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A state-of-the-art review of the fabrication and characteristics of titanium and its alloys for biomedical applications

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Bio-Design and Manufacturing 2022年第2期5卷 371-395页

作者： Masoud Sarraf Erfan Rezvani Ghomi Saeid Alipour Seeram Ramakrishna Nazatul Liana Sukiman Centre of Advanced Materials Department of Mechanical EngineeringFaculty of EngineeringUniversity of Malaya50603 Kuala LumpurMalaysia Department of Materials Science and Engineering Sharif University of TechnologyAzadi Ave.P.O.Box 11365‑9466TehranIran Center for Nanotechnology and Sustainability Department of Mechanical EngineeringNational University of SingaporeSingapore 117581Singapore

Commercially pure titanium and titanium alloys have been among the most commonly used materials for biomedical applications since the 1950 s.Due to the excellent mechanical tribological properties,corrosion resistance,biocompatibility,and antibacterial properties of titanium,it is getting much attention as a biomaterial for implants.Furthermore,titanium promotes osseointegration without any additional adhesives by physically bonding with the living bone at the implant site.These properties are crucial for producing high-strength metallic alloys for biomedical applications.Titanium alloys are manufactured into the three types ofα,β,andα+β.The scientific and clinical understanding of titanium and its potential applications,especially in the biomedical field,are still in the early stages.This review aims to establish a credible platform for the current and future roles of titanium in biomedicine.We first explore the developmental history of titanium.Then,we review the recent advancement of the utility of titanium in diverse biomedical areas,its functional properties,mechanisms of biocompatibility,host tissue responses,and various relevant antimicrobial strategies.Future research will be directed toward advanced manufacturing technologies,such as powder-based additive manufacturing,electron beam melting and laser melting deposition,as well as analyzing the effects of alloying elements on the biocompatibility,corrosion resistance,and mechanical properties of titanium.Moreover,the role of titania nanotubes in regenerative medicine and nanomedicine applications,such as localized drug delivery system,immunomodulatory agents,antibacterial agents,and hemocompatibility,is investigated,and the paper concludes with the future outlook of titanium alloys as biomaterials.

关键词： Titanium and titanium alloys Biomedical application Functional properties Biocompatibility antibacterial activity Advanced manufacturing

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Biosynthesis of Flower-Shaped CuO Nanostructures and Their Photocatalytic and antibacterial Activities

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Nano-Micro Letters 2020年第2期12卷 224-234页

作者： Hafsa Siddiqui MSQureshi Fozia Zia Haque Department of Physics Sha-Shib College of Science and ManagementBhopal 462030India Optical Nanomaterial Lab Department of PhysicsMaulana Azad National Institute of TechnologyBhopal 462003India

Copper oxide nanoflowers(CuO-NFs)have been synthesized through a novel green route using Tulsi leaves-extracted eugenol(4-allyl-2-methoxyphenol)as reducing agent.Characterizations results reveal the growth of crystalline singlephase CuO-NFs with monoclinic structure.The prepared CuO-NFs can effectively degrade methylene blue with 90%efficiency.They also show strong barrier against E.coli(27±2 mm)at the concentration of 100μg mL−1,while at the concentration of 25μg mL−1 weak barrier has been found against all examined bacterial organisms.The results provide important evidence that CuO-NFs have sustainable performance in methylene blue degradation as well as bacterial organisms.

关键词： Copper oxide O.Sanctum Eugenol Biosynthesis Photocatalysis antibacterial

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A Review on Surface?Functionalized Cellulosic Nanostructures as Biocompatible antibacterial Materials

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Nano-Micro Letters 2020年第6期12卷 90-112页

作者： Mandana Tavakolian Seid Mahdi Jafari Theo GMvan de Ven Department of Chemical Engineering McGill UniversityMontrealQC H3A 0C5Canada Pulp and Paper Research Center McGill UniversityMontrealQC H3A 0C7Canada Quebec Centre for Advanced Materials(QCAM/CQMF) MontrealCanada Department of Food Materials and Process Design Engineering Gorgan University of Agricultural Science and Natural ResourcesGorganIran Department of Chemistry McGill UniversityMontrealQC H3A 0B8Canada

