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World Journal of Gastroenterology 2009年第23期15卷 2887-2892页

作者： Angèle PM Kerckhoffs Melvin Samsom Michel E van der Rest Joris de Vogel Jan Knol Kaouther Ben-Amor Louis MA Akkermans Gastrointestinal Research Unit Department of Gastroenterology and Surgery University Medical Center Utrecht Heidelberglaan 100 F02.618 3584CX Utrecht The Netherlands BioVisible BV LJ Zielstraweg 1 9713 GX Groningen The Netherlands Danone Research-Centre for Specialized Nutrition Bosrandweg 20 6704PH Wageningen The Netherlands

AIM: To determine the composition of both fecal and duodenal mucosa-associated microbiota in irritable bowel syndrome (IBS) patients and healthy subjects using molecular-based techniques. METHODS: Fecal and duodenal mucosa brush samples were obtained from 41 IBS patients and 26 healthy subjects. Fecal samples were analyzed for the composition of the total microbiota using fluorescent in situ hybridization (FISH) and both fecal and duodenal brush samples were analyzed for the composition of bif idobacteria using real-time polymerase chain reaction. RESULTS: The FISH analysis of fecal samples revealed a 2-fold decrease in the level of bifidobacteria (4.2 ± 1.3 vs 8.3 ± 1.9, P bifidobacteria levels in both fecal and duodenal brush samples of IBS patients compared to healthy subjects indicate a role for microbiotic composition in IBS pathophysiology.

关键词： Irritable bowel syndrome Gut microbiota bifidobacteria Bifidobacterium catenulatum

Anti-inflammatory effects of bifidobacteria by inhibition of LPS-induced NF-κB activation

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World Journal of Gastroenterology 2006年第23期12卷 3729-3735页

作者： Christian U Riedel Francis Foata David Philippe Oskar Adolfsson Bernhard J Eikmanns Stephanie Blum Department of Microbiology and Biotechnology University of Ulm89068 UlmGermany Immunology GroupNutrition and Health Dept.Nestlé Research CenterPO Box 44CH-1026 LausanneSwitzerland Immunology Group Nutrition and Health Dept.Nestlé Research CenterPO Box 44CH-1026 LausanneSwitzerland Department of Microbiology and Biotechnology University of Ulm89068 UlmGermany

AIM： Different strains of bifidobacteria were analysed for their effects on HT-29 intestinal epithelial cells （IECs） in in vitro models both of the non-inflamed and inflamed intestinal epithelium. METHODS： A reporter gene system in HT-29 cells was used to measure levels of NF-KB activation after challenge with bifidobacteria or after bacterial pre-treatment following LPS challenge. IL-8 protein and pro-inflammatory gene expression was investigated using normal HT-29 cells. RESULTS： None of the bifidobacteria tested induced activation of nuclear factor κB （NF-κB） indicating that bifidobacteria themselves do not induce inflammatory events in IECs. However, six out of eight bifidobacteria tested inhibited lipopolysaccharide- （LPS-） induced NF-κB activation in a dose- and strain-dependent manner. In contrast, NF-κB activation in response to challenge with tumor necrosis factor-α （TNF-α） was affected by none of the tested bifidobacteria, indicating that the inhibitory effect of bifidobacteria is specific for LPS-induced inflammation in IECs. As shown with two of the six inhibitionpositive bifidobacteria, LPS-induced inhibition of NF-κB activation was accompanied by a dose-dependent decrease of interleukin 8 （IL-8） secretion and by lower mRNA levels for IL-8, TNF-α, cyclooxygenase 2 （Cox-2）, and intercellular adhesion molecule 1（ICAM-1）. CONCLUSION： Some strains of bifidobacteria are effective in inhibiting LPS-induced inflammation and thus might be appropriate candidates for probiotic intervention in chronic intestinal inflammation.

