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Asian Pacific Journal of Tropical Biomedicine 2020年第6期10卷 273-280页

作者： Furoida Moolsap Supita Tanasawet Mayuree HTantisira Pilaiwanwadee Hutamekalin Varomyalin Tipmanee Wanida Sukketsiri Department of Pharmacology Faculty of SciencePrince of Songkla UniversitySongkhla90110Thailand Department of Anatomy Faculty of SciencePrince of Songkla UniversitySongkhla90110Thailand Faculty of Pharmaceutical Science Burapha UniversityChon Buri CampusChon Buri20131Thailand Department of Physiology Faculty of SciencePrince of Songkla UniversitySongkhla90110Thailand Department of Biomedical Sciences Faculty of MedicinePrince of Songkla UniversitySongkhla90110Thailand

Objective:To evaluate the effect of standardized extract of Centella asiatica ECa 233 on inflammatory mediator production through cyclooxygenase-2(COX-2),extracellular signal-regulated kinase 1/2(ERK1/2)and nuclear factor-κB(NF-κB)pathway in keratinocyte Ha Ca T ***:Ha Ca T cells were treated with 0.1,1,10 and 100μg/m L ECa 233 in the presence of lipopolysaccharide(LPS).Proinflammatory cytokines and prostaglandin E2 were assessed with *** blotting was used to determine the inhibition of COX-2,ERK1/2 and NF-κB protein ***:ECa 233 suppressed LPS-induced release of interleukin-1β,tumor necrosis factor-α,and prostaglandin *** 233 also inhibited COX-2,phosphorylation of ERK1/2 and the activation of NF-κ***,the formation of reactive oxygen species(ROS)was decreased in response to LPS-inflamed ***:ECa 233 inhibits LPS-stimulated production of inflammatory mediators in keratinocytes via suppressing ERK1/2 and NF-κB *** suppressive effect of ECa 233 may be related to an inhibition of ROS production.

关键词： Centella asiatica Cyclooxygenase-2 HaCaT Interleukin-1β Tumor necrosis factor-α

Antifertility properties ofCentella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic

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Asian pacific Journal of Reproduction 2017年第5期6卷 212-216页

作者： Irfan Yunianto Nurul Ain Kamar Bashah Mahanem Mat Noor School of Biosciences and Biotechnology Faculty of Science and TechnologyUniversiti Kebangsaan Malaysia43600 Bangi SelangorMalaysia

Objective:To investigate the antifertility properties ofCentella asiatica L. ethanolic extract and identify the sperm proteomic changes in treated male ***: A total of 32 male Sprague-Dawley rats were divided into two groups: control group (n=16) received distilled water and treatment group (n=16) which received 300 mg/kg ofCentella asiatica L. extract for 42 d. After the treatment period, the number of implantation sites was recorded and the sperm proteomic changes were analysed by 2D gel electrophoresis. In addition, the expression of protein spots was quantified by MALDI-TOF ***:Centella asiatica L. extract resulted in low number of implantation sites in the treatment group (100.00±2.82) compared to the control group (183.00±2.14). The percentage of infertile male rats in the treatment group was higher (43.75%) compared to the control group (18.75%). Proteomic analysis showed the expression of protein spots identified in the treatment group decreased with 234 spots compared to the control group with 282 ***:The results revealed that there was antifertility activity in the male rats with the administration ofCentella asiatica L. ethanol extract and the identified proteins could provide understanding on the adverse effect ofCentella asiaticain male reproductive system.

