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The adsorption of 2,5-dimer-capto-1,3,4-thiadiazole (DMTD) on copper surface and its binding.behavior

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Chinese Science Bulletin 2001年第5期46卷 387-390页

作者： HUANG Ling SHEN Jian REN Jun MENG Qingjin YU Tsing Research Center of Surface and Interface Chemistry and Engineering Technology Nanjing UniversityNanjing 210093China State Key Laboratory of Coordination Chemistry Coordination Chemistry InstituteNanjing UniversityNanjing 210093China Department of Chemistry Nanjing UniversityNanjing 210093China

The interaction mechanism of 2,5-dimercapto-1,3,4-thiadiazole (DMTD) on copper surface was studied by surface-enhanced Raman scattering (SERS), infrared (IR) and X-ray photoelectron spectroscopy (XPS) techniques. The SERS spectra were obtained. We found that DMTD bound with the copper via its two-excocyclic mercapto groups. The monosulphide salt was formed. At the same time, DMTD coordinated with the Cu+ ion through the excocyclic mercapto groups and polymerized via the Cu+ ion into a one-dimensional polymer chain on the copper surface.

关键词： 2 5-dimercapto-1 3 4-thiadiazole (DMTD) surface-enhanced Raman scattering (SERS) adsorption and binding.

binding.of Uncoupling Protein With Its Ligand

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Chinese Science Bulletin 1994年第19期39卷 1663-1666页

作者：蔡澎林其谁 State Key Laboratory of Molecular Biology Shanghai Institute of Biochemistry Academia Sinica Shanghai 200031 PRC

Uncoupling protein (UCP) is a proton translocator, which is located exclusively in the mitochondrial inner membrane of brown adipose tissue (BAT).It facilitates proton translocation across the membrane,resulting in th... 详细信息

关键词： uncoupling protein ligand dimer GTP binding.

Effect of transmembrane Ca^(2+) gradient on ligand binding.of reconstituted β-adrenergic receptors

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Chinese Science Bulletin 1996年第14期41卷 1214-1218页

作者：杨小毅范高峰黄有国杨福愉 State Key Lab. of Biomacromolecules Institute of Biophysics Chinese Academy of Sdences Beijing 100101 China

It is well known that, under physiological conditions, the cytosolic free Ca2+ in most cells is around 10-7—10-6 mol/L, whereas the extracellular Ca2+ concentration is about 10-3 mol/L. This results in a 1 000—10 000-fold transmembrane Ca2+ gradient. The maintenance of such a concentration gradient has been proved to be of vital importance in cell function. Although a transmembrance Ca2+ gradient exists across the plasma membrance, little attention has

关键词： transmembrane Ca2+ gradient β-adrenergic receptor (β-AR) ligand binding.

FKBP23 in ER binds to BiP specifically

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Science China Chemistry 2004年第1期47卷 17-23页

作者： ZHANG Wanqi, LI Hui, ZHANG Xiaobin, SHEN Ye, WANG Yinong, Ml Huaifeng & HE BinglinState Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Chemical School of Nankai University, Tianjin 300071, China Hygienic School of Tianjin Medical University, Tianjin 300071, China Medical School of Nankai University, Tianjin 300071, China State Key Laboratory of Functional Polymer Materials for Adsorption and Separation Chemical School of Nankai University Tianjin China Hygienic School of Tianjin Medical University Tianjin China Medical School of Nankai University Tianjin China

FKBP23 was found in mouse endoplasmic reticulum (ER) in 1998. It consists of an N-terminal peptidyl-prolyl cis-trans isomerase (PPIase) domain and a C-terminal domain with Ca2+ binding.sites. The fusion protein of mouse FKBP23 and glutathione S-transferase (GST), GST-FKBP23, and the fusion protein of BiP, a member of heat shock protein 70 (Hsp 70) in ER, and GST, GST-BiP, were subcloned in E. coli, expressed and purified. The fusion proteins were restrictively digested by Factor Xa (FaXa) to obtain the free cloned proteins FKBP23 and BiP. With the assay of adsorption of free FKBP23 or BiP with GST-BiP or GST-FKBP23 attached to the Glutathione-Sepharose 4B, the adsorbed FKBP23 or BiP could be detected by Immunoblot. It means that FKBP23 binds to BiP. Furthermore, BiP in leukocyte ER-extract can be adsorbed with GST-FKBP23 attached to the glutathione-Sepharose 4B. It shows that FKBP23 binds to natural BiP in ER, too. These experiments show that a PPIase binds to a molecular chaperone of the Hsp70 family.

关键词： peptidyl-prolyl cis/trans isomerase, FKBP23, BiP, endoplasmic reticulum, specific binding.

Kinetic studies on oxygen binding.to dicobalt(Ⅱ) diporphyrin

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Chinese Science Bulletin 1996年第6期41卷 475-479页

作者：周晓海张绍辉祝德洪黄素秋屈松生 Department of Chemistry Wuhan University Wuhan 430072 China

It has been an important project in the field of biochemistry to search for a seriesof synthetic compounds which have good abilities of carrying oxygen at room temperatureand simulating the hemoglobin’s function of cooperative binding.to oxygen. Uemori et al.and Tabushi et al. have reported that some dicobat(Ⅱ) diporphyrins have thecooperative effect of binding.oxygn in organic solution below 0℃, the Hill coefficients areabout 1.0--1.5. We have reported the thermodynamics of a dicobalt(Ⅱ)

关键词： dicobalt(Ⅱ) diporphyrins model compounds of hemoglobin kinetics of reversible oxygen binding.

Prediction of affinities of peptides for calmodulin

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Science China Chemistry 1996年第2期39卷 113-122页

作者：阎虎生倪爱国刘利平程晓辉何炳林 Institute of Polymer Chemistry Nankai University Tianjin 300071 China

A simple method has been developed to predict the calmodulin-binding.domains of peptides and the affinities of the peptides for calmodulin based on a model for the calmodulin-binding.domains of peptides proposed in a previous paper and the literature data of calmodulin-binding.peptides. The effects of hydrophobitities, α-helix-forming tendencies, and basicities of peptides on their affinities for calmodulin were studied. Model peptides were designed to examine the calmodulin-binding.domain model and the prediction method. The determined dissociation constants of the complexes formed from the model peptides and calmodulin are in good agreement with the predicted results.

关键词： calmodulin peptide binding.