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Health 2021年第3期13卷 299-305页

作者： Shiho Morishita Shigeru Watanabe Kei Miyazawa Iwai Tohnai Department of Oral Health Sciences Meikai University School of Health Sciences Akemi 1 Urayasu Chiba Japan

Purpose: To investigate the effect of silver ionised water on acid production in plaque. Methods: After injecting 0.5 mL of silver ionised water (concentration: 5 ppm) produced with the sintering coating method in the sensor part of the pH metre, plaque collected from the oral cavity by one platinum loop was mixed in. Immediately after that, a 5% sucrose solution (1 g/20 mL) was added and the pH was continuously measured for 30 minutes at 1-minute intervals (A). Similarly, silver ionised water was mixed with 5% sucrose solution in the same way as in (A) at 3 (B), 5 (C), 10 (D) and 15 minutes (E) after plaque contamination. The pH was measured at 1-minute intervals. The pH of the purified water containing no silver ionised water mixed with plaque and sugar solution at the same time was measured and used as a control. Each experiment was conducted three times, and the pH measured every minute was compared as a percentage of the pH at the beginning of the measurement (100%). Results: Analysis of variance of the repeated measurements to determine the effect of silver ionised water on the decrease in pH revealed a main effect of silver ionised water and an interaction between time and group [F(1.302,20.826) = 39.145, p Conclusion: Silver ionised water was found to inhibit the acid production in plaque.

关键词： Silver Ionised Water antimicrobial effect Plaque Stephan Curve pH

Study on the antimicrobial effect of the Extracts of Clerodendron bungei Roots

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Agricultural Science & Technology 2009年第5期10卷 130-133页

作者：林娜尹礼国陈超众魏琴李华兰宜宾学院生命科学与食品工程系四川宜宾644000

[ Objective ] The aim was to research the antimicrobial effect of the extracts of Clerodendron bungei roots with different solvents on the common plant microbes. [ Method] The bacteria cake method was used to study the antimicrobial capability of Clerodendron bungei roots. [ Result ] The extracts have anti-bacterial action on Rhixoctonia cerealis V. Hoeven, Fusarium graminearum, Rhizoctonia solani and Setosphaeria turcica, especially the extract from distilled water has the strongest anti-bacterial action on Rhixoctonia cerealis V. Hoeven and Fusarium graminearum, the extract from ether has the strongest anti-bacterial action on Fusarium graminearum, the MIC all were 10 mg/ml. The extracts had different antimicrobial effect when pH value was from 5 to 8[ Conclusion] The extracts of Clerodendron bungei roots could inhibit the 4 pathogenic microorganism, and the antibacterial activity was affected by pH value greatly.

关键词： Clerodendron bungei roots Extracts antimicrobial effect

antimicrobial and Photocatalytic effect of Silicate and Silicone Hygienic Coatings

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Journal of Agricultural Science and Technology 2011年第1期5卷 57-62页

作者： J. Vytrasova P. Snevajsova S. Klechova L. Hochmannova Department of Biological and Biochemical Sciences Faculty of Chemical Technology University of Pardubice Studentska 573Pardubice 53210 Czech Republic Department of Analytical Chemistry Faculty of Chemical Technology University of Pardubice Studentska 573 Pardubice 53210Czech Republic SYNPO a.s. S K. Neumanna 1316 Pardubice 53207 Czech Republic

antimicrobial and photocatalytic effect of silicate and silicone paints were tested. Silicate paints are based on water styrene-acrylic dispersion with mineral binder into form of potassium water glass. Silicone paints are based on water acrylic dispersion with silicone resin emulsion. Silicate and silicone paints were formulated with photocatalytic active nanooxides TiO2 and ZnO. Photocatalytic efficiency of coatings was measured as an absorbance change of organic dye Orange II solution at a wavelength of 485 nm. antimicrobial properties of coatings were tested using agar plate methods. As test microorganisms Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Penicillium chrysogenum and Aspergillus niger suspension （density 10^6 cells/mL） were used. The inhibition effect of tested coatings and inhibitory zones were evaluated after incubation. Coatings containing nanoparticles of titanium dioxide and zinc oxide showed antimicrobial activity against the test microorganisms. Whereas coating with zinc oxide nanoparticles successfully inhibited the growth of both bacteria and fungi, the photocatalytic nanoparticles of titanium dioxide inhibited bacteria but not fungi （weak inhibition was observed）.

