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Abnormal expressions of proliferating cell nuclear antigen and P27 protein in brain glioma

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Neural Regeneration Research 2007年第10期2卷 591-594页

作者： Yong feng Hu Wei Shi Chong xiao Liu Jian jun Sun Rui zhi Wang Zhen yu Guo Department of Neurosurgery Mianxian Country HospitalMianxian724200Shanxi ProvinceChina Department of Neurosurgery the Second Affiliated Hospital of Medical CollegeXi＇an Jiaotong UniversityXi＇an 710004Shanxi Provincechina

Both proliferating cell nuclear antigen and P27 protein are important factors to regulate cell cycle. While, the combination of them can provide exactly objective markers to evaluate prognosis of patients with brain glioma needs to be further studied based on pathological level. OBJECTIVE： To observe the expressions of proliferating cell nuclear antigen and P27 protein in both injured and normal brain glioma tissues and analyze the effect of them on onset and development of brain glioma. DESIGN： Case contrast observation. SETTING： Department of Neurosurgery, the Second Affiliated Hospital of Xi＇an Jiaotong University. PARTICIPANTS： A total of 63 patients with brain glioma were selected from Department of Neurosurgery, the Second Affiliated Hospital of Xi＇an Jiaotong University from July 1996 to June 2000. There were 38 males and 25 females and their ages ranged from 23 to 71 years. Based on pathological classification and grading standards of brain glioma, patients were divided into grade I - II （n=30） and grade III- IV （n = 33）. All cases received one operation but no radiotherapy and chemiotherapy before operation. Sample tissues were collected from tumor parenchyma. Non-neoplastic brain tissues were collected from another 12 non-tumor subjects who received craniocerebral trauma infra-decompression and regarded as the control group. There were l0 males and 2 females and their ages ranged from 16 to 54 years. The experiment had got confirmed consent from local ethic committee and the collection was provided confirmed consent from patients and their relatives. All samples were restained with HE staining so as to diagnose as the brain glioma. While, all patients with brain glioma received radiotherapy after operation and their survival periods were followed up. METHODS： Primary lesion wax of brain glioma was cut into serial sections and stained with S-P immunohistochemical staining. Brown substance which was observed in tumor nucleus was regarded as the positive expr

关键词： brain glioma proliferating cell nuclear antigen P27 protein pathological grade prognosis

DELETION AND 5'CPG ISLAND METHYLATION OF p15 GENE IN brain glioma

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Chinese Journal of Cancer Research 2000年第2期12卷 152-154页

作者：翟广袁先厚祁晋清河南医科大学河南郑州 450052 湖北医科大学湖北武汉 430071

Objective: To investigate the abnormality of p15 gene in brain glioma and the correlation of it with occurrence or malignant progression of brain glioma. Methods: Deletion and 5'CPG island methylation of p15 gene were detected by the methods of PCR and PCR- based methylation in 56 cases of brain glioma. Results: Out of 43 cases of high grade glioma, 14 cases were found to have homozygous deletion of p15E1, while none of the 13 cases of low grade glioma was found to have deletion of p15E1 (Pglioma. Conclusion: Abnormality of p15 gene may involved in the occurrence and malignant progression of brain glioma. Homozygous deletion of gene is the major mechanism of inactivation for p15 gene in brain glioma.

关键词： p15 brain glioma Methylation PCR

AKT1 and AKT2 Promote Malignant Transformation in Human brain glioma LN229 Cells

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Clinical oncology and cancer researeh 2011年第3期8卷 144-148页

作者： Jian ZOU Kun WANG Lei HAN An-ling ZHANG Zhen-dong SHI Pei-yu PU Chun-sheng KANG Department of Neurosurgery Tianjin Medical University General Hospital Laboratory of Neuro-oncology Tianjin Neurological Institute Tianjin 300052 China

