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ascorbate peroxidase 1 confers resistance to southern corn leaf blight in maize

Journal of Integrative Plant Biology 2022年第6期64卷 1196-1211页

作者： Jinghua Zhang Xingmeng Jia Guan-Feng Wang Shijun Ma Shunxi Wang Qin Yang Xueyan Chen Yuqian Zhang Yajing Lyu Xiaoxu Wang Jiawei Shi Yangtao Zhao Yanhui Chen Liuji Wu National Key Laboratory of Wheat and Maize Crop Science Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China The Key Laboratory of Plant Development and Environmental Adaptation Biologym Ministry of EducationSchool of Life SciencesShandong UniversityQingdao 266237China State Key Laboratory of Crop Stress Biology in Arid Areas College of AgronomyNorthwest A&F UniversityYangling 712100China School of Environmental and Rural Science University of New EnglandArmidale 2351NSWAustralia

Southern corn leaf blight (SCLB), caused by Bipolarismaydis, is one of the most devastatingdiseases affecting maize production. However,only one SLCB resistance gene, conferring partialresistance, is currently known, underscoring theimportance of isolating new SCLB resistancerelatedgenes. Here, we performed a comparativeproteomic analysis and identified 258 proteinsshowing differential abundance during the maizeresponse to B. maydis. These proteins included anascorbate peroxidase (Zea mays ascorbate peroxidase1 (ZmAPX1)) encoded by a gene locatedwithin the mapping interval of a previously identifiedquantitative trait locus associated with SCLBresistance. ZmAPX1 overexpression resulted inlower H_(2)O_(2) accumulation and enhanced resistanceagainst B. maydis. Jasmonic acid (JA)contents and transcript levels for JA biosynthesisand responsive genes increased in ZmAPX1-overexpressing plants infected with B. maydis,whereas Zmapx1 mutants showed the oppositeeffects. We further determined that low levels of H_(2)O_(2) are accompanied by an accumulation of JAthat enhances SCLB resistance. These resultsdemonstrate that ZmAPX1 positively regulatesSCLB resistance by decreasing H_(2)O_(2) accumulationand activating the JA-mediated defensesignaling pathway. This study identified ZmAPX1as a potentially useful gene for increasing SCLBresistance. Furthermore, the generated datamay be relevant for clarifying the functions ofplant APXs.

关键词： ascorbate peroxidase comparative proteomics disease resistance jasmonic acid maize southern corn leaf blight

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Mechanisms of Salt Tolerance in Transgenic Arabidopsis thaliana Carrying a Peroxisomal ascorbate peroxidase Gene from Barley

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Pedosphere 2008年第4期18卷 486-495页

作者： XU Wei-Feng SHI Wei-Ming A. UEDA T. TAKABE State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China）. Graduate School of the Chinese Academy of Sciences Beijing 100049 （China） Graduate School of Bioagricultural Sciences Nagoya University Nagoya 464-8601 （Japan

ascorbate peroxidases （APX）, localized in the cytosol, peroxisome, mitochondria, and chloroplasts of plant cells, catalyze the reduction of H2O2 to water by using ascorbic acid as the specific electron donor. To determine the role of peroxisomal type ascorbate peroxidase （pAPX）, an antioxidant enzyme, in protection against salt-induced oxidative stress, transgenic Arabidopsis thaliana plant carrying a pAPX gene （HvAPX1） from barley （Hordeum vulgate L.） was analyzed. The transgenic line pAPX3 was found to be more tolerant to salt stress than the wild type. Irrespective of salt stress, there were no significant differences in Na^＋, K^＋, Ca^2＋, and Mg^2＋ contents and the ratio of K^＋ to Na^＋ between pAPX3 and the wild type. Clearly, the salt tolerance in pAPX3 was not due to the maintenance and reestablishment of cellular ion homeostasis. However, the degree of H2O2 and lipid peroxidation （measured as the levels of malondialdehyde） accumulation under salt stress was higher in the wild type than in pAPX3. The mechanism of salt tolerance in transgenic pAPX3 can thus be explained by reduction of oxidative stress injury. Under all conditions tested, activities of superoxide, glutathione reductase, and catalase were not significantly different between pAPX3 and the wild type. In contrast, the activity of APX was significantly higher in the transgenic plant than in wild type under salt stress. These results suggested that in higher plants, HvAPX1 played an important role in salt tolerance and was a candidate gene for developing salttolerant crop plants.

