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Time-gated fluorescence imaging:Advances in technology and biological applications

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Journal of Innovative Optical Health Sciences 2020年第3期3卷 12-31页

作者： Wenzhao Yang Sung-Liang Chen University of Michigan-Shanghai Jiao Tong University Joint Institute Shanghai Jiao Tong University Shanghai 200240 P.R.China State Key Laboratory of Advanced Optical Communication Systems and Networks Shanghai Jiao Tong University Shanghai 200240 P.R.China

Time-gated(TG)fluorescence imaging(TGFI)has attracted increasing attention within the biological imaging community,especially during the past *** rapid development of light sources,image devices,and a variety of approaches for TG implementation,TGFI has demonstrated numerous biological applications ranging from molecules to *** paper presents inclusive TG implementation mainly based on optical choppers and electronic units for synchronization of fluorescence excitation and emission,which also serves as guidelines for researchers to build suited TGFI systems for selected *** that a special focus will be put on TG implementation based on optical choppers for TGFI of long-lived probes(lifetime range from microseconds to milliseconds).biological applications by TG imaging of recently developed luminescent probes are described.

关键词： Time-gated fluorescence imaging system biological application

Photo-crosslinkable hydrogel and its biological applications

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Chinese Chemical Letters 2021年第5期32卷 1603-1614页

作者： Yuting Wang Shu Zhang Jian Wang State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of SromatologySichuan UniversityChengdu 610041China West China School of Stomatology Sichuan UniversityChengdu 610041China

In the past few years,photo-crosslinkable hydrogels have drawn a great attention in tissue engineering applications due to their high biocompatibility and extracellular matrix(ECM)-like *** can be easily biofabricated through exposure of a photosensitive system composed of photo-crosslinkable hydrogels,photo-initiators and other compounds such as cells and therapeutic molecules,to ultraviolet or visible *** the development ofbiofabrication methods,many researchers studied the biological applications of photo-crosslinkable hydrogels in tissue engineering,such as vascular,wound dressing and bone *** review highlights the biomaterials for photo-crosslinkable hydrogels,biofabrication techniques and their biological applications in tissue ***,the challenges and prospects of photo-crosslinkable hydrogels are discussed as well.

关键词： Photo-crossli nkable Biomaterial Hydrogel Biofabrication biological application

Fe3O4 Modified Up-Conversion Luminescent Nanocrystals for biological applications

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Chinese Journal of Chemistry 2012年第12期30卷 2774-2778页

作者： Hou Yu Yin Zifei Xin Hailiang Su Yonghua Yang Huagui Key Laboratory for Ultrafine Materials of Ministry of Education School of Materials Science and Engineering East China University of Science and Technology Shanghai 200237 China Department of Traditional Chinese Medicine Changhai Hospital The Second Military Medical University Shanghai 200433 China

Magnetic-luminescent nanocomposites have obtained a great deal of attention due to their potential applications in magnetic separation of cells, magnetic resonance imaging and luminescence imaging, and so on. Herein, a new and facile two-step synthetic strategy was developed to prepare magnetic Fe304 modified single crystalline up- conversion luminescent NaYF4： Yb3＋, Er3＋ nanocrystals using Fe304 nanoparticles as the seeds. Acting as the ＂nu- clei＂ in the reaction, the presence of Fe304 nanoparticles facilitates the formation of up-conversion NaYF4： Yb3＋, Er3＋ nanocrystals on their surfaces through heterogeneous nucleation process. The structure and properties of these multifunctional nanocrystals were characterized by transmission electron microscope （TEM）, X-ray photoelectron spectroscopy （XPS）, photo luminescence （PL） and X-ray diffraction （XRD）. The results revealed that the as-prepared multifunctional nanocrystals show dual distinct properties of up-conversion fluorescent and superpara- magnetism, which were cubic in shape and very uniform in size with an average size ranging 30m40 nm. These unique functionalities could be very attractive for biological systems, and some biological applications such as cell- labelling were also demonstrated.

