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Acta Pharmaceutica Sinica B 2015年第3期5卷 201-209页

作者： Zhi Jiang Huiqiang Wang Yanping Li Zonggen Peng Yuhuan Li Zhuorong Li Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College

A series of novel N-phenylbenzamide and N-phenylacetophenone compounds were synthesized and evaluated for their antiviral activity against hcv and EV71(strain SZ-98).The biological results showed that three compounds(23,25 and 41) exhibited considerable anti-hcv activity(IC50? 0.57–7.12 μmol/L) and several compounds(23,28,29,30,31 and 42) displayed potent activity against EV71 with the IC50 values lower than 5.00 μmol/*** potency of compound 23(IC50? 0.57 μmol/L) was superior to that of reported compounds IMB-1f(IC50? 1.90 μmol/L) and IMB-1g(IC50? 1.00 μmol/L) as anti-hcv agents,and compound29 possessed the highest anti-EV71 activity,comparable to the comparator drug *** efficacy in vivo and antiviral mechanism of these compounds warrant further investigations.

关键词： Synthesis N-phenylbenzamide N-phenylacetophenone anti-hcv anti-EV71 Agents

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Synthesis and biological evaluation of sophocarpinic acid derivatives as anti-hcv agents

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Acta Pharmaceutica Sinica B 2014年第4期4卷 307-312页

作者： Yinghong Li Zonggen Peng Limei Gao Danqing Song Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

Chronic hepatitis C virus(hcv)infection has become a major public health burden ***-two sophocarpinic acid or matrine derivatives were synthesized and their anti-hcv activities were evaluated in *** structure-activity analysis revealed that(i)sophocarpinic acids with a D-seco 3-ring structure scaffold were more favorable than matrines with a 4-ring scaffold;(ii)the introduction of an electron-withdrawing group on the phenyl ring in 12-N-benzenesulfonylΔβγsophocarpinic acids was beneficial for the antiviral activity against *** them,compounds 9h and 9j exhibited the most potent inhibitory activities on hcv replication with selectivity indies of 70.3 and 30.9,***,both were selected as antiviral candidates for further investigation.

关键词： Sophocarpinic acid Matrine anti-hcv antiviral activity Structure-activity relationship

Prevalence of anti-hcv antibodies Among Thalassemia Patients in Mosul City, Iraq

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Journal of Life Sciences 2012年第5期6卷 489-491页

作者： Mohammed D. Khalid Basima A. Abdullah College of Science University of Mosul Mosul Iraq

The study included 200 thalassemia patients, 120 （60%） males and 80 （40%） females, at age 2-30 years the mean age 16 years and 100 persons from blood donors at age 20-50 years, the mean age 35 years, as control group. The authors used ELISA hcv 3.0 to detect anti-hcv in serum of thalassemia patients and blood donors. The results show 17% positive, 76% negative and 7% equivocal in serum ofthalassemia patients, but show 1% positive, 98% negative, 1% equivocal in serum of blood donors. The results show significant difference in P ≤ 0.05 between prevalence of anti-hcv antibodies among thalassemia patients and blood donors, the confirmatory tests by recombinant immunoblot assay version 3.0 （RIBA） show 17.5% positive, 79% negative and 3.5% equivocal in serum of thalassemia patients but show 2% positive, 98% negative in serum of blood donors. The thalassemia patients in the present study region had high seroprevalence of anti-hcv.

关键词： Thalassemia patients blood donors anti-hcv serum ELISA hcv 3.0 recombinant immunoblot assay version 3.0.

HBsAg及anti-hcv阳性与胆汁反流性胃炎的关系探讨

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中外医疗 2015年第34期34卷 73-75页

作者：滕海波单小惠郑耀丽罗林张千里赤峰宝山医院消化科内蒙古赤峰024076

目的探讨HBs Ag及anti-hcv阳性与胆汁反流性胃炎的相关关系。方法查阅2006年6月—2015年5月于该院胃镜检查、并详实保存检查资料的20 316例患者的镜检结果,统计并对比HBs Ag和anti-hcv阳性与阴性,以及HBs Ag阳性和anti-hcv阳性病人之间... 详细信息

目的探讨HBs Ag及anti-hcv阳性与胆汁反流性胃炎的相关关系。方法查阅2006年6月—2015年5月于该院胃镜检查、并详实保存检查资料的20 316例患者的镜检结果,统计并对比HBs Ag和anti-hcv阳性与阴性,以及HBs Ag阳性和anti-hcv阳性病人之间胆汁反流性胃炎发生率的差异,进行统计学分析。结果 1HBs Ag与antihcv均阴性17 041例,胆汁反流性胃炎1 292例,发生率为7.58%;HBs Ag阳性与anti-hcv阳性合计3 275例,发生胆汁反流766例,发生率为23.39%,两组差异有统计学意义(P﹤0.05)。2HBs Ag阳性649例,胆汁反流性胃炎206例,发生率31.74%;anti-hcv阳性2 626例,发生胆汁反流性胃炎560例,发生率21.33%,两组差异有统计学意义(P﹤0.01)。结论 HBs Ag阳性及anti-hcv阳性较阴性患者容易发生胆汁反流性胃炎;HBs Ag阳性患者胆汁反流性胃炎发生率较anti-hcv阳性为高。

