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Variability of Deltamethrin-Resistant Metarhizium anisopliaeAggressive Strains Group to a Population of the Cossid Moth from Eucalyptus nitens

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Open Journal of Forestry 2018年第2期8卷 196-204页

作者： Pedro Romón Hardus Hatting Arturo Goldarazena Juan Carlos Iturrondobeitia Department of Zoology and Animal Cell Biology UPV-EHU University of Basque Country Leioa Spain FABI Forestry and Agricultural Biotechnology Institute TPCP Tree Protection Co-Operative Programme University of Pretoria Pretoria South Africa ELIB Earth and Life Institute Université catholique de Louvain Louvain Belgium

Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates tightly clustered into one or the other of two groups that diverged at 12%. Results suggested that certain genotypes of the fungus, that grouped together, were able to infect moth larvae while others did not. A fragment of 760 bp, which presents high homology with a host-adaptation related protein coding gene, distinguished between aggressive and non-aggressive isolates. Neither mycelial growth nor sporulation rate or presence of known virulence genes was correlated with mortality values. Some isolates, including the most aggressive isolate ARSEF2518, were compatible with deltamethrin. Deltamethrin treatment killed all the larvae after seven days whereas fungal and mixed treatments respectively reached the same mortality after 28 and 21 days.

关键词： Coryphodema tristis Metarhizium anisopliae Assays Compatibility with Deltamethrin RAPDs

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Isolation, Molecular Identification and under Lab Evaluation of the Entomopathogenic Fungi M. anisopliaeand B. bassianaagainst the Red Palm Weevil R. ferrugineusin Gaza Strip

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Advances in Microbiology 2017年第1期7卷 109-124页

作者： Abboud Y.El Kichaoui Bara’a A.Abu Asaker Mahmoud W.El-Hindi Biological Control Unit Department of BiotechnologyIslamic UniversityGazaPalestine

Plant diseases generate challenging problems in commercial, agriculture and pose real economic threats to both conventional and organic farming systems. The red palm weevil (Rhynchophorus ferrugineus) (RPW) is one of the most destructive pests of palms in the world. Nowadays, control methods revolve around treatments based on chemicals, biotechnological systems using semi-chemicals or the development of the sterile insect technique (hardly sustainable at this time) and biological control. Biological control as the use of natural microorganisms, extracted products from microorganisms or genetically improved to resist or eliminate of pathogens. Our aim was to evaluate the entomopathogenicity of indigenous Beauveria bassiana and Metarhizium anisopliae obtained in Gaza strip against larvae and adults of R. ferrugineus in order to identify indigenous strains potentially suitable for Red Palm Weevil biological control. B. bassiana & M. anisopliae were isolated from larvae and adult dead of RPW from different position of Gaza strip. Morphological analysis of the isolated fungi and molecular identification was determined using PCR technique. Also, the efficiency of the isolated fungi were evaluated under lab conditions and optimized as a biological agent. On the anther hand, the ability of treated RPW male to infect females is examined and calculated using Abbott's formula. Our results showed that the B. bassiana and M. anisopliae exhibited a good biological control agent against larvae and adults of RPW. The pathogenicity of the two most virulent isolates and the toxicity assay on larvae showed a highest mortality percentage nearly to 100% by 6 days after spraying the larvae with 3.4 × 108 spores/ml of B. bassiana. The mortality percentage reaches to 90% after spraying the larvae with 3.6 × 108 spores/ml of M. anisopliae. The mortality for the adults treated with pesticide arrives to 50% and the control group 10% at the same time. The results revealed that the infection of

关键词： B. bassiana M. anisopliae Red Palm Weevil Molecular Identification Biocontrol

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Metarhizium anisopliae *** Pr1A基因的克隆表达及抗血清研究

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生物技术 2006年第5期16卷 3-7页

作者：宋妍张世清黄俊生中国热带农业科学院环境与植物保护研究所热带作物生物技术国家重点实验室海南海口571101

采用RT-PCR方法从本实验室分离筛选到的金龟子绿僵小孢变种Metarhizium anisopliae ***中,扩增得到PrlA基因全长,此基因全长为1242bp,经Blastn分析此基因序列与***的PrlA基因(M73795)同源率为98%。以pET- 22b(+)为基础载体,构建pET-Prl... 详细信息

采用RT-PCR方法从本实验室分离筛选到的金龟子绿僵小孢变种Metarhizium anisopliae ***中,扩增得到PrlA基因全长,此基因全长为1242bp,经Blastn分析此基因序列与***的PrlA基因(M73795)同源率为98%。以pET- 22b(+)为基础载体,构建pET-PrlA重组表达载体,在大肠杆菌(***)BL 21(DE3)中进行表达。经SDS—PAGE分析,获得了约42kDa大小的重组目的蛋白,目的蛋白占表达总蛋白含量的63．2%。将表达的PrlA蛋白切胶回收后制备成抗原,免疫家兔4次后,采血收集抗血清,用ELISA测定效价为1/10000。结果表明,获得的抗体可用于更进一步的研究,将有利于我们进一步了解***的侵染机理,弄清楚各Pr蛋白酶的作用方式和对寄主的选择优势,提高生防控制的有效性。

关键词： Metarhizium anisopliae var.anisopliae Pr1A基因克隆表达抗血清

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田间噻嗪酮与黄绿绿僵菌对褐飞虱的协同防治

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中山大学学报（自然科学版） 2005年第3期44卷 78-81,85页

作者：耿博闻张润杰中山大学生物防治国家重点实验室

在广州郊区稻田中用黄绿绿僵菌Metarhiziumanisopliaevar acridumBosenbergetStrand和低用量农药噻嗪酮(buprofezin)对褐飞虱NilaparvatalugensSt l进行了协同防治作用药效试验。试验验共设低用量噻嗪酮、高用量噻嗪酮、黄绿绿僵菌、l... 详细信息

在广州郊区稻田中用黄绿绿僵菌Metarhiziumanisopliaevar acridumBosenbergetStrand和低用量农药噻嗪酮(buprofezin)对褐飞虱NilaparvatalugensSt l进行了协同防治作用药效试验。试验验共设低用量噻嗪酮、高用量噻嗪酮、黄绿绿僵菌、l低用量噻嗪酮和黄绿绿僵菌混施、清水对照共5个喷雾处理,每处理3个重复小区(4m×5m),随机区组排列。在处理前1天、处理后的第2、6、11、16、21d分别调查褐飞虱每丛的飞虱数。结果表明,低用量噻嗪酮与黄绿绿僵菌混施可以有效的控制田间稻飞虱的种群密度,对成虫的防效在第16天、对若虫第21天时达到95%以上,其相对防效和虫口减退率都有明显升高并表现出明显的协同作用,这种协同作用对褐飞虱成虫的防治效果优于对褐飞虱若虫的防治效果。

关键词：黄绿绿僵菌Metarhizium anisopliae var.acridum Bosenberg et Strand 噻嗪酮褐飞虱Nilaparvata lugens Stal协同作用

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