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Journal of Biomedical Science and Engineering 2009年第7期2卷 532-537页

作者： Maryam Shojaei Abdolreza Mirmohseni Maryam Farbodi 不详

The aim of the present investigation was to develop a biosensor for the detection of amino acids, Leucine, Isoleucine and Valine based on a quartz crystal nanobalance. leucine (Leu), isoleucine (Ile), and valine (Val) were selectively determined by quartz crystal nanobalance (QCN) sensor in conjunction with net analyte signal (NAS)-based method called HLA/GO. An orthogonal design was applied for the formation of calibration and prediction sets including Leu, Ile and Val compounds. The selection of the optimal time range involved the calculation of the net analyte sig-nal regression plot in any considered time window for each test sample. The searching of a region with maximum linearity of NAS regression plot (minimum error indicator) and minimum of PRESS value was carried out by applying a moving window strategy. On the base of obtained results, the differences on the adsorption profiles in the time range between 1 and 300 s were used to determine mixtures of compounds by HLA/GO method. The results showed that the method was successfully applied for the determina-tion of Leu, Ile and Val.

关键词： Quartz Crystal Nanobalance Net analyte Signal Leucine Isoleucine Valine HLA/GO Method

自配analyte钾钠分析仪标准液及参比液

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水电医学杂志 1994年第3期 42-42页

作者：丛飞毕淑华张丽华姜兆远东北电业中心医院

analyte是美国Beker公司生产的钾钠离子分析仪器,采用流动直接选择电极对血清,血浆,全血及尿样等的分析仪器。本文介绍了自配该仪器的高标准和低标准及参比液的方法,解决进口试剂费用高,购买不方便等困难。自配试剂经临床四年多应用,取... 详细信息

analyte是美国Beker公司生产的钾钠离子分析仪器,采用流动直接选择电极对血清,血浆,全血及尿样等的分析仪器。本文介绍了自配该仪器的高标准和低标准及参比液的方法,解决进口试剂费用高,购买不方便等困难。自配试剂经临床四年多应用,取得满意效果,现总结报告如下。

关键词：参比液 EL 标准液 analyte 电解质分析仪分析仪器钠离子测定结果

Current and future opportunities for liquid biopsy of circulating biomarkers to aid in early cancer detection

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Journal of Cancer Metastasis and Treatment 2022年第1期8卷 334-344页

作者： Susan H.Gawel Laurel Jackson Nicolette Jeanblanc Gerard J.Davis Abbott Diagnostics Division Abbott LaboratoriesAbbott ParkIL 60064USA

Early diagnosis of cancer can significantly improve treatment and survival *** and tissue biopsy are the gold standard diagnostic approaches but are costly,invasive,and often unable to detect early-stage *** past decade has marked an acceleration in the discovery and development of liquid biopsy tests for aiding in the detection of various types of tumor markers in non-tissue samples,such as *** biopsy markers include circulating tumor cells,as well as tumor cell fragments,nucleic acids,and *** biopsy may be useful in screening patients considered to be at high risk of developing cancer,for refining diagnosis when combined with other test results,and for early detection of *** in big data analytics,informatics,and artificial intelligence will make it possible to combine patient history,clinical data,and liquid biopsy marker profiles to achieve more accurate and earlier *** this review,we summarize the current use of liquid biopsy in cancer care,including the development of multi-analyte panels to improve diagnostic accuracy and detect several cancer types in a single *** highlight recent advances for potential future applications of liquid biopsy to aid in the diagnosis of early-stage lung *** also discuss the opportunities and challenges of integrating liquid biopsy into current algorithms for cancer screening and diagnosis.

