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Single intratesticular injection of blood-serum-derived exosomes can potentially alleviate testopathy following testicular torsion

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Animal Models and Experimental Medicine 2022年第4期5卷 362-368页

作者： Mona Keivan Fatemeh Mansouri Torghabeh Samira Davoodi Shima Moradi Maryamneghari Reza Dadfar Member of Research Committee Medical SchoolKermanshah University of Medical SciencesKermanshahIran Department of Physiology Sciences Medical SchoolMashhad University of Medical SciencesMashhadIran Department of Anatomical Sciences Medical SchoolKermanshah University of Medical SciencesKermanshahIran Department of Anatomical Sciences Faculty of MedicineAJA University of Medical SciencesTehranIran

Background:Testicular torsion(TT)is an acute inflammatory process leading to male infertility.Today,anti-i nflammatory effects of exosomes derived from blood serum are used in various laboratory procedures.in the present study,the anti-i nflammatory effects of blood-serum-derived exosomes in treatment of acute inflammation following TT in mice were evaluated.Materials and Methods:Eighteen male mice were grouped as healthy control,TT,and TT+exosome.TT was induced surgically,and exosomes were extracted from blood serum and administrated by a single intratesticular injection(10 iU).Malondialdehyde(MDA)and Griess assays were used to evaluate the level of oxidative stress.Sperm indices,testosterone(Tes),and apoptotic gene expression(p-53,Bcl2,and Caspase-3)were also assessed.H&E and immunohistochemistry(iHC)stainings were used for histopathological investigations.Data analysis was applied by SPSS(v.19)software.Results:Oxidative stress and apoptotic genes expression were increased significantly(pin TT group compared with control.Sperm parameters and Tes were significantly increased,and expression of apoptotic genes was significantly reduced in TT+exosome group(pion:Since the blood-serum-derived exosomes have anti-i nflammatory features,the intratesticular application of blood-serum-derived exosomes can be used clinically in acute phase of orchitis following TT to inhibit testicular inflammation.

关键词： anti-i nflammation exosome parameter sperm testicular torsion

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Ephedrine hydrochloride inhibits PGN-induced inflammatory responses by promoting iL-10 production and decreasing proinflammatory cytokine secretion via the Pi3K/Akt/GSK3β pathway

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Cellular & Molecular immunology 2013年第4期10卷 330-337页

作者： Yuejuan Zheng Yang Yang Yuhu Li Limin Xu Yi Wang Ziyi Guo Haiyan Song Muyi Yang Beier Luo Aoxiang Zheng Ping Li Yan Zhang Guang Ji Yizhi Yu Department of immunology and Microbiology Shanghai University of Traditional Chinese Medicine Shanghai 201203 China National Key Laboratory of Medical immunology & institute of immunology Second Military Medical University Shanghai 200433 China institute of Digestive Disease Longhua Hospital Shanghai University of Traditional Chinese Medicine Shanghai 200032 China

Approaches for controlling inflammatory responses and reducing the mortality rate of septic patients remain clinically ineffective; new drugs need to be identified that can induce anti-inflammatory responses. Ephedrine hydrochloride （EH） is a compound that is widely used in cardiovascular diseases, especially to treat hypotension caused by either anesthesia or overdose of antihypertensive drugs. in this study, we reported that EH also plays an important role in the control of the inflammatory response. EH increased iL-iO and decreased proinflammatory cytokine （iL-6, tumor-necrosis factor （TNF）-a, iL-12 and iL-11~） expression in primary peritoneal macrophages and Raw264.7 cells treated with peptidoglycan （PGN）, a Gram-positive cell wall component. The anti-inflammatory role of EH was also demonstrated in an experimental mouse model of peritonitis induced by intraperitoneal PGN injection. The phosphatidylinositol 3-kinase （Pi3K）/Akt pathway was found to be responsible for the EH-mediated increase in iL-iO production and decrease in iL-6 expression. Therefore, our results illustrated that EH can help maintain immune equilibrium and diminish host damage by balancing the production of pro- and anti-inflammatory cytokines after PGN challenge. EH may be a new potential anti-inflammatory drug that can be useful for treating severe invasive Gram-positive bacterial infection.

关键词： anti-i nflammation EH ephedrine hydroch ioride i L- 10 peptidoglycan PG N phosphatidylinositol 3-ki nase Pi3K

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