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Six novel steroids from culture of basidiomycete Polyporus ellisii

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Natural Products and Bioprospecting 2012年第6期2卷 240-244页

作者： Shuang WANG Ling ZHANG Liang-Yan LIU Ze-Jun DONG Zheng-Hui LI Ji-Kai LIU State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China University of Chinese Academy of Sciences Beijing 100049China

Investigation of the culture of basidiomycete Polyporus ellisii led to the isolation of a novel compound 3β,9α,15α-trihydroxy-(22E,24R)-10(5→4)-abeo-ergosta-6,8(14),22-trien-5-one(1)with a new 5/7/6/5 ring system of ergosterol *** addition,five new steroids,5β,6β-epoxy-3β,7α,9α-trihydroxy-(22E,24R)-ergosta-8(14),22-dien-15-one(2),5β,6β-epoxy-3β,7α-dihydroxy-(22E,24R)-ergosta-8(14),22-dien-15-one(3),5α,6α-epoxy-3β,9α,15α-trihydroxy-(22E,24R)-ergosta-8(14),22-dien-7-one(4),15α-acetoxy-(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(5),15β-methoxy-(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(6),along with four known ergosterols(7-10),were *** structures were elucidated based on 1D and 2D NMR spectral *** compounds were evaluated for cytotoxicity against five human cancer cell lines,only compound 4 was found to exhibit a favorable cytotoxicity profile toward all tested tumor cell lines.

关键词： ergosterol Polyporus ellisii basidiomycete cytotoxicity

Hirsutane Sesquiterpenes from Cultures of the basidiomycete Marasmiellus *** 22389

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Natural Products and Bioprospecting 2016年第5期6卷 257-260页

作者： Masahiko Isaka Somporn Palasarn Malipan Sappan Sumalee Supothina Thitiya Boonpratuang National Center for Genetic Engineering and Biotechnology(BIOTEC) 113 Thailand Science ParkPhaholyothin RoadKlongLuangPathumthani 12120Thailand

Two new hirsutane sesquiterpenes,marasmiellins A(1)and B(2),were isolated from cultures of the basidiomycete Marasmiellus *** *** structures were elucidated on the basis of NMR spectroscopic and mass spectrometry ***... 详细信息

关键词： Marasmiellus basidiomycete Hirsutane sesquiterpenoid

The Ability of Edible Mushrooms to Act as Biocatalysts: Preparation of Chiral Alcohols Using basidiomycete Strains

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Advances in Microbiology 2012年第2期2卷 66-71页

作者： Kohji Ishihara Yukiko Nishikawa Mari Kaneko Anna Kinoshita Nozomi Kumazawa Daichi Kobashigawa Kohei Kuroda Masashi Osawa Tatsunori Yamamoto Nobuyoshi Nakajima Hiroki Hamada Noriyoshi Masuoka Department of Life Science Okayama University of Science Okayama Japan Graduate School of Health and Welfare Science Okayama Prefectiral University Soja Japan Research Institute of Biotechnology Fujiwara Techno-Art Co. Ltd. Okayama Japan

To examine the potential ability of edible mushrooms to act as biocatalysts, 19 basidiomycete strains were screened. Modified media (PG, O, and PGO medium) for liquid cultivation of these basidiomycete strains were designed and tested. Wet cells (>10 g) of 4 basidiomycete strains (Pleurotus salmoneostramineus H7, P. salmoneostramineus H13, Ganoderma lucidum NBRC31863, Flammulina velutipes NBRC31862) were harvested from PGO medium for 7 days. The stereoselective reduction of α-keto esters using the 4 strains was tested. It was found that each of these strains had a reducing activity toward 6 aliphatic α-keto esters. In the presence of L-alanine as an additive, the reduction of ethyl 2-oxobutanoate and ethyl 2-oxopentanoete by P. salmoneostramineus H7 produced the corresponding alcohol with a high conversion ratio and with excellent enantiomeric excess (>99% e.e. (R)). Furthermore, ethyl pyruvate, ethyl 2-oxobutanoate, and ethyl 2-oxopentanoate were predominantly reduced to the corresponding (R)-hydroxy ester (>99% e.e.) by G. lucidum. Thus, we found that these edible mushrooms have great potential to be used as biocatalysts for the stereoselective reduction of carbonyl compounds.

