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第十五次全国动物遗传育种学术讨论会

作者： Wilson PW Bain MM Kalina J Dunn Ian Roslin Institute and Royal(Dick) School of Veterinary Studies University of EdinburghRoslinMidlothian EH25 9PS Scotland. University of Glasgow Faculty of Veterinary MedicineGlasgowG611QHScotland.

＜正＞Introduction Gallin was first noted using a proteomic approach to the analysis of chicken egg white（Mann,2007）.It was named gallin because of its homology to meleagrin,a protein previously discovered as a contaminant in a turkey （Meleagris gallopavo） ovomucin preparation（Odani et al.,1989） and to cygnin,discovered in the preparation of

关键词： Gallin antibacterial peptide chicken

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Purification of a Novel antibacterial Short peptide in Earthworm Eisenia foetida

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 297-302页

作者： Yan-Qin LIU1,2, Zhen-Jun SUN1*, Chong WANG1, Shi-Jie LI2, and Yu-Zhi LIU2 College of Resource and Environment Science, China Agricultural University, Beijing 100094, China 2College of Animal Science, Hebei Agricultural University, Baoding 071001, China College of Resource and Environment Science China Agricultural UniversityBeijing 100094 China College of Animal Science Hebei Agricultural UniversityBaoding 071001 China

A novel antimicrobial short peptide was purified from earthworm (Eisenia foetida) by a five-step protocol including ammonium sulfate precipitation, ultrafiltration, DE-52 ion exchange chromatography, Sephadex G-10 column chromatography, and C-18 reversed-phase HPLC techniques. The purified peptide was applied to the MALDI-TOF MS to determine the molecular mass and was also subjected to TOF MS-MS analysis to determine the amino acid sequence. As a result, a novel antibacterial peptide, named OEP3121, was obtained, with the molecular mass of 510.8 Da and the sequence being “ACSAG”.

关键词： antibacterial peptide earthworm purification

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Effect of Dietary antibacterial peptide and Zinc-methionine on Performance and Serum Biochemical Parameters in the Weaned Piglets*

Effect of Dietary Antibacterial Peptide and Zinc-methionine ...

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第五届中国畜牧科技论坛

作者： J.H.Wang C.C.Wu J.Feng Laboratory of Medicinal Chemistry Department of Bioengineering CollegeChongqing University

The objective of this study was to evaluate the effect of dietary supplementation of antibacterial peptide and zinc-methionine on performance and some serum biochemical parameters of weaned piglets. Rongchang male piglets(28 days of age,initial weight 8.4±0.65 Kg) were *** piglets were randomly allotted to four diets including the control group,the antibacterial peptide(AP) group containing 10 mg AP/kg diet, the zinc-methionine(Zn-Met) group with 1200 Zn-Met/kg diets(equal to 200mg Zn/kg diets),the Zn/AP group containing 10mg AP and 1200mg Zn-Met/kg diet,respectively,in a 4-week feeding *** of these groups consisted of six replications with 8 pigs per *** ADG and ADFI all improved for the AP group(P0.05,P<0.05),Zn-Met group(P0.05,P<0.05) and Zn/AP group(P<0.01,P<0.05),as compared with the *** joint use of Zn-Met and AP was synergetic(P<0.05) on improving F/G(feed intake/gain ratio). The IgG and SOD were increased(P<0.05) and TC decreased(P<0.05) for the AP group and the Zn-Met group, the HDL increased(P<0.05) for the Zn-Met group,as compared with the *** addition,the changes of serum TP and serum HDL for the control showed regularity during the *** results indicated that AP and ZnMet were effective in improving growth performance and some serum parameters levels of weaned piglets.

关键词： antibacterial peptide Zinc Methionine Weaned piglet Performance Serum parameters

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Induction,selection and antibacterial activity of the antibacterial peptides from lepidopteran insect cultured cell lines

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生物学前沿:英文版 2008年第2期3卷 203-206页

作者： Rong PENG Zhong YANG Kaiyu LIU Hanchao YAO Hong YANG Yanfang CUI Huazhu HONG College of Life Science Central China Normal UniversityWuhan 430079China College of Bioengineering East China University of Science and TechnologyShanghai 200237China College of Chemistry Central China Normal UniversityWuhan 430079China

We induced 3 cell lines that were in vitro cultured from Lepidoptera with heat inactivated Escherichia coliDH_(5α) to stimulate the antibacterial peptide followed by antibacterial activity assay,induction dynamic research and Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis(Tricine SDS-PAGE)*** antibacterial activity of the induced BTI-Tn-5B1 cell line was the highest,and the antibacterial activity increased gradually to the highest level in 16 hours after stimulation.A new antibacterial peptide with a molecular weight of about 8000 Da was preferentially induced in Trichoplusia ni BTI-Tn-5B1 cells in 16 hours after *** activity assays indicated that it had inhibition against Staphylococcus aureus,Escherichia coli K_(12)D_(31) and Salmonella *** has especially strong inhibition against Gram-negative bacteria such as Escherichia coli K_(12)D_(31) and Salmonella derby.