OBJECTIVE To confirm the role played by AKT1 and AKT2 in the β- catenin/Tcf-4 signaling pathway in promoting malignant transfor- mation of glioma cells. METHODS LN229 cells were divided into five groups： a control group, acetone （ACE）group, acetylsalicylic acid （ASA; aspirin） group, ASA＋AKT1 plasmid group and ASA＋AKT2 plasmid group. Western blot and PCR were used to detect the expression of AKT1 and AKT2 after dealing with ASA and transferring AKTI/2 genes into LN229 cells. Cell proliferation was determined by flow cytometry, cell invasion was evaluated by transwell assay and cell apoptosis was detected with annexin V staining. The molecules regulating proliferation and invasion were examined by western blot analysis. RESULTS Aspirin down-regulates AKT1 and AKT2 expression by modulating β-cateninfrcf-4 activity. AKT1 and AKT2 can enhance cell proliferation and invasion by up-regulating the expression of cyclin-D and matrix metalloprotein-9 （MMP-9） in LN229 glioma cells. CONCLUSION AKT1 and AKT2 play an important role in the β- catenin/Tcf-4 signaling pathway promoting malignant transformation; AKT1 is more effective than AKT2. AKT1 and AKT2 may be potential targets for brain glioma therapy and an effective way to prevent metastasis of gliomas.

关键词： AKT1 AKT2 brain glioma malignant transformation.

The use of a new functional glucose conjugate material, TPGS_(1000)-Glu, in treatment of brain glioma by incorporating into epirubicin liposomes

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Journal of Chinese Pharmaceutical Sciences 2016年第4期25卷 266-274页

作者：沐黎敏吴佳栓谢红军刘磊曾凡阎妍赵曜胡英杰卜英子吕万良北京大学医学部药学院天然药物与仿生药物国家重点实验室分子药剂学与新释药系统北京市重点实验室北京100191

Most of antieancer agents can not be used for treatment of brain glioma due to the existence of the blood brain barrier （BBB）. The over-expression of glucose transporters （GLUTs） on the BBB and brain glioma cells enables the possibility that the GLUTs ligand modified drug carrier transports across the BBB, and targets to the brain glioma cells. The objectives of the present study were to synthesize a new glucose conjugate material, TPGS1000-Glu, develop a kind of TPGSI00o-Glu modified epirubicin liposomes, and evaluate their efficacy. The studies were performed on the BBB co-culture model and brain glioma cells in vitro. TPGS 1000-Glu was synthesized by conjugating TPGSlo00_COOH with 4-aminophenyl-[3-D-glucopyranoside （Glu）, and confirmed by MALDI-TOF-MS spectrum. TPGS^0oo-GIu modified epirubicin liposomes were prepared with a high drug encapsulation efficiency （〉97%）, a nanosize （approximately 90 nm）, and a minimal drug leakage in fetal bovine serum （FBS）-containing buffer system. The BBB co-culture model was established, and after applying TPGSl0oo-Glu modified epirubicin liposomes to the model, transport of liposomal drug across the BBB was evidenced. Besides, TPGS1000-Glu modified epirubicin liposomes showed the strongest cellular drug uptake and anti-glioma efficacy after transport across the BBB in vitro. The synthesized TPGS1000-Glu material could offer a new targeting ligand for the BBB, while the developed TPGS1000-Glu modified epirubicin liposomes might provide a potential anticancer formulation for treatment of brain glioma.

关键词： TPGS1000-Glu Epirubicin Liposomes Glucose transporters brain glioma

Prediction of malignancy selective neural networks degree in brain glioma using ensemble

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Journal of Shanghai University(English Edition) 2006年第3期10卷 244-246页

作者：刘天羽李国正吴耿锋 School of Computer Engineering and Science Shanghai University Shanghai 200072 P.R. China

A clustering algorithm based selective neural networks ensemble （CLUSEN） is proposed to predict the degree of malignancy in brain glioma. Since the degree prediction of malignancy is critical before brain surgery, many learning methods are used like rule induction algorithm, single neural networks, support vector machines, etc. Ensemble learning methods can improve the generalization of single learning machine, and are becoming popular in the machine learning and medical data processing communities. The procedure of CLUSEN can efficiently remove redundancy learning individuals and help improve the diversity of ensemble methods. CLUSEN is used to predict the degree of malignancy in brain glioma. Experimental results on a set of brain glioma data show that, compared to support vector machines, rule induction and single neural networks, the classification accuracy of CLUSEN is higher.

