检索结果-南通市图书馆

Regulatory effects of anandamide on intracellular Ca^(2+) concentration increase in trigeminal ganglion neurons

Neural Regeneration Research 2014年第8期9卷 878-887页

作者： Yi Zhang Hong Xie Gang Lei Fen Li Jianping Pan Changjin Liu Zhiguo Liu Lieju Liu Xuehong Cao Department of Anesthesiology Tongji Hospital Affiliated to Tongji Medical CollegeHuazhong University of Science and Technology Jingzhou Central Hospital Department of Physiology Tongji Medical CollegeHuazhong University of Science and Technology Department of Bioengineering Wuhan Institute of Engineering

Activation of cannabinoid receptor type 1 on presynaptic neurons is postulated to suppress neu- ~ ~ ~ 2＋ ~ ~ 2＋ rotransmlsslon by decreasing Ca reflux through high voltage-gated Ca channels. However, recent studies suggest that cannabinoids which activate cannabinoid receptor type 1 can increase neurotransmitter release by enhancing Ca2＋ influx in vitro. The aim of the present study was to investigate the modulation of intracellular Ca2＋ concentration by the cannabinoid receptor type 1 agonist anandamide, and its underlying mechanisms. Using whole cell voltage-damp and calcium imaging in cultured trigeminal ganglion neurons, we found that anandamide directly caused Ca2＋ influx in a dose-dependent manner, which then triggered an increase of intracellular Ca2＋ concentration. The cyclic adenosine and guanosine monophosphate-dependent protein kinase systems, but not the protein kinase C system, were involved in the increased intracellular Ca2＋concentration by anandamide. This result showed that anandamide increased intracellu- lar Ca2＋ concentration and inhibited high voltage-gated Ca2＋ channels through different signal transduction pathways.

关键词： nerve regeneration trigeminal ganglion neurons endocannabinoids anandamide can-nabinoid receptor type 1 voltage-dependent calcium channels vanilloid receptor patch-damp tech-nique calcium cyclic adenosine monophosphate protein kinase protein kinase C NIH grant neuralregeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Endocannabinoids anandamide and Its Cannabinoid Receptors in Liver Fibrosis after Murine Schistosomiasis

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2009年第2期29卷 182-186页

作者：刘红艳高潇段瑞娴阳乔张曜文程勇卫郭燕唐望先 Institute of Liver Diseases Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

This study examined endogenous carmabinoid （ECB）-anandamide （AEA） and its cannabinoid receptors （CBR） in mice liver with the development ofschistosomajaponicum. Mice were infected with schistosoma by means of pasting the cercaria onto their abdomens. Liver fibrosis was pathologically confirmed nine weeks after the infection. High performance liquid chromatography （HPLC） was employed to determine the concentration of AEA in the plasma of mice. Immunofluo-rescence was used to detect the expression of CBR1 and CBR2 in liver tissue. Morphological examination showed typical pathological changes, with worm tubercles of schistosoma deposited in the liver tissue, fibrosis around the worm tubercles and infiltration or soakage of inflammatory cells. Also, CBR1 and CBR2 were present in hepatocytes and hepatic sinusoids of the two groups, but they were obviously enhanced in the schistosoma-infected mice. However, the average optical density of CBR1 in the negative control and fibrosis group was 13.28±7.32 and 30.55±7.78, and CBR2 were 28.13±6.42 and 52.29±4.24 （P〈0.05）. The levels of AEA in the fibrosis group were significantly increased as compared with those of the control group. The concentrations of AEA were （0.37±0.07） and （5.67±1.34） ng/mL （P〈0.05）. It is concluded that the expression of endocannabinoids AEA and its cannabinoid receptor CBR were significantly increased in schistosoma-infected mice. Endogenous endocannabinoids may be involved in the development of schistosoma-induced liver fibrosis.