关键词： antibacterial peptide BTI-Tn-5B1 cell line Tricine SDS-PAGE antibacterial activity

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antibacterial function and mechanism of peptide β-casein 65 from breast milk

Antibacterial function and mechanism of peptide β-casein 65...

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第十三届江浙沪儿科学术会议暨2016年浙江省医学会儿科学学术年会

作者： Jun Zhang Lianghui You Yanrong Fu Xiao Liu Xing Wang Yun Li Xianwei Cui Chenbo Ji Xirong Guo Department of Pediatrics Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University Maternal and Child Health Institute Nanjing Maternity and Child Health Care Hospital

Breast milk which has an effective function on preventing infection is the ideal food for newborns.β-casein65 is a novel endogenous peptides found in preterm milk by Liquid chromatography-mass spectrometry/mass spectrometry(LC-MS/MS) *** experiments confirm that β-casein65 has strong antibacterial activity on common newborn infection bacteria such as *** and *** by agarose filter paper diffusion method and microdilution *** use Live/Dead Baclight Bacterial Viability Kit test *** and *** treated with β-*** observe the cell membrane and inner structure by scanning electron microscope and transmission electron *** found that β-casein 65 has cell killing and injury effect by damage cell *** membrane exhibits wrinkles,holes,or even *** interaction between β-casein65 and the genomic DNA or RNA was examined by agarose gel *** was no significant difference when *** and *** treated with β-*** study confirmed the antibacterial function and possible mechanism of β-casein *** study will be carried out on animal model.β-casein65 will have a huge potential clinical value for preventing infection.

关键词： antibacterial peptide β-casein 65 peptide breast milk

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Molecular structure,chemical synthesis,and antibacterial activity of ABP-dHC-cecropin A from drury（Hyphantria cunea）

Molecular structure,chemical synthesis,and antibacterial act...

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江苏省遗传学会2014年学术研讨会

作者： Jiaxin Zhang Ali Movahedi Xiaoli Wang Xiaolong Wu Tongming Yin Qiang Zhuge Key Laboratory of Forest Genetics and Biotechnology Ministry of EducationNanjing Forestry University

The increasing resistance of bacteria and fungi to currently available antibiotics is a major concern world-wide,leading to enormous efforts to develop new antibiotics with new modes of *** this paper,cDNA encoding cecropin A was amplified from drury（Hyphantria cunea）（dHC） pupa fatbody total RNA using *** full-length dHC-cecropin A cDNA encoded a protein of 63 amino acids with a predicted 26-amino acid signal peptide and a 37-amino acid functional *** synthesized the antibacterial peptide（ABP） from the 37-amino acid functional domain（ABP-dHC-cecropin A）,and amidated it via the ***-of-flight mass spectrometry showed its molecular weight to be *** ABP-dHC-cecropin A was assessed in terms of its protein structure using bioinformatics and CD *** protein’s secondary structure was predicted to *** an antibacterial activity analysis,the ABP-dHC-cecropin A exhibited strong antibacterial activity against *** K12 D31 and Agrobacterium EHA105.

关键词： Hyphantria cunea antibacterial peptide ABP-dHC-cecropin A Molecular structure antibacterial activity

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Application of antimicrobial peptides in production life

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Microenvironment & Microecology Research 2021年第4期3卷 6-9页

作者： Yi-Bo Yang Jia-Hao Li Yu-Na Zhang Li-Jun Xu Rui Zhang Chun-Ming Dong College of Marine and Environmental Sciences Tianjin University of Science and TechnologyTianjin 300457China School of Light Industry Science and Engineering Tianjin University of Science and TechnologyTianjin 300457China.

antibacterial peptide is a small molecular weight,a substance existing in the biological body,plays a very important role in congenital immune ***,in various antibiotics,the problem of drug resistance has caused people to live an indelible *** the antibacterial peptide has spectral antibacterial properties,it has the characteristics of participating in human natural immunity and no residual,and is now one of the research and development *** peptide is a recognized substance that can replace antibiotics,and people have never stopped,and new types of antimicrobial peptides have also emerged in the Volkswagen *** paper is based on the use of antimicrosis peptides in daily life,briefly describes the effects of antimicrobial peptides in medical,animal husbandry and industrial.

关键词： antibacterial peptide Role Research status

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