关键词： Hygienic coatings antimicrobial effect photocatalytic effect.

Synthesis and Characterization of Green Potassium Nanoparticles from Sideroxylon Capiri and Evaluation of Their Potential antimicrobial

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Journal of Renewable Materials 2021年第10期9卷 1699-1706页

作者： Judith Constantino-Alcazar Miguel Abud-Archila Benjamín Valdez-Salas Federico Gutierrez-Miceli Carlos Ceceña-Duran Blanca López-Valenzuela Daniel Gonzalez-Mendoza Tecnológico Nacional de México Instituto Tecnológico de Tuxtla GutiérrezTuxtlaGutiérrezC.P.03940México Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California(ICA-UABC) Ejido Nuevo LeónC.P.21705México Instituto de Ingeniería de la Universidad Autónoma de Baja California Mexicali21100México

In the present study,the green synthesis of potassium nanoparticles(K-NPs)was assessed using aqueous extract of Sideroxylon *** potassium nanoparticles were analyzed by UV-visible spectroscopic techniques,X-ray spectrometers of energy dispersive(SEM-EDS)and dynamic light *** results showed high values at 3.5 keV confirming the formation of potassium nanoparticles and the SEM analysis showed an agglomerated particles size between 360 to 200 nm with a spherical *** K-NPs showed an effective antibacterial activity against the test organisms mainly with Bacillus cereus,Enterobacter aerogenes,Fusarium solani and Botrytis *** further studies about nanotoxicity of K-NPs are needed to confirm their potential in the control of the pathogen microorganisms under field conditions.

关键词： antimicrobial effect green synthesis Sideroxylon capiri biocontrol

Review of the potential of mesenchymal stem cells for the treatment of infectious diseases

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World Journal of Stem Cells 2021年第6期13卷 568-593页

作者： Amit Sharma Anuja Chakraborty Bithiah Grace Jaganathan Stem Cell and Cancer Biology Group Department of Biosciences and BioengineeringIndian Institute of Technology GuwahatiGuwahati 781039India

The therapeutic value of mesenchymal stem cells (MSCs) for the treatment of infectious diseases and the repair of disease-induced tissue damage has been explored extensively. MSCs inhibit inflammation, reduce pathogen load and tissue damage encountered during infectious diseases through the secretion of antimicrobial factors for pathogen clearance and they phagocytose certain bacteria themselves. MSCs dampen tissue damage during infection by downregulating thelevels of pro-inflammatory cytokines, and inhibiting the excessive recruitment ofneutrophils and proliferation of T cells at the site of injury. MSCs aid in theregeneration of damaged tissue by differentiating into the damaged cell types orby releasing paracrine factors that direct tissue regeneration, differentiation, andwound healing. In this review, we discuss in detail the various mechanisms bywhich MSCs help combat pathogens, tissue damage associated with infectiousdiseases, and challenges in utilizing MSCs for therapy.

