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Journal of Environmental Sciences 2007年第6期19卷 667-673页

作者： ZHANG Zhi-qiang LIN Bo XIA Si-qing WANG Xue-jiang YANG A-ming State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and Engineering Tongji University Shanghai 200092 China College of Environmental -Science and Engineering Nanchang University Nanchang 330031 China

The flocculating activity of a novel bioflocculant MMF1 produced by multiple-microorganism consortia MM1 was investigated. MM1 was composed of strain BAFRT4 identified as Staphylococcus sp. and strain CYGS1 identified as Pseudomonas sp. The flocculating activity of MMF1 isolated from the screening medium was 82.9%, which is remarkably higher than that of the bioflocculant produced by either of the strains under the same condition. Brewery wastewater was also used as the carbon source for MM1, and the cost-effective production medium for MM1 mainly comprised 1.0 L brewery water （chemical oxygen demand （COD） 5000 mg/L）, 0.5 g/L urea, 0.5 g/L yeast extract, and 0.2 g/L （NH4）2SO4. The optimal conditions for the production of MMF1 was inoculum size 2%, initial pH 6.0, cultivating temperature 30℃, and shaking speed 160 r/min, under which the flocculating activity of the MMF1 reached 96.8%. Fifteen grams of purified bioflocculant could be recovered from 1.0 L of fermentation broth. MMF1 was identified as a macromolecular substance containing both protein and polysaccharide. It showed good flocculating performance in treating indigotin printing and dyeing wastewater, and the maximal removal efficiencies of COD and chroma were 79.2% and 86.5%, respectively.

关键词： bioflocculant multiple-microorganism consortia brewery wastewater carbon source indigotin printing and dyeing wastewater

Screening and Characterization of a bioflocculant Produced by Aeromonas sp

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Biomedical and Environmental Sciences 2007年第4期20卷 274-278页

作者： JIN-JING LIU XIAO-MING LI QI YANG KAI HUANG GUANG-MING ZENG DE-XIANG LIAO WEN-FANG LONG College of Environmental Science and Engineering Hunan University Changsha 410082 Hunan China

To isolate the bioflocculant-producing bacteria from activated sludge and investigate the flocculating characteristics of the newly isolated bioflocculant. Methods Bacteria were screened from activated sludge samples to isolate bioflocculant-producing bacteria. Flocculating activity was used as a measure of the flocculating capability of the bioflocculant. Results A novel bioflocculant-producing bacterium was isolated, which was identified to belong to genus Aeromonas and named as Aeromonas sp. Nil. Flocculating activity increased in the presence of K, Na^＋, or Ca^2＋. The highest flocculating activities for kaolin suspension were obtained in acidic pH ranges, and optimum pHs for it were 3.0, 4.0, and 5.0 with 1 mmol/L K^＋, Ca^＋, and Na^＋ present, respectively. The highest flocculating activities for soil suspension were observed at pH 8.0. The bioflocculant had a good flocculating activity and could achieve a flocculating activity of 92.4% for kaolin suspension at a dosage of only 1 mg.L-1, and its activity in kaolin suspension was decreased by only 9.2% after heating at 100℃ for 60 min. Conclusion The bioflocculant produced by Aeromonas sp. Nil has strong flocculating activity and high stability, which affords high possibility of its practical use.

关键词： Aeromonas sp. bioflocculant Biopolymer Flocculation

Characteristics and Culture Conditions of a bioflocculant Produced by Penicillium sp.

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Biomedical and Environmental Sciences 2010年第3期23卷 213-218页

作者： LI-FAN LIU WEN CHENG Faculty of Civil and Transportation Engineering Guangdong University of Technology Guangzhou 510006 Guangdong China Chemistry & Environment South China Normal University Guangzhou 510631 Guangdong China

Objective To study the characteristics of a bioflocculant named MBF7 produced by Penicillum strain HHE-P7 and the effects of cultivation conditions on bioflocculant production. Methods The chemical group in the bioflocculant molecules was shown by Fourier transform infrared （FTIR） spectra, and the average molecular weight of MBF7 was estimated by gel permeation chromatography. The effects of medium components on bioflocculant production and flocculating activity were studied. Results Phospho-, amino-, hydroxyl, and carboxyl groups were the major fractions of MBF7, and the molecule weight was about 3.0 × 10^5 Da. In addition, the carbon and nitrogen sources favorable for the bioflocculant production were glucose and yeast extract respectively. When the initial pH of the medium was adjusted to 5.0, high flocculant efficiency could be achieved. Conclusion The bioflocculant MBF7 is a new macromolecule with high flocculating efficiency for Kaolin suspension, and could be produced under appropriate culture conditions.

