检索结果-南通市图书馆

Multi-walled carbon nanotubes reversing the bone formation of bone marrow stromal cells by activating M2 macrophage polarization

引用

Regenerative Biomaterials 2023年第1期10卷 980-989页

作者： Runlian Lin Kun Ge Dehui Fan Jing Li Guoqiang Zhou Kaihan Zhang Yuanyu Huang Lili Ma Jinchao Zhang Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education Key Laboratory of Chemical Biology of Hebei ProvinceCollege of Chemistry and Material ScienceHebei UniversityBaoding 071002China College of Basic Medical Science Hebei UniversityBaoding 071000China Department of Chemistry The University of ManchesterManchester M139PLUK School of Life Science School of Medical TechnologyAdvanced Research Institute of Multidisciplinary ScienceKey Laboratory of Molecular Medicine and BiotherapyKey Laboratory of Medical Molecule Science and Pharmaceutics EngineeringBeijing Institute of TechnologyBeijing 100081China

Multi-walled carbon nanotubes(MWCNTs)are an excellent bone tissue repair material both in vitro and in *** interactions between MWCNTs and single type of cells of bone tissue,including osteoblasts,bone marrow stromal cells(BMSCs)or osteoclasts,have been extensively ***,the interactions between MWCNTs with different types of cells in the bone microenvironment remain *** microenvironment is a complex system composed of different types of cells,which have interactions between each *** this work,the effects of MWCNTs on bone microenvironment were firstly studied by culture of MWCNTs with BMSCs,osteoblasts,osteoclasts,macrophages and vascular endothelial cells,***,co-culture systems of macrophages-BMSCs,macrophages-calvaria and macrophages-BMSCs-vascular endothelial cells were treated with MWCNTs,*** osteogenic differentiation of BMSCs and osteoblasts was inhibited when these two types of cells were cultured with MWCNTs,***,when co-culture MWCNTs with BMSCs and macrophages,the osteogenesis of BMSCs was promoted by inducing the M2 polymerization of ***,MWCNTs promoted the bone formation in the osteolysis model of calvaria ex *** addition,the formation of osteoclasts was inhibited,and angiogenesis was increased when treated with *** study revealed the inconsistent effects of MWCNTs on single type of bone cells and on the bone *** results provided basic research data for the application of MWCNTs in bone tissue repair.

关键词： multi-walled carbon nanotubes bone microenvironments osteogenesis bone marrow stromal cells M2 macrophages cellular interactions

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ytterbium ion promotes apoptosis of primary mouse bone marrow stromal cells

引用

Journal of Rare Earths 2015年第4期33卷 445-452页

作者：戴春燕陈士柱王朝张良葛昆张金超 Chemical Biology Key Laboratory of Hebei Province College of Chemistry & Environmental Science Hebei University Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education Hebei University Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education College of Chemistry & Chemical Engineering Hainan Normal University

One of the main target organs for the lanthanides （Ln） is bone. Previous studies revealed that ytterbium （Yb） produced damage to the skeletal system in vivo. But the effects of Yb3＋ on bone marrow stromal cells （BMSCs） in vitro had not been reported. In this paper, cell viability, apoptosis, mitochondrial membrane potential （MMP）, reactive oxygen species （ROS） and lactate dehy- drogenase （LDH） were measured in order to study the effects of Yb3＋ on BMSCs. The results indicated that Yb3＋ displayed a slight positive effect on the BMSCs viability at concentrations of 1 x 104, 1 × 105, and 1 x 104 mol/L, but turned to decrease the viability of BMSCs at the highest concentration of 1 × 103 mol/L for 24, 48 and 72 h. Yb3＋ at 1 x 103 mol/L promoted apoptosis of BMSCs, in- creased the levels of ROS and LDH, and decreased MMP in BMSCs. It suggested that the precipitate of YbPO4 might decrease the viability of BMSCs. Yb3＋ induced the apoptosis of BMSCs via mitochondrial pathway. The results might be useful for more rational application of Yb-based compounds in the future.