关键词： basidiomycete Edible Mushroom Biocatalyst Chiral Alcohol

Bioluminescent fungi from Peninsular Malaysia-a taxonomic and phylogenetic overview

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Fungal Diversity 2015年第1期70卷 149-187页

作者： Audrey L.C.Chew Dennis E.Desjardin Yee-Shin Tan Md Yusoff Musa Vikineswary Sabaratnam Mushroom Research Centre University of Malaya50603 Kuala LumpurMalaysia Institute of Biological Sciences Faculty of ScienceUniversity of Malaya50603 Kuala LumpurMalaysia Department of Biology San Francisco State University1600 Holloway Ave.San FranciscoCA 94132USA

Fifteen bioluminescent fungi recently collected from Peninsular Malaysia are analysed *** phylogeny of the Malaysian bioluminescent fungi and closely related taxa were evaluated with molecular data from the nuclear ribosomal large subunit(LSU),RNA polymerase 2 second largest subunit(RPB2)and internal transcribed spacers(ITS)gene *** sequences data support the circumscription of species based on the morphological species concept,but was unable to fully support the current sectional delimitation of genus *** the 15 taxa analysed here,four are novel species described herein,*** gombakensis,***,*** and Panellus luxfilamentus;while four taxa of Mycena *** were recently described as *** species(***)represents a new distribution record to Peninsular Malaysia,and six species(Neonothopanus nambi,Filoboletus manipularis,***,Roridomyces pruinosoviscidus,*** and ***)have been previously *** pruinosoviscidus is accepted as a new combination based on morphological and phylogenetic *** morphology data indicate its potential for taxon delimitation as axenic cultures of each species produced unique and distinguishable *** descriptions,illustrations and photographs are provided on basidiome and culture morphology.A key to aid in species identification,comparisons with allied species and data on basidiome and mycelium luminescence are also *** study gives the first report on mycelial luminescence for ***,***,*** as well as all new species described *** reports in this study bring the total known luminescent fungi worldwide to 81 species.

关键词： basidiomycete Biodiversity Culture morphology Filoboletus Mycena Neonothopanus Panellus Phylogeny Roridomyces Systematics Taxonomy

Structures and cytotoxicities of three new sesquiterpenes from cultures of Armillaria sp.

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Natural Products and Bioprospecting 2012年第6期2卷 245-248页

作者： Xia YIN Tao FENG Ji-Kai LIU State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China University of Chinese Academy of Sciences Beijing 100049China

Three new sesquiterpene aryl esters,named 10-dehydroxy-melleoliede B(1),1-O-formyl-10-dehydroxy-melleoliede B(2)and 10-oxo-melleoliede B(3)together with six known ones(4-9),were isolated from the cultures of Armillaria *** structures of the new compounds were elucidated based on the extensive spectroscopic *** 1,2,and 5-9 exhibited moderate cytotoxicities.

关键词： basidiomycete Armillaria sp. sesquiterpene aryl ester cytotoxicities

Tetra-,penta-,and hexa-nor-lanostane triterpenes from the medicinal fungus Ganoderma australe

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Natural Products and Bioprospecting 2022年第1期12卷 505-514页

作者： Lin Zhou Subiy Akbar Meng-Xi Wang He-Ping Chen Ji-Kai Liu School of Pharmaceutical Sciences South-Central Minzu University430074 WuhanChina

Chemical investigation on the medicinal fungus Ganoderma australe led to the identification of ten new nor-lanostane triterpenes,namely two hexa-nor ones,ganoaustratetraenones A(1)and B(2),five penta-nor ones,ganoaustraldehydes A-E(3-7),and three tetra-nor ones ganoaustrenoic acids A-C(8-10).The chemical structures along with the absolute configurations were determined by extensive spectroscopic analysis of 1D&2D NMR and HRESIMS *** postulated biosynthesis pathways of these compounds were *** A(3)and B(4)showed moderate inhibition against nitric oxide production in RAW264.7 macrophage cells with the respec-tive IC_(50) values of 32.5,34.2μM(the IC_(50) of positive control pyrrolidine dithiocarbamate was 20.0μM).

关键词： basidiomycete Ganoderma australe Nor-lanostane Structural determination Anti-NO production

Vibralactone Biogenesis-Associated Analogues from Submerged Cultures of the Fungus Boreostereum vibrans

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Natural Products and Bioprospecting 2018年第1期8卷 37-45页

作者： He-Ping Chen Meng-Yuan Jiang Zhen-Zhu Zhao Tao Feng Zheng-Hui Li Ji-Kai Liu School of Pharmaceutical Sciences South-Central University for NationalitiesWuhan 430074People’s Republic of China Key Laboratory of Chemistry in Ethnic Medicinal Resources State Ethnic Affairs Commission&Ministry of EducationSchool of Ethnic MedicineYunnan Minzu UniversityKunming 650504People’s Republic of China