关键词： ensemble learning neural networks brain glioma clustering algorithm.

Therapeutic Effects of Stereotactic Radiotherapy on 389 Cases of brain glioma

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The Chinese-German Journal of Clinical Oncology 2005年第4期4卷 221-224页

作者：牛道立何中胡慧玲广州医学院第一附属医院放疗科 510120

Objective： To investigate the treatment effectiveness and side effects of stereotactic radiotherapy for brain glioma. Methods： From Jun. 1995 to Dec. 1998, 389 cases of brain gliomas were treated by stereotactic radiotherapy, among which 151 cases were treated by stereotactic radiosurgery （SRS） and the other 238 cases, by fractionated stereotactic radiotherapy （FSRT）. In the SRS group, the marginal tumor dose was 20 to 30 Gy （median, 2.6 Gy）. One to 6 isocenters （median, 2.48） and 5 to 21 irradiation arcs （median, 8.45） were applied. In the FSRT group, the per-fraction marginal tumor dose was 8 to 12 Gy with 1 to 6 isocenters （median, 2.53）, 6 to 20 irradiation arcs （median, 8.25） and 2-5 fractions delivered everyday or every other day. Results： Three months after treatment, the complete and partial response rates were 13.9% and 45.7% in SRS group respectively. The stable disease rate was 17.2%. The total effective rate was 76.8%. In FSRT group, the complete and partial remission rates were 19.7% and 47.9% respectively. The stable disease rate was 20.6%. The total effective rate was 88.2%. The total effective rate of FSRT group was higher than that in SRS group （X^2=9.874, P=0.020）. The 1-year, 3-year and 5-year survival rate of all patients was 54.3%, 29.3%, 16.5% respectively. The 1-year, 3-year and 5-year survival rate in SRS group and FSRT group was 52.3% vs 26.5%, 11.9% vs 55.5%, and 31.1 vs 19.3% respectively. There was no significant difference between the two groups （X^2=2.16, P=0.1417）. The brain edema caused by the main radiation was more severe in the SRS group than in FSRT group （X^2=4.916, P=0.027）. Conclusion： It is effective for brain glioma to be treated by stereotactic radiotherapy. Compared with SRS, the FSRT has the advantage of good effect and less side response.

关键词： fractionated stereotactic radiotherapy stereotactic radiosurgery brain glioma prognosis

A Novel Core-Shell Structural Phospholipid-Functionalized Mesoporous Silica Nanoparticles Modified with Angiopep-2 as Targeting Drug Delivery System for brain glioma

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A Novel Core-Shell Structural Phospholipid-Functionalized Me...

IMMUNOHISTOCHEMICAL DETECTION OF P73 PRODUCTIN brain gliomaS

2014年中国药学大会暨中国药师周

作者： Guowei Wang Junjun Xu Manman Guo Rongrong Zhang Weidong Fei Fanzhu Li Department of Pharmaceutics Zhejiang Chinese Medical University

In order to improve the drug loading coefficient and realize the targeting delivey of paclitaxel（PTX）to brain and glioma,a novel core-shell structural phospholipid-functionalized mesoporous silica nanoparticles（MSN-LP）modified with Angiopep-2（ANG-MSN-LP）were successfully developed using selfassembly and film hydration method,and mesoporous silica nanoparticles（MSN）synthesized by modified-Stober method also as *** introducing the innovative preparation process of saturated solution adsorption method,PTX was highly encapsulated（drug loading efficiency up to 11.17%）into MSN（MSN-PTX）by forming crystal analogue complex inside the mesoporous pore *** of in vitro release showed that about 75.5%of PTX released from ANG-MSN-LP-PTX（PTX loaded into ANG-MSN-LP）after 50 h and burst release was effectively reduced compared with MSN-PTX or PTX solution,indicating pronounced sustained-release *** biological safety of ANG-MSN-LP was evaluated by HBMEC and C6 cells,which proved ANG-MSN-LP has good biocompatibility and low *** to the control PTX formulations of MSN-PTX,MSN-LP-PTX（PTX loaded into MSN-LP）and PTX solution,ANG-MSN-LP-PTX were proved to increase the transport ratio of the PTX across the BBB and afterwards target the brain glioma and displayed higher cell uptake,stronger inhibition and apoptosis toward glioma cells,better glioma curing effect,and improved MST of C6 cells-implanted ***,in pharmacokinetics by using blood and intracerebral microdialysis simultaneously,the AUC intracerebral of ANG-MSN-LP-PTX was increased to approximate 5.29-fold compared to that of PTX solution,but the AUCblood ***,the most surprising is that the concentration-time curve of injected ANG-MSN-LP-PTX and MSN-LP-PTX tended to be similar to oral administration,which indicating that the nanoparticles as drug reservoirs can be detained in *** conclusion,ANG-MSN-LP are prospective targeting drug delivery system for ther

