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Astragalus injection inhibits c-jun n terminal kinase mrna expression following oxygen-glucose deprivation and reintroduction in rat hippocampal neurons

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neural Regeneration Research 2009年第11期4卷 879-884页

作者： Dongqing Ye Weijuan Gao Fengxia Yan Tao Qian Yali Zhang Research Room of Pathophysiology Chengde Medical College Chengde 067000 Hebei Province China Affiliated Hospital of chengde Medical college Chengde 067000 Hebei Province China

BAcKGROUnD： In studies concerning cell injury induced by cerebral ischemia-reperfusion, current experiments have primarily focused on altered protein levels. In addition, the apoptotic proteins Bax and Bcl-2 have been thoroughly studied with regard to initiating neuronal apoptosis. OBJEcTIVE： To establish an in vitro model of oxygen-glucose deprivation and reintroduction in the rat hippocampus to simulate cerebral ischemia-reperfusion injury; to observe c-jun n-terminal kinase 3 （JnK3） mrna expression in hippocampal neurons following Astragalus injection; and thus to determine changes in the signaling and downstream pathways of neuronal apoptosis at the cellular and molecular level. DESIGn, TIME AnD SETTInG： A randomized, controlled, cellular and molecular experiment was performed at the Department of central Laboratory, chengde Medical college from February to june 2008. MATERIALS： Astragalus injection, the main ingredient of astragaloside, was purchased from chengdu Di＇ao Jiuhong Pharmaceutical Manufactory, china. JnK3 mrna probe and in situ hybridization kit were purchased from Tianjin Haoyang Biological Technology, china, and JnK3 RT-PcR primers were designed by Shanghai Bio-engineering, china. METHODS： Primary cultures of hippocampal neurons derived from Sprague Dawley rats, aged 1 2 days, were established. After 8 days, the hippocampal neurons were assigned to the following interventions： model group, Astragalus group, and vehicle control group, cells were subjected to oxygen-glucose reintroduction after oxygen-glucose deprivation for 30 minutes in sugar-free Earle＇s solution and a hypoxia device, which contained high-purity nitrogen. The normal control group was subjected to primary culture techniques and was not treated using above-mentioned interventions. In addition, the Astragalus and vehicle control groups were treated with Astragalus injection （0.5 g/L raw drug） or sterile, deionized water at 2 hours prior to oxygen-glucose deprivation, respectively. MA

关键词： oxygen-glucose deprivation and reintroduction Astragalus injection c-jun n-terminalkinase 3 mrna hippocampal neuron

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