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Mechanisms and biotechnological applications of transcription factors

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Synthetic and Systems Biotechnology 2023年第4期8卷 565-577页

作者： Hehe He Mingfei Yang Siyu Li Gaoyang Zhang Zhongyang Ding Liang Zhang Guiyang Shi Youran Li Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxiJiangsu Province 214122PR China National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing Jiangnan University1800 Lihu AvenueWuxiJiangsu Province 214122PR China Jiangsu Provisional Research Center for Bioactive Product Processing Technology Jiangnan University1800 Lihu AvenueWuxiJiangsu Province 214122PR China

Transcription factors play an indispensable role in maintaining cellular viability and finely regulating complex internal metabolic *** crucial bioactive functions rely on their ability to respond to effectors and concurrently interact with binding *** advancements have brought innovative insights into the understanding of transcription *** this review,we comprehensively summarize the mechanisms by which transcription factors carry out their functions,along with calculation and experimental-based methods employed in their ***,we highlight recent achievements in the application of transcription factors in various biotechnological fields,including cell engineering,human health,and ***,the current limitations of research and provide prospects for future investigations are *** review will provide enlightening theoretical guidance for transcription factors engineering.

关键词： Transcription factors binding sites Database-based prediction Experimental identification Cell engineering Biomanufacturing

Crystal engineering of porous coordination networks for C3 hydrocarbon separation

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SmartMat 2021年第1期2卷 38-55页

作者： Mei‐Yan Gao Bai‐Qiao Song Debobroto Sensharma Michael J.Zaworotko Department of Chemical Sciences Bernal InstituteUniversity of LimerickLimerickRepublic of Ireland

C3 hydrocarbons(HCs),especially propylene and propane,are high‐volume products of the chemical industry as they are utilized for the production of fuels,polymers,and chemical *** for C3 HCs as chemical building blocks is increasing but obtaining them in sufficient purity(>99.95%)for polymer and chemical processes requires economically and energetically costly methods such as cryogenic *** separations using porous coordination networks(PCNs)could offer an energy‐efficient alternative to current technolo-gies for C3 HC purification because of the lower energy footprint of sorbent separations for recycling versus alternatives such as distillation,solvent extraction,and chemical *** this review,we address how the structural modularity of porous PCNs makes them amenable to crystal engineering that in turn enables control over pore size,shape,and *** detail how control over pore structure has enabled PCN sorbents to offer benchmark performance for C3 separations thanks to several distinct mechanisms,each of which is *** also discuss the major challenges and opportunities that remain to be addressed before the commercial development of PCNs as advanced sorbents for C3 separation becomes viable.

关键词： binding sites C3 hydrocarbon PCNs separation

[~3H] TYROSINE binding TO PLASMA MEMBRANE PREPARATION OF RAT CORPORA LUTEA

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Science in China,Ser.B 1988年第12期31卷 1454-1466页

作者：高而威程治平 Department oF Physiology Harbin Medical University

Plasma membrane preparations of rat corpora lutea have been incubated with [3H]tyrosine. [3H]-tyrosine binding sites are demonstrated and Scatchard analysis shows that there exist two types of binding sites, one with high affinity and low capacity, the other with low affinity and high capacity. The kinetics studies demonstrate that the [3H]tyrosine binding to the two types of binding sites is reversible and the speed of binding to the high affinity type is faster than that to the low affinity type. The analysis of the chemical structure of tyrosine analogues and related compounds with respect to the specificity of the binding sites reveal that both types of binding sites show specificity, but the specificity of the high affinity sites is higher than that of the low affinity sites. The relations of tyrosine structure to binding processing and to tyrosine inhibitory action on hCG-induced progesterone production are discussed. It is suggested that the high affinity binding sites might be regarded as 'ty

关键词： tyros ne receptor binding sites corpora lutes progesterone.

SOME FACTORS AFFECTING [3H]TYROSINE binding TO PLASMA MEMBRANE PREPARATION OF RAT CORPORA LUTEA in vitro

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Science China Chemistry 1988年第12期31卷 1467-1473页

作者：高而威程治平 Department oF Physiology Harbin Medical University Department oF Physiology

Temperature, pH, enzymes and ions affected the two types of [H]tyrosine binding sites differently. The optimal temperature and pH for the high affinity sites were 24 C and 7.4, and for the low affinity sites 37℃ and 7.0, respectively. The [H]tyrosine binding to the high and low affinity sites was not observed at 55℃, nor at pH 6.0 or pH 8.5. Trypsin reduced [H]tyrosine binding to the high affinity sites, but had no effect on the binding to the low affinity sites. Neuraminidase reduced [H]tyrosine binding to the low affinity sites, but had no effect on the binding to the high affinity sites. None of cycloheximide, kCG, cAMP, and progesterone affected [H]tyrosine binding.

关键词： tyrosine receptor binding sites corpora lutea progesterone.

Influence of strain on hydrogenic impurity states in a GaN/ Al_xGa_(1-x)N quantum dot

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Optoelectronics Letters 2009年第2期5卷 85-88页

作者：张彬闫祖威 Department of Physics Inner Mongolia UniversityHohhot 010021 China Colleges of Sciences Inner Mongolia Agricultural UniversityHohhot 010018 China

Within the effective-mass approximation, we calculated the influence of strain on the binding energy of a hydrogenic donor impurity by a variational approach in a cylindrical wurtzite GaN/AlxGa1-xN strained quantum dot, including the strong built- in electric field effect due to the spontaneous and piezoelectric polarization. The results show that the binding energy of impurity decreases when the strain is considered. Then the built-in electric field becomes bigger with the Al content increasing and the bin...