关键词： NF-κB bifidobacteria Anti-inflammatory LPS

Effect of EPEC endotoxin and bifidobacteria on intestinal barrier function through modulation of toll-like receptor 2 and toll-like receptor 4 expression in intestinal epithelial cell-18

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World Journal of Gastroenterology 2017年第26期23卷 4744-4751页

作者： Xia Yang Xian-Chun Gao Jun Liu Hong-Yu Ren Division of Gastroenterology Union Hospital Tongji Medical College Huazhong University of Science and Technology

AIM To investigate toll-like receptor 2(TLR2) and TLR4 expression, following bifidobacteria and low-dose EPEC endotoxin treatment, and intestinal barrier function in rat intestinal epithelial cell-18(IEC-18).METHODS Six experimental groups were established-normal control, EPEC, bifidobacteria infantis(B. infantis), B. longum, B. bifidum, and B. youth groups. Optimal EPEC endotoxin concentration, bifidobacteria fold dilution, and treatment duration were determined. Quantitative real-time polymerase chain reaction and western blot, respectively, were conducted to detect TLR2 and TLR4 m RNA and protein expression in IEC-18 cells. Transepithelial electrical resistance(TEER) was measured by the EVOM chopstick voltohmmeter in each group. All experiments were conducted in triplicate and data were analyzed on SPSS *** TLR2 and TLR4 m RNA and protein expression in the EPEC group were significantly higher than in the control group(P bifidobacteria protect IEC-18 cells against injury by down-regulating TLR2 and TLR4 expression and enhance intestinal barrier function to protect the intestinal epithelial cells from pathogenic invasion.

关键词： bifidobacteria Intestinal barrier function Intestinal epithelial cells

SIGNAL MECHANISM OF INHIBITION OF bifidobacteria ON GROWTH OF COLON CANCER

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Chinese Journal of Cancer Research 2005年第2期17卷 145-149页

作者：戴建宜王立生朱惠明潘令嘉马晓东张亚历周殿元 Department of Gastroenterology Shenzhen Municipal People’s Hospital Shenzhen 518020 Department of Gastroenterology Nanfang Hospital Guangzhou 510515

Objective: To explore the antitumor mechanisms of bifidobacteria adolescence in vivo. Methods: The content of extracellular signal regulated proteins (ERK)1/2, C-Jun N-terminal kinase (JNK), p38, c-fos and c-jun in nude mouse transplanted large bowel carcinoma was detected by using laser confocal microscopy. The expression of NF-κB was determined by immunohistochemistry. Results: After the nude mouse transplanted tumor was treated with bifidobacteria, the average fluorescent strength of ERK1/2, JNK, c-fos and c-jun was significantly lower than that in tumor control group (P0.05). The positive cell density of NF-κB in large bowel carcinoma transplantation tumors in Bifidobacterium injection group was markedly lower than that in tumor group(Pbifidobacteria adolescence could markedly decrease the activity of ERK1/2 and JNK, the expression c-fos and c-jun, and the activity of NF-κB.

关键词： bifidobacteria Colon cancer Signal transduction C-jun N-terminal kinase Extracellular signal- regulated protein kinase NF-κB

bifidobacteria as Potential Functional Starter Cultures: A Case Study by MSc Students in Food Science and Technology (University of Foggia, Southern Italy)

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Food and Nutrition Sciences 2012年第1期3卷 55-63页

作者： Antonio Bevilacqua Maria Teresa Cagnazzo Cataldo Caldarola Emanuela Ciuffreda Anna Rita Dragano Stefania Franchino Rossana Lauriola Angela Pacifico Maria Rosaria Corbo Milena Sinigaglia 不详

This research paper was the results of activity of MSc students of Food Science and Technology, attending the class “Biotechnology of Functional Starter”. Five strains of bifidobacteria (Bifidobacterium animalis subsp. lactis;B. longum subsp. infantis;B. breve;B. animalis subsp. animalis;B. bifidum) were evaluated in order to assess their suitability as functional starter cultures, by studying the following technological and probiotic traits: growth at different temperatures, NaCl amounts and pH values;acidifying ability;metabolism (arginin deamination, esculin hydrolysis, acetoin production);survival at low pH and in presence of bile salts;hydrophobic properties;antibiotic resistance. After laboratory assays and strain selection through a multivariate analyses, it was highlighted that B. longum subsp. infantis and B. animalis subsp. lactis represent a good compromise as potential functional starter cultures, as B. animalis subp. lactis showed a high growth index at pH 5 and good values at 25?C and 30?C, as well as the minimal viability loss at pH 2.5. B. longum subsp. infantis DSMZ 20088 was the best microorganism for its growth index in presence of 6.5% of salt added and at 25?C and 30?C.

