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Journal of Analysis and Testing 2023年第2期7卷 128-135页

作者： Ni Zeng Xinyi Wang Yiyang Dong Yan Yang Yingai Yin Lianhui Zhao Xu Wang College of Life Science and Technology Beijing University of Chemical TechnologyBeijing 100029China

Okadaic acid(OA),a small molecule substance derived from shellfish,is one of the most widely distributed marine toxins with acute symptoms of vomiting and diarrhea after accidental *** this,there is an urgently need for sensitive and reliable methods to detect OA in real shellfish *** this study,a simple aptasensor based on screen-printed carbon electrode(SPCE)with modification of chitosan(CS)and gold nanoparticles(Au NPs)was designed for electrochemical determination of OA,and the electrode surface was modified with Au NPs by potential-sweeping electrodeposition,which greatly improved the electrochemical *** entire detection and characterization process were carried out by cyclic voltammetry(CV)with a linear correlation in the range of 0.01-100 ng/m L and a limit of detection(LOD)of 6.7 pg/m ***,recovery rates of 92.3-116%were obtained demonstrating excellent accuracy through the recovery trial of mussel and scallop samples.

关键词： Okadaic acid aptasensor Screen-printed carbon electrode Chitosan Gold nanoparticles Cyclic voltammetry

Electrochemical aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline

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Nano-Micro Letters 2014年第2期6卷 143-152页

作者： Guanghui Shen Yemin Guo Xia Sun Xiangyou Wang School of Agricultural and Food Engineering Shandong University of Technology

In this paper, a novel and sensitive electrochemical aptasensor for detecting tetracycline(TET)with prussian blue(PB) as the label-free signal was fabricated. A PB-chitosan-glutaraldehyde(PB-CS-GA)system acting as the signal indicator was developed to improve the sensitivity of the electrochemical ***, the PB-CS-GA was fixed onto the glass carbon electrode surface. Then, colloidal gold nanoparticles(Au NPs) were droped onto the electrode to immobilize the anti-TET aptamer for preparation of the *** stepwise assembly process of the aptasensor was characterized by cyclic voltammetry(C-V) and scanning electron microscope(SEM). The target TET captured onto the electrode induced the current response of the electrode due to the non-conducting biomoleculars. Under the optimum operating conditions, the response of differential pulse voltammetry(DPV) was used for detecting the concentration of TET. The proposed aptasensor showed a high sensitivity and a wide linear range of 109-～ 105-M and 105-～ 102-M with the correlation coefficients of 0.994 and 0.992, respectively. The detection limit was 3.2×1010-M(RSD 4.12%). Due to its rapidity, sensitivity and low cost, the proposed aptasensor could be used as a pre-scanning method in TET determination for the analysis of livestock products.

关键词： aptasensor Tetracycline Colloidal gold nanoparticle Chitosan

A novel aptasensor strategy for protein detection based on G-quadruplex and exonuclease Ⅲ-aided recycling amplification

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Chinese Chemical Letters 2020年第1期31卷 155-158页

作者： Huan Shi Tian Jin Jiewen Zhang Xiaoting Huang Chunyan Tan Yuyang Jiang Ying Tan State Key Laboratory of Chemical Oncogenomics Key Laboratory of Chemical Biologythe Graduate School at ShenzhenTsinghua UniversityShenzhen 518055China Department of Chemistry Tsinghua UniversityBeijing 100084China Department of Pharmacology and Pharmaceutical Sciences School of MedicineTsinghua UniversityBeijing 100084China