关键词： Aluminum binding sites Electric field effects Gallium Nuclear energy Potential energy Semiconductor quantum dots Semiconductor quantum wells Zinc sulfide

Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells

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Chinese Optics Letters 2013年第8期11卷 76-79页

作者：余杰佟训靓李成彬黄一宁叶安培 Key Laboratory for the Physics and Chemistry of Nanodevices School of Electronics Engineering and Computer SciencePeking University Department of Neurology Peking University First Hospital

We carry out in situ single-molecule measurements of the specific interaction between apolipoprotein A-I （apoA-I） and ATP binding cassette transporter A1 （ABCA1） on THP-1 cells. Single-molecule force spectroscopy shows that similar to normal apoA-I, the dysfunctional apoA-I from diabetes patients interacts with ABCA1 via two different binding sites on the cells. The strength of dysfunctional apoA-I binding to a high-capacity binding site is 26.5±4.9 pN. The minor direct apoA-I/ABCA1 binding strength is 56.7±4.1 pN. These results facilitate a pathological understanding of the mechanisms that underlie the specific interaction of aDoA-I and ABCA1 at the single-molecule level.

关键词： binding sites Optical tweezers

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Polymorphisms and functions of the aldose reductase gene 5' regulatory region in Chinese patients with type 2 diabetes mellitus

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Chinese Medical Journal 2002年第2期115卷 209-213页

作者：李清解谢平黄建军谷亚鹏曾卫民宋惠萍中南大学湘雅医学院生物化学教研室，长沙，410078

OBJECTIVE: To screen the 5' regulatory region of the aldose reductase (AR) gene for genetic variabilities causing changes in protein expression and affecting the promoter function. METHODS: The screenings were carried out by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). All SSCP variants were submitted for DNA sequencing and inserted into the plasmid chloromycetin acetyl transferase (CAT) enhancer vector. The constructs were used to transfect Hela cells, and CAT assays were performed to assess promoter activity. Gel mobility shift and footprinting assays were also performed to determine the interaction between the DNA and nuclear proteins. RESULTS: Two polymorphisms, C (-106) T and C (-12) G, were identified in the regulatory region in 123 Chinese control subjects and 145 patients with type 2 diabetes mellitus. The frequencies of genotypes WT/WT, WT/C (-12) G and WT/C (-106) T were not significantly different between the subjects and patients. In the patients with and without retinopathy, frequencies of WT/C (-106) T were 31.5% and 17.5% (P 0.05) respectively. The total frequency of WT/C (-12) G and WT/C (-106) T in patients with retinopathy was 41.8%, significantly higher than that (20.0%) in patients without retinopathy (P

关键词： 5' Flanking Region Adult Aldehyde Reductase binding sites China Chloramphenicol O-Acetyltransferase DNA DNA Footprinting Diabetes Mellitus, Type 2 Electrophoretic Mobility Shift Assay Female Hela Cells Humans Male Middle Aged Mutation Polymerase Chain Reaction Polymorphism, Genetic Polymorphism, Single-Stranded Conformational Recombinant Fusion Proteins Regulatory Sequences, Nucleic Acid Research Support, Non-U.S. Gov't Sequence Analysis, DNA Transcription, Genetic

Interactions Between Anticancer Pt(II) complexes and Human Erythrocyte Spectrin *

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Journal of Chinese Pharmaceutical Sciences 1998年第3期7卷 9-14页

作者：杨晓改李荣昌北京医科大学生物无机及无机药物化学系

The interactions between human erythrocyte spectrin(SP) and Pt(II) complexes with different composition and configuration were studied by fluorescence and circular dichroism spectra. The results showed that there are 4.7×10 2 binding sites of cisplatin(CDDP) in a spectrin tetramer(SPT). Among them, about 70 sites with apparent binding constant K 1>3.47×10 6 were of highest affinity, 1.8×10 2 sites with K 2 = 3.47×10 6 were of high affinity, and other 2.2×10 2 sites with K 3 = 8.77×10 5 were of low affinity. The conformation change of spectrin, depending on the concentration of Pt(II) complex and molar ratio(R) of Pt(II) complex to spectrin, was induced by the binding of Pt(II) complexes. It indicated that the interaction of both CDDP and cis diaquodiamine platinum(DADP) with SP followed a two step first order kinetic process in the first stage (1 h), and the kinetic constants were determined. In the second stage, the induced conformation change, polymerization and depolymerization of SP were probably involved. It was noticed that in the reaction of SP and Pt(II) complexes with 1,2 cyclohexanediammine isomers as chiral carrier ligand, stereo matching played a more important role than the affinity of Pt(II) to thiol groups of SP.

关键词： Pt (II) complex Spectrin Conformation binding sites Kinetic Chiral

Screening of SLE-susceptible SNPs in one Chinese family with systemic lupus erythematosus

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Screening of SLE-susceptible SNPs in one Chinese family with...