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Community analysis of ammonia-oxidizing bacteria in activated sludge of eight wastewater treatment systems

Journal of Environmental Sciences 2010年第4期22卷 627-634页

作者： Xiaohui Wang Xianghua Wen Craig Criddle George Wells Jie Zhang Yin Zhao Department of Environmental Science and Engineering Tsinghua University Beijing 100084 China. Department of Civil and Environmental Engineering Jerry Yang & Akiko Yamazaki Environment & Energy Building Stanford University Stanford CA94305-4020 USA Beijing Drainage Group Co. Ltd. Beijing 100084 China

We investigated the communities of ammonia-oxidizing bacteria （AOB） in activated sludge collected from eight wastewater treatment systems using polymerase chain reaction （PCR） followed by terminal restriction fragment length polymorphism （T-RFLP）, cloning, and sequencing of the α-subunit of the ammonia monooxygenase gene （amoa）. The T-RFLP fingerprint analyses showed that different wastewater treatment systems harbored distinct AOB communities. However, there was no remarkable difference among the AOB T- RFLP profiles from different parts of the same system. The T-RFLP fingerprints showed that a full-scale wastewater treatment plant （WWTP） contained a larger number of dominant AOB species than a pilot-scale reactor. The source of influent affected the AOB community, and the WWTPs treating domestic wastewater contained a higher AOB diversity than those receiving mixed domestic and industrial wastewater. However, the AOB community structure was little affected by the treatment process in this study. Phylogenetic analysis of the cloned amoa genes clearly indicated that all the dominant AOB in the systems was closely related to Nitrosomonas spp. not to Nitrosospira spp. Members of the Nitrosomonas oligotropha and Nitrosomonas communis clusters were found in all samples, while members of Nitrosomonas europaea cluster occurred in some systems.

关键词： activated sludge ammonia-oxidizing bacteria T-RFLP amoa gene wastewater treatment plant

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Microbial Populations,Activity and gene Abundance in Tropical Vertisols Under Intensive Chemical Farming

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Pedosphere 2016年第5期26卷 725-732页

作者： Kopparapu APARNA Desiraju Lakshmi Narsimha RAO Dananjeyan BALACHANDAR Indian Institute of Soil Science Nabi BaghBhopal 462038(India) Tamilnadu Agricultural University Coirnbatore 641003(India)

There are increasing concerns on the environmental impacts of intensive chemical agriculture. The effect of high agrochemical inputs used in intensive chemical farming was assessed on soil microbiological, molecular and biochemical properties in tropical Vertisols in India. Farm field sites under normal cultivation of arable crops using high inputs of fertilizers and pesticides in chili （Capsicum annum L., 5.0× dose for fertilizers and 1.5× dose for pesticides over normal inputs） and black gram （Vigna mungo L. Hepper, 2.2× dose for fertilizers and 2.3× dose for pesticides over normal inputs） were compared with adjacent sites using normal recommended doses. Organic carbon and basal respiration showed no response to high inputs of fertilizers and pesticides in soils of both crops. Labile carbon decreased by 10% in chili soils and increased by 24% in black gram soils under high input farming system. The proportion of soil labile carbon as a fraction of soil organic carbon was unaffected by high inputs. The labile carbon mineralization coefficient （qMLc） increased by 50.0% in chili soils, indicating that the soil microorganisms were under stress due to high agochemical inputs, whereas qMLc decreased by 36.4% in black gram soils. Copiotrophs increased due to high inputs in soils of both chili （63.1%） and black gram （47.1%）. Oligotrophs increased by 10.8% in black gram soils but not in chili soils. The abundance of amoa gene reduced by 39.3% in chili soils due to high inputs and increased significantly by 110.8% in black gram soils. β-Glucosidase also increased by 27.2% and 325.0%, respectively. Acid phosphatase activity reduced by 29.2% due to high inputs in chili soils and increased by 105.0% in black gram soils. The use of high agrochemical inputs thus had adverse consequences on biological health in chili but not in black gram soils. In soils cultivated with black gram, the moderating effect of cultivating legumes and their beneficial effect on soil health were eviden

