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Rice science 2024年第3期31卷 317-327,I0042-I0045页

作者： LI Dian DUAN Wenjing LIU Qun’en WU Weixun ZHAN Xiaodeng SUN Lianping ZHANG Yingxin CHENG Shihua State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China

Accurate genomic information is essential for advancing genetic breeding research in specific rice varieties.This study presented a gapless genome assembly of the indica rice cultivar Zhonghui 8015(ZH8015)using Pac Bio HiFi,Hi-C,and ONT(Oxford Nanopore Technologies)ultra-long sequencing technologies,annotating 43037 gene structures.Subsequently,utilizing this genome along with transcriptomic and metabolomic techniques,we explored ZH8015's response to brown planthopper(BPH)infestation.Continuous transcriptomic sampling indicated significant changes in gene expression levels around 48 h after BPH feeding.Enrichment analysis revealed particularly significant alterations in genes related to reactive oxygen species scavenging and cell wall formation.Metabolomic results demonstrated marked increases in levels of several monosaccharides,which are components of the cell wall and dramatic changes in flavonoid contents.Omics association analysis identified differentially expressed genes associated with key metabolites,shedding light on ZH8015's response to BPH infestation.In summary,this study constructed a reliable genome sequence resource for ZH8015,and the preliminary multi-omics results will guide future insect-resistant breeding research.

关键词： brown planthopper gapless genome genome assembly multi-omics Nilaparvata lugens rice

Diversity and dynamics of microbial communities in brown planthopper at different developmental stages revealed by high-throughput amplicon sequencing

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Insect Science 2020年第5期27卷 883-894页

作者： Zheng-Liang Wang Tian-Zhao Wang Hang-Feng Zhu Hai-Bo Pan Xiao-Ping Yu Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine College of Life SciencesChina Jiliang UniversityHangzhouChina

The microbiome associated with brown planthopper(BPH)plays an important role in mediating host health and fitness.Characterization of the microbial community and its structure is prerequisite for understanding the intricate symbiotic relationships between microbes and host insect.Here,we investigated the bacterial and fungal communities of BPH at different developmental stages using high-throughput amplicon sequencing.Our results revealed that both the bacterial and fungal communities were diverse and dynamic during BPH development.The bacterial communities were generally richer than fungi in each developmental stage.At 97%similarly,19 phyla and 278 genera of bacteria were an-notated,while five fungal phyla comprising 80 genera were assigned.The highest species richness for the bacterial communities was detected in the nymphal stage.The taxonomic diversity of the fungal communities in female adults was generally at a relatively higher level when compared to other developmental stages.The most dominant phylum of bacteria and fungi at each developmental stage all belonged to Proteobacteria and Ascomycota,re-spectively.A significantly lower abundance of bacterial genus Acinetobacter was recorded in the egg stage when compared to other developmental stages,while the dominant fun-gal genus Wallemia was more abundant in the nymph and adult stages than in the egg stage.Additionally,the microbial composition differed between male and female adults,suggesting that the microbial communties In BPH were gender-dependent.Uverall,our study enriches our knowledge on the microbial communities associated with BPH and will provide clues to develop potential biocontrol techniques against this rice pest.

关键词： amplicon sequencing brown planthopper dynamics microbial diversity microbiome

Towards Understanding of Molecular Interactions between Rice and the brown planthopper

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Molecular Plant 2013年第3期6卷 621-634页

作者： Xiaoyan Cheng Lili Zhu Guangcun He State Key Laboratory of Hybrid Rice College of Life Sciences Wuhan University Wuhan 430072 People's Republic of China

The brown planthopper （BPH） is the most notorious pest of rice （Oryza sativa）. Studies of rice-BPH interaction have contributed to development of new rice varieties, offering an effective means for long-lasting control of BPH. Here, we review the status of knowledge of the molecular basis of rice-BPH interaction, from the perspective of immunity. The BPH has complicated feeding behaviors on rice, which are mainly related to host resistance. Now, 24 resistance genes have been detected in rice, indicating gene-for-gene relationships with biotypes of the BPH. However, only one BPH resistance gene （Bph14） was identified and characterized using map-based cloning. Bph14 encodes an immune receptor of NB-LRR family, providing a means for studying the molecular mechanisms of rice resistance to BPH. Plant hormones （e.g. salicylic acid and jasmonate/ethylene）, Ca2＋, mitogen-activated protein kinases （MAPKs）, and OsRacl play important roles in the immune response of rice to BPH. Signal transduction leads to modifying expression of defense- related genes and defense mechanisms against BPH, including sieve tube sealing, production of secondary metabolites, and induction of proteinase inhibitor. A model for the molecular interactions between rice and the BPH is proposed, although many details remain to be investigated that are valuable for molecular design of BPH-resistant rice varieties.