关键词： up-conversion luminescent Fe3O4 modified heterogeneous nucleation biological application

Porphyrinic metal-organic frameworks for biological applications

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Advanced Sensor and Energy Materials

Advanced Sensor and Energy Materials 2023年第1期2卷 35-53页

作者： Li-Jian Chen Xu Zhao Xiu-Ping Yan State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122PR China International Joint Laboratory on Food Safety Jiangnan UniversityWuxi 214122PR China Institute of Analytical Food Safety School of Food Science and TechnologyJiangnan UniversityWuxi 214122PR China Key Laboratory of Synthetic and biological Colloids Ministry of EducationJiangnan UniversityWuxi 214122PR China

Porphyrins and their derivatives have been extensively applied in various fields owing to their photophysical and electrochemical ***,the drawbacks of self-aggregation and self-quenching in aqueous media limit their biological *** metal-organic frameworks(PMOFs)have attracted considerable attention because the introduction of porphyrins as organic linker into frameworks overcomes the limitations of free *** review summarizes the strategies for the construction of PMOFs and their biological *** challenges and chances displayed by this class of emerging materials are also discussed.

关键词： Porphyrinic metal-organic frameworks In situ synthesis Post-synthetic modification biological application

Phase transferring luminescent gold nanoclusters via single-stranded DNA

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Science China Chemistry 2022年第6期65卷 1212-1220页

作者： Yu Li Hui Lu Zhibei Qu Mingqiang Li Haoran Zheng Peilin Gu Jiye Shi Jiang Li Qian Li Lihua Wang Jing Chen Chunhai Fan Jianlei Shen Division of Physical Biology CAS Key Laboratory of Interfacial Physics and TechnologyShanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai201800China University of Chinese Academy of Sciences Beijing100049China School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative Molecules and National Center for Translational MedicineShanghai Jiao Tong UniversityShanghai200240China Shanghai Synchrotron Radiation Facility Zhangjiang LaboratoryShanghai Advanced Research InstituteChinese Academy of SciencesShanghai201210China Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular EngineeringEast China Normal UniversityShanghai200127China

Atomically precise gold nanoclusters(Au NCs) are an emerging class of quantum-sized nanomaterials with discrete electronic energy levels, which has led to a range of attractive electronic and optical applications. Nevertheless, the lack of general methods to transfer Au NCs protected with hydrophobic ligands to an aqueous solution hampers their use in physiological settings. Here,we developed a single-stranded DNA-based approach that could transfer ~90% hydrophobic Au NCs into an aqueous *** experimentally and theoretically established that multivalent electrostatic and hydrophobic interactions between DNA strands and the hydrophobic ligand layer on Au NCs resulted in monodispersed DNA-coated Au NCs with high physical integrity in an aqueous solution. The fluorescence quantum yield of Au NCs was increased by ~13 fold, and surface-constrained DNA retained the specific recognition ability for biosensing. We further demonstrated the versatility of this phase-transfer approach, which thus holds great potential to advance biological and medical applications of Au NCs.

关键词： gold nanoclusters DNA monodispersed phase transfer biological application

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Mono-functionalized pillar[n]arenes: Syntheses, host–guest properties and applications

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Chinese Chemical Letters 2024年第1期35卷 16-30页

作者： Wenzhi Yang Wenjie Zhang Jingyu Chen Jiong Zhou Department of Chemistry College of SciencesNortheastern UniversityShenyang 110819China

Pillar[n]arenes are a novel class of macrocyclic hosts reported by Ogoshi and co-workers in 2008. Because of their rigid pillar structures, interesting host-guest properties and ease of modifications, pillar[n]arenes have been developed rapidly in the field of functional materials and biomedicine. The modifications of pillar[n]arenes at different positions can give them varied characteristics. Functional groups can be introduced into one position of pillar[n]arenes without changing host-guest properties of pillar[n]arenes. A series of pillar[n]arene dimers, trimers, tetramers and metallacycles can be constructed by mono-functionalized pillar[n]arenes. In this review, two synthetic methods of mono-functionalized pillar[n]arenes are summarized and structures containing mono-functionalized pillar[n]arenes are described. Furthermore, the applications of mono-functionalized pillar[n]arenes in different fields (e.g., supramolecular polymers, sensors, molecular machines, catalysis, biological applications and light-harvesting systems) are also introduced. Hopefully, this article will be useful for researchers studying pillar[n]arenes, especially the mono-functionalized pillar[n]arenes.