关键词： bifidobacteria Functional Starter Probiotic Properties Technological Characterization

bifidobacteria Upregulate Expression of Toll-Like Receptor Negative Regulators Counteracting Enterotoxigenic <i>Escherichia coli</i>Mediated Inflammation in Bovine Intestinal Epitheliocytes

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Open Journal of Veterinary Medicine 2013年第2期3卷 143-155页

作者： Kozue Murata Julio Villena Yohsuke Tomosada Risa Hara Eriko Chiba Tomoyuki Shimazu Hisashi Aso Yoshihito Suda Noriyuki Iwabuchi Jin-Zhong Xiao Tadao Saito Haruki Kitazawa Food and Feed Immunology Group Laboratory of Animal Products Chemistry Graduate School of Agricultural Science Tohoku University Sendai Japan

We previously established a bovine intestinal epithelial cell line (BIE cells) and showed that BIE cells are useful in vitro model system for the study of interactions between pathogenic and beneficial microorganisms and bovine intestinal epithelial cells (IECs). In the present study, we aimed to select potential immunomodulatory bifidobacteria that may be used to beneficially modulate the inflammatory response in bovine IECs. We also aimed to gain insight into the molecular mechanisms involved in the anti-inflammatory effect of bifidobacteria by evaluating the role of Toll-like receptor (TLR)-2 and TLR negative regulators in the regulation of proinflammatory cytokines production and MAPK, NF-κB and PI3K pathways activation in BIE cells. Five bifidobacteria strains were evaluated in this study and according to their capacity to modulate the inflammatory response of BIE cells. Despite the unique effect of each strain, four common points were found when comparing the effect of the high and moderate anti-inflammatory strains: 1) Upregulation of TLR negative regulators and the intensity of that upregulation was related to the different immunomodulatory capacity of each bifidobacteria strain;2) The balance between MAPK activation and MKP-1 upregulation affected the anti-inflammatory effect of bifidobacteria in BIE cells;3) The inhibition of PI3K pathway was related to the anti-inflammatory effect of bifidobacteria;4) The immunoregulatory effect of bifidobacteria in BIE cells is partially dependent on TLR2. This study shows that BIE cells can be used for the selection of immunoregulatory bifidobacteria and for studying the mechanisms involved in the protective activity of immunobiotics against TLR4-induced inflammatory damage. In addition, we have demonstrated that the anti-inflammatory effect of bifidobacteria was achieved by a complex interaction of multiple TLRs negative regulators as well as the inhibition/activation of multiple signaling pathways.

关键词： Bovine Intestinal Epithelial Cells bifidobacteria ETEC TLRs Negative Regulators

A preliminary clinical study of bifidobacteria preparation on the treatment of diarrhea in severely burned patients

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Chinese Medical Journal 1998年第4期 94-95页

作者：陈军张雅萍肖光夏

Objective To investigate the role of bifidobacteria preparation in the prevention of diarrhea in severely burned patients. Methods Forty three severely burned patients who were inflicted by diarrhea were included in this study. The changes of intestinal microflora were observed. Results The intestinal flora of all patients with diarrhea changed greatly before treatment. The proportions of bifidobacteria and bacteroid decreased significantly, whereas those of aerobe and Candida increased relatively. The ratio of anaerobe to aerobe decreased. As a result, the patients' intestinal flora were distorted. After six days treatment of bifidobacteria, diarrhea in most patients ceased and the intestinal microflora restored. Conclusion bifidobacteria feeding plays an important role in restoring intestinal microflora and stopping diarrhea in severely burned patients.

