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Cultivation of Red Sea bream Pagrus Major Lymphocyte

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High Technology Letters 2000年第3期6卷 72-75页

作者：孙修勤

This study attempted to culture red sea bream lymphocytes using artificial medium supplemented with mitogens and a cytokine. Growth of the lymphocytes was compared between cultures with mitogens phytohemagglutinin (PHA), lipopolysaccharide (LPS), concanavalin A(Con A) and a cytokine mouse interleukin 2(IL 2) in a basal medium at various combinations. Addition of PHA or LPS enhanced growth of the lymphocytes, Con A did not enhance growth so much. Large colonies were formed when PA was added. Con A induced scattered single cell proliferation of the lymphocytes. IL 2 supported the actions of PHA and LPS. Combined addition of PHA and IL 2 maintained proliferation for 30 d.

关键词： Red sea bream Lymphocyte cultivation

STUDIES ON THE PHOSPHORUS REQUIREMENT AND PROPER CALCIUM/PHOSPHORUS RATIO IN THE DIET OF THE BLACK SEA bream(SPARUS MACROCEPHALUS)

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Chinese Journal of Oceanology and Limnology 1993年第2期11卷 130-134页

作者：刘镜恪李茂堂王可玲王新成刘建康 Institute of Oceanology Academia Sinica Qingdao 266071 Institute of Hydrobiology Academia Sinica Wuhan 430072

An experiment on the phosphorus requirement and the proper Ca/P ratio in the diet of the black sea bream using the phosphorus gradient method (with casein as basic diet, sodium dihydrogen phosphate as source of phosphorus, and calcium lactate as source of calcium) showed that growth was greatly affected by the diet’s phosphorus content and Ca/P ratio. Inadequate phosphorus in the diet resulted in slow growth and poor food conversion ratio (FCR). Analyses of the fish body showed it contained a high level of lipid but a low level of moisture, ash, calcium and phosphorus. The optimal values of phosphorus and Ca/P ratio in the black sea bream diet are 0.68% and 1:2 respectively. Phosphorus in excess of this optimum value resulted in slow growth or even death. The results of this experiment clearly indicated that phosphorus is the principal mineral additive affecting black sea bream growth.

关键词： phosphorus requirement Ca/P ratio black sea bream

STUDIES ON THE OPTIMUM PROTEIN CONTENT AND THE RATIO OF ANIMAL TO PLANT PROTEIN IN THE DIET OF THE BLACK SEA bream(SPARUS MACROCEPHALUS)

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Chinese Journal of Oceanology and Limnology 1994年第4期12卷 381-384页

作者：刘镜恪王可玲王新成刘建康 Institute of Oceanology Chinese Academy of Sciences Qingdao Institute of Hydrobiology Chinese Academy of Sciences Wuhan

An experiment on the optimum dietary protein content for the black sea bream using semi-purified dietsshowed the optimum content is 50.19% by weight as inferred from the growth rate and food consumptionof the fish. Another experiment on the proper ratio of animal to plant protein in its diet using sandeel as theanimal protein source and peanut cake meal as plant protein source showed that the ratio had great ef-fect on its growth and should be 1.00:0.89—0.96 as judged from the growth rate and food consumptionof the fish.

关键词： protein content black sea bream

Development of Fifteen Novel Microsatellite Markers from Rock bream (<i>Oplegnathus fasciatus</i>)

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Open Journal of Genetics 2014年第4期4卷 287-291页

作者： Jong-Oh Kim Wi-Sik Kim Myung-Joo Oh Department of Aqualife Medicine Chonnam National University Yeosu Korea

Rock bream (Oplegnathus fasciatus) is one of the most economically valuable fish in Korea. In recent years, artificial breeding techniques with molecular and microsatellite markers have been developed to enhance rock bream resources. Microsatellite loci to define genetic diversity were screened in rock bream (n = 30) from Jeju areas of Korea and fifteen polymorphic microsatellite loci were newly identified and analyzed. The number of alleles per locus ranged from 9 to 34 while observed and expected heterozygosity ranged from 0.600 to 1.000 and from 0.772 to 0.977, respectively. These markers will serve as a foundation for future population genetic studies and the selective breeding technology of rock bream farming.

关键词： Oplegnathus fasciatus Rock bream Microsatellite Markers Population Genetics

THE EFFECTS OF RECOMBINANT EEL GROWTH HORMONE,METHYLTESTOSTERONE AND L-THYROXINE ON THE GROWTH OF REDSEAbream,PAGROSOMUSMAJOR

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Chinese Journal of Oceanology and Limnology 1995年第1期13卷 86-96页

作者：徐斌葛国昌苗宏志 Fisheries College Ocean University of Qingdao Qingdao Marine Life Sciences College Ocean University of Qingdao Qingdao

The effects of recombinant eel growth hormone (reGH).methyltestosterone (MT)and L-thyroxine (T4)on the growth of red sea bream. Pagrosomus *** *** of reGH to fry by immersion at 2 mg 1 for 2 hevery 5 dsys resulted in significant increase in both weight and *** the condition factor (CF) diminished relative to that of similarly treated controls over the 37day treatment *** in 0.1 mg:1 T4 also resulted in significant increase in both weight and length and higher survival rate of test fry compared to the controls. Immersion in MT had less effect on growth and high-dose resulted in high *** the second *** of 2 μg reGH(gwk)caused a significant increase in the specific growth rate (SGR) of test red sea bream fingerlings relative to that of the controls during the 4-week treatment period and maintained the increasing trend over the post-treatment period (weeks 4-6).Injection of MT at a dosage of 1μg (gwk) resulted in a significant

关键词： growth hormone. methyltestosterone. L-thyroxine. growth. red sea bream