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Epigenetic Repression of Male Gametophyte- Specific Genes in the Arabidopsis Sporophyte

Molecular Plant 2013年第4期6卷 1176-1186页

作者： Robert D. Hoffmann Michael G. Palmgren Centre for Membrane Pumps in Cells and Disease--PUMPKIN Danish National Research Foundation Department of Plant and Environmental SciencesUniversity of Copenhagen Thorvaldsensvej 40 DK-1871 Frederiksberg Denmark

Tissue formation, the identity of cells, and the functions they fulfill, are results of gene regulation. The male gametophyte of plants, pollen, is outstanding in this respect as several hundred genes expressed in pollen are not expressed in the sporophyte. How pollen-specific genes are down-regulated in the sporophyte has yet to be established. In this study, we have performed a bioinformatics.analysis of publicly available genome-wide epigenetics data of sev- eral sporophytic tissues. By combining this analysis with DNase ! footprinting data, we assessed means by which the repression of pollen-specific genes in the Arabidopsis sporophyte is conferred. Our findings show that, in seedlings, the majority of pollen-specific genes are associated with histone-3 marked by mono- or trimethylation of Lys-27 （H3K27me1/ H3K27me3）, both of which are repressive markers for gene expression in the sporophyte. Analysis of DNase footprint profiles of pollen-specific genes in the sporophyte displayed closed chromatin proximal to the start codon. We describe a model of two-staged gene regulation in which a lack of nucleosome-free regions in promoters and histone modifications in open reading frames repress pollen-specific genes in the sporophyte.

关键词： male gametophyte epigenetics gene repression DNase I footprinting transcription factors bioinformatics.

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bioinformatics

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丛书名： Oxford higher education

2007年

作者： Bosu Orpita.

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bioinformatics. A Biologist's Guide to Biocomputing and the Internet 1

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2000年

作者： Stuart M. Brown

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Analysis on Sequence and bioinformatics.of Gene Fragment from Cassava

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Agricultural Biotechnology 2013年第1期2卷 16-17,32页

作者： Ruixia YAO Management Division of Facility and Asset Zhongkai University of Agriculture and Engineering

[ Objective ] The study aimed to establish an efficient method to extract RNA from cassava, and clone the core sequence d SSS II gene. [ Method ] The cassava RNA was obtained using the modified CTAB method, which was then reversely transefiptod into eDNA. Degenerate primers were designed based on the ho- mology property of known SSS II sequences in other plant species. A fragment was amplified with the previously mentioned eDNA as template and the degenerate primers through Polymerase Chain Reaction （PCR）. [Result] After online blasting in NCBI, the sequence was identified as the core fragment of cassava SSS I1 gene. [ Conclusion] Our research would lay the original basis for the cloning of the cassava SSS II full length cDNA sequence and construction of its anti - sense vector, which could further provide proper candidate genes for the development of starch metah）lic en^necring.

关键词： Cassava Soluble starch synthase Sequence bioinformatics.

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