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Proteomics-Based Analysis of Phalaenopsis amabilisin Response toward CymbidiumMosaic Virus and/or OdontoglossumRingspot Virus Infection

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American Journal of Plant Sciences 2013年第9期4卷 1853-1862页

作者： Tongfei Lai Yanfu Deng Pengchen Zhang Zhijuan Chen Feng Hu Qi Zhang Yihua Hu Nongnong Shi Research Centre of Plant Signaling School of Life and Environmental Sciences Hangzhou Normal University Hangzhou China.

Stress response at the protein level to viral infection in orchid plants has not been extensively investigated to date. To understand the proteomic basis of Phalaenopsis amabilis’s responses to Cymbidium Mosaic virus (CymMV), and/or Odontoglossum ring spot virus (ORSV), the total proteins were extracted from Phalaenopsis amabilis leaves infected with CymMV, ORSV, or both respectively. Differentially expressed proteins were identified by two-dimensional electrophoresis, and 27 of these proteins that had significant changes were further examined by mass spectrometry. Comparing CymMV-infected leaves with mock-inoculated ones, 2 proteins were significantly up-regulated, 9 were significantly down-regulated and 1 previously undetected protein was identified. 10 proteins were significantly up-regulated, 3 significantly down-regulated and 1 previously undetected protein was identified in ORSV-infected leaves. 6 proteins were significantly up-regulated and 9 significantly down-regulated proteins were found in co-infected leaves. These identified proteins are involved in disease resistance, stress response, transcriptional regulation, energy metabolism, protein modification and the previously unknown proteins were not involved with known protein pathways. Proteins significantly up-regulated were ATP sulfurylase, down-regulated proteins included glutamate decarboxylase isozyme 2, RNA polymerase alpha subunit and chloroplastic peptide deformylase 1A were proteins with similar alteration trend after all infection treatments. Significantly up-regulated were Thioredoxin H-type and down-regulated Cytosolic phosphoglycerate kinase I which were proteins that have been shown to be specifically regulated by the infection with CymMV. Significantly up-regulated were proteins like Rubisco large subunit, Triosephosphate isomerase, NADP-specific isocitrate dehydrogenase and Cinnamoyl CoA reductase CCR2 by the infection of ORSV. Protein regulation in coinfected leaves followed a pattern simi

关键词： Phalaenopsis amabilis Two-Dimensional Electrophoresis Mass Spectrometry Cymbidium Mosaic Virus Odontoglossum Ringspot Virus

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Main Anatomy Characteristics in Cell Wall and Lignin Distribution of Bamboo Culms(Pseudosasa amabilis)

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Chinese Forestry Science and Technology 2012年第3期11卷 70-71页

作者： Yang Shumin Liu Xing'e Fei Benhua Jiang Zehui Yang Xi Shan Haibin International Center for Bamboo and Rattan

The anatomy characteristics of cell wall and lignin distribution in different anatomical regions for 12-month-old bamboo species Pseudosasa amabilis was *** lignin distribution was studied by means of optical microscope and confocal laser scanning microscope and the color reaction. In addition,the distribution of lignin in different tissues(fiber,parenchyma and vessel) was investigated by visible-light microspectrophotometry coupled with the Wiesner and Maule *** cell walls of different tissues were all lignified and the lignin was widely distributed in different tissues, in which there was a difference between tissues and anatomical regions in lignin *** lignin and syringyl lignin unit could be found in cell wall of fiber,parenchyma and vessel through Wiesner and Maule reaction and the absorbance peak of spectrum. There was no regular variation in lignin content with different radial location of culms and different location in fiber *** lamellation of the fiber cell wall was generally had as alternating broad and narrow layers with alternating low and high concentration of lignin.

关键词： Pseudosasa amabilis characteristics of cell wall distribution of lignin visible-light microspectrophotometry

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墨兰和蝴蝶兰镰刀菌病害的鉴定

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仲恺农业工程学院学报 2011年第2期24卷 5-8,24页

作者：施祖荣张云霞黄江华向梅梅仲恺农业工程学院农学院广东广州510225

对广东栽培墨兰(Cymbidium sinense (Andr.) Willd.)和蝴蝶兰(Phalaenopsis amabilis Blume)的镰刀菌病害进行了鉴定.经症状观察、病原菌分离培养与回接、病原菌形态观察和内部转录间隔区(Internal transcribed space,ITS)、延伸因子-1α(Elongation factor-1α,EF-1α)序列分析,鉴定出墨兰茎腐病、蝴蝶兰茎基腐病和蝴蝶兰花梗腐烂病等3种镰刀菌病害,其病原菌分别为尖孢镰刀菌(Fusarium oxysporum)、茄镰刀菌(***)和藤仓镰刀菌(***).其中,蝴蝶兰花梗腐烂病(***)为兰花上首次报道.

关键词：真菌病害墨兰(Cymbidium sinense (Andr.) Willd.) 蝴蝶兰(Phalaenopsis amabilis Blume) 镰刀菌(Fusarium)

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蝴蝶兰“大辣椒”花梗组织快繁技术研究

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林业科技通讯 2017年第5期 66-69页

作者：付镇芳何博马杰穆妮妮豆丽萍庆阳市农业科学研究院甘肃庆阳745000

以蝴蝶兰"大辣椒"(Phalaenopsis amabilis Big Chilli)花梗为外植体,研究不同培养基、生长调节物质对蝴蝶兰组培各个阶段产生的影响,建立蝴蝶兰"大辣椒"的离体快繁技术体系。结果表明0.1%的升汞消毒10min效果最佳,污染率最低可达到27.6... 详细信息

以蝴蝶兰"大辣椒"(Phalaenopsis amabilis Big Chilli)花梗为外植体,研究不同培养基、生长调节物质对蝴蝶兰组培各个阶段产生的影响,建立蝴蝶兰"大辣椒"的离体快繁技术体系。结果表明0.1%的升汞消毒10min效果最佳,污染率最低可达到27.69%,成活率80.75%;腋芽萌发最佳培养基为MS+6-BA 2.0mg/L+NAA 0.05mg/L+蔗糖30g/L+活性炭1g/L+琼脂6g/L,pH值5.6,萌发率82.99%;增殖最佳培养为MS+6-BA 5.0mg/L+NAA 0.1mg/L+蔗糖30g/L+活性炭1g/L+琼脂6g/L pH值5.6,增殖系数2.75,暗培养7d有利于增殖;添加了IBA 2.0mg/L的1/2 MS培养基上,生根最好。

关键词：蝴蝶兰 “大辣椒” Phalaenopsis amabilis 组织快繁花梗诱导

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