As the most abundant biopolymer on the earth,cellulose has recently gained significant attention in the development of antibacterial biomaterials.Biodegradability,renewability,strong mechanical properties,tunable aspect ratio,and low density offer tremendous possibilities for the use of cellulose in various fields.Owing to the high number of reactive groups(i.e.,hydroxyl groups)on the cellulose surface,it can be readily functionalized with various functional groups,such as aldehydes,carboxylic acids,and amines,leading to diverse properties.In addition,the ease of surface modification of cellulose expands the range of compounds which can be grafted onto its structure,such as proteins,polymers,metal nanoparticles,and antibiotics.There are many studies in which cellulose nano-/microfibrils and nanocrystals are used as a support for antibacterial agents.However,little is known about the relationship between cellulose chemical surface modification and its antibacterial activity or biocompatibility.In this study,we have summarized various techniques for surface modifications of cellulose nanostructures and its derivatives along with their antibacterial and biocompatibility behavior to develop non-leaching and durable antibacterial materials.Despite the high effectiveness of surface-modified cellulosic antibacterial materials,more studies on their mechanism of action,the relationship between their properties and their effectivity,and more in vivo studies are required.

关键词： Cellulose Nanocellulose Surface modification antibacterial activity Biocompatibility

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antibacterial Activity of Psidium guajava Leaf and Stem Bark Extracts on Selected Bacteria in Ugbokolo, Benue State, Nigeria

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Advances in Microbiology 2022年第10期12卷 569-578页

作者： Peter Adikwu Emmanuel Godwin Oyiwona Adejor Johnson Yuana Awua Amina Ojochide Hassan Terkaa Oluwakemisola Adenugba Ene Theresa Ebiega Biological Science Department Benue State University Makurdi Nigeria Department of Science Laboratory Technology Benue State Polytechnic Ugbokolo Nigeria Benue State Ministry of Energy Science and Technology Makurdi Nigeria Department of Microbiology Federal University of Agriculture Makurdi Nigeria

Aim: In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. The study was aimed at determining the phytochemical constituents and in vitro antibacterial activity of methanol and aqueous extracts of Psidium guajava leaves and stem bark on Escherichia coli, Salmonella typhi, Staphylococcus aureus and Proteus sp. in Ugbokolo, Nigeria. Materials and Methods: The phytochemical screening of the plant materials for various bioactive components was conducted between July and December, 2019 using standard laboratory techniques. The extracts were purified using column chromatography. The identity of the test isolates were confirmed using morphological characteristics, gram stain, motility and appropriate biochemical tests such as indole, catalase, coagulase, triple sugar iron agar. The susceptibility of the isolates to each bioactive component was determined using the agar well diffusion method. The broth dilution method was employed for the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts. Results: The result of the study showed the presence of phenol, tannins, flavonoids and saponins as bioactive compounds. The antibacterial susceptibility of the isolates to aqueous and methanol extracts of leaf and stem bark of Psidium guajava varied significantly (P Staphylococcus aureus was the most susceptible isolate at 200 mg/ml concentration with average zone of inhibition of 13.05 mm for leaf extract and 15.34 mm for stem bark extract. Proteus sp. is the least susceptible with average zone of inhibition of 8.88 mm for the leaf extract and 12.36 mm for the stem bark extract respectively. Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of aqueous and methanol extract of P. guajava leaf and stem bark showed that dilutions of various concentrations of aqueous and methanol extracts can inhibit and/or kill the isolates. Lower MIC (3.125 mg/ml)

关键词： antibacterial Psidium guajava Leaf Extract Stem Bark Extract

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Microwave mediated synthesis and characterization of CeO_2-GO hybrid composite for removal of chromium ions and its antibacterial efficiency

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Journal of Environmental Sciences 2019年第2期31卷 65-79页

作者： Lellala Kashinath Keerthiraj Namratha Kullaiah Byrappa Department of studies in Earth Science MansagangothriUniversity of MysoreMysore 570006India Centre for Materials Science and TechnologyVijana BhavanManasagangothriUniversity of Mysore

A facile fabrication and processing of cerium oxide-graphene oxide(CeO_2-GO) hybrid nanocomposites without the use of any surfactant or any organic solvents using chemical method and treatment with microwave irradiation technique are reported. In-situ hexagonal nano cerium oxide particles embedded on the layered surface of GO sheets were investigated for the photodegradation of dyes, removal of chromium Cr(VI) ions and against antibacterial studies. The results imply that hybrid nanocomposites shows enhanced 5-folds of photocatalytic activities in UV(ultraviolet) light irradiation and exhibited rapid efficiency in the elimination of chromium ion better than the pure GO and CeO_2, which are inhibited by competent photosensitive electron inoculation and controlling the electron–hole recombination. The synergetic effect of CeO_2-GO composites played a vital role in showing better results against model bacterium than GO and CeO_2 are due to higher physical interaction endorsed to the stress of membranes acute by piercing edges,large surface area, and higher adsorptive conditions of graphene oxide sheets tailored with ceria particles. The amount of charge transferred at the interface increases with the concentration of O atoms, demonstrating the interaction between CeO_2 and GO is much stronger than CeO_2 and GO are due to the decrease of the average equilibrium distance between the interfaces. The CeO_2-GO interface staggered band alignments existing between the CeO_2 surfaces and GO which shows an excellent synergism. The structure and morphology of composites were tested by X-ray diffraction(XRD), Fourier transform infrared(FTIR), Raman, X-ray photoelectron spectroscopy(XPS), and high-resolution transmission electron microscope(HR-TEM).

