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The relationship between serum amyloid A and apolipoprotein a-i in high-density lipoprotein isolated from patients with coronary heart disease

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Chinese Medical Journal 2013年第19期126卷 3656-3661页

作者： WANG Dong-xue LiU Hong YAN Li-rong ZHANG Ye-ping GUAN Xiao-yuan XU Zhi-min JiA You-hong Li Yi-shi zKey Laboratory of Clinical Trial Research in Cardiovascular Drugs Ministry of Health State Key Laboratory of Cardiovascular DiseasesFuwai Hospital National Center for Cardiovascular DiseasesPeking Union Medical College and Chinese Academy of MedicalSciences Beijing 100037China

Background Alteration in the protein composition of high-density lipoprotein （HDL） has been proposed as a mechanism for the development of coronary heart disease （CHD）.in HDL,an increase in serum amyloid A protein （SAA） accompanying the decrease in apolipoprotein a-i （apoA-i） has been found during the acute inflammation ***,whether this phenomenon persists in CHD patients,a disease related to inflammation,is *** purpose of the present study was to explore the relationship between SAA and apoA-i in HDL isolated from CHD *** Overall,98 patients with confirmed stable CHD and 90 control subjects matched for age and gender were enrolled in this case-control *** bromide （KBr） density gradient ultracentrifugation was used to isolate HDL from *** levels of SAA and apoA-i in the HDL samples were detected by enzyme-linked immunosorbent assay ***＇s correlation and general linear models were used in the *** Compared with controls,patients with CHD had a significant decrease in the amount of apoA-i （（14.21±8.44） μg/ml vs.（10.95±5.95） μg/ml,P =0.003） in HDL and a significant increase in the amount of log SAA （1.21±0.46 vs.1.51±0.55,P 〈0.00001）.Differences were independent of age,body mass index （BMi）,HDL cholesterol （HDL-C）,and other *** independently and statistically significant positive correlation between log SAA and apoA-i in HDL was observed only in the CHD group （β =2.0,P =0.026）.in the general linear model,changes in iog（SAA）,age,age2,gender,BMi and HDL-C could explain a statistically significant 43% of the variance in *** This study provides direct evidence for the first time that there was an independent positive correlation between log SAA and apoA-i in the HDL of CHD patients,indicating the alteration of protein composition in ***,the question of whether this alteration in HDL is associated with impairment of HDL functions requires further research.

关键词： coronary heart disease lipoproteins high-density lipoprotein serum amyloid A protein apolipoprotein a-i

Rapid and Facile Purification of apolipoprotein a-i from Human Plasma Using Thermoresponsive Nanoparticles

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Journal of Biomaterials and Nanobiotechnology 2011年第3期2卷 258-266页

作者： Martin Lundqvist Tord Berggård Erik Hellstrand iseult Lynch Kenneth ADawson Sara Linse Tommy Cedervall Centre for BioNano interactions School of Chemistry and Chemical BiologyUniversity College DublinDublinIreland Department of Protein Technology Lund UniversityLundSweden Biophysical Chemistry Lund University Chemical CentreLundSweden

Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein a-i (apoA-i) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-i is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.

关键词： apolipoprotein a-i nanoparticles selective purification

Baseline HDL-C/apolipoprotein a-i ratio predicts the severity of coronary artery lesions in diabetic patients with acute coronary syndrome

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South China Journal of Cardiology 2020年第3期21卷 153-162页

作者： CHEN Guo ZHOU Ying-ling Li Li-wen TAN Hong HE Xu-yu Department of Cardiology Guangdong Cardiovascular InstituteGuangdong Provincial Key Laboratory of Coronary Heart Disease PreventionGuangdong Provincial People's HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China