关键词： HBsAg anti-hcv 胆汁反流性胃炎

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静脉注射经anti-hcv筛检的免疫球蛋白后发生急性丙型肝炎

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国际病毒学杂志 1997年第1期4卷 31-32页

作者：高斌

静脉注射经ａｎｔｉ－ＨＣＶ筛检的免疫球蛋白后发生急性丙型肝炎［英］／ＣｈｒｉｓｔｏｐｈｅｒＪ．Ｈｅａｌｙ…／／ＧｒａｓｔｒｏｅｎｔｅｒｏｌｏｇｙＡｐｒｉｌ．－１９９６，１１０（４）．－１１２０～１１２６静脉用免疫球蛋... 详细信息

静脉注射经ａｎｔｉ－ＨＣＶ筛检的免疫球蛋白后发生急性丙型肝炎［英］／ＣｈｒｉｓｔｏｐｈｅｒＪ．Ｈｅａｌｙ…／／ＧｒａｓｔｒｏｅｎｔｅｒｏｌｏｇｙＡｐｒｉｌ．－１９９６，１１０（４）．－１１２０～１１２６静脉用免疫球蛋白（ＩＶＩｇ）是治疗免疫缺陷疾病的...

关键词：丙型肝炎病毒性肝炎免疫球蛋白 anti-hcv 筛检静脉注射

化学发光法检测anti-hcv在临床中的应用价值分析

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中文科技期刊数据库（引文版）医药卫生 2023年第5期 88-90页

作者：赵红梅兴安两江医院广西桂林541300

研究分析化学发光法对人抗丙型肝炎病毒抗体(Human Hepatitis C virus antibodies,anti-hcv)检测的临床应用价值。方法:随机选取我院2021年1月至2022年12月的30例丙型肝炎患者,将其视为本研究观察对象,对其采取时间分辨荧光免疫法、化... 详细信息

研究分析化学发光法对人抗丙型肝炎病毒抗体(Human Hepatitis C virus antibodies,anti-hcv)检测的临床应用价值。方法:随机选取我院2021年1月至2022年12月的30例丙型肝炎患者,将其视为本研究观察对象,对其采取时间分辨荧光免疫法、化学发光法对其anti-hcv进行检查,研究分析其临床应用价值。结果:化学发光法临床anti-hcv检出率高于时间分辨荧光免疫法,且检测时间低于时间分辨荧光免疫法,具备统计学差异(Panti-hcv中使用化学发光法取得的临床应用价值较高,与时间分辨荧光免疫法相比,化学发光法能够提高临床检测的准确率,缩短检测时间,能够为临床诊疗方案的制定给予良好的参考依据,值得推荐。

关键词：化学发光法时间分辨荧光免疫法 anti-hcv 临床应用价值

化学发光微粒子免疫分析法检测anti-hcv结果的假阳性分析

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现代医学与健康研究电子杂志 2018年第20期2卷 20-21页

作者：陈影姚玮王庆保余金芸安徽中医药大学第一附属医院检验中心安徽合肥230031

目的分析化学发光微粒子免疫分析法(CMIA)检测anti-hcv阳性结果中假阳性的情况。方法选取本实验室测定anti-hcv结果为阳性的标本67例,用RT-PCR方法检测血清中hcv-RNA的载量,判断标准:hcv-RNA(+)为真阳,hcv-RNA(—)为假阳。结果 67例经HC... 详细信息

目的分析化学发光微粒子免疫分析法(CMIA)检测anti-hcv阳性结果中假阳性的情况。方法选取本实验室测定anti-hcv结果为阳性的标本67例,用RT-PCR方法检测血清中hcv-RNA的载量,判断标准:hcv-RNA(+)为真阳,hcv-RNA(—)为假阳。结果 67例经hcv-RNA检测结果为hcv-RNA(+)者23例、hcv-RNA(—) 44例。结论应用本实验方法进行标本anti-hcv初筛检测时,其方法假阳性率较高,结果需进行补充或确认实验,临床诊断结论需结合其他临床相关资料。

关键词：丙型肝炎 anti-hcv 化学发光法

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罗氏诊断新一代Elecsys anti-hcv Ⅱ检测试剂上市

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中华检验医学杂志 2013年第3期36卷 260-260页

