检索结果-南通市图书馆

Fidgetin interacting with microtubule end binding protein EB3 affects axonal regrowth in spinal cord injury

Neural Regeneration Research 2023年第12期18卷 2727-2732页

作者： Chao Ma Junpei Wang Qifeng Tu Weijuan Bo Zunlu Hu Run Zhuo Ronghua Wu Zhangji Dong Liang Qiang Yan Liu Mei Liu Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-innovation Center of NeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongJiangsu ProvinceChina Medical School of Nantong University NantongJiangsu ProvinceChina Department of Neurobiology and Anatomy Drexel University College of MedicinePhiladelphiaPAUSA

Fidgetin,a microtubule-severing enzyme,regulates neurite outgrowth,axonal regeneration,and cell migration by trimming off the labile domain of microtubule *** maintenance of the microtubule labile domain is essential for axon initiation,elongation,and navigation,it is of interest to determine whether augmenting the microtubule labile domain via depletion of fidgetin serves as a therapeutic approach to promote axonal regrowth in spinal cord *** this study,we constructed rat models of spinal cord injury and sciatic nerve *** with spinal cord injury,we found that expression level of tyrosinated microtubules in the labile portion of microtubules continuously increased,whereas fidgetin decreased after peripheral nerve *** of fidgetin enhanced axon regeneration after spinal cord injury,whereas expression level of end binding protein 3(EB3)markedly ***,we performed RNA interference to knockdown EB3 or *** found that deletion of EB3 did not change fidgetin ***,deletion of fidgetin markedly increased expression of tyrosinated microtubules and *** of fidgetin increased the amount of EB3 at the end of neurites and thereby increased the level of tyrosinated ***,we deleted EB3 and overexpressed *** found that fidgetin trimmed tyrosinated tubulins by interacting with *** fidgetin was deleted,the labile portion of microtubules was elongated,and as a result the length of axons and number of axon branches were *** findings suggest that fidgetin can be used as a novel therapeutic target to promote axonal regeneration after spinal cord ***,they reveal an innovative mechanism by which fidgetin preferentially severs labile microtubules.

关键词： acetylated microtubules axon regeneration axonal branching axonal regrowth end binding protein 3 fidgetin microtubule dynamics sciatic nerve injury spinal cord injury tyrosinated microtubules

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of PTEN Inhibitor bpv on the axonal regrowth and Functional Recovery of Cerebral Ischemia Reperfusion Injury in Mice

Effect of PTEN Inhibitor bpv on the Axonal Regrowth and Func...

引用

山东省2013年神经内科学学术会议暨中国神经免疫大会2013

作者： Lunlin Mao Jian Cheng Yangtai Guan Department of Neurology Changhai HospitalSecond Military Medical University Institute of Neuroscience Soochow University

Objectives:Study on the effect of PTEN inhibitor bpv on the axonal regrowth and functional recovery of cerebral ischemia reperfusion injury in mice,and explore whether PTEN is the endogenous inhibition mechanism of the axonal regrowth after stroke. Methods:Firstly,according to the modified Zea-Longa suture method,build MCAO-reperfusion injury *** ischemia and reperfusion,at the time point of 1d,3d,7d,14d,Cortical and striatal tissue were harvested from ischemic boundary zones and contralateral separately,use Western-blot technique to detect the p-PTEN and PTEN protein’s expression at each time point of each ***, the animals were randomly divided into 3 groups: Sham group;MCAO + saline group;MCAO+ bpv group,a potent PTEN inhibitor bpv at the doses of 0.2mg/kg/daily via a intra-peritoneal route for 14 days,starting at 24 hours after reperfusion;. MCAO+Saline is injected with the same dose of *** 96h,we use TTC staining and compare each group’s cerebral infarction volume,observe the mortality of animals after 14 days ischemia and the cortical width index at the time point:1d,3d,5d,7d, 9d,14d,we use NSS and forelimb placement test to assess the changes of neurological function in each *** use Bielschowsky Silve staining method to show the distribution of axons within the white matter tracts at the edge of the cerebral ischemia,we use IHC to detect MBP and protein-43’s(GAP-43) expression which is associated with growth on the ischemic border zone. Results:During the animal ischemia reperfusion 1-14 days,total PTEN protein showed persistent high levels of expression in the ischemic cortex and striatum,no significant decrease compared with the Sham;Also no significant changes on cerebral ischemia phosphorylation of PTEN protein’s(non-activated) level compared with the Sham,the levels of phosphorylated and total PTEN protein expression,also its ratio in the contralateral cortex and striatum,compared with the Sham,have no significant chang

关键词： PTEN cerebral ischemia/reperfusion injury axonal regrowth Mice

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

引用

Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui Qu Xiangbing Wu Wei Wu Ying Wang Yan Sun Lingxiao Deng Melissa Walker Chen Chen Heqiao Dai Qi Han Ying Ding Yongzhi Xia George Smith Rui Li Nai-Kui Liu Xiao-Ming Xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Hand Surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals Pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA Department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：