A scale-up fermentation of the fungus Boreostereum vibrans facilitated the isolation of six new vibralactone biogenesis-associated analogues,namely vibralactamide A(1),vibralactone T(2),13-O-lactyl vibralactone(3),10-O-acetyl vibralactone G(4),(11R,12R)-and(11S,12R)-vibradiol(5,6).Their structures were established via extensive spectroscopic analyses,specific optical rotation comparison,and Snatzke’s *** biosynthetic pathway for vibralactamide A was *** absolute configuration of vibralactone B was revised by single crystal X-ray diffraction *** work puts the divergent vibralactone biosynthesis pathway one step further and expands the structural diversity of vibralactone-associated compounds.

关键词： basidiomycete Boreostereum vibrans Vibralactone Structure revision Snatzke’s method

Production of Manganese Peroxidase by Trametes villosa on Unexpensive Substrate and Its Application in the Removal of Lignin from Agricultural Wastes

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Advances in Bioscience and Biotechnology 2014年第14期5卷 1067-1077页

作者： Marilia Lordelo Cardoso Silva Volnei Brito de Souza Veronica da Silva Santos Helio Mitoshi Kamida Joao Ronaldo T.de Vasconcellos-Neto Aristoteles Goes-Neto Maria Gabriela Bello Koblitz Technology Department State University of Feira de Santana(UEFS)Feira de SantanaBrasil Biology Department State University of Feira de Santana(UEFS)Feira de SantanaBrasil Food Technology Department Federal University of the State of Rio de Janeiro(UNIRIO)Rio de JaneiroBrasil

Manganese peroxidase (MnP) is a ligninolytic enzyme that is involved in the removal of lignin from the cell wall of plants. This removal facilitates the access of hydrolytic enzymes to the carbohydrate polymers that are hydrolyzed to simple sugars, which allows the subsequent fermentation to obtain bioproducts, such as ethanol. In this work, response surface methodology (RSM) was employed to optimize the culture conditions on unexpensive substrate for MnP secretion by Trametes villosa. Three independent variables were evaluated (i.e., temperature, moisture content and pH). The crude extract containing MnP was used in the delignification experiment and it caused a reduction in lignin content for all residues tested: 35.05 ± 1.45 (%) for the sugar cane bagasse;63.11 ± 0.06 (%) for the sisal fiber and 39.61 ± 0.39 (%) for the coconut shell, under the reaction conditions tested after 4 hours of fermentation. The preliminary results exhibited the potential application of this enzyme in the removal of lignin from plant residues. However, the conditions should be evaluated and optimized for each residue type.

关键词： Ligninolytic Enzymes basidiomycete Sugarcane Bagasse Coconut Shell Sisal Fiber

Proteins from <i>Punctularia atropurpurascens</i>with Biotechnological Applications

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Natural Resources 2014年第15期5卷 915-925页

作者： Silvana Alborés Maria José Bustamante Laura Franco Fraguas Maria Pía Cerdeiras Cátedra de Microbiología DepBio Facultad de Química Universidad de la República Montevideo Uruguay Cátedra de Bioquímica DepBio Facultad de Química Universidad de la República Montevideo Uruguay

basidiomycetes are able to biodegrade waste and xenobiotic molecules through the production of extracellular enzymes. For example, white-rot fungi produce lignin-degrading enzymes which are capable of efficiently decolorizing dye solutions. Many mushrooms also produce lectins, a group of proteins which bind specifically to the carbohydrates in glycoconjugates. Several fungal lectins target their specificities towards oligosaccharides present in mammalian glycoproteins, thus constituting excellent ligands for the preparation of affinity adsorbents useful in isolation and characterization of these glycoproteins. In this study we isolated and characterized two different proteins, a lectin and a laccase, present in extracts from Punctularia atropurpurascens. The lectin isolated from the mycelium extract, was immobilized on activated-Sepharose and used to evaluate the interaction with three glycoproteins. The adsorbent was able to efficiently adsorb and elute bovine lactoferrin, constituting a promising tool for the purification of this glycoprotein. In vitro experiments revealed that the lectin also exhibited antimicrobial activity against Aspergillus niger. Laccase activity was detected in the extracellular extract from P. atropurpurascens. This enzyme, in both soluble and immobilized forms, was able to degrade Remazol Brilliant Blue R and Acid Blue 25 dyes. The biological activities found in this fungus demonstrate its potential for various biotechnological applications.

关键词： basidiomycete Laccase Decolorization Lectin Glycoproteins