关键词： Infectious diseases Mesenchymal stem cells antimicrobial effect Immunomodulation Tissue repair COVID-19

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Physicochemical characteristics,GABA content,antimicrobial and antioxidant capacities of yogurt from Murrah buffalo milk with different fat contents

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Food Bioscience 2022年第5期49卷 792-798页

作者： Aliah Zannierah Mohsin Anis Asyila Marzlan Belal J.Muhialdin Lew Kah Wai Nameer Khairullah Mohammed Anis Shobirin Meor Hussin Faculty of Food Science and Technology Universiti Putra Malaysia43400UPM SerdangSelangorMalaysia Halal Products Research Institute Universiti Putra Malaysia43400UPM SerdangSelangorMalaysia Faculty of Agriculture University of TikritTikritIraq Department of Food Science and Nutrition University of Minnesota1334 Eckles AveSaint PaulMN55108USA

This study aimed to prepare yogurt from Murrah buffalo milk with high GABA content and low fat content while keeping the physicochemical characteristics,antimicrobial effects,and antioxidant capacity within acceptable *** samples were prepared using buffalo milk by adjusting the fat content to 0.2%,3.5%,and 6.5%.The results showed that the fat content had no significant effects on the pH,titratable acidity,water activity,and cell ***,the fat content significantly affected the viscosity,particle size,and texture profile analysis of the *** GABA content was significantly higher in the 0.2%fat yogurt(61.57±3.22 mmol L^(-1))compared to the 3.5%fat yogurt(43.64±1.99 mmol L^(-1))and 6.5%fat yogurt(27.84±2.64 mmol L^(-1)).Glutamic acid and proline were the most abundant amino acids in the *** contrast,saturated fatty acids(butyric,lauric,myristic,palmitic,stearic,and oleic)and linoleic acid were *** with 3.5%fat showed significantly high antioxidant capacity and antimicrobial effects on *** O157 and Salmonella *** on the results,fat content significantly affects the rheological properties,GABA content,and antioxidant and antimicrobial capacities of *** the future,3.5%fat should be chosen as the optimized formula for large-scale yogurt production from Murrah buffalo milk.

关键词： antimicrobial effect Antioxidant capacity Buffalo milk Yogurt GABA

effectiveness of Disinfection of Anesthetics Tubes in Oral Surgery -An in Vitro Study

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Journal of Pharmacy and Pharmacology 2017年第7期5卷 424-429页

作者： José Kayque Neves Mayke Felipp de Araújo Martins Josefa Elaine Silva Germinio Maria Cristina de ndrade Sibele Ribeiro de Oliveira Dentistry course Research Group on Pathology of Communicable and Non-Communicable Diseases (GPPATO) Centro Universitário Tabosa de Almeida/Asces-Unita University district Caruaru/Pernambuco 55016-901 Brazil Biomedicine course Research Group on Pathology of Communicable and Non-Communicable Diseases (GPPATO) Centro Universitário Tabosa de Almeida/Asces-Unita University district Caruaru/Pernambuco 55016-901 Brazil

Context： Anesthetic tubes are always on the surgical table, for this reason, it＇s necessary to perform its disinfection, generally by chemicals. Aim： Was to analyze the effectiveness of tubes decontamination made by disinfectant substances used in a clinical setting and to identify the microorganisms found on their surface. Materials and Methods： 14 anesthetic tubes were collected from dental clinic, on 5 groups. 01 plastic and 01 glass tubes that were not submitted to any disinfection process composed the control group and they had been sown in Agar Blood. Experimental groups underwent immersion and fixation with the proposed chemicals for 1 minute, laminar flow hood was used to seed it in Agar Blood and then it was transferred to incubator for 24 hours at 37℃. Tests were used to identify the main Gram positive pathogenic genera. Gram-negative pathogenic colonies isolated were seeded in biochemical means of identification. Results： It was possible to identify the presence of Staphylococcus coagulase negative, Staphylococcus aureus, Streptococcus viridans, Staphylococcus saprophyticus, Escherichia coli and Acinetobacter spp, as well as the effectiveness of chemical solutions proposed in this study. Conclusions： All chemical solutions proposed in this study were effective for the surface disinfection process of anesthetic tubes.

关键词： Anesthetics antimicrobial effect disinfection oral surgery microbiology.