Background A low high-density lipoprotein cholesterol(HDL-C)to apolipoprotein a-i(apo A-i)ratio which reflects a small HDL-C particle size is emerging as an important predictor of cardiovascular *** study aimed to determine the association of HDL-C/apo A-i ratio with the severity of coronary artery lesions in diabetic *** Observational study was conducted and 478 diabetic patients with acute coronary syndrome(ACS)were *** serum levels of HDL-C,apo A-i,clinical and biochemical parameters were *** patients underwent coronary angiography to evaluate the severity of coronary artery disease(CAD)in terms of the number of stenotic coronary arteries(defined as a stenosis≥50%)and the calculated Gensini *** were then divided into different subgroups according to the two categories:single-,double-or triple-vessel groups;and Gensini Score groups(lower≤4,middle:5-15,and upper≥16).Receiver operating characteristic curves(ROC)were conducted to evaluate the diagnostic values in identifying severe CAD *** association between HDL-C/apo A-i ratio and CAD severity was determined by multivariate logistic regression *** Patients with triple-vessel lesions or upper Gensini score had more CAD risk factors such as older age,smoking,low HDL-C and elevated fasting blood glucose(FBG).A lower HDL-C/apo A-i ratio corresponded to more vessels stenoses and a higher Gensini ***,HDL-C/apo A-i outperformed HDL-C or apo A-i alone in diagnosing severe CAD lesions in ROC ***,multivariate regression analyses revealed that after adjustment for traditional risk factors such as LDL-C,FBG and HAb1c,HDL-C/apo A-i ratio remained independently associated with the severity of CAD in diabetic patients with ACS(all Pions HDL-C/apo A-i may be a useful indicator for the severity of CAD in diabetic patients with ACS.

关键词： high-density lipoprotein cholesterol apolipoprotein a-i coronary artery disease diabetes acute coronary syndrome

Oxidized LDL upregulated ATP binding cassette transporter-1 in THP-1 macrophages

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Acta Pharmacologica Sinica 2004年第5期 39-44页

作者： Chao-ke TANG, Guang-hui Yi, Jun-hao YANG, Lu-shan LiU,Zuo WANG, Chang-geng RUAN, Yong-zong YANG2institute of Cardiovascular Disease of Nanhua University, Hengyang 421001, China

AiM: To study the effect of oxidized low density lipoprotein (ox-LDL) on ATP binding cassette transporter A1(ABCA1) in THP-1 macrophages. METHODS: After exposing the cultured THP-1 macrophages to ox-LDL fordifferent periods, cholesterol efflux was determined by FJ-2107P type liquid scintillator. ABCA1 mRNA and proteinlevel were determined by reverse trancriptase-polymerase chain reaction (RT-PCR) and Western blot, *** cholesterol level in THP-1 macrophage foam cells was detected by high performance liquid ***: ox-LDL elevated ABCA1 in both protein and mRNA levels and increased apolipoprotein (apo) A-i-medi-ated cholesterol efflux in a time- and dose-dependent manner. 22(R)-hydroxycholesterol and 9-cis-retinoic acid didsignificantly increase cholesterol efflux in THP-1 macrophage foam cells (Pively. Both of themfurther promoted cholesterol efflux (Piver X receptor (LXR) agonist decreased content ofesterified cholesterol in the macrophage foam cells compared with control, whereas only a slight decrease of freecholesterol was observed. LXR activity was slightly increased by oxidized LDL by 12 % at 12 h compared with 6 ***, LXR activity was increased about 1.8 times at 24 h, and oxidized LDL further increased LXR activity byabout 2.6 times at 48 h. CONCLUSiON: ABCA1 gene expression was markedly increased in cholesterol-loadedcells as a result of activation of LXR/RXR. ABCA1 plays an important role in the homeostasis of cholesterol in themacrophages.

关键词： ATP-binding cassette transporters apolipoprotein a-i hydroxycholesterols LDL lipoproteins oxi-dation-reduction macrophages

High-density lipoprotein associated factors apoA-i and serum amyloid A in Chinese non-diabetic patients with coronary heart disease

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Chinese Medical Journal 2010年第6期123卷 658-663页

作者： CHEN Guo-liang LiU Li-wei XiE Shuang LiU Hong LiU Yu-qing Li Yi-shi Key Laboratory of Clinical Trial Research in Cardiovascular Drug Ministry of HealthCardiovascular Institute and Fu Wai HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100037China

