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Application of waste frying oils in the biosynthesis of biodemulsifier by a demulsifying strain Alcaligenes sp.S-XJ-1

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Journal of Environmental Sciences 2011年第6期23卷 1020-1026页

作者： Jia Liu Kaiming Peng Xiangfeng Huang Lijun Lu Hang Cheng Dianhai Yang Qi Zhou Huiping Deng Tongji University Shanghai 200092 China College of Environmental Science & Engineering State Key Laboratory of Pollution Control and Resource Reuse Tongji University Shanghai 200092 China

Exploration of biodemulsifiers has become a new research *** waste frying oils（WFOs） as carbon source to synthesize biodemulsifiers has a potential prospect to decrease production cost and to improve the application of biodemulsifiers in the *** this study,a demulsifying strain,Alcaligenes sp.S-XJ-1,was investigated to synthesize a biodemulsifier using waste frying oils as carbon *** was found that the increase of initial pH of culture medium could increase the biodemulsifier yield but decrease the demulsification ratio compared to that using paraffin as carbon *** addition,a biodemulsifier produced by waste frying oils and paraffin as mixed carbon source had a lower demulsification capability compared with that produced by paraffin or waste frying oil as sole carbon ***-batch fermentation of biodemulsifier using waste frying oils as supplementary carbon source was found to be a suitable *** of waste frying oils utilization was studied by using tripalmitin,olein and tristearin as sole carbon sources to synthesize *** results showed saturated long-chain fatty acid was diffcult for S-XJ-1 to utilize but could effectively enhance the demulsification ability of the produced ***,FT-IR result showed that the demulsification capability of biodemulsifiers was associated with the content of C=O group and nitrogen element.

关键词： waste frying oils biodemulsifier fatty acid glyceride Alcaligenes sp.

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Investigation of the relationship between cell surface hydrophobicity and demulsifying capability of biodemulsifier-producing bacteria

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Frontiers in Biology 2018年第5期13卷 358-365页

作者： Hoda Sabati Hossein Motamedi Department of Biology Faculty of Science Shahid Chamran University ofAhvaz Ahvaz Iran Biotechnology and Biological Science Research Center Shahid Chamran University of Ahvaz Ahvaz Iran

BACKGROUND： Cell surface hydrophobicity （CSH） is one of the key physicochemical features of biodemulsifier-producing bacteria that influence their demulsification capability maintenance in petroleum contaminated environments. METHODS： In present study, biodemulsifier-producing bacteria were isolated from petroleum contaminated environments using different isolation media and the correlation between their CSH and demulsifying ability was investigated. The demutsifying ability of isolates was measured through demulsification tests on water in kerosene emulsions. The microbial adhesion to the hydrocarbon （MATH） assay was used to denote their CSH. RESULTS： The evaluation of CSH showed that majority of biodemulsifier producing bacteria have high CSH which indicating a positive correlation between CSH and demulsifying capability. CONCLUSIONS： According to these results it can be concluded that CSH can be used as an indicator for assessment of biodemulsifier-producing bacteria and screening of new isolates for their biodemulsifier production.

关键词： bacterial adhesion biodemulsifier biodemulsifier-producing bacteria demulsification hydrophobicity MATH assay

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