关键词： anandamide cannabinoid receptor liver fibrosis schistosoma japonicum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Quantification of anandamide,oleoylethanolamide and palmitoylethanolamide in rodent brain tissue using high performance liquid chromatographyelectrospray mass spectroscopy

引用

Journal of Pharmaceutical Analysis 2014年第4期4卷 234-241页

作者： Daniel J.Liput Eleftheria Tsakalozou Dana C.Hammell Kalpana S.Paudel Kimberly Nixon Audra L.Stinchcomb Department of Pharmaceutical Sciences College of PharmacyUniversity of KentuckyLexingtonKY 40536USA AllTranz LexingtonKY 40505USA Department of Pharmaceutical Sciences College of PharmacySouth CollegeKnoxvilleTN 37922USA Department of Pharmaceutical Sciences School of PharmacyUniversity of MarylandBaltimoreMD 21201USA

Reported concentrations for endocannabinoids and related lipids in biological tissues can vary greatly; therefore, methods used to quantify these compounds need to be validated. This report describes a method to quantify anandamide （AEA）, oleoylethanolamide （OEA） and palmitoylethanolamide （PEA） from rodent brain tissue. Analytes were exwacted using acetonitfile without further sample clean up, resolved on a C 18 reverse-phase column using a gradient mobile phase and detected using eleclmspmy ionization in positive selected ion monitoring mode on a single quadrupole mass spectrometer. The method produced high recovery rates for AEA, OEA and PEA, ranging from 98.1% to 106.2%, 98.5% to 102.2% and 85.4% to 89.5%, respectively. The method resulted in adequate sensitivity with a lower limit of quantification for AEA, OEA

关键词： Endocarmabinoids Acylethanolamides anandamide OEA PEA LC-MS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Endocannabinoid,anandamide,Induces Cannabinoid Receptor-Independent Cell Death in Renal Proximal Tubule Cells

引用

CellBio 2017年第4期6卷 35-55页

作者： Monika Schlosser Heike Loser Soren V.Siegmund Manuel Montesinos-Rongen Laura Bindila Beat Lutz David A.Barrett Sarir Sarmad Catharine A.Ortori Veronika Grau Melanie von Brandenstein Jochen W.U.Fries Institute of Urology University Hospital of CologneCologneGermany Institute of Pathology University Hospital of CologneCologneGermany Department of Internal Medicine I University of BonnBonnGermany Institute of Neuropathology University Hospital of CologneCologneGermany Institute of Physiological Chemistry Medical Centre of Johannes Gutenberg UniversityMainzGermany Centre for Analytical Bioscience School of PharmacyUniversity of NottinghamNottinghamUK Department of General and Thoracic Surgery Justus-Liebig-University GiessenGiessenGermany

Background: The endocannabinoid (EC) system is well characterized in the central nervous system but scarcely studied in peripheral organs. In this paper, we newly identify the effect of the EC anandamide (AEA) upon renal proximal tubule cells. Methods: Measurement of lactate dehydrogenase (LDH) release after treatment of primary renal proximal tubule cells (RPTEC) and renal carcinoma cell line (Caki-1) with AEA, arachidonic acid (AA), ethanolamide (EtAm), EC receptor CB1 antagonist (AM251), CB2 receptor antagonist (SR144528), TRPV1 receptor antagonist (capsazepine), degradation enzyme fatty acid amide hydrolase (FAAH) antagonist (URB597), antioxidants GSH-EE;Trolox, GSH depletor BSO, membrane cholesterol depletor (MCD), apoptosis inhibitor zVAD, necroptosis inhibitor Nec-1 or ferroptosis inhibitor Fer-1. Western blot and qRT-PCR analysis plus determination of reactive oxygen species (ROS) via H2-DCFDA were performed. Histology for EC enzymes, N-acetylphosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD) and FAAH, as well as the determination of physiological levels of ECs in human and rat renal tissue via liquid chromatography were conducted. Results: AEA both dose- and time-dependently induces cell death in RPTEC and Caki-1 within hours, characterized by cell blebbing, not influenced by blocking the described EC receptors by AM251, SR144528, capsazepine or FAAH by URB597 or MCD. Cell death is mediated via ROS. There is no difference found in the histology of the enzymes FAAH and NAPE-PLD in human renal tissue with interstitial nephritis. Blocking of apoptotic, necroptotic or ferroptotic cell death does not lead to a reduction in LDH release in vitro. Conclusion: The endocannabinoid anandamide induces cell death in renal proximal tubule cell in a time- and dose-dependent manner. This pathway is mediated via ROS and is independent of cannabinoid receptors, membrane cholesterol or FAAH activity.