关键词： bioflocculant Penicillium sp. Flocculating activity Carbon and nitrogen sources

Studies on bioflocculant Production by Pseudoalteromonas sp. NUM8, a Marine Bacteria Isolated from the Circulating Seawater

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Journal of Ocean University of China 2021年第5期20卷 1276-1284页

作者： FU Wandong LIAO Miaofei ZHANG Dongxu ZHOU Yufang YANG Huicheng Zhejiang Marine Development Research Institute Zhoushan 316021China Marine Fishery Institute of Zhejiang Province Key Laboratory of Mariculture&Enhancement of Zhejiang ProvinceZhoushan 316021China

A bioflocculant producing potential bacteria was isolated from the circulating seawater of bio-filter using streak plate methods.The bacteria was identified through biochemical characteristics,partial 16S ribosomal ribonucleic acids(rRNA),nucleo-tide sequencing,and BLAST analysis of the gene sequence that showed the bacteria have 99%similarity to Pseudoalteromonas sp.and deposited in GenBank as Pseudoalteromonas sp.NUM8 with accession number JX435820.Influences of time course assay,carbon sources,nitrogen sources,inoculum size,as well as initial pH on the bacteria producing extracellular bioflocculant activity were investigated.The results showed that the strain optimal production period of microbial bioflocculant was at 72 h(flocculating activity of 94.5%),then dropped slowly.The bacteria optimally produced the bioflocculant when 1.0%sucrose and 0.5%sodium nitrate were used as sole sources of carbon and nitrogen with flocculating activities of 92.8%and 93.8%respectively.Also,the bacteria produced the bioflocculant optimally when initial pH of the medium was 5.0(flocculating activity 93.2%),and when Ca^(2+)was used as cation(flocculating activity 93.4%).The culture condition of inoculum size of 3%(v/v)was optimal flocculant pro-duction(flocculating activity 94.4%).Composition analyses indicated the bioflocculant to be principally a glycoprotein made up of about 34.3%protein and 63.4%total carbohydrate.

关键词： Pseudomonas sp.NUM8 bioflocculant flocculating activity circulating seawater

Screening and flocculating properties of bioflocculant-producing microorganisms

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Journal of University of Science and Technology Beijing 2006年第4期13卷 289-292页

作者： Yanling Sheng Qiang Zhang Yanru Sheng Chengbin Li and Huajun Wang Civil and Environmental Engineering School University of Science and Technology Beijing Beijing 100083 China Nanguan District Teacher Advanced Study School of Changchun Changchun 130043 China Guangdong Institute of Microbiology Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application Guangzhou 510070 China

Screening of bioflocculant-producing microorganisms was carded out. A strain that secreted excellent bioflocculant was isolated from municipal sewage using the spread plate technique, identified as Klebsiella sp. by the analytical profile index （API） identification system, and named A9. Several important factors that had an effect on A9＇s bioflocculant-producing and flocculating activity were studied. A total of 4 g/L Kaolin suspension was used to measure the flocculating activity of the bioflocculant from A9. It was found that maltose and urea were Ag＇s best carbon and nitrogen sources, respectively, and the flocculating activity of the flocculating agent from A9 was markedly increased by the addition of trivalent cations such as Fe^3＋ and Al^3＋; furthermore, the bioflocculant produced by A9 was most effective when the pH value was 6.0.