The detection of biomarkers is of great significance in the diagnosis of numerous diseases,especially ***,we developed a sensitive and universal fluorescent aptasensor strategy based on magnetic beads,DNA G-quadruplex,and exonuclease Ⅲ(Exo Ⅲ).In the presence of a target protein,a label-free single strand DNA(ssDNA)hybridized with the aptamer was released as a trigger DNA due to specific recognition between the aptamer and ***,ssDNA initiates the ExoⅢ-aided recycling to amplify the fluorescence signal,which was caused by N-methylmesoporphyrin IX(NMM)insertion into the G-quadruplex *** proposed strategy combines the excellent specificity between the aptamer and target,high sensitivity of the fluorescence signal by G-quadruplex and ExoⅢ-aided recycling *** selected(50-1200 nmol/L)MUC1,a common tumor biomarker,as the proof-of-concept target to test the specificity of our aptasenso *** reveal that the sensor sensitively and selectively detected the target protein with limits of detection(LODs)of 3.68 and 12.83 nmol/L in buffer solution and 10%serum system,*** strategy can be easily applied to other targets by simply substituting corresponding aptamers and has great potential in the diagnosis and monitoring of several diseases.

关键词： aptasensor ExonucleaseⅢ-aided recycling amplification G-quadruplex Magnetic beads MUC1

An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase

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Food Science and Human Wellness 2023年第3期12卷 737-744页

作者： Yanbo Wang Huan Li Jinru Zhou Fangting Wang Yifan Qian Linglin Fu Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China

Simple yet efficient detection methods for food allergens are in urgent need to help people avoid the risks imposed by allergenic *** this work,a polydopamine(PDA)-based fluorescent aptasensor was developed to detect arginine kinase(AK),one of the major allergens in *** aptamer towards AK was firstly selected via systematic evolution of ligands by exponential enrichment method and labeled with fluorescein amidite(FAM)to build a fluorescence resonance energy transfer(FRET)system with PDA *** glycol(PEG)was employed to construct an antifouling surface for the aptasensor to eliminate food matrix *** the presence of AK,the PDA-based aptasensor exhibited elevated fluorescent signals as the FAM-labeled aptamer bound to AK and detached from the PDA *** aptasensor showed great stability and resistance to nonspecific interference of background proteins and had a limit of detection(LOD)of 0.298μg/*** proposed aptasensor was further proved to be feasible for quantitative analysis of AK in nine species of shrimps and five commercial processed products,which indicated its high potential in tracing the presence of AK in complex aquatic products.

关键词： Food allergy aptasensor Antifouling Polydopamine FRET effect

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A Label-free Fluorescent aptasensor for Turn-on Monitoring Ochratoxin A Based on AIE-active Probe and Graphene Oxide

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Chemical Research in Chinese Universities 2018年第3期34卷 363-368页

作者： MA Lian XU Bin LIU Leijing TIAN Wenjing State Key Laboratory of Supramolecular Structure and Materials Jilin University Changehun 130012 P. R. China

A label-free fluorescent aptasensor for specific and ultrasensitive monitoring ochratoxin A（OTA） was developed using the specific aptamer of OTA（OSA） as recognition dement, an aggregation-induced emission（AIE） molecule（a 9,10-distyrylanthracene with two ammonium groups, DSAI） as a fluorescent probe, and graphene oxide（GO） as a quencher. In the absence of OTA, the AIE probe DSAI and OSA complex（DSAI/OSA） is adsorbed on the GO surface, and the fluorescence of DSAI will be quenched efficiently via the fluorescence resonance energy transfer（FRET） from DSAI to GO. Upon the OTA addition, a more stable complex（OSA-OTA） is formed and released from GO. Meanwhile, DSAI and OSA-OTA can form a new complex（DSAI/OSA-OTA）, then the fluorescent signal of DSAI recovers gradually. Therefore, by introducing GO and DSAI, the fluorescence signal of DSAI can be easily turned from ＂off＂ to ＂on＂ after the addition of OTA, and the ultrasensitive detection of OTA by monitoring the change of the fluorescence signal of DSAI can be readily realized. The detection limit of the assay can reach 0.324 nmol/L with a linear detection range of 10-200 nmol/L. And the aptasensor exhibits high selectivity for OTA against other analogues. Moreover, it has been successfully applied for the detection of OTA in red wine samples.