关键词： ytterbium bone marrow stromal cells precipitation apoptosis rare earths

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of La^(3+) on osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells

引用

Journal of Rare Earths 2012年第1期30卷 90-93页

作者： ZHANG Jinchao SUN Jing GU Guangqi HAO Xiaohong LIU Dandan LI Yaping QIN Xinying WANG Shuxiang Chemical Biology Key Laboratory of Hebei Province College of Chemistry and Environmental ScienceHebei UniversityBaoding 071002China Affiliated Hospi-tal of Hebei University Baoding 071000China

In order to elucidate the action of La3＋ on bone metabolism,effects of La3＋ on the osteogenic and adipogenic differentiation of pri-mary mouse bone marrow stromal cells（BMSCs） were studied by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTT） test,alkaline phosphatase（ALP） activity measurement,mineralized function,oil red O stain and *** results showed that La3＋ pro-moted the proliferation of BMSCs except at 1×10-10 and 1×10-6 mol/*** effect of La3＋ on the osteogenic differentiation depended on con-centrations at the 7th day,but the osteogenic differentiation was inhibited at any concentration at the 14th ***3＋ promoted the formation of mineralized matrix nodules except at 1×10-8 and 1×10-5 mol/***3＋ inhibited adipogenic differentiation except at 1×10-10 and 1×10-7 mol/L at the 10th day,and inhibited adipogenic differentiation except at 1×10-9 mol/L at the 16th *** findings suggested that La3＋ might have protective effect on bone at appropriate dose and *** would be valuable for better understanding the mechanism of the effect of La3＋ on bone metabolism.

关键词： La3+ bone marrow stromal cells osteogenic differentiation adipogenic differentiation mineralization rare earths

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Biocompatibility Studies on bone marrow stromal cells with Chitosan-gelatin Blends

引用

Journal of Wuhan University of Technology(Materials Science) 2004年第4期19卷 30-33页

作者：杨操 Huazhong University of Science and Technology

To study the effect of chitosan-gelatin blends on the growth and proliferation of in vitro cultured bone marrow stromal cells(BMSCs) and explore a new carrier for the application of tissure engineering, cells from long bones of young rabbitsaged less than two weeks were expanded in vitro for one week and seeded onto the surface of pure chitosan and chitosan-gelatin blends. cells attached to and proliferated on both pure chitosan and chitosan-gelatin blends were monitored with the aid of an inverted light microscope and a scanning electron microscope. The cell viability was monitored by MTT after 2, 4, 6, 8 days seeding. BMSCs could be attached to and proliferated on both pure chitosan and chitosan-gelatin blends and remain their morphologies seen in vivo. Chitosan-gelatin blends could promote BMSCs to proliferate(Pcells tissue engineering.

关键词： chitosan gelatin bone marrow stromal cells tissue engineering

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of bone marrow stromal cells and on the adipogenic trans-differentiation of osteoblasts

引用

Progress in Natural Science:Materials International 2005年第11期15卷 993-999页

作者： LI Xianghui, ZHANG Jinchao, SUI Senfang and YANG Mengsu (State Key Laboratory of Biomembrane, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China Department of Chemistry, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China) State Key Laboratory of Biomembrane Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China. Department of Chemistry College of Chemistry & Environmental Science Hebei University Baoding 071002 China. Department of Biology and Chemistry City University of Hong Kong Hong Kong China

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), alkaline phosphatase (ALP) activity and oil red O assays were used to examine the effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (MSCs) and the adipogenic trans-differentiation of primary mouse osteoblasts. The results indicated that daidzein, genistein and glycitein at concentrations from 1×10-8 mol/L to 1×10-5mol/L promoted the proliferation of MSCs and osteoblasts; genistein, daidzein and glycitein promoted osteogenic differentiation and inhibited adipogenic differentiation of MSCs, and inhibited adipocytic transdifferentiation of osteoblasts at appropriate concentrations as 17β-estradiol. It suggests that genistein, daidzein and glycitein regulate a dual differentiational process of MSCs into the osteogenic and adipogenic lineages, and trans-differentiational process of primary osteoblasts into the adipocyte lineages, causing a lineage shift toward osteoblast. Protective effects of them on bone may be mediated by a reversal of adipogenesis which may promote the proliferation, differentiation and mineralization of osteoblasts, and make adipocytes secrete less cytokines which may promote osteoclast formation and activation. In addition, the results also indicated that genistein, daidzein and glycitein may be helpful in preventing the development of steroid induced osteonecrosis.

关键词： genistein daidzein glycitein bone marrow stromal cells osteoblasts osteogenic differentiation adipogenic differentiation adipogenic trans-differentiation.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Role of Notch expression in premature senescence of murine bone marrow stromal cells

引用

Progress in Natural Science:Materials International 2009年第5期19卷 557-562页

作者： Kejie Zhanga,b, Lifang Huang b, Hanying Sunb, Yan Zhub, Yi Xiaob, Mei Huang b, Wenli Liub,a Department of Hematology, Zhongshan Hospital, Xiamen University, Fujian Medical University Clinic Teaching Hospital, Xiamen 361004, China b Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China [a]Department of Hematology Zhongshan Hospital Xiamen University Fujian Medical University Clinic Teaching Hospital Xiamen 361004 China [b]Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