关键词： ascorbate peroxidase oxidative stress peroxisome soil salinity transgenic Arabidopsis thaliana

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Rapid Inactivation of Chloroplastic ascorbate peroxidase is Responsible for Oxidative Modification to Rubisco in Tomato (Lycopersicon esculentum) under Cadmium Stress

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Journal of Integrative Plant Biology 2008年第4期50卷 415-426页

作者： Kai-Lang Liu Lin Shen Jia-Qi Wang Ji-Ping Sheng College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100094 China

To investigate the sensitive site of antioxidant systems in chloroplast under cadmium stress and its consequence on reactive oxygen species production and action, the sub-organellar localization of chloroplast superoxide dismutases （SOD, EC 1.***.1.1） and ascorbic peroxidase （APX, EC 1.***.1.11） isoenzymes and changes of enzymes activities under cadmium stress were investigated in tomato seedlings. Two APX isoforms, one thylakoid-bound and one stromal, were detected. Cd at 50 μM induced a moderate increase of SOD activities but a rapid inactivation of both APX isoenzymes. APX inactivation was mainly related to the decrease of ascorbate concentration, as supported by in vitro treatment of exogenous ascorbate and APX kinetic properties under Cd stress. H2O2 accumulation in chloroplast, as a consequence of APX inactivation, was associated with a 60% loss of Rubisco （EC 4.***.1.39） activity, which could be partially accounted for by a 10% loss of Rubisco content. Protein oxidation assay found that the Rubisco large subunit was the most prominent carbonylated protein; the level of carbonylated Rubisco large subunit increased fivefold after Cd exposure. Thiol groups in the Rubisco large subunit were oxidized, as indicated by non-reducing electrophoresis. Treating crude extract with H2O2 resulted in a similar pattern of protein oxidation and thiols oxidation with that observed in Cd-treated plants. Our study indicates that APXs in the chloroplast is a highly sensitive site of antioxidant systems under Cd stress, and the inactivation of APX could be mainly responsible for oxidative modification to Rubisco and subsequent decrease in its activity.

关键词： ascorbate peroxidase cadmium hydrogen peroxide protein oxidation Rubisco.

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Influence of heat stress on leaf ultrastructure, photosynthetic performance, and ascorbate peroxidase gene expression of two pear cultivars(Pyrus pyrifolia)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第12期14卷 1070-1083页

作者： Dong-feng LIU Dong ZHANG Guo-qin LIU Sayed HUSSAIN Yuan-wen TENG State Agricultural Ministry Key Laboratory of Horticultural Plant Growth Development and Quality Improvement Department of Horticulture Zhejiang University College of Horticulture Northwest A&F University

Plants encounter a variety of stresses in natural environments. One-year-old pot-grown trees of pear(Pyrus pyrifolia Nakai cv. Cuiguan and Wonhwang) were exposed to two heat stress regimes. Under constant short-term heat stress, chloroplasts and mitochondria were visibly damaged. Relative chlorophyll content and maximum photochemical efficiency of photosystem II were significantly decreased, which indicated that the leaf photosynthetic capability declined. Under chronic heat stress, mesophyll cell ultrastructure was not obviously damaged, but leaf photosynthetic capability was still restrained. As chronic heat stress was a simulation of the natural environment in summer, further study of the responses under this stress regime was undertaken. ascorbate peroxidase(APX) activity was increased in ‘Cuiguan', but not in ‘Wonhwang'. Inducible expression of PpAPX genes in the cytoplasm, chloroplasts and peroxisomes was consistent with increased APX activity in ‘Cuiguan', whereas only weak induction of PpAPX genes was observed in ‘Wonhwang'. The isoenzymes cytosolic APX1(cAPX1) and stromal APX(sAPX) were confirmed to be localized in the cytoplasm and chloroplasts, respectively.

关键词： Pear Ultrastructure ascorbate peroxidase Subcellular localization Synergistic effect

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In Silico Characterization and Homology Modeling of Thylakoidbound ascorbate peroxidase from a Drought Tolerant Wheat Cultivar

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Genomics, Proteomics & Bioinformatics 2009年第4期7卷 185-193页

作者： A. Katiyar S.K. Lenka K. Lakshmi V. Chinnusamy K.C. Bansal National Research Centre on Plant Biotechnology Indian Agricultural Research Institute Department of Botany and Plant Sciences University of CaliforniaRiverside

ascorbate peroxidase, a haem protein （EC 1.***.1.11）, efficiently scavenges hydrogen peroxide （H2O2） in cytosol and chloroplasts of plants. In this study, a fulllength coding sequence of thylakoid-bound ascorbate peroxidase cDNA （TatAPX） was cloned from a drought tolerant wheat cultivar C306. Homology modeling of the TatAPX protein was performed by using the template crystal structure of chloroplastic ascorbate peroxidase from tobacco plant （PDB： IIYN）. The model structure was further refined by molecular mechanics and dynamic methods using various tools such as PROCHECK, ProSA and Verify3D. The predicted model was then tested for docking with H2O2, the substrate for TatAPX enzyme. The results revealed that Arg233 and Glu255 in the predicted active site of the enzyme are two important amino acid residues responsible for strong hydrogen bonding affinity with H2O2, which might play an important role in scavenging of H2O2 from the plant system.