关键词： anandamide Cell Death RPTEC Caki-1 Endocannabinoid Receptor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

anandamide对大鼠心室肌细胞电生理特性的影响

Anandamide对大鼠心室肌细胞电生理特性的影响

引用

作者：李威华中科技大学

学位级别：硕士

目的研究内源性大麻素样物质anandamide对大鼠心室肌细胞电生理特性的影响。方法应用全细胞膜片钳技术记录大鼠单个心室肌细胞的动作电位和膜电流并观察药物对其的作用。结果(1)anandamide(10-9M)可以明显缩短动作电位时程(APD50和APD9... 详细信息

目的研究内源性大麻素样物质anandamide对大鼠心室肌细胞电生理特性的影响。方法应用全细胞膜片钳技术记录大鼠单个心室肌细胞的动作电位和膜电流并观察药物对其的作用。结果(1)anandamide(10-9M)可以明显缩短动作电位时程(APD50和APD90),用药前后APD50为49.4±5.7 VS 33.9±4.6,panandamide(10-9-10-5M)对瞬时外向钾电流(Ito)有双向作用,即在高浓度时(10-5 M)能使Ito减小,它使Ito从22.5±1.3pA/pF减小到17.9±1.8pA/pF,减小幅度20.2±3.6%,panandamide(10-9-10-5M)对持续性延迟整流钾电流(IK)和内向整流钾电流(IK1)无作用.结论anandamide (10-9 M)能缩短动作电位时程,anandamide(10-9-10-5M)对瞬时外向钾电流(Ito)有双向作用,即在高浓度时(10-5 M)能使Ito减小,而在低浓度时(10-9 M)能使Ito明显增大,然而,它对IK和Ik1却无作用。

关键词： anandamide 全细胞膜片钳技术动作电位时程瞬时外向钾电流(Ito) 延迟整流钾电流(Ik) 内向整流钾电流(Ik1)

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

慢性间歇性低压低氧对内源性大麻素类物质anandamide心脏保护作用的影响

慢性间歇性低压低氧对内源性大麻素类物质anandamide心脏保护作用...

引用

作者：王三怡河北医科大学

学位级别：硕士

目的:大量研究表明,慢性间歇性低压低氧(Chronic intermittent hypobaric hypoxia, CIHH)具有明显的心脏保护作用,可增强心脏对缺血、缺氧的耐受性,减轻缺血/再灌注对心肌的损伤,促进缺血/再灌注后心脏舒缩功能的恢复,以及抗心律失常。... 详细信息