关键词： rice brown planthopper innate immunity BPH-feeding cascade.

Genomic resources for the brown planthopper, Nilaparvata lugens： Transcriptome pyrosequencing and microarray design

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Insect Science 2012年第1期19卷 1-12页

作者： Chris Bass Martin Bay Hebsgaard Joseph Hughes Centre for Sustainable Pest and Disease Management Rothamsted Research Harpenden University of Edinburgh Institute of Evolutionary Biology Kings Buildings Edinburgh Institute of Biodiversity Animal Health and Comparative Medicine College of Medical Veterinary and Life Sciences University of Glasgow Glasgow United Kingdom

The brown planthopper, Nilaparvata lugens is a pest of cultivated rice through- out Asia and is controlled using insecticides and/or resistant rice varieties. This species has developed resistance to many classes of insecticide and biotypes have developed that are vir- ulent against formerly resistant rice cultivars. Insects use a suite of detoxification enzymes, including cytochrome P450s, glutathione S-transferases and carboxyl/cholinesterases to defend themselves against plant secondary metabolites and pesticides. Pyrosequencing on the Roche 454-FLX platform was used to produce a substantial expressed sequence tag （EST） dataset to complement the existing Sanger sequenced ESTs in GenBank. A total of 78 959 reads were combined with the 37 392 publically available Sanger ESTs; these assembled into 8 911 contigs and 10 620 singletons. Analysis of the distribution of tenta- tive unique genes （TUGs） with the gene ontology for biological processes and molecular functions suggests that the 454 and Sanger EST assembly is broadly representative of the N. lugens transcriptome. The brown planthopper transcriptome was found to contain 31 TUGs encoding P450s, nine encoding glutathione S-transferases and 26 encoding car- boxyl/cholinesterases and many of these are putatively involved in the detoxification of xenobiotics. The Agilent eArray platform was used to construct an oligonucleotide mi- croarray populated with probes for ~ 19 000 unigene sequences, including all those known to encode detoxification enzymes. The genomic resources developed in this study will be useful to the community studying this crop pest and will help elucidate the molecular mechanism underlying insecticide resistance and planthopper adaptation to resistant rice cultivars.

关键词： brown planthopper detoxification enzymes insecticide resistance Nilaparvata lugens transcriptome sequencing

Determining the geographic origin of the brown planthopper, Nilaparvata lugens, using trace element content

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Insect Science 2012年第1期19卷 21-29页

作者： Qi Peng Qi-Yi Tang Jia-Lun Wu Qing-Ling Miao Jia-An Cheng State Key Laboratory of Rice Biology Institute of Insect SciencesZhejiang UniversityHangzhouChina

bstract A chemometric study using pattern recognition technology was carried out to characterize the geographic origins of the brown planthopper, Nilaparvata lugens. The concentrations of 23 trace elements （Mn, Mo, Cd, Ce, V, Th, Cs, Be, T1, Fe, Nd, Pr, Se, Tm, Lu, Eu, Ho, Br, Dy, Gd, U, Sm and Er） in 53 samples from seven regions in southern China were determined using inductively coupled plasma mass spectrometry. The data obtained were successively evaluated using a multivariate statistical approach, namely, linear discriminant analysis, which allowed classification and discrimination of the N. lugens samples from Fuqing, Shaoguan, Hepu, Yongfu, Hengnan, Wan-an and Yongkang with high accuracy and a clear separation among the seven regions. The results show that pattern identification on the basis of trace elements in the bodies ofN. lugens is feasible for determining the geographic origins of individuals.

关键词： brown planthopper discriminant analysis forecasting geographic origin trace element

Understanding brown planthopper Resistance in Rice: Genetics, Biochemical and Molecular Breeding Approaches

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Rice science 2021年第6期28卷 532-546页

