specimens were taken from the left and right liver lobes for immunohistochemical staining of c-kit and PcNA on the 2nd, 4th, 6th, 8th, 10th, 12th, 14th, 16th, 18th, 20th, 22nd, and 24th week. RESULTS: From the 2nd to 14th week after liver infection, c-kit positive cells, mainly oval cells were found in the portal area in the carcinoma-induction group and dotted positive pigmentations in liver lobules. In the 22nd week, a large number of cancerous nodes occurred and nuclei heteromorphi-m was apparent; the number of positive cell decreased but positive cells could be sparsely observed in cancerous nodes. In the 2nd week of the carcinoma-induction process, PcNA positive cells were oval cells in the portal area. In the 4th week, a lot of hepatic cells were positively stained, especially in the central vein area. In the 6th week, PcNA positive cells could be seen in the lobules of the liver. In the 8th week, the number of PcNA cells decreased comparatively. From the 10th to 14th week, oval cells in the portal area were still over-expressed. From the 16th to 24th week, a large number of cancerous nodes occurred and PcNA was over-expressed in some of them. In necrotic cancerous nodes, the para-cancerous PcNA positive cells were sparsely distributed and their number was less than that of PcNA positive cells of cancerous tissues. cONcLUSIONS: Hepatic

关键词： hepatocellular carcinoma oval cell proliferating cell unclear antigen c-kit

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Association between kitLG Gene Polymorphisms and Testicular Germ cell Tumors:A Systematic Review and Meta-analysis

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current Medical Science 2019年第5期39卷 702-706页

作者： Jia-xuan QIN Zong-long cAI Yu-feng YANG Jin-chun XING Xuan ZHUANG Department of Urology Surgery Center of Diagnosis and Treatment of Urinary System Diseasesthe Key Laboratory of Urinary Tract Tumors and Calculi of Xiamen Citythe First Affiliated Hospital of Xiamen UniversityXiamen 361003China The First clinical Medical School of Fujian Medical University Xiamen 361003China

It has been reported that c-kit ligand(kitLG)gene polymorphisms may be associated with testicular germ cell tumors(TGcT).Owing to mixed and inconclusive results,we con ducted a systematic review and meta-analysis to summarize and clarify this association.A systematic search of studies on the association between kitLG gene polymorphisms and TGcT susceptibility was conducted in databases.Odds ratios and 95%confidence intervals were used to pool the effect size.Six articles were included in our systematic review and meta-analysis.compared with adenine(A),kitLG rs995030 guanine(G)might be associated with increased risk of TGcT.There are insufficient data to fully confirm the association between kitLG rs4474514 and TGcT susceptibility.Well-designed studies with larger sample size and more subgroups are required to validate the risk identified in the current meta-analysis.

关键词： c-kit ligand single nucleotide polymorphisms testicular germ cell tumors metaanalysis

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Investigating the effect of Banxia Houpu decoction for the treatment of gastrointestinal dysfunction after multiple fractures: involvement of interstitial cells of cajal

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Gastroenterology & Hepatology Research 2023年第4期5卷 19-25页

作者： Yu Zhang Wei-Qiang Zhao Meng-Qiang Fan Jian-Sheng Qian Hui-Hui Xu Yang Zheng Jiang Hua Li-Pei Wang Jie-Feng Huang Department of Orthopaedics The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006China The Fourth clinical college Zhejiang Chinese Medical UniversityHangzhou 310053China The Third clinical college Zhejiang Chinese Medical UniversityHangzhou 310053China The First clinical college Zhejiang Chinese Medical UniversityHangzhou 310053China Department of Pathology Hangzhou Normal University School of Basic Medical SciencesHangzhou 311121China

Background:Fracture is a common large-organ traumatic injury and usually leads to complications in other systems.Multiple fractures are often associated with gastrointestinal(GI)dysfunction.However,no study has evaluated the treatment of GI dysfunction.The purpose of the study is to explore the efficacy and mechanism based on Icc pathway of Banxia-Houpu decoction on GI.Methods:Male Sprague-Dawley mice were randomly divided according to the fracture modeling method and intervention.We detected the“GI residual rate”and motility.Gastric antrum and jejunum tissues were dissected for hematoxylin and eosin staining to observe the structural integrity of the GI tract,and immunohistochemistry was used to detect the expression of c-kit protein.Results:compared with the fracture group the GI residual rates in the Banxia Houpu decoction and mosapride groups were significantly low,while GI motility was significantly high.HE staining revealed significant GI tissue necrosis and inflammatory cell infiltrations in the fracture groups.The Banxia Houpu decoction and mosapride groups,exhibited less pathology.Immunohistochemical staining showed upregulated c-kit protein expression in the fracture groups.The c-kit protein level was decreased in the mosapride group.Additionally,c-kit protein expression in the Banxia Houpu decoction groups was significantly decreased in a concentration-dependent manner.conclusion:Banxia Houpu decoction improves GI dysfunction after multiple fractures,reduces inflammation and necrosis of gastric epithelial cells,and inhibits c-kit protein expression in GI tissues of mice.Results showed revealed one of the mechanisms underlying the effects of this decoction on the GI dysfunction in mice after multiple fractures.

关键词： Banxia Houpu decoction c-kit gastrointestinal diseases multiple fractures animal model

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