关键词： ascorbate peroxidase wheat molecular modeling docking

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Effects of Catalase and ascorbate peroxidase on the Root Growth of Rice under Cadmium Stress

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Agricultural Science & Technology 2011年第9期12卷 1256-1259页

作者：宋新华王凯郭蕾蕾赵凤云山东理工大学生命科学学院山东淄博255049 淄博市科学技术情报研究所山东淄博255040

[Objective] The research aimed to study the correlations between catalase（CAT） and ascorbate peroxidase（ASP） and the growth and development of rice roots under cadmium stress.[Method] Taking rice variety Zhonghua No.11 as materials,the changes of rice seedlings under the treatment conditions of Cd,Cd＋CAT inhibitor,Cd＋APX inhibitor were studied.[Result] Under Cd stress,inhibition of CAT activity caused the significant inhibition on the growth of aerial parts,decreased the number of adventitious roots and lateral roots,but it can significant promote the elongation growth of adventitious roots and lateral ***,the length of the first lateral root from root tip on the primary roots and adventitious roots was also increased than *** APX activity was inhibited,the growth changes of rice were similar with that treated by CAT inhibitor.[Conclusion] CAT and APX may play important roles in the regulation of rice root system growth in both non-stress and Cd-stressed rice

关键词： Cd stress Catalase ascorbate peroxidase

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Kiwifruit(Actinidia chinensis 'Hongyang') cytosolic ascorbate peroxidases(AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana

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Journal of Integrative Agriculture 2022年第4期21卷 1058-1070页

作者： GUO Xiu-hong HE Yan ZHANG Yu WANG Yi HUANG Sheng-xiong LIU Yong-sheng LI Wei Key Laboratory for Bio-resource and Eco-environment of Ministry of Education College of Life Sciences/State Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064P.R.China School of Horticulture Anhui Agricultural UniversityHefei 230036P.R.China School of Food and Biological Engineering Hefei University of TechnologyHefei 230009P.R.China

ascorbate peroxidase(APX) plays a key role in scavenging reactive oxygen species(ROS) in higher plants. However, there is very little information available on the APXs in kiwifruit(Actinidia), which is an economically and nutritionally important horticultural crop with exceptionally high ascorbic acid(AsA) accumulation. This study aims to identify and characterize two cytosolic APX genes(AcAPX1 and AcAPX2) derived from A. chinensis ‘Hongyang’. The constitutive expression pattern was determined for both AcAPX1 and AcAPX2, and showed relatively higher expression abundances of AcAPX1 in leaf and AcAPX2 in root. Transcript levels of AcAPX1 and AcAPX2 were increased in kiwifruit roots treated with Na Cl. Subcellular localization assays using GFP-fusion proteins in Arabidopsis protoplasts showed that both AcAPX1 and AcAPX2 are targeted to the cytosol. Recombinant AcAPX1 or AcAPX2 proteins were successfully expressed in the prokaryotic expression system and their individual ascorbate peroxidase activities were determined. Finally, constitutive over-expression of AcAPX1 or AcAPX2 could dramatically increase total As A, glutathione level and salinity tolerance under Na Cl stress in Arabidopsis thaliana. Our findings revealed that cytosolic AcAPX1/2 may play an important protective role in the responses to unfavorable environmental stimuli in kiwifruit.

关键词： Actinidia ascorbic acid ascorbate peroxidase salinity

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Differential Expression of Two Cytosolic ascorbate peroxidases and Two Superoxide Dismutase Genes in Response to Abiotic Stress in Rice

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Rice science 2011年第3期18卷 157-166页

作者： Shigeto MORITA Shinya NAKATANI Tomokazu KOSHIBA Takehiro MASUMURA Yasunari OGIHARA Kunisuke TANAKA Laboratory of Genetic Engineering Graduate School of Life and Environmental Sciences Kyoto Prefectural University Basic Research Division Kyoto Prefectural Institute of Agricultural Biotechnology Department of Biological Sciences Tokyo Metropolitan University Kihara Institute for Biological Research Yokohama City University