关键词： Centella asiatica L Antifertility MALDI-TOF Sperm proteomic

The efficacy of topical spray containing Centella asiatica extract on excision wound healing in rats

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Asian Journal of Pharmaceutical Sciences 2016年第1期11卷 132-133页

作者： Kanuengnit Choochuay Somchai Sawatdee Wirot Chanthorn Drug and Cosmetic Research and Development Unit School of PharmacyWalailak UniversityThasala DistrictNakhon Si Thammarat 80161Thailand Faculty of Public Health Naresuan UniversityTha PhoMuangPhitsanulok 65000Thailand

Centella asiatica (Linn.) belongs to the family of Apiaceae(Umbeliferae). It has been widely used for traditional medicine, especially for wound healing, anti-inflammatory,antipsoriatic, antiulcer, antibacterial and antifungal. The biologically active ingredients of Centella asiatica are believed to be triterpenes, particularly asiatic acid, madecassic acid,asiaticoside and madecassoside (Fig. 1).

关键词： Centella asiatica extract Hydroxypropyl-β-cyclodextrin Wound healing Topical spray

Production of asiaticoside from centella(Centella asiatica ***)cells in bioreactor

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Asian Pacific Journal of Tropical Biomedicine 2013年第10期3卷 806-810页

作者： Nguyen Hoang Loc Nguyen Thi Duy Nhat Institute of Resources.Environment and Biotechnology.Hue University Hue 47000Vietnam

Objective:To investigate the effects of some culture conditions on production of asialicoside from centella(Centella asiatica ***)cells cultured in 5-L ***:The centell cell suspension culture was conducted in 5-L bioreactor to investigate the growth and asiaticoside accumulation under various *** content was determined by HPLC ***:The results showed that the cell growth and asiaticoside accumulation peaked after 24d of culture at an agitation speed of 150 r/min and aeration rate of 2.5 L/*** cell biomass reached a maximum value of 302.45 g fresh weight(31.43 g dry weight)and growth index of 3.03with inoculum size of 100 ***,asiaticoside content was the highest(60.08 mg/g dry weight)when culture was initiated with an inoculum size of 50 ***:The present study found the suitable conditions for growth of centella cells and their asiaticoside production in bioreactor.

关键词： Asiaticoside Bioreactor Centella asiatica Cell suspension culture

Decreasing a-synuclein aggregation by methanolic extract of Centella asiatica in zebrafish Parkinson's model

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Asian Pacific Journal of Tropical Biomedicine 2015年第11期5卷 948-954页

作者： Husnul Khotimah Mulyohadi Ali Sutiman Bambang Sumitro Mochamad Aris Widodo Medical Faculty Brawijaya University Laboratory of Pharmacology Medical FacultyBrawijaya University Department of Biology Faculty of ScienceBrawijaya University

Objective: To observe the effects of Centella asiatica(C. asiatica) methanolic extract on a-synuclein aggregation and its expression in rotenone-exposed zebra ***: Zebra fish(Danio rerio) were exposed to 5 m g/L rotenone for 28 days and coincubated with 2.5, 5.0 and 10.0 m g/mL of C. asiatica methanolic extract. The medium was changed every 48 h for maintain the concentration of rotenone and extract. After 28 days zebra fish were sacrificed on the ice block and protein was isolated from zebra fish brain for ELISA of dopamine and Western blotting of a-synuclein. Immunohistochemistry was conducted to observe the a-synuclein expressions from histopathological preparation of zebra fish brain. The head were soaked in 10% formaline for less than 24 h and embedded onto paraffin block, then sliced for immunohistochemistry using anti a-synuclein antibody. We also measured zebra fish motility for 5 min in each ***: C. asiatica has important bioactive compounds such as asiaticoside that has antiin flammatory and antioxidant properties. It may inhibit cascade reaction due to oxidative stress induced by rotenone. Decreasing reactive oxygen species proposed probability of radical attack to a-synuclein protein that caused aggregation and increase of its *** motility of zebra fish was also maintained in C. asiatica groups due to the increasing dopamine level in rotenone-induced zebra fish. High level of reactive oxygen species inactivated enzyme for dopamine synthesis such as tyrosine hydroxylase, and oxidized dopamine itself. Oxidized dopamine increased a-synuclein aggregation. Thus, the dopamine level decreased in rotenone-induced zebra fish, but C. asiatica increased dopamine ***: C. asiatica has a potential to be developed as an anti-Parkinson's disease treatment due to its capability for minimized the sign of Parkinson's such as a-synuclein aggregation and expression, increasing motility and dopamine as well.