作者： Lakesh MUDULI Sukanta Kumar PRADHAN Abinash MISHRA Debendra Nath BASTIA Kailash Chandra SAMAL Pawan Kumar AGRAWAL Manasi DASH Department of Plant Breeding and Genetics College of AgricultureOdisha University ofAgriculture and TechnologyBhubaneswar 751003OdishaIndia Department of Bioinformatics Centre for Post Graduate StudiesOdisha University of Agriculture and TechnologyBhubaneswar 751003OdishaIndia Department of Agricultural Biotechnology College of AgricultureOdisha University of Agriculture and TechnologyBhubaneswar 751003OdishaIndia

brown planthopper(BPH,Nilaparvata lugens St自I)is the most devastating pest of rice in Asia and causes significant yield loss annually.Around 37 BPH resistance genes have been identified so far in indica,African rice varieties along with wild germplasms such as Oryza officinalis,O.minuta,O.nivara,O.punctata,O.rufipogon and O,latifolia.Genes/QTLs involved in BPH resistance,including Bph1,bph2/BPH26,Bph3,Bph6,bph7,BPH9,Bph12,Bph14,Bph15,Bph17,BPH18,bph19,Bph20,Bph21(t),Bph27,Bph27©Bph28(t),BPH29,QBph3,QBph4,QBph4.2,Bph30,Bph32,Bph33,Bph35 and Bph36,have been fine-mapped by different researchers across the globe.The majority of genes/QTLs are located on rice chromosomes 1,3,4,6,11 and 12.Rice plants respond to BPH attack by releasing various endogenous metabolites like proteinase inhibitors,callose,secondary metabolites(terpenes,alkaloids,flavonoid,etc.)and volatile compounds.Besides that,hormonal signal pathways mediating(antagonistic/synergistic)resista nee responses in rice have been well studied.Marker-assisted breedi ng and genome editi ng techniq ues can also be adopted for improving resista nee to novel BPH biotypes.

关键词： rice brown planthopper resista nee wild germplasm marker-assisted breedi ng genome editing secondary metabolite

Pyramiding blast,bacterial blight and brown planthopper resistance genes in rice restorer lines

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Journal of Integrative Agriculture 2016年第7期15卷 1432-1440页

作者： JI Zhi-juan Yang Shu-dong ZENG Yu-xiang LIANG Yan YANG Chang-deng QIAN Qian College of Agronomy Shenyang Agricultural UniversityShenyang 110866P.R.China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006P.R.China Shengzhou Seed Production Farm Shaoxing 312000P.R.China

Rice blast, bacterial blight （BB） and brown planthopper （BPH） are the three main pests of rice. This study investigated pyr-amiding genes resistant to blast, BB and BPH to develop restorer lines. Ten new lines with blast, BB and/or BPH resistance genes were developed using marker-assisted selection （MAS） technique and agronomic trait selection （ATS） method. Only HR13 with resistance genes to blast, BB and BPH was obtained. In addition to blast and BB resistance, four lines （HR39, HR41, HR42, HR43） demonstrated moderate resistance to BPH, but MAS for BPH resistance genes were not conducted in developing these four lines. These data suggested that there were unknown elite BPH resistance genes in the Zhongzu 14 donor parent. A more effective defense was demonstrated in the lines withPi1 andPi2 genes although the weather in 2012 was favorable to disease incidence. Blast resistance of the lines with a single resistance gene,Pita, was easily inlfuenced by the weather. Overal, the information obtained through pyramiding multiple resistance genes on developing the restorer lines is helpful for rice resistance breeding.

关键词： rice blast bacterial blight brown planthopper resistance pyramid

Biochemical studies on malathion resistance, inheritance and association of carboxylesterase activity in brown planthopper, Nilaparvata lugens complex in Peninsular Malaysia

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Insect Science 2010年第6期17卷 517-526页

作者： M. A. Latif Mohd Yosuh Omar Soon Guan Tan S.S. Siraj Abdul Rahim Ismail Department of Crop Science Faculty of Agriculture Department of Biology Faculty of Science Department of Cell and Molecular Biology Faculty of Biotechnology and Molecular Science Department of Aquaculture Faculty of Agriculture Universiti Putra Malaysia Serdang Selangor Malaysia

Two sympatric populations of brown planthopper （BPH）, one from rice and the other from Leersia hexandra were collected from each of five locations in Malaysia. All the tested malathion-resistant individuals of the rice BPH population and F1 generation （cross between malathion-resistant [usually caught on rice] and malathion- susceptible [usually caught on Leersia]） showed high esterase activity, while all malathion- susceptible individuals on L. hexandra showed low esterase activity. In the F2 generation, all the individuals tested against malathion were approximately 75% resistant and 25% susceptible and the inheritance pattern of esterase activity （high and low esterase activity） segregated in the same manner to a 3：1 ratio. This confirms that resistance to malathion is mono-factorial and inheritance pattern ofesterase activity is also linked to malathion resistance. Carboxylesterase or total esterase activity in BPH is inherited in a simple Mendelian fashion that is encoded by a single dominant gene. For the total esterase assay, average esterase activity levels in the rice-infesting population ranged from 17.64 to 19.37 nmoles 1-napthol/mg protein while that in the Leersia-infesting population ranged from 5.29 to 6.11 nmoles 1-napthol/mg protein. In terms of esterase activity, the two sympatric Nilaparvata lugens populations separated into two distinct groups. Results based on the tube color intensity test showed 96% and 98% resistant and susceptible individuals were present in the rice- and Leersia-infesting populations, respectively. In a filter paper test, the rice-infesting population had 94% with high esterase activity while the Leersia-infesting population had 96% with low esterase activity.

