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Improvement of the assimilable organic carbon （AOC） analytical method for reclaimed water

Frontiers of Environmental Science & Engineering 2013年第4期7卷 483-491页

作者： Xin ZHAO Hongying HU Shuming LIU Feng JIANG Xiaolei SHI Mingtang LI Xueqiao XU Environmental Simulation and Pollution Control State Key Joint Laboratory School of Environment Tsinghua University Beijing 100084 China State Environmental Protection Key Laboratory of Microorganism Application and Ris~k Control (MARC)~ Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China College of Resources and Environment Jilin Agriculture University Changchun 130118 China

Microbial growth is an issue of concern that may cause hygienic and aesthetic problems during the transportation and usage of reclaimed water. Assimilable organic carbon （AOC） is an important parameter which determines the heterotrophic bacterial growth potential of water. Pseudomonas fluorescens P17 and Spirillum sp. NOX are widely used to measure AOC in drinking water. The AOC values of various reclaimed water samples determined by P 17 and NOX were compared with those determined by the new strains isolated from reclaimed water in this study. It showed that the conventional test strains were not suitable for AOC measurement of reclaimed water in certain cases. In addition to P17 and NOX, Stenotrophomonas sp. Z J2, Pseudomonas saponi- phila G3 and Enterobacter sp. G6, were selected as test strains for AOC measurement of reclaimed water. Key aspects of the bioassay including inoculum cell density, incubation temperature, incubation time and the pH of samples were evaluated for the newly selected test strains. Higher inoculum density （104 ***-1） and higher incubation temperature （25℃） could reduce the time required for the tests. The AOC results of various collected samples showed the advantages of the method proposed based on those five strains in evaluating the biologic stability of reclaimed water.

关键词： assimilable organic carbon (AOC) bioassay,biological stability reclaimed water test bacterial strains

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