关键词： Ceria Graphene oxide Chromium Photodegradation antibacterial

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antibacterial potency of methanol extracts of lower plants

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第3期8卷 189-191页

作者： OJO O.O. AJAYI A.O. ANIBIJUWON I.I. Department of Microbiology Faculty of Science University of Ado-Ekiti Ado-Ekiti Ekiti State 234030 Nigeria

antibacterial potency of methanol extracts of three green lower plants, Pneumatopteris afra, Platycerium bifurcatum and Nephrolepsis bisserata was determined using agar dilution method on clinical strains of Escherichia coli, Staphylococcus aureus, Klebsiella spp. and Salmomelia typhi. antibacterial activities were observed at concentrations of 12.5, 25.0, 50.0 and 100.0 μg/ml. Their minimum inhibitory concentrations ranged from 12.5~100 μg/ml. Extracts of P. afra and P. bifurcatum were most active. antibacterial activities observed with N. bisserata were less pronounced with no detectable activity at extract con-centrations of 12.5 and 25.0 μg/ml. E. coli, together with S. aureus appeared to be the most susceptible of the test bacteria while Klebsiella spp. was least sensitive. The significance of our findings is discussed.

关键词： Lower plants Extracts antibacterial activity MIC (minimum inhibitory concentration)

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antibacterial Behavior of Pyridinecarboxylatesilver（Ⅰ） Complexes

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Chinese Journal of Chemistry 2012年第7期30卷 1631-1635页

作者： Abarca, Romina Gomez, Grace Velasquez, Carla Paez, Maritza A. Gulppi, Miguel Arrieta, Abel Azocar, Manuell Laboratory of Bioinorganic Chemistry Faculty of Chemistry and Biology University of Santiago of Chile Av. Bernardo O'Higgins 3363 Santiago Chile

FT-1R spectroscopy of carboxylic groups and viability tests were useful to understand the antibacterial proper- ties of six highly efficient silver（I） pyridinecarboxylate （nicotinic, picolinic and isonicotinic acids） and bipiridinecarboxylate （pyridine-2,3-dicarboxylic, pyridine-2,4-dicarboxylic and pyridine-2,5-dicarboxylic acids） complexes with Ag--O and Ag--N bonds against E. coli （ATCC 25922） and Streptococcus＇ agalactiae （ISP 329-09）. The resuits show a tendency between the nature of Ag--X （X=oxygen and nitrogen） bonds and the rate or efficiency of antibacterial behavior.

关键词： silver complexes antibacterial Ag--O and Ag--N bonds FTIR

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Synergistic antibacterial photocatalytic and photothermal properties over bowl-shaped TiO_(2)nanostructures on Ti-19Zr-10Nb-1Fe alloy

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Regenerative Biomaterials 2022年第1期9卷 271-279页

作者： Yan Wu Zichao Deng Xueying Wang Aihua Chen Yan Li School of Materials Science and Engineering Beihang UniversityBeijing 100191China Beihang Hangzhou Innovation Institute Yuhang Beihang UniversityHangzhou 310023China Biomaterials Laboratory of the Medical Device Inspection Institute National Institutes for Food and Drug ControlBeijing 102629China Beijing Advanced Innovation Centre for Biomedical Engineering Beihang UniversityBeijing 100191China

