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Sheng Jiang San, traditional multi-herb formulation, exerts anti-influenza effects in vitro and in vivo via neuraminidaseinhibition and immune regulation

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中国药理学与毒理学杂志 2019年第9期33卷 738-739页

作者： ZHANG Tian-bo XIAO Meng-jie Chun-Kwok WONG Ka-Pun CHRIS MOK ZHAO Xin TI Hui-hui Pang-Chui SHAW State Key Laboratory of Respiratory Disease Guangdong Provincial Key Laboratory of Molecular Target&Clinical PharmacologySchool of Pharmaceutical Sciences and The Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhouChina The School of Life Sciences The Chinese University of Hong KongHong KongChina School of Public Health Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina

OBJECTIVE Sheng Jiang San(SJS),a multi-herb formulation,is used in treating high fever,thirsty and anxiety in ancient China and it is sometimes used to treat seasonal influenza in ***,there is no evidencebased investigation and mechanism research to support SJS′s anti-influenza *** study aims to investigate the anti-influenza effect of SJS and its possible *** In this study,we examined the inhibitory effect of SJS against different influenza viruses on Madin-Darby canine kidney *** virus infected BALB/c mice were employed as in vivo model to evaluate the *** challenged with A/PR/8/34(H1N1)were orally administrated SJS 1 g·kg^-1 daily for seven days and monitored for 14 *** survival rate,body mass changes,lung index,lung viral load,histopathologic changes and immune-regulation of the mice were *** underlying anti-influenza virus mechanisms were studied by a series of biological assays in vitro to determine if hemagglutinin,ribonucleoprotein complex or nerauminidase were targets of *** SJS exerted a broad spectrum of inhibitory effects on multiple influenza strains in a dose-dependent *** IC50 of SJS against A/WSN/33(H1N1)was lower than 35 mg·L^-*** also protected 50%of mice from influenza virus PR8 *** lung index and the lung viral load of SJS treated mice were signifi⁃cantly decrease compared with untreated *** 2 g·L^-1 inhibited 80%of neuraminidase enzymatic *** also up-regulated TNF-αand IFN-αand down-regulated IL-2 of influenza virus induced *** SJS is a useful formulation for treating influenza virus infection.

关键词： Sheng Jiang San anti-influenza activity neuraminidase inhibition immuno-regulation

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Additive Effects of Water-Soluble Propolis(Greit 120)and Human Interferon Alfa(HuIFN-αN3)against influenza Viruses in Vitro

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Journal of Agricultural Science and Technology(A) 2018年第1期8卷 36-47页

作者： Bratko Filipic Lidija Gradisnik Adriana Pereyra Rok Kopino Klemen Rihar Eva Ruzic-Sabljic Snezana Kramar Damir Dermie Eugen Soos Nicola Volpi Alfredo Fachini and Hrvoje Mazija Croatian Institute of Experimental and Translation Oncology Koledinecka 3Zagreb 10040Croatia Institute of Biomedical Sciences Medical Faculty of MariborTaborska 8Maribor 2000Slovenia Medex D.o.o. Linhartova 49bLjubljana 1000Slovenia Chengdujska 4 Ljubljana 1000Slovenia Institute of Microbiology and Immunology Medical FacultyZaloska 4Ljubljana 1105Slovenia Division of Molecular Biology Ruaer Boskovic InstituteBijenicka 54Zagreb 10000Croatia Trg Sv.Ivana 5 Klostar Ivanic 10312Croatia Department of Life Sciences University of Modena&Reggio EmiliaModena 41125Italy BNatural Via Gran Sasso 33Corbetta-MI 20011Italy

influenza virus affects the respiratory tract in humans causing a range of distinct manifestations including fever, nasal secretions, cough, headaches, muscle pain and pneumonia, which could become violent and severe. It was found that influenza A viruses remain resistant to amantadine and rimantadin with high level of oseltamvir resistance. Therefore, there is a need for constant improvement of drugs active against resistant influenza viruses. Propolis has anti-influenza activity both in vitro and in vivo. Human leukocyte interferon （HuIFN-αN3） is a multi-subtype protein that displays an antiviral activity against influenza virus. In this study we elucidated the anti-influenza activity of the mixes of water-soluble propolis （WSP）（Greit 120） and HuIFN-αN3 at different ratios. Greit 120 polyphenols, total phenol acids and bioflavonoid were characterized by HPLC-UV-ESI-MS504971 and HuIFN-αN3 by reverse-phase high-performance liquid chromatography （RP-HPLC）. influenza A and B viruses were separately added to the LLC-MK2 cells treated with WSP （Greit 120） and HuIFN-αN3 alone or in proportions 1：1, 1：2 and 2：1. Plates were incubated and cytopathic effect was determined. The best results （ID50） were obtained with the mix of 10% WSP and HuIFN-αN3 in proportion 1：2, showing ID50 at 12 ± 0.2 μg/mL and 19 ± 0.6 μg/mL for influenza A and B viruses, respectively. When comparing anti-influenza activity of WSP （Greit 120）/HuIFN-αN3 with that of ribavirin, it was found that 1：2 was the optimal ratio for WSP （Greit 120）/HuIFN-αN3 （0.5 and 0.6 for influenza A and B, respectively）. This new formulation of WSP （Greit 120） and HuIFN-αN3, showing better anti-influenza activity, will definitely improve its application in flu infections.

关键词： Water-soluble propolis Greit 120 HuIFN-αN3 anti-influenza activity ribavirin ID_(50)

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