检索结果-南通市图书馆

benfotiamine prevents increased β-amyloid production in HEK cells induced by high glucose

Neuroscience Bulletin 2012年第5期28卷 561-566页

作者： Xiao-Jing Sun Lei Zhao Na Zhao Xiao-Li Pan Guo-Qiang Fei Li-Rong Jin Chun-Jiu Zhong Department of Neurology Zhongshan Hospital & Shanghai Medical College Fudan University Shanghai 200032 China State Key Laboratory of Medical Neurobiology Institute of Brain Science Fudan University Shanghai 200032 China

Objective To determine whether high glucose enhances β-amyloid （Aβ） production in HEK293 Swedish mutant （APPsw） cells with Aβ precursor protein （APP） overexpression, and whether under this condition benfotiamine reduces the increased Aβ production. Methods HEK293 APPsw cells were cultured with different concentrations of glucose for different times. TheAβ content in the supernatant was determined by ELISA. To investigate the mechanism by which benfotiamine reduced Aβ production, glycogen synthase kinase-3 （GSK-3） activity and expression were measured after the cells were cultured with 5.5 g/L glucose for 12 h. Results With 1.0, 3.0, 4.5, 5.5, 6.5, 7.5, 8.5, or 10.5 g/L glucose, Aβ production by HEK293 APPsw cells was highest in the presence of 5.5 g/L glucose for 6 and 12 h. The difference in Aβ content between 5.5 and 1.0 g/L was most marked after incubation for 12 h. benfotiamine at 20 and 40 μg/mL significantly reduced Aβ production in cells incubated with 5.5 g/L glucose for 12 h. Moreover, 40 μg/mL benfotiamine significantly enhanced the ratio of phosphorylated GSK-3 to total GSK-3, together with consistent down-regulation of GSK-3 activity. Conclusion High glucose increases Aβ production by HEK293 APPsw cells while benfotiamine prevents this increase. This is correlated with the modulation of GSK-3 activity.

关键词： Alzheimer’s disease β-amyloid high glucose benfotiamine glycogen synthase kinase

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

benfotiamine supplementation prevents oxidative stress in anterior tibialis muscle and heart

引用

Journal of Integrative Medicine 2019年第6期17卷 423-429页

作者： Alisson de Carvalho Goncalves Einy Jéssika Siqueira Moreira Guilherme Vannucchi Portari Laboratory of Experimental Nutrition Health Sciences InstituteFederal University of Triangulo MineiroUberabaMG 38025-440Brazil Department of Nutrition Health Sciences InstituteFederal University of Triangulo MineiroUberabaMG 38025-440Brazil

Objective: This study aimed to evaluate the influence of oral supplementation with benfotiamine on oxidative stress in the liver, heart and muscles of endurance-trained ***: Twenty-five male BALB/c mice were allocated to the following treatment groups: standard diet and sedentary activity(Sta–Sed), benfotiamine–supplemented diet and sedentary activity(Ben–Sed),standard diet and training activity(Sta–Tr) and benfotiamine–supplemented diet and training activity(Ben–Tr). The training comprised 6 weeks of endurance swimming training. The concentration of thiobarbituric acid reactive substances(TBARS), carbonylated proteins, total thiols and non-protein thiols was analyzed in the liver, heart and tibialis anterior ***: In the muscle, TBARS concentration in the Sta–Sed group was higher than that in other groups;in the heart, TBARS concentration in the Sta–Sed and Ben–Tr groups was higher than that in the Ben–Sed group. The carbonyl content of the muscle tissues was higher in the Sta–Sed group than in both supplemented groups. In liver, the carbonyl content was lower in the Ben–Sed group than in the Sta–Sed *** level of total thiols was lower in the Ben–Sed group than in the Sta–Tr group. In the heart, the level of total thiols was higher in the Ben–Sed group than in the Ben–Tr group. The concentration of non-protein thiols in the muscle was higher in the Ben–Sed group than in the Ben–Tr group, whereas in the heart, concentration of non-protein thiols of Sta–Tr group was lower than that of Sta–Sed ***: The results show that benfotiamine is an efficient antioxidant for the anterior tibialis muscle and heart;however, swimming training did not alter redox status.

关键词： benfotiamine Thiamine Endurance exercise Oxidative stress Vitamins

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Post-Marketing Surveillance of Fixed Dose Combination of Methylcobalamin, Alpha Lipoic Acid, Folic Acid, Biotin, benfotiamine &Vitamin B6-Nutripathy for the Management of Peripheral Neuropathy

引用

Journal of Diabetes Mellitus 2014年第2期4卷 124-132页

作者： Manish Maladkar Chitra Tekchandani Urja Dave Aristo Pharmaceuticals Pvt. Ltd. Mumbai India