As implant substitutes are increasingly applied to the clinic,the infection caused by implants has become one of the most common complications,and the modification of the antibacterial function of the implant can reduce such complications.In this work,a well-defined bowl-shaped nanostructure coating with photocatalytic and photothermal synergistic antibacterial properties was prepared on Ti-19Zr-10Nb-1Fe(TZNF)alloy.The coating is obtained by spin-coating and sintering TiO_(2)precursors templated from self-assembled microspheres of polystyrenepoly(4-vinylpyridine)(PS-P4VP)amphiphilic block polymer on TZNF alloy.PS-P4VP provides the bowl-shaped TiO_(2)nanostructures doped with C,N elements,reducing the band-gap of TiO_(2),which can absorb near-infrared(NIR)light to release reactive oxygen species and produce photothermal conversion.The bowl structure is expected to enhance the utilization of light via the reflection in the confined space.The bowl-shaped surface has 100%antibacterial rates after 30 min of NIR light irradiation.In addition to antibacterial properties,the bowl-shaped surface has better hydrophilicity and protein adsorption capacity.The amount of protein adsorbed on TZNF with the bowl-shaped structures was six times that of TZNF.Hence,the bowl-shaped nanostructure can promote the proliferation and adhesion of osteoblasts,the cell proliferation rate was increased by 10–30%.

关键词： bowl-shape TiO_(2) Ti-19Zr-10Nb-1Fe alloy antibacterial photocatalytic photothermal

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antibacterial activities of selected medicinal plants in traditional treatment of human wounds in Ethiopia

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Asian Pacific Journal of Tropical Biomedicine 2011年第5期1卷 370-375页

作者： Biruhalem Taye Mirutse Giday Abebe Animut Jemal Seid Aklilu Lemma Institute of Pathobiology P.O.BoxAddis Ababa University ALERT Hospital Addis AbabaEthiopia

Objective:To evaluate the activity of selected Ethiopian medicinal plants traditionally used for wound treatment against wound-causing bacteria.Methods:Samples of medicinal plants(Achyranthes aspera,Brucea antidysenteriea,Datura stramonium,Croton macrostachyus,Acokanthera xchimperi.,Phytolacca dodecandra,Milhttia ferruginea,and Solanum incanum)were extracted using absolute methanol and water and tested for their antimicrobial activities against clinical isolates and standard strains of wound-causing bacteria using agar well diffusion and micro titer plate methods.Results:Most of the plant extracts had antibacterial activities,among which Acokanthera schimperi and Brucea antidysenteriea inhibited growth of 100%and 35%of the test organisms,respectively.Methanolic extracts had higher activities compared with their corresponding aqueous extracts.The most susceptible organism to the extracts was Streptococcus pyogens while the most resistant were Escherichia coli and Proteus vulgaris.Conclusions:This finding justifies the use of the plants in wound healing and their potential activity against woundcausing bacteria.Their toxicity level and antimicrobial activity with different extraction solvents should further be studied to use them as sources and templates for the synthesis of drugs to control wound and other disease-causing bacteria.

关键词： antibacterial activity Medicinal plants Human wounds Ethiopia Agar well diffusion Micro titer plate Plant extracts

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antibacterial activity of some actinomycetes from Tamil Nadu,India

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Asian Pacific Journal of Tropical Biomedicine 2012年第12期2卷 936-943页

作者： Pachaiyappan Saravana Kumar John Poonga Preetam Raj Veeramuthu Duraipandiyan Savarimuthu Ignacimuthu Division of Microbiology Entomology Research InstituteLoyola College Department of Botany and Microbiology College of ScienceKing Saud University

Objective:To isolate novel actinomycetes and to evaluate their antibacterial activity.Methods:Three soil samples were collected from Vengodu(village)in Kanchipuram district,Tamil Nadu,India.Actinomycetes were isolated using serial dilution and plating method on actinomycetes isolation agar.Results:Totally 35 isolates were obtained on the basis of colony characteristics on actinomycetes isolation agar.All the isolates were screened for antibacterial activity by cross streak method.Medium and optimization of day were done for the potent strains using Nathan's agar well diffusion method.Isolation of bioactive compounds from significant active isolates was done by using different media.The most active isolate VAS 10 was identified as Actinobacterium Loyola PBT VAS 10(accession No.JF501398)using 16s rRNA sequence method.The hexane,ethyl acetate,dichloromethane and butanol extracts of VAS 10 were tested against bacteria.The maximum antibacterial activity was observed in dichloromethane and ethyl acetate;maximum zones of inhibition were observed against Enterococcus durans.The rRNA secondary structure and the restriction sites of Actinobacterium Loyola VAS 10 were predicted using Genebee and NEBCutter online tools respectively.Conclusions:The present study showed that among the isolated actinomycetes,Actinobacterium Loyola PBT VAS 10(accession No.JF501398)showed good antibacterial activity against the tested bacteria.

关键词： VAS10 Actinobacterium antibacterial activity Solvent extract

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