目的:大量研究表明,慢性间歇性低压低氧(Chronic intermittent hypobaric hypoxia, CIHH)具有明显的心脏保护作用,可增强心脏对缺血、缺氧的耐受性,减轻缺血/再灌注对心肌的损伤,促进缺血/再灌注后心脏舒缩功能的恢复,以及抗心律失常。有研究报道,CIHH可改变机体对某些药物的反应性,从而间接发挥作用。anandamide(AEA)是一种内源性大麻素物质,具有抗心肌缺血、抗心律失常等心脏保护作用。但不知CIHH对AEA心脏保护作用有何影响。本研究旨在利用大鼠离体心脏缺血/再灌模型,观察CIHH对AEA抗心肌缺血/再灌损伤保护作用的影响,并探讨其机制。方法:雄性成年Sprague-Dawlay(SD)大鼠随机分为5组:间歇性低氧14天组(CIHH14)、间歇性低氧28天组(CIHH28)、anandamide组(AEA)、间歇性低氧14天加AEA组(CIHH14+AEA)和对照组(CON)。CIHH组和CIHH+AEA大鼠置于低压氧舱,分别接受14天、28天模拟5000米海拔高度(PB=404 mmHg, PO2=84 mmHg)的低氧处理,每天6小时。AEA和CON组大鼠除了不接受低氧处理外,其它处理均与间歇性低氧组动物相同。大鼠麻醉固定,迅速开胸,取出心脏,应用Langendorff离体心脏灌流装置制备心肌缺血/再灌注模型,即首先稳定灌流30 min,停灌30 min(缺血),然后复灌60 min(再灌注)。将液体小囊插入左心室,室内压力通过压力换能器输入记录系统,分别描记离体大鼠心脏基础状态和缺血/再灌注状态下不同时间的心脏功能的变化。心功能指标包括:左室发展压(left ventricular systolic pressure, LVDP)、左室舒张末压(left ventricular end diastolic pressure, LVEDP)和左室压力最大变化速率(maximal positive and negative velocity of left ventrichlar pressure,±LVdp/dtmax)。对离体大鼠心脏,分别在缺血前给予不同的药物干预。⑴对AEA和CIHH14+AEA组大鼠离体心脏,在缺血前给予10 min的AEA(2 nM)预处理;⑵对部分CIHH14+AEA大鼠离体心脏,在给AEA前分别给予10 min的AEA CB2受体阻断剂AM630(10 nM)和CB1受体阻断剂AM251(10 nM)预处理;⑶对部分CON大鼠离体心脏在缺血前分别给予10 min的AM630(10 nM)和AM251(10 nM)预处理。实验过程中,留取各监测时间点心脏灌流漏出液(冠脉流量),利用分光光度计按照乳酸脱氢酶试剂盒方法测定其中乳酸脱氢酶(lactate dehydrogenase, LDH)含量。实验结束后,取下心脏,置于液氮中冰冻保存。通过黄嘌呤氧化酶法方法,测定左心室心肌中超氧化物岐化酶(superoxide dismudase, SOD)活力,通过硫代巴比妥酸法计算出丙二醛(malondialdehyde, MDA)含量。结果:⑴基础状态下,CIHH14、CIHH28、AEA、CIHH14+AEA和CON大鼠的LVDP分别为132.1±9.7、132.0±12.8、135.5±8.6、131.6±8.5和132.2±11.0;LVEDP分别为5.5±2.9、5.6±1.8、7.8±5.7、5.8±3.3和5.7±2.6;+LVdp/dtmax分别为3606.4±97.1、3616.8±109.2、3610.3±116.8、3586.6±90.4和3617.3±97.9;-LVdp/dtmax分别为2536.4±87.9、2612.0±46.4、2498.4±96.4、2480.4±63.0和2500.4±80.4,各组心功能参数间均无明显差异(P>0.05)。⑵缺血后、再灌注60 min时,CIHH28和CIHH14+AEA大鼠的LVDP恢复分别为52.7±6.5%和53.1±8.5%,两者间无显著差异(P>0.05),但明显好于CIHH14大鼠的11.2±5.9%、AEA大鼠的8.9±5.3%和CON大鼠的10.4±4.0%(P0.05),但明显好于CIHH14大鼠的14.5±10.2%、AEA大鼠的15.7±9.7%和CON大鼠的15.6±9.1%(P<0.05);+LVdp/dtmax的恢复在CIHH28和CIHH14+AEA大鼠分别为38.1

关键词：慢性间歇性低压低氧 anandamide 心脏保护 CB1受体 CB2受体缺血/再灌注大鼠

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

脂多糖和干扰素-γ拮抗吗啡,anandamide,和雌激素诱导的人心房内皮iNOS的表达(英文)

引用

Acta Pharmacologica Sinica 2000年第5期21卷 23-27页

作者： Caterina FIMIANI,Harold MAGAZINE,Ingeborg D WELTERS,Thomas V BILFINGER,Felice SAL-SANO,Else TONNESEN,George B STEFANO Neuroscience Research Institute State University of New York College at Old Westbury Old Westbury NY 11568 USA Neuroscience Research Institute State University of New York College at Old Westbury Old Westbury NY 11568 USA Division of Cardiothoracic Surgery Neuroscience Research Institute Department of Surgery State University of New York College at Stony Brook Stony Brook NY 11794 USA Clinical Medicine Department La Sapienza University Rome State University of New York Italy Department of Anesthesia Aarhus Kommunehospital Denmark Neuroscience Research Institute State University of New York College at Old Westbury Old Westbury NY 11568 USA Division of Cardiothoracic Surgery College at Old Westbury Department of Surgery State University of New York College at Stony Brook Stony Brook NY 11794 USA Old Westbury NY 11568 USA Queens College and Graduate School of the City University of New York Flushing New York 11367 USA

AIM: To determine whether inducible nitric oxide syn-thase (iNOS) stimulation of human atrial fragments can be diminished by the naturally occurring signal molecules, such as morphine, anandamide, and estrogen . The use of iNOS as an indicator is justified since it has been associated with initiation of various types of cellular damage either directly or indirectly. METHODS: Western blots were performed on control and drug-exposed atrial tissue before and after lipopolysaccharide (LPS) and interferon-γ (IFN-γ) exposure. RESULTS: Preincubation of the tissue with morphine, anandamide or estrogen prior to, but not after, the addition of LPS + IFN-γ, blocked iNOS expression. The nitric oxide donor SNAP also blocked iNOS induction while preincubation of atrial fragments with an inhibitor of NOS, L-NAME, prior to morphine or anandamide exposure, restored LPS + IFN-γ induction of iNOS. CONCLUSION : These data suggest a direct regulatory link at the transcriptional level between constitutive (c) NOS and iNO