关键词： Mesoporous silica nanoparticles Angiopep-2 Saturated solution adsorption method Blood-brain barrier brain glioma Blood and intracerebral microdialysis simultaneously

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Chinese Journal of Cancer Research 1999年第1期11卷 53-55页

作者：翟广袁先厚潘惠锦邱尚明周明勇湖北医科大学附属第二医院湖北武汉 Chun Shan Medical College 台湾

Objective: To elucidate the role of p73 in the genesisor development of glioma. Methods: P73 and p53expression of 63 gliomas were detected by immunohistochemistry. Results: Out of the 63 gliomas, 17 casesappeared p73 positive. The positive-rate in high gradegliomas was higher than that in low grade gliomas(x2=4.75, Pgliomas, 12 cases overexpressed p53 proteill. Conclusion:Overexpression of wild p73 may involve in the genesis ordevelopment of glioma.

关键词： p73 p53 brain glioma Immunohistochemistry.

The top cited articles on glioma stem cells in Web of Science

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Neural Regeneration Research 2013年第15期8卷 1431-1438页

作者： Fuxin Yi Jun Ma Weimin Ni Rui Chang Wenda Liu Xiubin Han Dongxiao Pan Xingbo Liu Jianwu Qiu Department of Neurosurgery First Affiliated Hospital of Liaoning Medical University

BACKGROUND： glioma is the most common intracranial tumor and has a poor patient prognosis. The presence of brain tumor stem cells was gradually being understood and recognized, which might be beneficial for the treatment of glioma. OBJECTIVE： To use bibliometric indexes to track study focuses on glioma stem cell, and to investigate the relationships among geographic origin, impact factors, and highly cited articles indexed in Web of Science. METHODS： A list of citation classics for glioma stem cells was generated by searching the database of Web of Science-Expanded using the terms ＂glioma stem cell＂ or ＂glioma, stem cell＇＂ or ＂brain tumor stem cell＂. The top 63 cited research articles which were cited more than 100 times were retrieved by reading the abstract or full text if needed. Each eligible article was reviewed for basic information on subject categories, country of origin, journals, authors, and source of journals. Inclusive criteria：（1） articles in the field of glioma stem cells which was cited more than 100 times; （2） fundamental research on humans or animals, clinical trials and case reports; （3） research article; （4） year of publication： 1899-2012; and （5） citation database： Science Citation Index-Expanded. Exclusive criteria：（1） articles needing to be manually searched or accessed only by telephone; （2） unpublished articles; and （3） reviews, conference proceedings, as well as corrected papers. RESULTS： Of 2 040 articles published, the 63 top-cited articles were published between 1992 and 2010. The number of citations ranged from 100 to 1 754, with a mean of 280 citations per article. These citation classics came from nineteen countries, of which 46 articles came from the United States. Duke University and University of California, San Francisco led the list of classics with seven papers each. The 63 top-cited articles were published in 28 journals, predominantly Cancer Research and Cancer Cell, followed by Cell Stem Cell and Nature. CONCLUSION： Our bib

关键词： Neural regeneration reviews brain glioma stem cells glioma stem cells cancer stem cells literature analysis Web of Science bibliometrics citation neuroregeneration