Copper as an alternative antimicrobial coating for implants-An in vitro study

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World Journal of Transplantation 2017年第3期7卷 193-202页

作者： Claudia Bergemann Sarah Zaatreh Katharina Wegner Kathleen Arndt Andreas Podbielski Rainer Bader Cornelia Prinz Ulrich Lembke J Barbara Nebe Department of Cell Biology University Medical Center Rostock Biomechanics and Implant Technology Research Laboratory University Medical Center Rostock Institute of Medical Microbiology Virology and HygieneUniversity Medical Center Rostock DOT GmbH

AIM To investigate osteoconductive and antimicrobial properties of a titanium-copper-nitride(TiC uN) film and an additional BONIT~? coating on titanium *** For micro-structuring, the surface of titanium test samples was modified by titanium plasma spray(TPS). On the TPS-coated samples, the Ti Cu N layer was deposited by physical vapor deposition. The BONIT~? layer was coated electrochemically. The concentration of copper ions released from TiC uN films was measured by atomic absorption spectrometry. MG-63 osteoblasts on Ti Cu N and BONIT~? were analyzed for cell adhesion, viability and spreading. In parallel, Staphylococcus epidermidis(S. epidermidis) were cultivated on the samples and planktonic and biofilm-bound bacteria were quantified bycounting of the colony-forming units. RESULTS Field emission scanning electron microscopy(FESEM) revealed rough surfaces for TPS and TiC uN and a special crystalline surface structure on TiC uN + BONIT~?. TiC uN released high amounts of copper quickly within 24 h. These release dynamics were accompanied by complete growth inhibition of bacteria and after 2 d, no planktonic or adherent S. epidermidis were found on these samples. On the other hand viability of MG-63 cells was impaired during direct cultivation on the samples within 24 h. However, high cell colonization could be found after a 24 h pre-incubation step in cell culture medium simulating the in vivo dynamics closer. On pre-incubated TiC uN, the osteoblasts span the ridges and demonstrate a flattened, well-spread phenotype. The additional BONIT~?-coating reduced the copper release of the TiC uN layer significantly and showed a positive effect on the initial cell *** The Ti Cu N-coating inhibits the formation of bacterial biofilms on orthopedic implants by influencing the "race for the surface" to the advantage of osteoblasts.

关键词： Implant-coating antimicrobial effect Titanium plasma spray Titanium-copper-nitride BONIT^(®) Osteoconductivity

A green synthesis of antimicrobial compounds from marine microalgae Nannochloropsis oculata

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Journal of Coastal Life Medicine 2014年第11期 859-863页

作者： Duraiarasan Surendhiran Mani Vijay Abdul Razack Sirajunnisa Thiruvengadam Subramaniyan Ammavasai Shanthalin Shellomith Kuppusamy Tamilselvam Bioelectrochemical Laboratory Department of Chemical EngineeringAnnamalai UniversityAnnamalai NagarTamilnadu-608002India

Objective:To determine the antibacterial and anti-candidal activities of fatty acid methyl esters(FAME)extracted from marine microalga Nannochloropsis oculata and evaluate the inhibition activity of DNA isolated from test pathogenic ***:FAME was synthesized by transesterification of oil using immobilized lipase and characterized using gas chromatography-mass *** FAME profile was determined using gas *** antimicrobial effect was tested by disc diffusion method against Gram-positive bacteria Staphylococcus aureus,Bacillus subtilis,Gram-negative bacteria Escherichia coli,Pseudomonas aeruginosa and yeast Candida albicans,at varying concentrations of 10,20 and 30μL/***:The results shown that palmitic acid(C16:0),oleic acid(C18:1)and arachidic acid(C20:0)were dominant in Nannochloropsis oculata *** study revealed that FAME was more active against Gram-negative than against Gram-positive and *** inhibition activity results also confirmed that FAME had the bactericidal effect that was revealed by sheared fragments of ***:The results indicated that microalgal FAME could be potentially utilized as a newer and good source of therapeutic agent in pharmaceutical industry.

关键词： Nannochloropsis oculata FAME antimicrobial effect Zone of inhibition Gas chromatography-mass spectrometer