Background High-density lipoprotein cholesterol（HDL-C）levels are a strong,independent inverse predictor of coronary heart disease （CHD）.in this cross-sectional study we investigated the interrelationships between HDL-C and HDL relaled factors apolipoprotein a-i（apoA-i）and serum amyloid A（SAA）and the presence and extent of CHD in a population of Chinese patients with CHD. Methods Two hundred and twenty-four consecutive patients took part in this *** data were obtained from hospital *** chemical concentrations were measured by standard laboratory *** The concentrations of high-sensitive C-reactive protein（hsCRP）（median：1.85 mg/L）and SAP,（median：9.40 mg/L）were significantly higher in the CHD group（P〈0.05）,while concentrations of HDL-C（0.03±0.25）mmol/L）and apoA-i（（604.59±1 05.79）mmol/L）were significantly lower than those in the non-CHD group（P〈0.05）.The concentrations of apoA-l decreased with the increase in vascular *** the difference did not reach statistical ***, the concentrations of hsCRP and SAA increased with the increase in vascular *** unadjusted odd ratios（ORs）（Ci） for apoA-i and SAA of the presence of CHD were 0.093（0.990-0.997）（P=0.00）and 2.571（1.029-6.424）（P〈0.05）,*** association between elevated SAA and the presence of CHD was lost after adjusting for lipid status parameter *** associations between *** and the extent of CHD remained strong,regardless of confounding *** increased concentrations of SAA represent the inflammatory marker of the extent of coronary stenosis in patients with *** contrast to SAA, the level of apoA-i was also associated with the presence of CHD, indicating that apoA-i was not only a marker of CHD presence but also a quantitative indicator of CHD *** *** the change apolipoprotein content within HDL particle is a more accurate and effective method to evaluate the impact of HDL on CHD.

关键词： high-density lipoprotein apolipoprotein a-i serum amyloid A coronary heart disease

Ecto-F_1-ATPase: A moonlighting protein complex and an unexpected apoA-i receptor

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World Journal of Gastroenterology 2010年第47期16卷 5925-5935页

作者： Pierre Vantourout Claudia Radojkovic Laeticia Lichtenstein Véronique Pons Eric Champagne Laurent O Martinez institut national de la santé et de la recherche médicale U1048 institute of Metabolic and Cardiovascular Diseases Université Toulouse III Paul-Sabatier

Mitochondrial ATP synthase has been recently detected at the surface of different cell types, where it is a high affinity receptor for apoA-i, the major protein component in high density lipoproteins (HDL). Cell surface ATP synthase (namely ecto-F1-ATPase) expression is related to different biological effects, such as regulation of HDL uptake by hepatocytes, endothelial cell proliferation or antitumor activity of Vγ9/Vδ2 T lymphocytes. This paper reviews the recently discovered functions and regulations of ecto-F1-ATPase. Particularly, the role of the F1-ATPase pathway(s) in HDL-cholesterol uptake and apoA-imediated endothelial protection suggests its potential importance in reverse cholesterol transport and its regulation might represent a potential therapeutic target for HDL-related therapy for cardiovascular diseases. Therefore, it is timely for us to better understand how this ecto-enzyme and downstream pathways are regulated and to develop pharmacologic interventions.

关键词： F1Fo ATP synthase High density lipoproteins receptor apolipoprotein a-i Purinergic receptor P2Y13 Adenylate kinase Nucleotide Endothelium Antitumor immunity

Diagnostic accuracy of serum biochemical fibrosis markers in children with chronic hepatitis B evaluated by receiver operating characteristics analysis

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World Journal of Gastroenterology 2005年第45期11卷 7192-7196页

作者： Dariusz Marek Lebensztejn Elzbieta Skiba Jolanta Tobolczyk Maria Elzbieta Sobaniec-Lotowska Maciej Kaczmarski Ⅲrd Department of Pediatrics Department of Children Allergology Department of Clinical Pathomorphology

METHODS： We determined the serum level of apolipoprotein a-i （APO A-i）, haptoglobin （HPT） and a-2 macroglobulin （A2i） with an automatic nephelometer in 63 children （age range 4-17 years, mean 10 years） with biopsy-verified chronic HBeAg-positive hepatitis B. Fibrosis stage and inflammation grade were assessed in a blinded fashion according to Batts and Ludwig. We defined mild liver fibrosis as a score ≤2 and advanced fibrosis as a score equal to 3. ROC analysis was used to calculate the power of the assays to detect advanced liver fibrosis （AccuROC, Canada）. RESULTS： Serum concentrations of APO A-i, HPT and A2M were not significantly different in patients with chronic hepatitis B compared to controls. However, APO A-i level of 1.19 ng/L had a sensitivity of 85.7% and a specificity of 60.7% （AUC = 0.7117, P = 0.035） to predict advanced fibrosis. All other serum biochemical markers and their combination did not allow a useful prediction. None of these markers was a good predictor of histologic inflammation. CONCLUSiON： apolipoprotein a-i may be a suitable serum marker to predict advanced liver fibrosis in children with chronic hepatitis B.

关键词： Chronic hepatitis B Liver fibrosis Children apolipoprotein a-i Haptoglobin a-2 macroglobulin

仿生型纳米载体介导的靶向siRNA递送系统的研究(英文)

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仿生型纳米载体介导的靶向siRNA递送系统的研究(英文)