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Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第4期10卷 264-272页

作者： Jing LI Qian YANG Li-hua ZHAO Shu-mei ZHANG Yu-xia WANG Xiao-yu ZHAO Department of Life Science and Engineering Harbin Institute of Technology Harbin 150001 China Institute of Microbiology of Heilongjiang Academy of Sciences Harbin 150010 China Heilongjiang University Press Harbin 150040 China

An antifungal protein was isolated from a culture of Bacillus subtilis strain B29. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl （DEAE）-52 cellulose and gel filtration chromatography on Bio-Gel P-100. The protein was absorbed on DEAE-cellulose and Bio-Gel P-100. The purified antifungal fraction was designated as B29I, with a molecular mass of 42.3 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）, pl value 5.69 by isoelectric focusing （IEF）-PAGE, and 97.81% purity by high performance liquid chromatography （HPLC）. B291 exhibited inhibitory activity on mycelial growth in Fusarium oxysporum, Rhizoctonia solani, Fusarium moniliforme, and Sclerotinia sclerotiorum. The 50% inhibitory concentrations （IC50） of its antifungal activity toward Fusarium oxysporum and Rhizoctonia solani were 45 and 112 μmol/L, respectively. B29I also demonstrated an inhibitory effect on conidial spore germination of Fusariurn oxysporum and suppression of germ-tube elongation, and induced distortion, tumescence, and rupture of a portion of the germinated spores.

关键词： Bacillus subtilis antifungal protein Purification

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The Biocontrol Effects of the Bacillus licheniformis W10 Strain and Its antifungal protein Against Brown Rot in Peach

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Horticultural Plant Journal 2015年第3期1卷 131-138页

作者： JI Zhaolin HE Huiwen ZHOU Huijuan HAN Feng TONG Yunhui YE Zhengwen XU Jingyou College of Horticulture and Plant Protection Yangzhou University Forestry and Fruit Research Institute Shanghai Academy of Agricultural Sciences

The biocontrol effects of Bacillus licheniformis W10 bacterial suspension and its antifungal protein on peach brown rot caused by Monilinia fructicola in storage peach fruits and the effects on fruit quality were investigated. The results showed that the fruit disease suppression of B. licheniformis W10 bacterial suspension and antifungal protein were significantly higher than that of the control. Inoculation of bacterial suspension and antifungal protein prior to M. fructicola gave a better biocontrol effect, and the higher concentrations of bacterial(1 × 1010 cfu · m L-1) and antifungal protein(3.0 mg · m L-1) performed better control effects. The environmental conditions, such as temperature and humidity, affected biocontrol effects of W10 bacterial suspension and antifungal protein. The influence of environment conditions on the activity of antifungal protein was less than that on bacterial suspension. Moreover, lower temperature(4 ℃) and relative humidity(RH 70%–75%) were favorable to prevent peach brown rot by W10 bacterial suspension and its antifungal protein. The W10 bacterial suspension and antifungal protein amended with calcium [0.1% Ca(NO3)2] could enhance the biocontrol effects, and obviously put off the occurrence of peach brown rot. In addition, the bacterial suspension and antifungal protein significantly reduced the natural decay rates of peach fruits during storage, and the effects were equal to carbendazim. Moreover, both W10 bacterial suspension and antifungal protein treatments did not have effects on external and internal fruit appearance, such as chromatic aberration parameter L* of flesh, flesh firmness, soluble solids content and weight loss. Therefore, the B. licheniformis W10 is a potential biocontrol factor for peach brown rot.

关键词： peach Bacillus licheniformis antifungal protein Monilinia fructicola biological control fruit quality

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A Novel antifungal protein from a Marine Bacillus subtilis Strain DM09:Potential nNovel Antibiotics against Sclerotinia Stem Rot of Rape

A Novel Antifungal Protein from a Marine Bacillus subtilis S...

