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Resonance Rayleigh scattering study of the interaction of bleomycina_5 with nucleic acids and its analytical application

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Science China Chemistry 2007年第5期50卷 629-637页

作者： LIU JiangTao1,2, LIU ZhongFang1 & LIU ShaoPu1 1 School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China 2 Department of Chemistry, Fuling Normal University, Chongqing 408000, China School of Chemistry and Chemical Engineering Southwest University Chongqing China Department of Chemistry Fuling Normal University Chongqing China

The interaction of bleomycina5 with nucleic acids has been investigated by using resonance Rayleigh scattering (RRS), molecular absorption and fluorescence spectra. The result shows that in near pH 2.2 buffer medium and absence of any metal ions, nucleic acids are capable of binding with bleomycina5 (BLMA5) to form complexes which can remarkably enhance the RRS intensity and result in batho- chromic and hyperchromic molecular absorption of nucleic acids and fluorescence quenching of bleomycina5. The RRS spectral characteristics for the binding products of bleomycina5 with various DNA and RNA are similar, and the maximum RRS peaks are at 301 nm for ctDNA and sDNA, 370 nm for hsDNA, 310 nm for RNAtypeVI and RNAtypeIII, respectively. The increments of RRS intensity are greatly different in which DNA enhances greatly and RNA enhances lightly. In this work, the optimum condi- tions of the interaction and some influencing factors have been investigated. The reaction mechanism and a binding model for the interaction of BLMA5 with the nucleic acids are discussed. In addition, a highly sensitive, simple and rapid new method for the determination of DNA has been developed. The detection limits (3σ) are 5.7 ng/mL for ctDNA, 7.4 ng/mL for sDNA and 9.2 ng/mL for hsDNA, respectively. The method can be applied to determination of trace amounts of DNA.

关键词： bleomycina5, nucleic acids, resonance Rayleigh scattering, fluorescence quenching, absorption spectrum.

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