关键词： bioflocculant flocculating rate Klebsiella sp. screening

Screening of bioflocculant-Producing Strain by Ion Implantation and Flocculating Characteristics of bioflocculants

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Plasma Science and Technology 2008年第3期10卷 394-397页

作者：李培睿李宗伟李宗义秦广雍霍裕平 Key Laboratory of Ion Beam Bio-Engineering Zhengzhou University Zhengzhou 450052 China College of Life Sciences Henan Normal University Xinxiang 453002 China Key Laboratory of Ion Beam Bio-Engineering Zhengzhou University Zhengzhou 450052 China College of Life Sciences Henan Normal University Xinxiang 453002 China

A bioflocculant-producing mutator strain, NIM-192, was screened out through nitrogen ion implanting into F J-7 strain. The results showed that NIM-192 had good genetic stability and high flocculating activity, and the flocculating rate increased by 34.26% than that of the original. Sucrose, complex nitrogen source contained yeast extract, urea and pH 7.0～ 9.0 were chosen as the best carbon source, nitrogen source and initial solution pH for bioflocculant production, respectively. The bioflocculant kept high and stable flocculating activity at alkalinous reaction mixture with a pH beyond 7.0, while the flocculating activity was remarkably reduced when the reaction pH was lower than 7.0. Addition of many cations could obviously increase the flocculating rate, among which Ca^2＋ demonstrated the best effect. The bioflocculant had very strong acid-base stability and thermo-stability.The flocculating rate kept over 86% when pH of the bioflocculant was in a range of 3.0 ～ 12.0, and the change of flocculating activity was not great when heated at 100℃ for 60 rain.

关键词： ion implantation bioflocculant mutation breeding flocculating characteristics

Two-stage pH Control Mode in Batch Fermentation of a Novel bioflocculant from Corynebacterium Glutamicum

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Chemical Research in Chinese Universities 2004年第2期20卷 152-155页

作者： HENing WUXiao-jie DENGXu LUYing-hua LIQing-biao DepartmentofChemicalandBiochemicalEngineering CollegeofChemistryandChemicalEngineeringXiamenUniversityXiamen361005P.R.China

The effect of pH of the fermentation medium on cell growth and the production of a novel bioflocculant(named REA-11) by Corynebacterium glutamicum CCTCC M201005 were investigated. The maximum biomass(2.23 g/L) and flocculating activity(142.2 U/mL) were simultaneously obtained at the 14th hour when the pH value of the culture medium was maintained at 7.0 during the whole fermentation process. The production of REA-11 kept on a trend of increase till the later phase of fermentation process, which resulted in the ultimate flocculating activity of the culture broth to enhance to nearly 100 U/mL at pH 6.0. A two-stage pH control mode was adopted in REA-11 production in which the pH value of the culture medium was controlled at 7.0 during the first 14 h, then decreased to 6.0 that was maintained until the end of the fermentation process. With the two-stage pH control mode, the maximum flocculating activity reached 178.8 U/mL which was 30% higher than that obtained under the condition of pH 7.0 and the biomass enhanced about 15%. Compared with the fermentation process without pH control, REA-11 production and cell growth via the two-stage pH control mode increased 80% and 25%, respectively.

关键词： Corynebacterium glutamicum pH control bioflocculant

Screening of flocculant-producing strains by NTG mutagenesis

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Journal of Environmental Sciences 2005年第3期17卷 494-498页

作者： HUANGXiao-wu CHENGWen HUYong-you SchoolofEnvironmentalScienceandEngineering SouthChinaUniversityofTechmologyGuangzhou510640China SchoolofEnvironmentalScienceandEngineering SouthChinaUniversityofTechmologyGuangzhou510640China