The aim of the present study was to investigate the role of the Notch signaling pathway in premature senescence of murine bone marrow stromal cells in vitro. The intracellular domain of Notch 1 (ICN) was transfected into cultured murine bone marrow stromal cells by lipofectamine transfection. After three days, the proliferation of transfected cells was measured by MTT assay. Cell cycle distribution was analyzed by flow cytometry. Senescence-associated beta-galactosidase (SA-beta-gal) was measured, and the percentage of positive cells was evaluated by assessing 1000 cells in random fields of view. The expressions of p53 and p21Cip1/Waf1 were analyzed by both RT-PCR and Western blot analysis. The results showed that activation of Notch signaling inhibited proliferation of murine bone marrow stromal cells with induction of G1 arrest, increased the percentage of SA-beta-gal positive cells, and upregulated p53 and p21Cip1/Waf1 mRNA and protein expression levels. Thus, the activated Notch signaling could induce premature senescence of bone marrow stromal cells through the p53-p21Cip1/Waf1 pathway.

关键词： bone marrow stromal cells Premature senescence Notch signaling pathway

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Protective Effects and Mechanism of Panax Notoginseng Saponins on Oxidative Stress-Induced Damage and Apoptosis of Rabbit bone marrow stromal cells

引用

Chinese Journal of Integrative Medicine 2010年第6期16卷 525-530页

作者：强辉张晨时志斌杨华清王坤正 Department of Orthopaedics Shaanxi Provincial People's Hospital the Third Affiliated Hospital Medical School of Xi'an Jiaotong University Department of Orthopaedics the Second Affiliated HospitalMedical School of Xi'an Jiaotong University Department of Orthopaedics the Second Affiliated Hospital of Tsinghua University

Objective： To investigate the effects and possible mechanism of Panax Notoginseng saponins （PNS） on oxidative stress-induced damage and apoptosis in bone marrow stromal cells （BMSCs）.Methods： BMSCs were isolated and cultured from 2-month-old New Zealand rabbits by the density gradient centrifugation combined with adherent *** third passage cells were used for subsequent *** stress was induced in cultured BMSCs by H2O2 （0.1 mmol/L）.BMSCs were pretreated with 25-200 μg/mL PNS for 4 h before H2O2 *** of BMSCs was observed using MTT *** phosphatase （ALP） activity,as an index of early osteoblastic differentiation,was determined with an ALP assay *** cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/ propidium *** stress level was examined by reactive oxygen species （ROS） *** protein expressions of Bax,Bcl-2 and Caspase-3 in BMSCs were analyzed by Western ***： PNS had different concentrationdependent effects on proliferation and osteoblast differentiation of BMSCs induced by H2O2.A PNS concentration of 100 μg/mL was determined as the optimal effective *** markedly attenuated H2O2-induced apoptosis rate from 41.91% to 14.67% （P〈0.01）.PNS significantly decreased ROS level induced by H2O2 （P〈0.01）.Furthermore,pretreatment with PNS significantly reversed H2O2-induced inhibition of Bcl-2 expression and augmentation of Bax and Caspase-3 expression （P〈0.01）.Conclusion： PNS had a protective effect on oxidative stress-induced damage and apoptosis in cultured rabbit BMSCs through scavenging ROS and regulating the Bcl-2/Bax pathway.

关键词： Panax Notoginseng saponins oxidative stress bone marrow stromal cells apoptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Adipose-derived stromal cells resemble bone marrow stromal cells in hepatocyte differentiation potential in vitro and in vivo

引用

World Journal of Gastroenterology 2017年第38期23卷 6973-6982页

作者： Li-juan Xu Shu-fang Wang De-Qing Wang Lian-jun Ma Zheng Chen Qian-Qian Chen Jun Wang Li Yan Department of Gastroenterology Institute of Geriatrics Chinese PLA General Hospital Department of Blood Transfusion Chinese PLA General Hospital Department of Endoscopics China-Japan Union Hospital of Jilin University Department of Gastroenterology Institute of Surgery Research Daping Hospital Third Military Medical University