关键词： amoa gene copiotrophs high agrochemical input legumes oligotrophs soil enzymes

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Environmental similarity is more important than distance in the community structuring processes of ammonia-oxidizing archaea in agricultural soils

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Pedosphere 2023年第6期33卷 819-827页

作者： Dorsaf KERFAHI Yu SHI Baozhan WANG Hokyung SONG Haiyan CHU Jonathan M.ADAMS School of Natural Sciences Department of Biological SciencesKeimyung UniversityDaegu 42601Korea State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475001China State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China Key Lab of Miboyrgriculural Eonment MintryfgrculureCllege of Life SciencesNanjing Agricultural Univery Nanjing 21008China Department of Earth and Environmental Sciences The University of ManchesterManchester M139PLUK University of Chinese Academy of Sciences Beijing 101408China School of Geography and Oceanography Nanjing UniversityNanjing 210008China

Ammonia-oxidizing archaea(AOA) are important in converting ammonia into nitrate in soils. While many aspects of their community structure have been studied, the relative importance of stochastic versus deterministic processes has poorly been understood. We compared AOA communities across the North China Plain, targeting the amoa gene. A phylogenetic null modelling approach was used to calculate the beta nearest taxon index to quantify the influence of stochastic and deterministic processes. We found that spatial distance between samples predicted the perceived processes involved in community structuring, with stochastic processes dominating at local scales. At greater distances, stochasticity became weaker. However, soil pH, which was also the strongest determinant of AOA community, was a much stronger predictor of community structuring, leaving the distance effect redundant as an explanation of community structuring processes. The communities of AOA differing by less than 1 pH unit differed mainly stochastically in terms of operational taxonomic unit composition. At larger pH differences, deterministic processes based on heterogeneous selection between clades became increasingly dominant. It appears that AOA community composition is largely determined by the environment. However, very similar pH environments are the exception. In environments with very close pH values, stochastic effects dominantly cause differences in community composition, whether spatially near or far.

关键词： amoa gene ammonia-oxidizing bacteria assembly processes beta nearest taxon index community structure operational taxonomic unit

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Invasive effects of the exotic plant *** on soil biogeochemical processes and related microbial community

Invasive effects of the exotic plant *** on soil biogeochemi...

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第三届全国生物入侵大会

作者： Xiao-fei Lu,Wan-xue Liu,Yu-jing Ren,Liang Chen,Chu-yun Long,Fang-hao Wan~* State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100081,China

Plant invasions have dramatic effects on native ecosystem structure and *** microbial diversity changes during the impacts of exotic plants invasion have been extensively examined,the shifts in diversity will not necessarily explain the ability of soil microbes to perform biogeochemical *** this study,we examined how microbial functional genes,together with their corresponding biogeochemical processes,respond to invasions of the exotic plant ***. Using terminal restriction fragment length polymorphism(T-RFLP),and quantitative polymerase chain reaction(qPCR),we compared the community structure and microbial abundance shifts in the rhizosphere and bulk soil associated with ***,comparing treatments in which it was either growing alone or together with native *** qPCR results showed that abundance of amoa and nosZ gene in the rhizosphere soil of *** changed more in bulk soil of native plants,both in monoculture and grown ***,T-RFLP results also showed that gene diversity shifts were more significant in ***’s rhizosphere *** results indicated that *** influences the belowground ecosystem in part through altered abundance of functional genes,rather than simply through changes in specific ***,the abundance and composition difference of microorganisms in soil could be characterized into significantly correlated factors with the altered storage and activity rates of nutrients in soil,particularly available nutrient and biogeochemical activity. Overall,these results suggest that *** would favor itself with more suitable biogeochemical cycle effects in the soil ecosystem by modifying the microorganism community.

关键词： Plant invasion Biogeochemical activity amoa gene nosZ gene

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Impact of heavy metal and antibiotic on soil potential nitrification and diversity of ammonia oxidizing archaea and bacteria

Impact of heavy metal and antibiotic on soil potential nitri...