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第七届国际生物防治与生物技术研讨会

作者： Zhang Jibin~1,Tian Huaping~1,Shao zongze~2,He Jin~1,Yu Ziniu~1 1.State Key Laboratory of Agricultural Microbiology,and National Engineering Research Center of Microbe Pesticides,Huazhong Agricultural University,Wuhan 430070,Hubei,China 2.Key Laboratory of Marine Biogenetic Resources,State Oceanic Administration,Xiamen,Fujian,361005,China

An antifungal protein was isolated from the culture of a marine Bacillus subtilis strain DM09 by means of ammonium sulphate precipitation,ultrafilftration and gel chromatography on a DEAE sephadex *** exhibited high inhibitory activity toward some plant pathogenic fungi,especially Sclerotinia scleroterum with an inhibitory zone diameter of 23.5 mm and IC of 0.06μg/ml. Besides that,it is relatively against heat,acid and alkali,and resistant to UV and proteolytic *** assisted laser desorption/ionization-time of flight mass spectrometry(MALDI-TOF MS) was employed to identify this protein,peptide mass fingerprints acquired from the spectra was searched in the corresponding database of matrixscience website using Mascot engine,the result indicated that this antifungal substance was a protein which had never reported by others.

关键词： marine microorganisms Bacillus subtilis Sclerotinia sclerotiorum antifungal protein

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Purification and Molecular Identification of an antifungal protein from the Hemolymph of Musca domestica(housefly)

Purification and Molecular Identification of an Antifungal P...

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格莱姆抗菌肽——抗菌肽开发与应用技术研讨会

作者： Ping Fu~1,Jianwei Wu~(1,2),Guo Guo~1 1 Department of Parasitology,Guiyang Medical College,Guiyang,Guizhou 550004,China 2 Corresponding to:Dr.Jianwei Wu,Department of Parasitology,Guiyang Medical College,9, Beijing Road,Guiyang 550004,China.

Antibacterial and antifungal peptides found in houseflies(Musca domestica)in large numbers are indispensable components of its immune defense *** this study the anterior tip of the larvae of housefly was cut off with ... 详细信息

关键词： Musca domestica antifungal protein purification molecular identification

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Fructose promotes growth and antifungal activity of Penicillium citrinum

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protein & Cell 2016年第7期7卷 527-532页

作者： Chang-wen Wu Xiaojun Wu Chao Wen Bo Peng Xuan-xian Peng Xinhua Chen Hui Li Center for Proteomics and Metabolomics State Key Laboratory of Bio-Control MOE Key Lab Aquatic Food Safety School of Life Sciences Sun Yat-sen University University City Guangzhou 510006. China Key Laboratory of Marine Biogenetic Resources Third Institute of Oceanography State Oceanic Administration Xiamen 361005 China

Dear Editor, Fungal infection involves the invasion of tissues by one or more species of fungi, and this is a serious problem in both medical settings and agriculture [Brown et al., 2012]. Thus, the development of efficient strategies for management of these infections is urgently needed [Rodriguez-Martfn et al., 2010]. Recently, a number of antifungal proteins have been reported, including AFP from Aspergillus giganteus, PAF from Penicillium chrysogenum, NAF from Penicillium nalviogense, and AnAFP from Aspergillus niger [Marx et al., 2008; Geisen 2000]. These proteins have demonstrated antifungal activity against opportunistic plant and animal pathogens, such as Fusanum sp., Botrytis sp., and Asper- gillus sp. [Meyer, 2008]. We have previously characterized an antifungal protein （PcPAF） from the culture supernatant of the fungal strain Penicillium citrinum W1, which was isolated from sediment obtained from the Southwest Indian Ocean. PcPAF is thermostable and displays antifungal activity against various pathogenic fungi, including Trichoderma viride, Fusarium oxysporum, Alternaria Iongipes, and Pae- cilomyces variotii [Wen et al., 2014]. Therefore, large-scale production of PcPAF would enable the further drug development of this compound.

关键词： Fructose antifungal Activity Fatty Acid Biosynthesis antifungal protein Differential Abundance

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A New antifungal Polypeptide From Peanut Seeds

A New Antifungal Polypeptide From Peanut Seeds

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Xiaohua Duan, Yunjie Wen, Jinhua Bin* School of Life science, South China Normal University, Guangdong Key Lab of Biotechnology for Plant Development, Guangzhou 510631, China

＜正＞Plants protect themselves from fungi using a variety of pre-formed and inducible defensive mechanisms. One of the weapons used is antifungal proteins （AFPs）. Peanut seeds are rich in proteins and can easily be

关键词： Peanut antifungal protein Aspergillus flavus

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