Background: Peripheral neuropathy is a commonly encountered troublesome condition which is often disabling & worsens when left untreated. Traditional neuropathic pain medications primarily provide symptomatic relief;however, the pathogenesis of nerve damage remains unresolved. Extensive literature survey reveals that patients with peripheral neuropathy experience significant benefits with the use of B-vitamins like methylcobalamin (B12), folic acid (B9), biotin (B7), benfotiamine (B1) and pyridoxine (B6). The other well documented antineuropathic agents include alpha lipoic acid, glutathione, omega fatty acids, myoinositol, certain trace elements, etc. Materials and Methods: A multicentre, prospective, open-label, non-comparative clinical study was carried out in 497 patients with peripheral neuropathy. A fixed dose combination of methylcobalamin, alpha lipoic acid (ALA), folic acid, biotin, benfotiamine & vitamin B6 capsule was orally administered once daily for 12 weeks. Results: Treatment led to significant reduction from baseline score in various neuropathy symptoms from the 4th week itself. After 12 weeks of treatment, the mean pain score declined by 78.0%, numbness by 92.1% and muscle weakness by 96.9%. Also, there was 96.0% & 99.2% reduction in tingling & burning sensation respectively. No serious adverse events were ***: The current study confirms that fixed dose combination of methylcobalamin, ALA, folic acid, biotin, benfotiamine & vitamin B6 is effective & well tolerated in the management of peripheral neuropathy.

关键词： Peripheral Neuropathy Methylcobalamin Alpha Lipoic Acid Folic Acid Biotin benfotiamine Vitamin B6

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroprotective Effects of Hesperidin and benfotiamine against Paraquat Induced Spinal Cord Neurotoxicity in Adult Male Albino Rats

引用

Occupational Diseases and Environmental Medicine 2021年第3期9卷 139-164页

作者： Dalia M. Amin Shimaa H. Ameen Marwa T. Abaza Forensic Medicine and Clinical Toxicology Department Faculty of Medicine Zagazig University Zagazig Egypt

Introduction: There are extensive people exposures to paraquat (PQ) herbicide resulting in human health hazards. Aim of the Work: To compare the beneficial neuroprotective effects of hesperidin and benfotiamine on paraquat (PQ)-induced spinal cord neurotoxic effects in rats. Materials and Methods: Rats were divided into 4 groups as following: control, paraquat (PQ 20.8 mg/kg, oral gavage (e.g.)), paraquat + benfotiamine (50 mg/kg, oral gavage (e.g.)) and paraquat + hesperidin (40 mg/kg, oral gavage (e.g.)). PQ is given as the previous dose. Rats are treated 6 days per week. Results: There was a significant increased mean value of malondialdehyde associated with a significant reduction in the content of reduced glutathione and antioxidant enzymes activities associated with a significant increase in Serum phosphorylated neurofilament-H, neurospecific enolase and s100 levels were recorded and significant spinal cord histopathological changes in paraquat treated group as compared to their corresponding values in the control group and immunohistochemical examination confirmed these results. Upon supplementation with benfotiamine and hesperidin to paraquat treated rats, there was a significant decrease in the mean values of malondialdehyde associated with a marked increase in the content of reduced glutathione and antioxidant enzymes activities associated with a significant decrease in Serum phosphorylated neurofilament-H, neurospecific enolase and s100 levels were also recorded with significant improvement of spinal cord architecture when compared with the paraquat treated group. Conclusion: The use of benfotiamine and hesperidin produced a significant protection against all of the above-mentioned changes.

关键词： benfotiamine Hesperidin Paraquat Spinal Cord Toxicity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evidence for altered thiamine metabolism in diabetes: Is there a potential to oppose gluco- and lipotoxicity by rational supplementation?

引用

World Journal of Diabetes 2014年第3期5卷 288-295页

作者： Luká? Pácal Katarína Kuricová Katerina Kaňková Department of Pathophysiology Faculty of MedicineMasaryk University

Growing prevalence of diabetes(type 2 as well as type 1) and its related morbidity due to vascular complications creates a large burden on medical care worldwide. Understanding the molecular pathogenesis of chronic micro-, macro- and avascular complications mediated by hyperglycemia is of crucial importance since novel therapeutic targets can be identified and tested. Thiamine(vitamin B1) is an essential cofactor of several enzymes involved in carbohydrate metabolism and published data suggest that thiamine metabolism in diabetes is deficient. This review aims to point out the physiological role of thiamine in metabolism of glucose and amino acids, to present overview of thiamine metabolism and to describe the consequences of thiamine deficiency(either clinically manifest or latent). Furthermore, we want to explain why thiamine demands are increased in diabetes and to summarise data indicating thiamine mishandling in diabetics(by review of the studies mapping the prevalence and the degree ofthiamine deficiency in diabetics). Finally, we would like to summarise the evidence for the beneficial effect of thiamine supplementation in progression of hyperglycemia-related pathology and, therefore, to justify its importance in determining the harmful impact of hyperglycemia in diabetes. Based on the data presented it could be concluded that although experimental studies mostly resulted in beneficial effects, clinical studies of appropriate size and duration focusing on the effect of thiamine supplementation/therapy on hard endpoints are missing at present. Moreover, it is not currently clear which mechanisms contribute to the deficient action of thiamine in diabetes most. Experimental studies on the molecular mechanisms of thiamine deficiency in diabetes are critically needed before clear answer to diabetes community could be given.

关键词： Diabetes Thiamine Vitamin B1 Transketolase benfotiamine Hyperglycemia Nephropathy Metabolic syndrome Cardiovascular disease Chronic kidney disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：