关键词： a-Synuclein Centella asiatica Rotenone Parkinson’s

Enzymatic pretreatment and microwave extraction of asiaticoside from Centella asiatica

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Journal of Biomedical Science and Engineering 2009年第7期2卷 526-531页

作者： Cheng-Xiao Wang Wei Han Liang Fan Chun-Li Wang 不详

The extraction of asiaticoside from Centella asiatica by enzymatic pretreatment and microwave extraction (EPME) was studied in this article. The effects of several important factors such as temperature of enzymatic pretreatment, liquid to solid ratio and microwave radiation time were investigated by quadric regression orthogonal design experiment and were analyzed by response surface. An extraction model with well forecast performance was then established. The results indicate that the optimum extraction condition was as follows: liquid to solid ratio was 36mL/g, temperature of enzymatic pretreatment was 45℃, enzymatic time was 30min, and microwave ra-diation time was

关键词： Asiaticoside Enzymolysis Microwave Extraction Centella asiatica

The Stimulatory Effects of the Antimicrobial Agents Bavistin, Cefotaxime and Kanamycin on In VitroPlant Regeneration of Centella asiatica(L.)—An Important Antijaundice Medicinal Plant

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American Journal of Plant Sciences 2014年第3期5卷 279-285页

作者： Chandra Sekhar Panathula Manikyam Doraiswamy Naidu Mahadev Challagundla Varadarajulu Naidu Department of Biotechnology Dravidian University Kuppam India

Antimicrobial agents such as bavistin, cefotaxime and kanamycin were evaluated for their effects on the rapid shoot regeneration from nodal explants of Centella asiatica (L.). Filter sterilized bavistin (250 mg/L) was augmented alone and in combination with cytokinins such as BAP and TDZ into the media to trace the effect on regeneration. On this basis, the potential use of bavistin (150 mg/L) along with BAP (2.0 mg/L) was evaluated which showed the maximum shoot number (6.6) and shoot length (4.4 cm) respectively. Cefotaxime at the concentration of 100 μM/L was found to be effective to obtain the maximum shoot number formation (5.8) with the regeneration frequency (90%). Kanamycin at the concentration of 80 μM/L induced maximum shoot regeneration (5.12). Kanamycin at 100 μM/L or at higher concentrations reduced the shoot regeneration. The best rooting response was noticed when in vitro regenerated microshoots were transferred to the rooting medim which was supplemented with IBA (2.0 mg/L). This combination generates 90% of regeneration frequency and maximum number of roots per shoot (14.2) and high root length (4.2 cm). The rooted plants were acclimatized and transferred to field for survivalance. The addition of antibiotics was found to be more effective and safer for using since their effects on regeneration were found to be negligible.

关键词： Centella asiatica (L.) Bavistin Cefotaxime Kanamycin 6-Benzyl Amino Purine In Vitro Regeneration

Induced Mutation for Developing Mutant Rice Lines Tolerant to the Parasitic Weed Striga asiatica (L.) Kuntze

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Journal of Environmental Science and Engineering(A) 2019年第5期8卷 181-192页

作者： Noronirina Victorine Rakotoarisoa Harimialimalala Jhonny Rabefiraisana Lydia Razafinirina Berthe Rasoamampionona Xavier Roland Rakotonjanahary Abdelbagi Ali Ghanim Mukhtar Ljupcho Jankuloski Alice Andrianjaka Plant Physiology Laboratory the Department of Plant Biology and Ecology at the Faculty of Sciences-BP:906University of AntananarivoMadagascar Departement de Recherches Rizicoles FOFIFACentre National de Recherche Appliquée au Développement RuralBP:1690101-AntananarivoMadagascar Plant Breeding and Genetics Laboratory Seibersdorf LaboratoryJoint FAO/IAEA ProgrammeIAEA-LaboratoriesA-2444Austria Plant Breeding and Genetics Section Joint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureDepartment of Nuclear Sciences and ApplicationsInternational Atomic Energy AgencyA-1400 ViennaAustria

