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Species authentication of commercial beef jerky based on PCR-RFLP analysis of the mitochondrial 12S rRNA gene

Journal of Genetics and Genomics 2010年第11期37卷 763-769页

作者： Shi-Yi Chen Yi-Ping Liu Yong-Gang Yao Key Laboratory of Animal Models and Human D&ease Mechanisms of the Chinese Academy of Sciences & Yunnan Province Kunming Institute of Zoology Kunming 650223 China Research Institute of Animal Genetics and Breeding Siehuan Agricultural University Ya'an 625014 China

In this study, we determined species-specific variations by analyzing the mitochondrial 12S rRNA gene sequence variation （-440 bp） in 17 newly obtained sequences and 90 published cattle, yak, buffalo, goat, and pig sequences, which represent 62 breeds and 17 geo- graphic regions. Based on the defined species-specific variations, two endonucleases, Alu I and Bfa I, were selected for species authentication using raw meat/tissue samples and the PCR-RFLP method. Goat and pig were identified using the Alu I enzyme, while cattle, yak, and buffalo were identified by digestion with Bfa I. Our approach had relatively high detection sensitivity of cattle DNA in mixed cattle and yak products, with the lowest detectable threshold equaling 20% of cattle DNA in a mixed cattle/yak sample. This method was successfully used to type commercial beef jerky products, which were produced by different companies utilizing various processing technologies. Our results show that several yak jerky products might be implicated in commercial fraud by using cattle meat instead of yak meat.

关键词： 12S rRNA gene PCR-RFLP meat species identification beefjerky commercial fraud

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