AIM To investigate whether mesenchymal stem cells(MSCs) from adipose-derived stromal cells(ADSCs) and bone marrow stromal cells(BMSCs) have similar hepatic differentiation *** Mouse ADSCs and BMSCs were isolated and cultured. Their morphological and phenotypic characteristics, as well as their multiple differentiation capacity were compared. A new culture system was established to induce ADSCs and BMSCs into functional hepatocytes. Reverse transcription polymerase chain reaction, Western blot, and immunofluorescence analyses were performed to identify the induced hepatocytelike cells. CM-Dil-labeled ADSCs and BMSCs were then transplanted into a mouse model of CCl4-induced acute liver failure. fluorescence microscopy was used to track the transplanted MSCs. Liver function was tested by an automatic biochemistry analyzer, and liver tissue histology was observed by hematoxylin and eosin(HE) *** ADSCs and BMSCs shared a similar morphology and multiple differentiation capacity, as well as a similar phenotype(with expression of CD29 and CD90 and no expression of CD11 b or CD45). Morphologically, ADSCs and BMSCs became round and epithelioid following hepatic induction. These two cell types differentiated into hepatocyte-like cells with similar expression of albumin, cytokeratin 18, cytokeratin 19, alpha fetoprotein, and cytochrome P450. fluorescence microscopy revealed that both ADSCs and BMSCs were observed in the mouse liver at different time points. Compared to the control group, both the function of the injured livers and HE staining showed significant improvement in the ADSC-and BMSC-transplanted mice. There was no significant difference between the two MSC *** ADSCs share a similar hepatic differentiation capacity and therapeutic effect with BMSCs in an acute liver failure model. ADSCs may represent an ideal seed cell type for cell transplantation or a bio-artificial liver support system.

关键词： Adipose-derived stromal cells bone marrow stromal cells Cell differentiation Hepatocyte differentiation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Osteogenic Potential of Cultured bone marrow stromal cells Transfected with Transforming Growth Factor β_1 Gene in vitro

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2001年第2期21卷 130-133页

作者：易诚青郑启新郭晓东刘勇 Department of Orthopedics Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022

To study the osteogenic potential of cultured bone marrow stromal cells transfected with transforming growth factor β 1 gene in vitro , cultured BMSCs were transfected with the complexes of pcDNA 3 TGF β 1 and Lipofectamine Reagent in vitro . The cell proliferation was detected by MTT method and the morphological features of transfected BMSCs was observed. ALP stains and PNP method were used to measure ALP activity. In addition, the collagen type Ⅰ propeptides and mineralized matrixes were examined by immunohistochemical staining and tetracycline fluorescence labeling respectively. The morphological and biological characters of the transfected BMSCs were similar to those of osteoblasts and the cell proliferation was promoted. The cell layer displayed strong positive reaction for ALP stains and immunohistochemical staining. ALP activity and collagen type Ⅰ expression increased remarkably after transfection. Mineralized matrixes formed earlier and more in transfected BMSCs as compared with control group. It is concluded that transfecting with TGF β 1 gene could promote the osteogenic potential of cultured BMSCs.

关键词： transforming growth factor β 1 gene transfection bone marrow stromal cells osteogenic potential

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Recent updates on the biological basis of heterogeneity in bone marrow stromal cells/ skeletal stem cells

引用

Biomaterials Translational 2022年第1期3卷 3-16页

作者： Deepika Arora Pamela Gehron Robey National Institute of Dental and Craniofacial Research National Institutes of HealthDepartment of Health and Human ServicesBethesdaMDUSA Biosystems and Biomaterials Division National Institute of Standards and TechnologyDepartment of CommerceGaithersburgMDUSA Department of Biotechnology School of Biological Engineering&Life SciencesShobhit Institute of Engineering&Technology(Deemed-to-be-University)MeerutIndia

Based on studies over the last several decades,the self-renewing skeletal lineages derived from bone marrow stroma could be an ideal source for skeletal tissue ***,the markers for osteogenic precursors;i.e.,bone marrow-derived skeletal stem cells(SSCs),in association with other cells of the marrow stroma(bone marrow stromal cells,BMSCs)and their heterogeneous nature both in vivo and in vitro remain to be *** review aims to highlight:i)the importance of distinguishing BMSCs/SSCs from other“mesenchymal stem/stromal cells”,and ii)factors that are responsible for their heterogeneity,and how these factors impact on the differentiation potential of SSCs towards *** prospective role of SSC enrichment,their expansion and its impact on SSC phenotype is *** has also been given to emerging single cell RNA sequencing approaches in scrutinizing the unique population of SSCs within the BMSC population,along with their committed *** the factors involved in heterogeneity may help researchers to improvise their strategies to isolate,characterize and adopt best culture practices and source identification to develop standard operating protocols for developing reproducible stem cells ***,more scientific understanding of the molecular basis of heterogeneity is warranted that may be obtained from the robust high-throughput functional transcriptomics of single cells or clonal populations.

关键词： bone marrow stromal cells clonal analysis heterogeneity single cell analysis skeletal stem cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：