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Phase-shift presaturation for water peak suppression in biomolecular NMR experiments

Science China(Life Sciences) 1997年第4期40卷 345-350页

作者：毛希安叶朝辉 State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics Chinese Academy of Sciences Wuhan 430071 China

The phase-shift presaturation technique for water suppression is further improved with effective phase cycling. In the two-dimensional NMR COSY, NOESY and HMQC experiments of biosamples, the water sup-pression factor reaches the order of 10 , making it possible to detect 13C NMR signals in extremely diluted (2 mmol/L) aqueous solutions.

关键词： biomolecular NMR water suppression phase-shift presaturation phase cycling.

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Raman microspectroscopic study of biomolecular structure inside living adhesive cells

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Science China(Life Sciences) 2002年第4期45卷 397-405页

作者：李光张志义杨红英许以明 Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

Cells adhesion is very important for many physiological processes. Using advanced Raman microspectroscopic technique, we selected T Leukemia cells (Jurkat) as the materials and obtained simultaneously conformation information of various biomolecules inside the whole living cells. By comparing the Raman microspectroscopic spectra of single and adhesive cancer cells, we found for the first time that when cells adhered, the conformation of the biomolecules (DNA, protein, carbohydrates and lipids) inside the cells had different changes: (i) the backbone of double-stranded DNA maintained orderly B-form or modified B-form conformation, whereas the groups of its deoxyribose and bases were modified; (ii) the conformational changes of the main chain and the side chain in the protein were obviously variant. The lines intensity belonging to α-helix andβ-sheet decreased, while that ofβ-turn increased. Tyrosine and tryptophane residues of the protein changed from "buried state" to "exposed state"; the lines intensity of its sulfhydryl group also increased; the conformation of its disulfide bond changed from two kinds to three kinds. These facts suggest that the cells adhesion causes changes in H-bonds organization of the main chain and environment of the side chain in the protein; (iii) the groups of the carbohydrates were also modified simultaneously; (iv) the conformation of the lipids bilayers of the membranes changed obviously; the order parameter for lateral interaction between chains decreased gradually with the increase of number of the adhesive cells. So cells adhesion resulted in an increase in fluidity of the membrane and ion permeability on the membrane.

关键词： Raman microspectroscopy, living cells, biomolecular structure, cell adhesion.

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The identities and anti-herpes simplex virus activity of Clinacanthus nutans and Clinacanthus siamensis

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Asian Pacific Journal of Tropical Biomedicine 2013年第4期3卷 284-290页

作者： Paween Kunsorn Nijsiri Ruangrungsi Vimolmas Lipipun Ariya Khanboon Kanchana Rungsihirunrat College of Public Health Sciences Chulalongkorn UniversityBangkok 10330Thailand Faculty of Pharmaceutical Sciences Chulalongkorn UniversityBangkok 10330Thailand

Objective:To distinguish the difference among the Clinacanthus nutans(Burm.f.)Lindau(***)and Clinacanthus siamensis Bremek(***)by assessing pharmacognosy characteristics,molecular aspect and also to evaluate their anti-herpes simplex virus(HSV)type 1 and type 2 ***:Macroscopic and microscopic evaluation were performed according to WHO Geneva *** number,stomatal index and palisade ratio of leaves were *** DNA was extracted by modified CTAB method and ITS region was amplified using PGR and then *** leaves were subsequently extracted with n-hexane,dichloromethane and methanol and antiviral activity was performed using plaque reduction assay and the cytotoxicity of the extracts on Vero cells was determined by MTT ***:Cross section of midrib and stem showed similar major *** measurement index of stomatal number,stomatal index and palisade ratio of *** were 168.32±29.49,13.83±0.86 and 6.84±0.66,respectively,while *** were 161.60±18.04,11.93±0.81and 3.37±0.31,*** PCR amplification of ITS region generated the PGR product approximately 700 bp in *** were 34 polymorphisms within the ITS region which consisted of 11 Indels and 23 nucleotide *** IC_(50)values of *** extracted with n-hexane,dichloromethane and methanol against HSV-1 were(32.05±3.63)μg/mL,(44.50±2.66)μg/mL,(64.93±7.00)μg/mL,respectively where as those of *** were(60.00±11.61)μg/mL,(55.69+4.41)μg/mL,(37.39±5.85)μg/mL,*** HSV-2 activity of n-hexane,dichloromethane and methanol *** leaves extracts were(72.62±12.60)μg/mL,(65.19±21.45)μg/mL,(65.13±2.22)μg/mL,respectively where as those of *** were(46.52±4.08)μg/mL,(49.63±2.59)μg/mL,(72.64±6.52)μg/mL,***:The combination of macroscopic,microscopic and biomolecular method are able to authenticate these closely related plants and both of them have a potency to be an anti-HSV age

关键词： Clinacanthus nutans Clinacanthus siamensis Microscopic analysis biomolecular analysis Internal transcribed spacer Herpes simplex virus Plaque reduction assay

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