关键词：一氧化氮吗啡 anandamide 雌激素类一氧化氮合酶人类心房内皮

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cannabinoids and endocannabinoids as therapeutics for nervous system disorders:preclinical models and clinical studies

引用

Neural Regeneration Research 2024年第4期19卷 788-799页

作者： R.Scott Duncan Sean M.Riordan Matthew C.Gernon Peter Koulen Department of Ophthalmology School of MedicineUniversity of Missouri Department of Biomedical Sciences School of MedicineUniversity of Missouri

Cannabinoids are lipophilic substances derived from Cannabis sativa that can exert a variety of effects in the human *** have been studied in cellular and animal models as well as in human clinical trials for their therapeutic benefits in several human *** of these include central nervous system(CNS)diseases and dysfunctions such as forms of epilepsy,multiple sclerosis,Parkinson’s disease,pain and neuropsychiatric *** addition,the endogenously produced cannabinoid lipids,endocannabinoids,are critical for normal CNS function,and if controlled or modified,may represent an additional therapeutic avenue for CNS *** review discusses in vitro cellular,ex vivo tissue and in vivo animal model studies on cannabinoids and their utility as therapeutics in multiple CNS *** addition,the review provides an overview on the use of cannabinoids in human clinical trials for a variety of CNS *** and endocannabinoids hold promise for use as disease modifiers and therapeutic agents for the prevention or treatment of neurodegenerative diseases and neurological disorders.

关键词： anandamide cannabidiol cannabinoid endocannabinoid epilepsy multiple sclerosis neurodegeneration neuroprotection tetrahydrocannabinol

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

人类淋巴细胞中anandamide水解酶浓度的降低与流产之间的关系

引用

国际生殖健康/计划生育杂志 2001年第2期20卷 122-123页

作者：于新蕊

内源性大麻素类物质如anandamide被认为对怀孕和胚胎发育有不良影响,在初步试验中已经验证anandamide水解酶的活性与防止子宫内anandamide水平过高及妊娠失败或女性不孕相关。本文旨在探讨是否能用该降解酶的活性改变来预测孕妇的流产... 详细信息

内源性大麻素类物质如anandamide被认为对怀孕和胚胎发育有不良影响,在初步试验中已经验证anandamide水解酶的活性与防止子宫内anandamide水平过高及妊娠失败或女性不孕相关。本文旨在探讨是否能用该降解酶的活性改变来预测孕妇的流产情况。方法用特异的放射色谱法评估50例妊娠6～11周的健康孕妇外周淋巴中水解酶的活性。通过特异多克隆抗体的ELISA测定蛋白水平下的酶的表达。在进一步的研究中,测

关键词：淋巴细胞白细胞水解酶 anandamide 人类

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

内源性大麻素anandamide与非甾体类抗炎药布洛芬在急性炎症痛中的局部协同作用

引用

中国疼痛医学杂志 2006年第4期12卷 217-217页

作者：王宁

四氢大麻酚是大麻的主要活性成分,它通过结合CB1和CB2受体(G蛋白耦联受体)而发挥作用。anandamide是一种内源性四氢大麻酚,它主要通过作用于外周CBl受体来降低疼痛。它在体内会被脂肪酸氨基水解酶(FAAH)降解,非甾体类抗炎药(NSAID)如布... 详细信息

四氢大麻酚是大麻的主要活性成分,它通过结合CB1和CB2受体(G蛋白耦联受体)而发挥作用。anandamide是一种内源性四氢大麻酚,它主要通过作用于外周CBl受体来降低疼痛。它在体内会被脂肪酸氨基水解酶(FAAH)降解,非甾体类抗炎药(NSAID)如布洛芬能够抑制FAAH的活性,因此NSAID可以调节anandamide的外周镇痛作用。但是关于大麻与NSAID在外周的协同作用的报道较少。最近,加拿大蒙特利尔大学的Guindon研究小组进行了有关的实验性研究。[第一段]

关键词： anandamide 非甾体类抗炎药协同作用大麻素布洛芬内源性 anandamide 炎症痛外周镇痛作用 CB2受体

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：