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Amino Compounds in Poultry Litter,Litter-Amended Pasture Soils and Grass Shoots

Pedosphere 2014年第2期24卷 178-185页

作者： Z.Q.HE Z.N.SENWO H.X.ZOU I.A.TAZISONG D.A.MARTENS USDA-ARS Southern Regional Research Center Department of Biological and Environmental Sciences Alabama A&M University Department of Chemical and Biological Engineering the University of Maine USDA-ARS Southwest Watershed Research Center

Amino compounds （ACs）, i.e., amino acids and amino sugars, are the major forms of organic nitrogen （N） in animal manure and soil. To increase our understanding on the effect of long-term poultry litter （PL） application on soil AC pools and turnover, in this study, we determined the contents of 21 ACs in 23 PL samples, i5 soil samples collected from 0-20, 20-40, and 40-60 cm layers of five pasture plots with 0, 5, 10, 15 and 20 years of PL applications, and 5 grass shoot samples grown on these pasture fields. The contents of 21 ACs were simultaneously determined by methanesulfonic acid hydrolysis/extraction and anion chromatography-pulsed amperometry. PL application increased soil total and individual AC contents with a distribution pattern similar to that of AC in PL. The highest AC-N concentrations were observed in the soils with 10- or 15-year PL applications, inconsistent with the order of annual application rates or eunmlative applied PL amounts. Application of PL increased the AC contents in grass shoots whereby the highest increase of most ACs was with the shoots from the fields that received PL for 5 years. These observations suggested that both freshly applied and residual PL had contributions to the soil AC-N, and that PL application also accelerated AC-N transformation in soil.

关键词： amino acids amino sugars broiler feed soil nitrogen

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In Vitro Study on Commercial Organic Acid Activate WD (WD) Against Four Pathogenic Bacteria

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Journal of Northeast Agricultural University(English Edition) 2012年第4期19卷 62-65页

作者： Jiang Guo-zheng Li Ji-chang Han Zhen-xing Liu Ting Wang Yuan Cao Hong College of Veterinary Medicine Northeast Agricultural University Novus International Trade Limited Company

To investigate the antibacterial activity of commercial organic acid Activate WD （WD）, the minimal inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） of WD against Staphylococcus aureus, Escherichia coli, Salmonella pullorum, and Campylobacter jejuni were determined by double broth dilution method. Bacteria were added in a mixture of water and commercial broiler feed adjusted by WD into pH 3.0, 4.0, and 5.0; the survival of bacteria was determined by a direct plate count method at 0, 0.5, 1, 4, 8, and 24 h after being mixed to test the bactericidal potential of WD. The results showed that the MIC of WD against four bacteria was 0.3125%, and the MBC was 0.625%. At pH 3.0, bacteria could not survive. Campylobacter jejuni died after 4 h of incubation at pH 4.0, 5.0, whereas for other three bacteria, the bacterium numbers were below detection limits until 8 h of incubation. In conclusion, WD had significant antibacterial activity, and could be used on farms to prevent cross-infection via rearing water.

关键词： organic acid antibacterial activity broiler feed water

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