近日，罗氏诊断新一代Elecsys anti-hcv Ⅱ检测试剂上市会在上海召开，来自临床肝病领域、传染病实验室检测领域的专家以及罗氏诊断总部的专家等做了大会演讲。与会专家表示，如能早筛查、早诊断，丙肝治疗效果很好，约70％～80％的患... 详细信息

近日，罗氏诊断新一代Elecsys anti-hcv Ⅱ检测试剂上市会在上海召开，来自临床肝病领域、传染病实验室检测领域的专家以及罗氏诊断总部的专家等做了大会演讲。与会专家表示，如能早筛查、早诊断，丙肝治疗效果很好，约70％～80％的患者可通过规范治疗实现治愈。但其高隐匿、高漏诊、高慢性化的特点，使许多患者错过了最佳治疗时机。

关键词：罗氏诊断 anti-hcv 检测试剂上市 anti-hcv 实验室检测 hcv感染治疗效果

Phytochemical analysis of Berberis lyceum methanolic extract and its antiviral activity through the restoration of MAPK signaling pathway modulated by hcv NS5A

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Asian Pacific Journal of Tropical Biomedicine 2021年第3期11卷 132-140页

作者： Koloko Brice Landry Shafiq Azam Sidra Rehman Somayya Tariq Bushra Iqbal Mateen Abbas Dieudonné Massoma Lembè Bushra Ijaz Laboratory of Applied and Functional Genomic Centre of Excellence in Molecular BiologyUniversity of the PunjabLahorePakistan Department of Biosciences COMSATS University IslamabadIslamabadPakistan Quality Operations Laboratory University of Veterinary and Animal SciencesLahorePakistan Laboratory of Animal Biology and Physiology Faculty of ScienceUniversity of DoualaDoualaCameroon

Objective:To evaluate the antiviral activity and phytochemicals of selected plant extracts and their effect on the mitogen-activated protein kinase(MAPK)signaling pathway modulated by hepatitis C virus(hcv)nonstructural protein 5 A(NS5A).Methods:A total of ten plant extracts were initially screened for their toxicities against Hep G2 *** non-toxic plants were tested for their inhibitory effect on the expression of hcv NS5A at both m RNA and protein levels using real-time PCR and Western blotting assays,*** differential expression of the genes associated with MAPK pathway in the presence of NS5A gene and plant extract was measured through real-time ***,the identification of secondary metabolites was carried out by phytochemical and HPLC ***:The phytochemical profiling of Berberis lyceum revealed the presence of alkaloids,phenols,saponins,tannins,flavonoids,carbohydrates,terpenoids,steroids,and ***,quercetin,myricetin,gallic acid,caffeic acid,and ferulic acid were identified through HPLC *** methanolic extract of Berberis lyceum strongly inhibited hcv RNA replication with an IC50 of 11.44μg/m ***-PCR and Western blotting assays showed that the extract reduced the expression of hcv NS5A in a dosedependent *** lyceum extract also attenuated NS5A-induced dysregulation of the MAPK signaling ***:Our findings suggest that Berberis lyceum extract strongly inhibits hcv propagation by reducing hcv NS5A-induced perturbation of MAPK signaling.

关键词： anti-hcv hcv NS5A Berberis lyceum MAPK pathway Phytochemicals HPLC

Detection of hepatitis C virus core antigen for early diagnosis of hepatitis C virus infection in plasma donor in China

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World Journal of Gastroenterology 2007年第19期13卷 2738-2742页

作者： He-Qiu Zhang Shao-Bo Li Guo-Hua Wang Kun Chen Xiao-Guo Song Xiao-Yan Feng Department of Vaccine Engineering Beijing Institute of Basic Medical Sciences Beijing 100850 China Hunan Kingda Gene Ltd. Co. Changsha 410000 Hunan Province China

AIM： To evaluate the efficacy of a new hepatitis C virus （hcv） core antigen assay developed in China. METHODS： After the determination of hcv infection, 49 serial samples were selected from II regular plasma donors in 5 different plasma stations. To compare the performance of hcv core antigen detection and hcv PCR, these samples were genotyped, and each specimen was analyzed by ELISA for the detection of hcv core antigen and by qualitative hcv PCR. RESULTS： Among all of the sequential samples, the original 23 specimens were hcv RNA-negative, and 36 samples were hcv RNA-positive. Twenty-seven samples （75%） were hcv core antigen-positive from these hcv RNA-positive specimens. Conversely, 27 samples （93.2%） were found hcv RNA-positive in hcv core antigen- positive samples. Intervals between hcv RNA and hcv core antigen-positive, as well as between hcv core antigen-positive and hcv antibody-positive were 36.0 and 32.8 d, respectively. CONCLUSION： This hcv core antigen assay, developed in China, is able to detect much of anti-hcv-negative, hcv RNA-positive preseroconversion window period （PWP） plasma donations.

关键词： Hepatitis C virus Core antigen anti-hcv hcv RNA