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Cinnamicaldehyde regulates the expression of tight junction proteins and amino acid transporters in intestinal porcine epithelial cells

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Journal of Animal Science and Biotechnology 2018年第1期9卷 127-134页

作者： Kaiji Sun Yan Lei Renjie Wang Zhenlong Wu Guoyao Wu State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing 100193 China DadHank (Chengdu) Biotech Corp Sichuan China Department of Animal Science Texas A&M University College Station TX 77843 USA Department of Animal Nutrition and Feed Science China Agricultural University Be'jing 100193. China

Background： Cinnamicaldehyde（CA） is a key flavor compound in cinnamon essential oil possessing various bioactivities. Tight junction（TJ） proteins are vital for the maintenance of intestinal epithelial barrier function,transport, absorption and utilization of dietary amino acids and other nutrients. In this study, we tested the hypothesis that CA may regulate the expression of TJ proteins and amino acid transporters in intestinal porcine epithelial cells（IPEC-1） isolated from neonatal ***： Compared with the control, cells incubated with 25 μmol/L CA had increased transepithelial electrical resistance（TEER） and decreased paracellular intestinal permeability. The beneficial effect of CA on mucosal barrier function was associated with enhanced protein abundance for claudin-4, zonula occludens（ZO）-1, ZO-2, and ZO-3. Immunofluorescence staining showed that 25 μmol/L CA promoted the localization of claudin-1 and claudin-3 to the plasma membrane without affecting the localization of other TJ proteins, including claudin-4, occludin,ZO-1, ZO-2, and ZO-3, compared with the control cells. Moreover, protein abundances for rBAT, xCT and LAT2 in IPEC-1 cells were enhanced by 25 μmol/L CA, while that for EAAT3 was not ***： CA improves intestinal mucosal barrier function by regulating the distribution of claudin-1 and claudin-3 in enterocytes, as well as enhancing protein abundance for amino acid transporters rBAT, xCT and LAT2 in enterocytes. Supplementation with CA may provide an effective nutritional strategy to improve intestinal integrity and amino acid transport and absorption in piglets.

关键词： amino acid transporters Barrier function Cinnamicaldehyde Intestinal epithelial cells Tight junction proteins

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Effects of reducing dietary protein on the expression of nutrition sensing genes(amino acid transporters) in weaned piglets

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第6期16卷 496-502页

作者： Li WU Liu-qin HE Zhi-jie CUI Gang LIU Kang YAO Fei WU Jun LI Tie-jun LI Research Center of Healthy Breeding of Livestock and Poultry Hunan Engineering and Research Center of Animal and Poultry ScienceKey Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of Sciences

The effects of crude protein（CP） levels in the diet on the m RNA expression of amino acid（AA） transporters were studied in a 45-d trial. Eighteen piglets with an initial body weight（BW） of 9.57 kg were assigned to three groups（14%, 17%, and 20% CP in the diet） in a completely randomized design（six replicates per treatment）. Diets were supplemented with crystalline AA to achieve equal standardized ileal digestible contents of Lys, Met plus Cys, Thr, and Trp, and were provided ad libitum. After 45 d, all piglets were slaughtered to collect small intestine samples. Compared with the values in the 14% CP group, the expressions of ASCT2, 4F2 hc, and ATB0 m RNA in the jejunum were increased by 23.00%, 12.00%, 6.00% and 48.00%, 47.00%, 56.00% in the 17% and 20% CP groups, respectively. These results indicate that a 14% CP diet supplemented with crystalline AA may not transport enough AA into the body and maintain growth performance of piglets. However, a reduction of dietary 17% CP may reduce the excretion of nitrogen into the environment while supporting the development of piglets. Therefore, the 17% CP level is more suitable than 14% CP level.

关键词： Crude protein amino acid balance amino acid transporters

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The appropriate standardized ileal digestible tryptophan to lysine ratio improves pig performance and regulates hormones and muscular amino acid transporters in late finishing gilts fed low-protein diets

The appropriate standardized ileal digestible tryptophan to ...

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第七届中国畜牧科技论坛

作者： Ma wenfeng Zhang shihai Zeng xiangfang LIU xutong Xie Chunyuan Zhang guijie Qiao shiyan College of Animal Science and Technology Henan University of Science and Technology State Key Laboratory of Animal Nutrition China Agricultural University

This study investigated the effects of various standardized ileal digestible(SID)Trp to Lys ratios on the performance and carcass characteristics of late finishing gilts receiving low CP(9.6%) diets supplemented with crystalline *** gilts(89.1 ± 5.1 kg) were utilized in a dose-response study conducted for 35 *** Trp(0,0.1,0.2,0.4 or0.6 g/kg) was added to a corn-wheat bran basal diet providing SID Trp to Lys ratios of 0.12,0.15,0.18,0.21 or *** diet was fed to 6 pens of pigs with 3 gilts per *** the end of experiment,30 gilts(one pig per pen) were slaughtered to evaluate carcass traits and meat quality(BW = 121 kg).Increasing the SID Trp to Lys ratio increased ADG(linear and quadratic effect,Pamino acid transporter of sodium-coupled neutral amino acid transporter 2(SNAT2) in the Longissimus dorsi muscle(linear and quadratic effect,Pamino acid SNAT2 transporters through the up-regulation of hormones such as GH and IGF-1.

关键词： amino acid transporters gilts hormones low-protein performance tryptophan

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The amino acid transporter SLC6A14 in cancer and its potential use in chemotherapy

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Asian Journal of Pharmaceutical Sciences 2014年第6期9卷 293-303页

作者： Yangzom D.Bhutia Ellappan Babu Puttur D.Prasad Vadivel Ganapathy Department of Biochemistry and Molecular Biology Medical College of GeorgiaGeorgia Regents UniversityAugustaGA 30912USA

Tumor cells have an increased demand for glucose and amino acids to support their rapid growth,and also exhibit alterations in biochemical pathways that metabolize these *** across the plasma membrane is essential to feed glucose and amino acids into these tumor cell-selective metabolic *** of amino acids across biological membranes occurs via a multitude of transporters;tumor cells must upregulate one or more of these transporters to satisfy their increased demand for amino *** the amino acid transporters,SLC6A14 stands out with specific functional features uniquely suited for the biological needs of the tumor *** transporter is indeed upregulated in tumors of epithelial origin,including colon cancer,cervical cancer,breast cancer,and pancreatic *** normal cells express this transporter only at low levels,blockade of this transporter should lead to amino acid starvation selectively in tumor cells,thus having little effect on normal *** offers a novel,yet logical,strategy for the treatment of cancers that are associated with upregulation of *** addition,a variety of amino acid-based prodrugs are recognized as substrates by SLC6A14,thus raising the possibility that anticancer drugs can be delivered into tumor cells selectively via this transporter in the form of amino acid *** strategy allows exposure of SLC6A14-positive tumor cells to chemotherapy with minimal off-target *** conclusion,the amino acid transporter SLC6A14 holds great potential not only as a direct drug target for cancer therapy but also for tumor cell-selective delivery of anticancer drugs.

关键词： Essential amino acids Glutamine addiction amino acid transporters SLC6A14 Prodrugs Cancer therapy

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