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中国土壤学会土壤环境专业委员会第二十次会议暨农田土壤污染与修复研讨会

作者： Liao Qiang Li Ming-zhu Dong Yuan-peng Wu Mi Meng Zi-lin Zhang Qian Liu Ai-ju School of Agricultural Engineering and Food Science Shandong University of Technology School of Resources and Environment Engineering Shandong University of Technology

To evaluate the influence of heavy metals and antibiotics on soil nitrification and ammonia oxidizing microbial communities, soil potential nitrification rates(PNRs) and diversity of ammonia oxidizing microbes were investigated in spiked soils with Cu and Sulfadizine(SDZ). Obvious decrease of soil PNRs was observed with the increase of Cu and SDZ concentrations in soils. Real-time fluorescence quota PCR and pyrosequencing were adopted to investigate the population and diversity of ammonia-oxidizing archaea(AOA) and bacteria(AOB) in soils contaminated with 200 mg/kg Cu and 5 mg/kg SDZ, respectively. Real-time fluorescence quota PCR results showed that ammonia oxidizing bacteria(AOB) and ammonia oxidizing archaea(AOA) were slight stimulated at gene level in both contaminated soils, which was different from response of PNRs. Pyrosequencing analysis indicated that 200 mg/kg Cu could improve AOA diversity but reduce AOB diversity, but 5 mg/kg SDZ caused a decrease of both AOA and AOB diversity. Microbial community’s analysis also found that both 200 mg/kg Cu and 5 mg/kg SDZ had a slight promotion on the relative abundance of Candidatus Nitrosocosmicus and Nitrososphaera, which were predominated clusters of AOA, while for AOB’s prevalent microorganisms, Nitrosospira and Nitrosovibrio, they were promoted by 200 mg/kg Cu and decreased by 5 mg/kg SDZ, respectively. So, it could be concluded that Cu and SDZ might had a different ecological effects mechanism on soil potential nitrification and ammonia oxidizing microbial communities.

关键词： Heavy metals Antibiotics Soil nitrification Ammonia oxidizing microbes amoa gene

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Communities and Quantitative Analysis of Ammonia-oxidizing Organisms in Pearl River Estuary Sediments

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Agricultural Science & Technology 2012年第10期13卷 2080-2083,2087页

作者：陈金全郑燕平姜丽晶王风平厦门大学海洋与环境学院福建厦门361005 国家海洋局第三海洋研究所福建厦门361005 上海交通大学生命科学技术学院上海200240

[Objective] This study aimed to investigate the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea in Pearl River Estuary sediment.[Method] Firstly,the amoa gene library was constructed;then based on that,the content and diversity of amoa genes of ammonia-oxidizing bacteria and ammonia-oxidizing archaea in Pearl River Estuary sediment were detected by using quantitative real-time polymerase chain reaction（Q-PCR）.[Result] The results of Q-PCR presented that ammonia-oxidizing archaea（AOA） were more abundant than ammonia-oxidizing bacteria（AOB） in the top of sediment cores,with ratios of AOA to AOB of 22 and 9 at the two *** suggested that ammonia-oxidizing archaea may play more important roles than ammonia-oxidizing bacteria in the process of ammonia oxidation in the Pearl River Estuary *** phylogenetic tree based on amoa gene sequences revealed that the amoa sequences of both AOA and AOB shared high similarity with the clones from uncultured *** the top sediment layer at site Q7,AOB amoa-like gene sequences were dominated by Nitrosomonas-like sequence types,which could be classified into five groups（clusters A,B,C,D and E）.Cluster A accounted for 72.1% of the *** the top sediment layer,the AOA amoa gene fell into two groups ＂water column/sediment＂ cluster（52.2%） and ＂soil/sediment＂ cluster（47.8%）.But in the bottom sediment layer of Q7,most of the AOA amoa sequences（93.3%） fell into ＂soil/sediment＂ cluster,and a little part（6.7%） fell into the ＂water/sediment＂ *** addition,the total amount of amoa genes in the bottom sediment was higher than that in top sediment.[Conclusion] This study helps to realize the cycle of nitrogen in Pearl River Estuary Region,and thus to provide theoretical support for the treatment of nitrogen eutrophication.

关键词： Pearl River Estuary sediment Ammonia oxidation amoa gene Q-PCR

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