关键词： Label-free aptasensor Aggregation-induced emission(AIE)-active probe Graphene oxide Ochratoxin A

Ultrasensitive and Signal-on Electrochemiluminescence aptasensor Using the Multi-tris(bipyridine)ruthenium(Ⅱ)- β-cyclodextrin Complexes

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Chinese Journal of Chemistry 2014年第11期32卷 1161-1168页

作者： Yingying Zhao Hong Chen Qiong Chen Yantao Qi Fan Yang Jie Tang Pingang He Fan Zhang Department of Chemistry East China Normal UniversityShanghai 200241China

An ultrasensitive and signal-on electrochemiluminescence(ECL)aptasensor to detect target protein(thrombin or lysozyme)was developed using the host-guest recognition between a metallocyclodextrin complex and single-stranded DNA(ss-DNA).The aptasensor uses both the photoactive properties of the metallocyclodextrins named multi-tris(bipyridine)ruthenium(Ⅱ)-β-cyclodextrin complexes and their specific recognition with ss-DNA,which amplified the ECL signal without luminophore *** investigating the ECL performance of different multi-tris(bipyridine)ruthenium(Ⅱ)-β-cyclodextrin(multi-Ru-β-CD)complexes,tris-tris(bipyridine)-ruthenium(Ⅱ)-β-cyclodextrin(tris(bpyRu)-β-CD)was selected as a suitable host molecule to construct an ***,double-stranded DNA(ds-DNA)formed by hybridization of the aptamer and its target DNA was attached to a glassy carbon electrode via coupling interaction,which showed low ECL intensity with 2-(dibutylamino)ethanol(DBAE)as coreactant,because of the weak recognition between ds-DNA and tris(bpyRu)-β-*** addition of the corresponding protein,the ECL intensity increased when target ss-DNA was released because of the higher stability of the aptamer-protein complex than the aptamer-DNA one.A linear relationship was observed in the range of 0.01 pmol/L to 100 pmol/L between ECL intensity and the logarithm of thrombin concentrations with a limited detection of 8.5 fmol/L(S/N=3).Meanwhile,the measured concentration of lysozyme was from 0.05 pmol/L to 500 pmol/L and the detection limit was 33 fmol/L(S/N=3).The investigations of proteins in human serum samples were also performed to demonstrate the validity of detection in real clinical *** simplicity,high sensitivity and specificity of this aptasensor show great promise for practical applications in protein monitoring and disease diagnosis.

关键词： aptasensor host-guest recognition electrochemiluminescence metallocyclodextrin

Fluorescent aptasensor for detection of live foodborne pathogens based on multicolor perovskite-quantum-dot-encoded DNA probes and dual-stirring-bar-assisted signal amplification

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Journal of Pharmaceutical Analysis 2022年第6期12卷 913-922页

作者： Liu Liu Juncheng Hong Wenhai Wang Shu Xiao Hongzhen Xie Qiqin Wang Ning Gan School of Material Science and Chemical Engineering Ningbo UniversityNingboZhejiang315211China Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China

In this study,a fluorescent(FL)aptasensor was developed for on-site detection of live Salmonella typhimurium(S.T.)and Vibrio parahaemolyticus(V.P.).Complementary DNA(cDNA)of aptamer(Apt)-functionalized multicolor polyhedral oligomeric silsesquioxane-perovskite quantum dots(cDNA-POSSPQDs)were used as encoded probes and combined with dual-stirring-bar-assisted signal amplification for pathogen *** this system,bar 1 was labeled with the *** ***,and then bar 2 was functionalized with *** *** *** introduced,pathogen-Apt complexes would form and be released into the supernatant from bar *** agitation,the two complexes reached bar 2 and subsequently reacted with cDNA-POSS-PQDs,which were immobilized on ***,the encoded probes would be detached from bar 2 to generate FL signals in the ***,the pathogens can resume their free state and initiate next *** swim between the two bars,and the FL signals can be gradually enhanced to maximum after several *** FL signals from released encoded probes can be used to detect the *** particular,live pathogens can be distinguished from dead ones by using an *** detection limits and linear range for *** *** 30 and 10 CFU/mL and 10^(2) -10^(6) CFU/mL,***,this assay has broad application potential for simultaneous on-site detection of various live pathogenic bacteria in water.

