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A β-glucosidase-producing M-2 strain: Isolation from cow dung and fermentation parameter optimization for flaxseed cake

引用

Animal Nutrition 2019年第1期5卷 101-108页

作者： Chenhui Li Junshu Wei Yaping Jing baoxia Teng Pingrong Yang Xinjun Chen Haiying Huang Tang Zhao Tuanjie Che Chunjiang Zhang School of Life Sciences Lanzhou University Key Laboratory of Cell Activities and Stress Adaptations of the Ministry of Education Lanzhou University Gansu Key Laboratory of Functional Genomics and Molecular Diagnosis Gansu Key Laboratory of biomonitoring and bioremediation for Environmental Pollution Lanzhou University Gansu Institute of Drug Control

Flaxseed cake contains cyanogenic glucosides, which can be metabolized into hydrocyanic acid in an animal's body, leading to asphyxia poisoning in cells. beta-glucosidase is highly efficient in degrading cyanogenic glucosides. The Cattle may have b-glucosidase-producing strains in the intestinal tract after eating small amounts of flaxseed cake for a long time. This study aimed to isolate of a strain from cow dung that produces b-glucosidase with high activity and can significantly reduce the amount of cyanogenic glucosides. We used cow dung as the microflora source and an esculin agar as the selective medium. After screening with 0.05% esculin and 0.01% ferric citrate, we isolated 5 strains producing high amounts of b-glucosidase. In vitro flaxseed cake fermentation was fermented by these 5 strains, in which the strain M-2 exerted the best effect(P by a single factor analysis and orthogonal experimental design. The optimum condition was as follows: inoculum size3%, water content 60%, time 144 h, and temperature 32℃. Under this condition, the removal rate of cyanogenic glucosides reached 89%, and crude protein increment reached 44%. These results provided a theoretical basis for the removal of cyanogenic glucosides in flaxseed and the comprehensive utilization of flaxseed cake.

关键词： Flaxseed cake Cyanogenic glycosides Fermentation b-glucosidase Crude protein Lichtheimia

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