关键词： Cancer early detection diagnostic algorithm biomarkers blood serum analyte

Detecting Concentration of analytes with DETECHIP: A Molecular Sensing Array

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Journal of Sensor Technology 2013年第3期3卷 94-99页

作者： Hannah Johnke Gary Batres Mark Wilson Andrea E. Holmes Sharmin Sikich Department of Chemistry Doane College Crete USA

DETECHIP is a detection system made of various sensors that has been shown to detect and discriminate between small molecules of interest, including various illicit and over-the-counter drugs. Previously, detection was normalized to a single concentration of analyte. Now this detection assay can detect concentration differences in analytes via red, green, and blue color value changes and shifts in the UV-Vis spectra of the assay. To determine the concentrations differences, the exposed assays were scanned on a flatbed scanner and the images were analyzed for individual RGB values with a custom macro in ImageJ, an image analysis program. Increasing concentrations of the analyte resulted in greater differences in color values between control and analyte wells. These differences showed a linear relationship to concentration change, some with correlation coefficients greater than 98%. This work expands the capability of DETECHIP to give information about the concentration of analyte when the analyte identity is known.

关键词： Colorimetric Arrays Sensors analyte Concentration RGB Analysis Drug Detection

Capture Reagent and Strategy for Retrieving Albumin-Bound Ligands from Plasma

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Advances in Biological Chemistry 2019年第3期9卷 110-134页

作者： Megan M. Koslen Matthew W. Eskew Vincent Pinkert Huyen Hoang Fidelis Manyanga William L. Dean Jonathan B. Chaires Albert S. Benight Department of Chemistry Portland State University Portland Oregon USA James Graham Brown Cancer Center University of Louisville Louisville Kentucky USA Department of Physics Portland State University Portland Oregon USA

A capture strategy is described and demonstrated for retrieving ligand entities in plasma that bind Human Serum Albumin. The method has applications for both exogenous and endogenous ligands. Exogenous ligands include drug candidates, performance enhancing drugs and toxic nerve agents that also interact quite strongly with HSA. Endogenous ligands are natural circulating compounds whose abundance corresponds to normal hemostasis or elevated levels that could be disease-specific molecular biomarkers. Melting curves of plasma solutions measured by differential scanning calorimetry produce “so-called” plasma thermograms that are physical signatures of the plasma solution. Patterns displayed by thermograms can be sensitive indicators of the presence of abnormal levels of exogenous and endogenous ligand components. Effects of ligand interactions on thermodynamic stability of proteins in plasma that they bind, primarily HSA, manifest on the plasma thermogram. The capture strategy is demonstrated for HSA binding in plasma of four “ideal” ligands of different types. The particular ligands were naproxen, bromocresol green, short double stranded and single strand DNA. Thermogram shapes and features were sensitive to the presence of ligands as thermograms of mixtures of plasma and HSA with these ligands were significantly different than thermograms of plasma or HSA alone. These results demonstrated directly that significant perturbations of plasma thermograms corresponded to ligand interactions with HSA in plasma.

关键词： Human Serum Albumin (HSA) Differential Scanning Calorimetry (DSC) Protein Thermodynamic Stability analyte Capture

Quantitation of Genetox Impurities Using a Surrogate Standard Approach

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Journal of Analytical Sciences, Methods and Instrumentation 2013年第3期3卷 167-172页

作者： Heather Wang Regina Nardi Yuri Bereznitski Roy Helmy David J. Waterhouse Analytical Chemistry Merck Research Laboratories Merck Sharp & Dohme Corp. Rahway USA.

With the ever increasing complexity of active pharmaceutical ingredient (API) preparations, more potential genotoxic impurities (PGI’s) are being observed. It is thus necessary to determine if these PGI’s are present in the final API’s, and if they are present, to ensure the levels are acceptable for any clinical uses. For PGI’s that have authentic standards available, quantitation can be accomplished in a straightforward manner. However, for PGI’s that are expected to form through rearrangements or side reactions, authentic standards may not be readily available, significantly complicating the analysis. In this study we describe a surrogate standard approach for quantifying PGI’s that allows for relative response factor calculations of PGI species utilizing both gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS).

关键词： Potential Genotoxic Impurity (PGI) Active Pharmaceutical Ingredient (API) Mass Spectrometry Surrogate Standard analyte Relative Response Factor (RRF)