关键词： study clinical 陈军 bifidobacteria

bifidobacteria and host health——an update

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Science Foundation in China 2018年第1期26卷 1-17页

作者： CHEN Wei School of Food Science and Technology Jiangnan University Wuxi 214122 China

The intestinal microbiota has a vital role in human ***,Bifidobacteriumis a genus that is crucial for health and different *** potentially functional investigation of Bifidobacteriumis needed,which will be of great importance to human *** review aims to describe bifidobacteria compositional changes associated with different ages,to highlight the impact of bifidobacteria on health and disease,and to indicate their clinical *** longum,***,and *** dominant in infants,whereas *** *** are more prevalent in *** improve the treatment of diarrhea,inflammatory bowel disease(IBD),colon irregularity,colorectal cancer,necrotizing enterocolitis,diabetes and disorder of carbohydrate metabolism.

关键词： bifidobacteria Health effects Probioties

Deep Exploration of bifidobacteria through Metabolomics Study

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Journal of Biosciences and Medicines 2018年第5期6卷 57-62页

作者： Juan Li Yatao Jiang Yihao Shen Qingzhi Li Zhongke Sun Institute of Food and Drug Inspection College of Life Sciences and Agronomy Zhoukou Normal University Zhoukou China Library of Zhoukou Normal University Zhoukou China

bifidobacteria are probiotic bacteria with multiple health-promoting properties for human being. The global market for probiotics, especially for bifidobacteria is booming. However, the entire market is still at an early stage as there is nearly no fine products developed yet except the whole bacterial cells. The maturation of metabolomics technologies make it possible to study complex mixture with high-throughput, comprehensive maps and libraries. Therefore, we prospect that metabolomics studies mainly based on liquid/gas chromatography-mass spectrometry (LC/GC-MS) can deepen our understanding in detail during the study of metabolic mechanisms of bifidobacteria. These studies can be conducted at three phases, including non-targeted, targeted metabolomic analysis of bifidobacteria, and specific metabolites production through metabolic engineering and fermentation. Metabolomic studies of bifidobacteria will allow us to fully explore their metabolic mechanisms and to utilize metabolites that contribute to human health. In particular, bifidobacteria derived conjugated linoleic acids and bacteriocins are two kinds of fined products that may have great potentials in the future and can be used as food additives.

关键词： bifidobacteria Metabolomics Metabolites Conjugated Linoleic Acid Probiotics Derived Fine Products

Bioinformatic Survey of S-Layer Proteins in bifidobacteria

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Computational Molecular Bioscience 2018年第2期8卷 68-79页

作者： Juan Li Yihao Shen Yatao Jiang Le He Zhongke Sun Institute of Food and Drug Inspection College of Life Sciences and Agronomy Zhoukou Normal University Zhoukou China Library of Zhoukou Normal University Zhoukou China

Surface layer (S-layer) proteins are one of the most commonly observed cell envelope components in both Archaea and Bacteria. It has versatile functions and holds considerable application potential in biotechnology. bifidobacteria are representative probiotics conferring health promoting properties. However, there is little study of S-layer in bifidobacteria yet. The distribution and characteristics of S-layer in bifidobacteria are unknown. In this study, search for S-layer protein in the identical protein groups in NCBI yielded 49 hits belonging to bifidobacteria. These proteins were annotated as either “S-layer (domain) protein” or “putative S-layer (y) domain protein” that distributed among 26 species of Bifidobacterium genus. Multiple alignments suggest S-layer proteins are relatively conservative. Phylogenetic analysis of 24 S-layer (domain) protein sequences groups them into three distinct clusters, with the majority species in Cluster-2. S-layer (domain) protein has a universe motif DUF4381, though its function is unknown. Meanwhile, two other motifs CARDB and EphA2_TM involved in cell adhesion and cell signaling respectively, presented in most S-layer (domain) protein in bifidobacteria. All S-layer proteins have a typical N-terminal Sec-dependent signal peptide and a C-terminal trans-membrane region. Homological modeling of representative S-layer proteins from each cluster revealed a few unique structural features. All representative S-layer proteins have a plenty of β-meander motif that exclusively composed by β-barrel structural architectures linked together by hairpin loops.

关键词： bifidobacteria S-layer Domain Protein Phylogeny Homology Modeling Domain