关键词： Pillar[n]arenes Host-guest properties Supramolecular polymer Molecular machines biological application

Rim-differentiated pillar[5]arenes

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Chinese Chemical Letters 2021年第11期32卷 3322-3330页

作者： Lintao Wu Chun Han Xiaobi Jing Yong Yao Department of Chemistry Changzhi UniversityChangzhi 046011 China School of Chemistry and Chemical Engineering Nantong UniversityNantong 226019China School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225009China

Pillar[5]arenes,designed and prepared by Ogoshi et *** 2008 initially,refer to fifth classical *** a wide range of pillar[5]arenes,rim-differentiated pillar[5]arenes containing five identical substituents on one rim and five different identical groups on the other rims are considered the most noteworthy type of pillar[5]*** compared with the perfunctionalized pillar[5]arene,the self-assembly properties of rim-differentiated pillar[5]arenes have more *** the other hand,in comparison with other types of pillar[5]arenes,the rim-differentiated pillar[5]arenes exhibit a more rigid symmetrical *** the present review,the synthetic methods,host-guest interactions,self-assembly properties and applications of rim-differentiated pillar[5]arenes are ***,this review will be conducive to researchers in macrocyclic supramolecular chemistry.

关键词： Pillararene Rim-differentiated Self-assembly biological application Controlled release

Multifunctional Clay/PNIPAM Hydrogel Incorporating HxMoO3 Plasmonic Quantum Dot

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Energy & Environmental Materials 2020年第2期3卷 192-201页

作者： Hao Li Youzeng Li Qun Xu Ali Zhang College of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450052China School of Materials Science and Engineering Dalian University of TechnologyDalian 116000China

Functional hydrogel is becoming a frequently used material in various fields,especially in biological and medical *** order to overcome the barriers of low uniformity of structure and lack of energy dissipation effect in common hydrogel,in this work a strategy of doping plasmonic HxMoO3 quantum dots into PNIPAM(poly(N-isopropylacrylamide)) hydrogel to proceed its multifunctionalization is *** quantum dots-induced tactic could effectuate the enhancement of photothermal conversion,mechanical property,adhesion,and self-healing performance *** particular,for adhesion performance,the toughness value could be elevated to over 2500 J m-2 ***,the enhancement mechanism behind the extraordinary adhesion performance is studied,and it can be contributed to the synergistic effect of pore structure regulation and abundant hydrogen bond,which are both beneficial to the interaction between composite hydrogel and solid ***,based on its extraordinary adhesion and self-healing performance,the applicability of HxMoO3/PNIPAM hydrogel as a M? growth substrate is investigated on wound dressing,and the experimental result demonstrates its excellent M? growth-promoting activity.

关键词： biological application multifunctionalization plasmonic quantum dots ultraadhesive hydrogel

Synthesis and application of highly sensitive fluorescent probe for Hg2＋ regulated by sulfur

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Chinese Chemical Letters 2017年第10期28卷 2014-2018页

作者： Siyue Ma Linyang Li Mengyao She Yan Mo Shengyong Zhang Ping Liu Jianli Li Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry College of Chemistry & Materials Science Northwest University Xi'an 710127 China

Rhodamine-based fluorescent probe is widely used in chemical analysis, environmental analysis and life sciences area due to their excellent optical properties. Based on the thiophilic property of Hg2＋, using C = S structural motif as the core segment, our group have designed and synthesized three novel probes containing cinnamyl aldehyde with different substituents, exhibiting high selectivity and excellent sensitivity. The structure-property relationships of these probes have been investigated that the optical change caused by electron withdrawing effect and heavy atom effect. Furthermore, these Hg2＋ probes could be applied in living mice imaging, which provide a promising tool for quantitative mercury（Ⅱ） ion imaging in living organism.

关键词： Fluorescent probe Rhodamine 6G Mercury(Ⅱ) ion Electronic effect biological application