Superoxide dismutase (SOD) and ascorbate peroxidase (APX) play central roles in the pathway for scavenging reactive oxygen species in plants, thereby contributing to the tolerance against abiotic stress. Here we report the responses of cytosolic SOD (cSOD; sodCc1 and sodCc2) and cytosolic APX (cAPX; OsAPX1 and OsAPX2) genes to oxidative and abiotic stress in rice. RNA blot analyses revealed that methyl viologen treatment caused a more prominent induction of cAPXs compared with cSODs, and hydrogen peroxide treatment induced the expression of cAPXs whereas cSODs were not affected. These results suggest that cAPXs play more important roles in defense against oxidative stress compared with cSODs. It is noted that cSODs and cAPXs showed coordinate response to abscisic acid treatment which induced both sodCc1 and OsAPX2. However, cSODs and cAPXs responded differentially to drought, salt and chilling stress, which indicates that cSOD and cAPX genes are expressed differentially in response to oxidative and abiotic stress in rice.

关键词： ascorbate peroxidase superoxide dismutase reactive oxygen species abiotic stress rice

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Ultraviolet irradiation induced oxidative stress and response of antioxidant system in an intertidal macroalgae Corallina officinalis L.

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Journal of Environmental Sciences 2010年第5期22卷 716-722页

作者： Lixia Li Jiqiang Zhao Xuexi Tang College of Marine Life Science Ocean University of China Qingdao 266003 China Chemistry and Biology College Yan Tai University Yantai 264005 China

The response of the antioxidant defense system of an intertidal macroalgae Corallina officinalis *** different dosages of UV-B irradiation was *** showed that superoxide dimutase （SOD） and peroxidase （POX） increased and then maintained at a relatively stable level when subjected to UV-B *** （CAT） activity under medium dosage of UV-B irradiation （Muv） and high dosage of UV-B irradiation （Huv） treatments were significantly *** peroxidase （APX） activity first remained unaltered and then increased in Huv *** addition,the assay on isozymes was carried out using non-denaturing polyacrylamide gel electrophoresis （PAGE）.The activities of some SOD isoforms were altered by *** new bands （POX V and POX VII） appeared upon exposure to all three UV-B *** III activity was increased by low dosage of UV-B irradiation （Luv）,whereas CAT III and CAT IV disappeared when the alga was exposed to Muv and *** bands of APX （APX VI and APX VII） were increased and a new band （APX X） was observed under Huv exposure.H 2 O 2 and thiobarbituric acid reacting substance （TBARS） increased under Muv and Huv ***,UV-B protection mechanisms are partly inducible and to a certain extent sufficient to prevent the accumulation of damage in ***.

关键词： UV-B irradiation Corallina officinalis L. superoxide dimutase catalase ascorbate peroxidase peroxidase

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Effects of exogenous salicylic acid on growth and H_2O_2-metabolizing enzymes in rice seedlings under lead stress

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Journal of Environmental Sciences 2007年第1期19卷 44-49页

作者： CHEN Jing ZHU Cheng LI Li-ping SUN Zhong-yang PAN Xue-bo State Key Laboratory of Plant Physiology and Biochemistry College of Life Sciences Zhejiang University Hangzhou 310029 China

Salicylic acid （SA） was an essential component of the plant resistance to pathogens and also plays an important role in mediating plant response to some abiotic stress. The possible effects of SA on the growth and H2O2-metabolizing enzymes in rice seedlings under lead stress were studied. When rice seedlings grown in nutrient solution containing Pb^2＋（0, 0.05, 0.15, 0.25 mmol/L） for 18 d, the plant biomass as well as the chlorophyll content of leaves decreased with increasing Pb concentration. The pre-treatment with SA （treated with 0.1 mmol/L SA for 48 h before Pb stress） partially protected seedlings from Pb toxicity. The chlorophyll contents were significant higher in leaves of Pb-exposed with SA pre-treatment seedlings than in Pb-exposed plants at the same Pb intensity. SA pre-treated alone could significantly increase the length of shoot and root of seedlings but the vigour difference was not marked under long-term exposure to Pb toxicity. SA pre-treated influence the H2O2 level in leaves of seedlings by up-regulating the activity of superoxide dismutase （SOD）, repressing the activity of catalase （CAT） and ascorbate peroxidase （APX） depending on the concentrations of Pb^2＋ in the growth medium. The results supported the conclusion that SA played a positive role in rice seedlings against Pb toxicity.

关键词： ascorbate peroxidase catalase H2O2 lead stress rice (Oryza sativa L.) salicylic acid superoxide dismutase

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