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Effect of irrigation schedule on growth and seed yield of camelina(Camelina sativa L.)in Tarai region of central Himalaya

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Oil crop Science 2021年第1期6卷 8-11页

作者： Ankur Agarwal Om Prakash Madhu Bala Defence Institute of Bio-Energy Research Defence R&D OrganizationGoraparaoP.OArjunpurHaldwaniUttarakhand263139India

Camelina(Camelina sativa)has emerged as a potential biofuel crop globally with its suitability even as a jet fuel source with 75–80%less greenhouse gas emissions compared to common petroleum *** crop has originated from Mediterranean region and belongs to Brassicaceae ***,DRDO has made initial and pioneer efforts in successful introduction of this crop to India and its agro-technology *** a short duration crop with lesser input requirement,it fits well in the cropping pattern of hilly states of Indian *** areas experience fallow land due to shortage of irrigation water for growing other *** present study revealed that irrigation at flowering stage is more *** at this stage(if only one irrigation is available)exhibited grain yield of 1.004 kg·m^(-2)which was 21 and 80%higher over pod setting and rosette stage irrigation,*** maximum seed yield was observed under treatment where irrigation was provided at all three crop growth stages(2.044 kg·m^(-2)).It was 50%higher over T6 treatment(where two irrigations were given at rosette and flowering stage)and 104%higher over T3(where only one irrigation was given at flowering stage).

关键词： Irrigation scheduling Water requirement Water deficit biofuel crop False flax

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Functional characterization of caffeic acid O-methyltransferase in internode lignification of switchgrass(Panicum virgatum)

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Frontiers of Agricultural Science and Engineering 2018年第1期5卷 98-107页

作者： Fengyan WU Zhenying WU Aiguo YANG Shanshan JIANG Zeng-Yu WANG Chunxiang FU Key Laboratory of Energy Genetics of Shandong Province/Qingdao Engineering Research Center of Biomass Resources and Environment/Key Laboratory of biofuels/Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Tobacco Research Institute Chinese Academy of Agricultural Sciences Forage Improvement Division The Samuel Roberts Noble Foundation

Caffeic acid O-methyltransferase(COMT) is a crucial enzyme that mainly methylates phenylpropanoid meta-hydroxyl of C5 in the biosynthesis of syringyl lignin in angiosperms. A putative COMT, named as PvCOMT1,was isolated from switchgrass(Panicum virgatum), a C4 warm-season dual-purpose forage and bioenergy crop. Our results showed that recombinant PvCOMT1 enzyme protein catalyzed the methylation of 5-OH coniferyl alcohol, 5-OH coniferaldehyde(CAld5H) and 5-OH ferulic acid. Further in vitro studies indicate that CAld5H can dominate COMT-mediated reactions by inhibiting the methylation of the other substrates. Transgenic switchgrass plants generated by an RNAi approach were further employed to study the function of COMT in internode lignification. A dramatic decrease in syringyl lignin units coupled with an obvious incorporation in 5-OH guaiacyl lignin units were observed in the COMT-RNAi transgenic plants. However, the constitutive suppression of COMT in switchgrass plants altered neither the pattern of lignin deposition along the stem nor the anatomical structure of internodes. Consistent with the biochemical characterization of PvCOMT1, a significant decrease in sinapaldehyde was found in the COMT-RNAi transgenic switchgrass plants, suggesting that CAld5H could be the optimal intermediate in the biosynthesis syringyl lignin.

关键词： biofuel crop caffeic acid O-methyltransferase forage lignin Panicum virgatum switchgrass transgenic plant

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