关键词： brown planthopper carboxylesterase assay inheritance study insecticide resistance rice

Effects of Temperature on Functional Response of Anagrus nilaparvatae Pang et Wang (Hymenoptera:Mymaridae) on the Eggs of Whitebacked planthopper,Sogatella furcifera Horvth and brown planthopper,Nilaparvata lugens Stl

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Journal of Integrative Agriculture 2012年第8期11卷 1313-1320页

作者： MA Ming-yong PENG Zhao-pu HE Yuan Institute of Plant Protection Hunan Academy of Agricultural Sciences Changsha 412002 P.R.China Key Laboratory of Monitoring and Management of Plant Diseases and lnsects Ministry of Agriculture Nanjing Agricultural University Nanjing 210095 P.R. China

Understanding the temperature affecting parasitic efficiency is critical to succeed in utilizing parasitoid as natural enemy in pest management. Laboratory studies were carried out to determine the effects of temperature on parasitoid preference of female Anagrus nilaparvatae Pang et Wang （Hymenoptera：Mymaridae） to the eggs of whitebacked planthopper （WBPH）, Sogatella furcifera Horváth and brown planthopper （BPH）, Nilaparvata lugens Stl to build a composite model describing changes in parasitic response along a temperature gradient （18, 22, 26, 30, 34°C）. The results showed that attack responses of A. nilaparvatae on WBPH and BPH were the best described by a Type II functional response. The two parameters, attack rates （a） and handling times （Th）, of A. nilaparvatae to both eggs were influenced by the temperature. The maximum attack rates to WBPH （1.235） and BPH （1.049） were at 26 and 34°C, respectively, and the shortest handling times to WBPH （0.063） and BPH （0.057） were at 30 and 26°C, respectively. However, the optimal temperature for parasitic efficiency of A. nilaparvatae to WBPH and BPH eggs was both at 26°C, which showed that the present microclimate temperature of the habitat in the paddyfield was beneficial to A. nilaparvatae and indicated that parasitic efficiency of A. nilaparvatae would be impaired by global warming.

关键词： functional response temperature Anagrus nilaparvatae whitebacked planthopper brown planthopper

High Temperature Modifies Resistance Performances of Rice Varieties to brown planthopper,Nilaparvata lugens(Stl)

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Rice science 2010年第4期17卷 334-338页

作者： WANG Bao-ju Xu Hong-xing ZHENG Xu-song Fu Qiang Lu Zhong-xian Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Itangzhou 310021 China School of Life and Environment Science Hangzhou Normal University Hangzhou 310036 China China National Rice Research Institute Hangzhou 310006 China

To investigate the effect of temperature on the resistance characteristics of dce varieties with different resistance genes to brown planthopper （BPH）, Nilaparvata lugens （Stal）, the resistances of IR26 （Bphl） and IR36 （bph2） to BPH population in Hangzhou, China were monitored in greenhouse during September in 2007 and 2008 by using the standard seedling screening techniques （SSST） developed by the International Rice Research Institute （IRRI）. Furthermore, the changes in resistance of IR26 and IR36 to BPH, soluble sugar and oxalic acid contents in 25-day-old rice plants of susceptible variety TN1 and resistant varieties IR26 and IR36 were detected at five temperatures （22℃, 25℃, 28℃, 31℃ and 34℃）. IR26 completely lost resistance both in greenhouse and at the five tested temperatures. IR36 still had moderate resistance at natural temperature, but its resistance decreased gradually when the temperature increased from 25℃ to 34℃, and fully lost its resistance at 31℃ and 34℃. The highest durable resistance of IR26 and IR36 were recorded at 25℃. The soluble sugar content in plants of the three tested rice varieties increased with temperature increase, and the oxalic acid content increased with the temperature increase at first, maximized at 25℃, and then declined. Two-way ANOVA indicated significant effects of temperature and rice variety on contents of soluble sugar and oxalic acid in rice plants

关键词： brown planthopper Nilaparvata lugens temperature rice resistant characteristics