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An Indirect ELISA of Classical Swine Fever Virus Based on Quadruple antigenic epitope Peptide Expressed in ***

Virologica Sinica 2010年第1期25卷 71-76页

作者： Guo-zhen LIN Fu-ying ZHENG Ji-zhang ZHOU Xiao-an CAO Xiao-wei GONG Guang-hua WANG Chang-qing QIU Key Laboratories of Grazing Animal Diseases and Animal Virology Ministry of Agriculture Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China State Key Laboratory of Veterinao Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China

In this study,a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV)E2 glycoprotein was expressed in *** to a obtain target *** target protein was used as a coating antigen to establish an indirect ELISA for specifically detecting anti-CSFV antibodies in serum samples from *** P/N cut-off value of this assay was 1.92 by receiver operating characteristic curve(ROC)analysis based on 30 negative sera and 80 positive *** test gave 97.5%sensitivity and 96.7%specificity compared with the indirect hemagglutination(IHA)*** inter-assay and intra-assay coefficients of variation (CVs)for 16 sera were both≤6.8%.No cross-reactivity between the coating antigen and anti-bovine viral diarrhoea virus(BVDV)antibodies was observed.

关键词： antigenic epitope Bovine viral diarrhoea virus (BVDV) Classical swine fever virus (CSFV) Expression Indirect ELISA

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Computational analysis of antigenic epitopes of avian influenza A (H7N9) viruses

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Science China(Life Sciences) 2015年第7期58卷 687-693页

作者： LIU Mi SONG TingRui HUA Sha WU AiPing JIANG TaiJiao Institute of Biophysics Chinese Academy of Sciences University of Chinese Academy of Sciences

Influenza virus can rapidly change its antigenicity, via mutation in the hemagglutinin(HA) protein, to evade host immunity. The emergence of the novel human-infecting avian H7N9 virus in China has caused widespread concern. However, evolution of the antigenicity of this virus is not well understood. Here, we inferred the antigenic epitopes of the HA protein from all H7 viruses, based on the five well-characterized HA epitopes of the human H3N2 virus. By comparing the two major H7 phylogenetic lineages, i.e., the Eurasian lineage and the North American lineage, we found that epitopes A and B are more frequently mutated in the Eurasian lineage, while epitopes B and C are more frequently mutated in the North American lineage. Furthermore, we found that the novel H7N9 virus(derived from the Eurasian lineage) isolated in China in the year 2013, contains six frequently mutated sites on epitopes that include site 135, which is located in the receptor binding domain. This indicates that the novel H7N9 virus that infects human may already have been subjected to gradual immune pressure and receptor-binding variation. Our results not only provide insights into the antigenic evolution of the H7 virus but may also help in the selection of suitable vaccine strains.

关键词： antigenic epitope antigenic evolution hemagglutinin influenza H7N9 virus

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