关键词： aptasensor Live bacteria Multiple detection Multicolor perovskite quantum dots Dual-stirring-bar

Handheld aptasensor for Sandwiched Detection of Chloramphenicol

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Chemical Research in Chinese Universities 2020年第2期36卷 291-295页

作者： YU Mintong XIA Tian BAI Wanchen JI jinyu WANG Huan HUANG Yaqi DENG Shengyuan MA Kefeng SU Yan WAN Ying School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing 210094P.R.China Key Laboratory of New Membrane Materials Ministry of Industry and Information TechnologySchool of Environmental and Biological EngineeringNanjing University of Science and Technology/Nanjing 210094R R.China

Chloramphenicol(CAP)is an antifungal agent approved for use in ***,CAP residue imparts serious adverse effect on human health as well as the ***,keeping track of CAP in aquatic products and its environmental metabolites are of great ***,we developed a novel and neat electrochemical aptasensor for the detection of simulated CAP residual in a sandwich *** screen-printed carbon electrode was employed as the sensing platform and electrodeposited with peculiar nanogolds to enhance the electrochemical *** the specific recognition of such small target,a pair of chloramphenicol-binding aptamers,the primary Aptl and the secondary Apt2,were tailored as an artificial single-chained antibody couple,of which the thi-olated Aptl stood on the coated working substrate to capture the analyte,while the biotinylated Apt2 rallied the signal *** the presence of CAP,Aptl at the lower place cooperated with the upper Apt2 pinched CAP *** that,the reporter avidin-conjugated horseradish peroxidase associated atop the mounted biotin labels,resulting in an electrocatalytic *** whole,this modular biosensing construction would set a paradigm not just for practicable checkpoint quarantine or point-of-care testing,but potentially adaptable for technological fusion with“Internet Plus”and“Internet of Things”.

关键词： Chloramphenicol Screen-printed carbon electrode Electrochemical aptasensor Electrochemical deposi tion Ufkimm

QCM aptasensor for Rapid and Specific Detection of Avian Influenza Virus

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Open Journal of Applied Biosensor 2013年第4期2卷 97-103页

作者： Luke Brockman Ronghui Wang Jacob Lum Yanbin Li Cell and Molecular Biology Program University of Arkansas Fayetteville USA Center of Excellence for Poultry Science University of Arkansas Fayetteville USA Department of Biological and Agricultural Engineering University of Arkansas Fayetteville USA

There has been a need for rapid detection of Avian Influenza virus (AIV) H5N1 due to it being a potential pandemic threat. Most of the current methods, including culture isolation and PCR, are very sensitive and specific but require specialized laboratories and trained personnel in order to complete the tests and are time-consuming. The goal of this study was to design a biosensor that would be able to rapidly detect AIV H5N1 using aptamers as biosensing material and a quartz crystal microbalance (QCM) for transducing method. Specific DNA aptamers against AIV H5N1 were immobilized, through biotin and streptavidin conjugation, onto the gold surface of QCM sensor to capture the target virus. Magnetic nanobeads (150 nm in diameter) were then added as amplifiers considering its large surface/volume ratio which allows for faster movement and a higher target molecule binding rate. The result showed that the captured AIV caused frequency change, and more change was observed when the AIV concentration increased. The nanobead amplification was effective at the lower concentrations of AIV, however, it was not significant when the AIV concentration was 1 HA or higher. The detection limit of the aptasensor was 1 HAU with a detection time of 1 h. The capture of the target virus on to the surface of QCM sensor and the binding of magnetic nanobeads with the virus was confirmed with electron microscopy. Aptamers have unlimited shelf life and are temperature stable which allows this aptasensor to give much more consistent results specifically for in field applications.

关键词： aptasensor Avian Influenza QCM Aptamer Nanobeads

STUDIES ON MICROFLUIDIC CHEMILUMINESCENCE-BASED aptasensor

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STUDIES ON MICROFLUIDIC CHEMILUMINESCENCE-BASED APTASENSOR