This work aims to screen mutant rice lines tolerant to Striga *** rainfed sensitive rice varieties B22 and F154 were *** survival rates of the two parents were significantly lower respectively(9.74a and 11.83a)than those of mutant lines(55.36c to 74.36b);Striga plants emergence/pot were significantly higher for the parents(13.96c and14.89c)compared to the mutants(0.12a to 1.5b);the infection rate of parents(7.37b;7.86b)was higher compared to the mutants(2.27a to 2.74a);fertility rate/plant of parents was lower(20.98%b;22.29%b)but much higher than mutants(72.19%b to 78.35%b);the average panicle number/plant of parents was significantly lower(0.5a;1a)than those of mutants(1.5b to 2.4bc)and the 100 g grain weight of parents are lower(2.35a;2.56a)than those of mutants(3.19b to 3.23b).The culture of those mutant lines may increase rice production and contribute to enhancing food security in Madagascar.

关键词： Induced mutation mutant tolerant lines rice (Oryza sativa L.) parasitic weed Striga asiatica (L.) Kuntze.

Content Determination of Total Flavonoids in Tetracera asiatica from 10 Different Production Areas in Guangxi

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Medicinal Plant 2017年第5期8卷 11-13页

作者： Fangfang DA Zhonghua DAI Hua ZHU Guangxi University of Chinese Medicine Chengdu University of Traditional Chinese Medicine

[Objectives] To determine the content of total flavonoids in Tetracera asiatica from different production areas in Guangxi and provide a basis for improving the quality standards of crude drug. [Methods] Using 50% ethanol as extractor,NaNO_2-Al(NO_3)_3-NaOH as chromogenic system and rutin as the reference substance,the content of total flavonoids from the crude drug was determined. [Results] A method for determining the content of total flavonoids was established,the content of total flavonoids was 11.35-21.35 mg·g^(-1),and the average content was 17.64 mg·g^(-1). [Conclusions] The method had high repeatability and stability,and the method was simple,rapid and sensitive and suitable for quantitative analysis.

关键词： Tetracera asiatica Total flavonoids Content determination Guangxi Different production areas

Quantitative Determination of Nitidine from Roots and Plant Tissue Culture Extracts of Toddalia asiatica(Linn.) Using HPTLC

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American Journal of Analytical Chemistry 2014年第2期5卷 65-69页

作者： Chinthala Praveena Ciddi Veeresham University College of Pharmaceutical Sciences Kakatiya University Warangal India

Toddalia asiatica Linn. is an important medicinal plant belonging to the family Rutaceae. The plant is well known for its antimalarial activity, which has been attributed to the presence of benzophenanthridine alkaloid nitidine in the roots of plants. A simple, rapid, sensitive, accurate, repeatable and robust HPTLC method has been developed and validated for the quantitative determination of nitidine in the dried roots and plant tissue culture extracts of T. asiatica. Nitidine was estimated at 332 nm by densitometry using Silica gel 60 F254 as stationary phase and chloroform:methanol (7:1, v/v), and as mobile phase. Linearity was observed in the concentration range of 25 -200 ng/spot for nitidine. The limit of detection and limit of quantitation were found to be 0.026 and 0.086 ng/spot respectively for nitidine. Developed method was validated according to the ICH guidelines with respect to precision, accuracy, specificity and robustness. The technique has been applied for the first time for the estimation of nitidine in roots and plant tissue culture extracts of T. asiatica. Statistical analysis data indicate the accuracy and reliability of the method.

关键词： Nitidine HPTLC Validation Toddalia asiatica