Screening of new microorganism being able to produce efficiently flocculants was carried out. A new model for screening efficient flocculant-producing strains was designed and tested. The results showed that this model for screening efficient flocculant-producing strains is very reliable and can greatly shorten the screening period. 13 flocculant-producing strains were isolated from activated sludge by conventional method. A strain, designated as HHE6, produced the bioflocculant with the turbidity removal 98% for kaolin suspension. Six of 13 strains selected as the original strains were treated with NTG as mutagen, and five mutant strains(HHE-P7, HHE-A8, HHE-P21, HHE-P24, HHE-A26) with high flocculation efficiency was obtained by selection, which exhibited the flocculation rate for kaolin suspension above 90%. Strains HHE6, HHE-P7, and HHE-P24 were classified as Penicillium purpurogenum, HHE-P21 as Penicillium cyclopium, HHE-A26 as Aspergillus versicolor and HHE-A8 as Aspergillus fumigatus, and it is hitherto unreported for biofloccutant-producing strains of Penicillium. The growth of the six strains(HHE6, HHE-P7, HHE-A8, HHE-P21, HHE-P24, HHE-A26) had similar curves, i.e. firstly increasing rapidly, keeping relatively constant then and finally decreasing gradually with cultivation time. The production of bioflocculants by strains showed the similar pattern to strain growth.

关键词： bioflocculant mutation breeding bioflocculant-producing strains Penicillium Aspergillus

STUDIES ON THE SYNTHESIS PATHWAY OF A NOVEL bioflocculant REA-11

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STUDIES ON THE SYNTHESIS PATHWAY OF A NOVEL BIOFLOCCULANT RE...

On an ecology-friendly treatment reagent for oily wastewater

第二届中日化工学术研讨会

作者：关惠琴李寅何宁堵国成陈坚 Key Laboratory of Industrial Biotechnology Ministry of Education Southern Yangtze University Huihe Road 170 Wuxi 214036 Jiangsu China Key Laboratory of Industrial Biotechnology Ministry of Education Southern Yangtze University Huihe Road 170 Wuxi 214036 Jiangsu China Laboratory of Environmental Biotechnology School of Biotechnology Southern Yangtze University Huihe Road 170 Wuxi 214036 Jiangsu China Key Laboratory of Industrial Biotechnology Ministry of Education Southern Yangtze University Huihe Road 170 Wuxi 214036 Jiangsu China Laboratory of Environmental Biotechnology School of Biotechnology Southern Yangtze University Huihe Road 170 Wuxi 214036 Jiangsu China Key Laboratory of Industrial Biotechnology Ministry of Education Southern Yangtze University Huihe Road 170 Wuxi 214036 Jiangsu China Laboratory of Environmental Biotechnology School of Biotechnology Southern Yangtze University Huihe Road 170 Wuxi 214036 Jiangsu China

＜正＞ Corynebacterium glutamicum CCTCC M201005 can produce a bioflocculant REA-11 consisting of galacturonic acid as the main structure unit. Based on literature data, a biosynthesis pathway of REA-11 in C. glutamicum CCTCC M201005 was supposed. The rationality of biosynthesis pathway was demonstrated through （1） correlating the activities of several key enzymes involved in the pathway with the amount of end product polygalacturonic acid;and （2） detecting the existence of several key intermediates in the pathway by HPLC, respectively. It was shown that the activities of UDP-glucose pyrophosphorylase （R2=0. 97）, UDP-galactose epimerase （R2=0. 75） and UDP-galactose dehydrogenase （R2=0. 89） were well correlated with the yield of bioflocculant when different sugars were used as a sole carbon source. Furthermore, key intermediates in the supposed biosynthetic pathway, such as UDP-glucose, UDP-galactose and UDP-glucuronic acid, were detected in cell-free extract.

关键词： Corynebacterium glutamicum bioflocculant galacturonic acid protein-polysaccharide biosynthesis the metabolic pathway

来源： cnki会议评论

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Journal of Chongqing University 2010年第3期9卷 133-138页

作者：刘其友 College of Chemistry and Chemical Engineering China University of Petroleum(Huadong)

bioflocculants have received increasing attention in research because they are low cost,non-toxic,biodegradable,operationally simple and useful in many water and wastewater treatment applications.In this study,we focused mainly on the screening of the flocculant-producing strain and its application in oily wastewater treatment.Bacteria strain B-6-1 was isolated by the pyridine screening method.UPC-1 reagent produced by B-6-1 had efficient flocculating activity.It was deduced that the main component of UPC-1 reagent was polysaccharide through purification and analysis.The influence of dosage of reagent,pH value,temperature and oil concentration on the flocculation effect of UPC-1 reagent was investigated.

关键词： bioflocculant 油性的废水屏蔽生态学友好